dbPTM

TBCD5_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	TBCD5_HUMAN
UniProt AC	Q92609
Protein Name	TBC1 domain family member 5
Gene Name	TBC1D5
Organism	Homo sapiens (Human).
Sequence Length	795
Subcellular Localization	Endosome membrane . Cytoplasmic vesicle, autophagosome . During starvation induced autophagy is relocalized from endosomal localization to LC3-positive autophagosomes.
Protein Description	May act as a GTPase-activating protein (GAP) for Rab family protein(s). May act as a GAP for RAB7A. Can displace RAB7A and retromer CSC subcomplex from the endosomal membrane to the cytosol; at least retromer displacement seems to require its catalytic activity. [PubMed: 19531583]
Protein Sequence	MYHSLSETRHPLQPEEQEVGIDPLSSYSNKSGGDSNKNGRRTSSTLDSEGTFNSYRKEWEELFVNNNYLATIRQKGINGQLRSSRFRSICWKLFLCVLPQDKSQWISRIEELRAWYSNIKEIHITNPRKVVGQQDLMINNPLSQDEGSLWNKFFQDKELRSMIEQDVKRTFPEMQFFQQENVRKILTDVLFCYARENEQLLYKQGMHELLAPIVFVLHCDHQAFLHASESAQPSEEMKTVLNPEYLEHDAYAVFSQLMETAEPWFSTFEHDGQKGKETLMTPIPFARPQDLGPTIAIVTKVNQIQDHLLKKHDIELYMHLNRLEIAPQIYGLRWVRLLFGREFPLQDLLVVWDALFADGLSLGLVDYIFVAMLLYIRDALISSNYQTCLGLLMHYPFIGDVHSLILKALFLRDPKRNPRPVTYQFHPNLDYYKARGADLMNKSRTNAKGAPLNINKVSNSLINFGRKLISPAMAPGSAGGPVPGGNSSSSSSVVIPTRTSAEAPSHHLQQQQQQQRLMKSESMPVQLNKGLSSKNISSSPSVESLPGGREFTGSPPSSATKKDSFFSNISRSRSHSKTMGRKESEEELEAQISFLQGQLNDLDAMCKYCAKVMDTHLVNIQDVILQENLEKEDQILVSLAGLKQIKDILKGSLRFNQSQLEAEENEQITIADNHYCSSGQGQGRGQGQSVQMSGAIKQASSETPGCTDRGNSDDFILISKDDDGSSARGSFSGQAQPLRTLRSTSGKSQAPVCSPLVFSDPLMGPASASSSNPSSSPDDDSSKDSGFTIVSPLDI

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Phosphorylation	------MYHSLSETR ------CCCCCCCCC	10.50	25159151
4	Phosphorylation	----MYHSLSETRHP ----CCCCCCCCCCC	18.99	23401153
6	Phosphorylation	--MYHSLSETRHPLQ --CCCCCCCCCCCCC	39.68	25159151
8	Phosphorylation	MYHSLSETRHPLQPE CCCCCCCCCCCCCHH	30.79	25159151
25	Phosphorylation	EVGIDPLSSYSNKSG CCCCCCCHHCCCCCC	32.39	26074081
26	Phosphorylation	VGIDPLSSYSNKSGG CCCCCCHHCCCCCCC	40.02	26074081
27	Phosphorylation	GIDPLSSYSNKSGGD CCCCCHHCCCCCCCC	16.94	26074081
28	Phosphorylation	IDPLSSYSNKSGGDS CCCCHHCCCCCCCCC	39.46	26074081
30	Ubiquitination	PLSSYSNKSGGDSNK CCHHCCCCCCCCCCC	44.53	-
31	Phosphorylation	LSSYSNKSGGDSNKN CHHCCCCCCCCCCCC	52.86	26074081
35	Phosphorylation	SNKSGGDSNKNGRRT CCCCCCCCCCCCCCC	53.64	26074081
42	Phosphorylation	SNKNGRRTSSTLDSE CCCCCCCCCCCCCCC	26.18	23927012
43	Phosphorylation	NKNGRRTSSTLDSEG CCCCCCCCCCCCCCC	21.36	23927012
44	Phosphorylation	KNGRRTSSTLDSEGT CCCCCCCCCCCCCCC	32.17	23927012
45	Phosphorylation	NGRRTSSTLDSEGTF CCCCCCCCCCCCCCC	34.06	25159151
48	Phosphorylation	RTSSTLDSEGTFNSY CCCCCCCCCCCCCHH	40.83	23927012
51	Phosphorylation	STLDSEGTFNSYRKE CCCCCCCCCCHHHHH	18.65	23927012
54	Phosphorylation	DSEGTFNSYRKEWEE CCCCCCCHHHHHHHH	23.32	23403867
55	Phosphorylation	SEGTFNSYRKEWEEL CCCCCCHHHHHHHHH	27.15	23403867
75	Acetylation	YLATIRQKGINGQLR HHHHHHHCCCCCCCC	52.49	164883
75	Ubiquitination	YLATIRQKGINGQLR HHHHHHHCCCCCCCC	52.49	-
125	Phosphorylation	NIKEIHITNPRKVVG CCEEEEECCCCCEEC	25.63	26074081
129	Ubiquitination	IHITNPRKVVGQQDL EEECCCCCEECCCCC	42.39	-
157	Acetylation	WNKFFQDKELRSMIE HHHHHCCHHHHHHHH	48.11	25953088
157	Ubiquitination	WNKFFQDKELRSMIE HHHHHCCHHHHHHHH	48.11	-
168	Acetylation	SMIEQDVKRTFPEMQ HHHHHHHHHHCCHHH	54.75	25953088
168	Ubiquitination	SMIEQDVKRTFPEMQ HHHHHHHHHHCCHHH	54.75	-
170	Phosphorylation	IEQDVKRTFPEMQFF HHHHHHHHCCHHHHH	37.51	22210691
281	Phosphorylation	KGKETLMTPIPFARP CCCEEEECCCCCCCH	22.60	27251275
310	2-Hydroxyisobutyrylation	QIQDHLLKKHDIELY HHHHHHHHHHCHHHH	55.19	-
422	Phosphorylation	KRNPRPVTYQFHPNL CCCCCCCEEEECCCC	17.81	28796482
423	Phosphorylation	RNPRPVTYQFHPNLD CCCCCCEEEECCCCH	14.93	28796482
431	Phosphorylation	QFHPNLDYYKARGAD EECCCCHHHHHHCHH	15.27	-
432	Phosphorylation	FHPNLDYYKARGADL ECCCCHHHHHHCHHH	9.61	22817900
433	Ubiquitination	HPNLDYYKARGADLM CCCCHHHHHHCHHHC	25.94	-
435	Methylation	NLDYYKARGADLMNK CCHHHHHHCHHHCCC	36.36	115918269
443	Phosphorylation	GADLMNKSRTNAKGA CHHHCCCCCCCCCCC	39.06	24719451
448	Ubiquitination	NKSRTNAKGAPLNIN CCCCCCCCCCCCCHH	58.64	-
456	Ubiquitination	GAPLNINKVSNSLIN CCCCCHHHHHHHHHH	43.15	21890473
456	Ubiquitination	GAPLNINKVSNSLIN CCCCCHHHHHHHHHH	43.15	2189047
458	Phosphorylation	PLNINKVSNSLINFG CCCHHHHHHHHHHCC	23.47	29514088
460	Phosphorylation	NINKVSNSLINFGRK CHHHHHHHHHHCCCC	24.10	25159151
467	Ubiquitination	SLINFGRKLISPAMA HHHHCCCCCCCCCCC	51.02	-
470	Phosphorylation	NFGRKLISPAMAPGS HCCCCCCCCCCCCCC	19.62	25159151
490	Phosphorylation	PGGNSSSSSSVVIPT CCCCCCCCCEEEECC	28.69	27251789
491	Phosphorylation	GGNSSSSSSVVIPTR CCCCCCCCEEEECCC	29.15	27251789
492	Phosphorylation	GNSSSSSSVVIPTRT CCCCCCCEEEECCCC	23.50	28555341
519	Methylation	QQQQRLMKSESMPVQ HHHHHHHHCCCCCCC	56.51	115980139
520	Phosphorylation	QQQRLMKSESMPVQL HHHHHHHCCCCCCCC	22.42	23401153
522	Phosphorylation	QRLMKSESMPVQLNK HHHHHCCCCCCCCCC	35.16	25159151
523	Sulfoxidation	RLMKSESMPVQLNKG HHHHCCCCCCCCCCC	3.11	21406390
532	Phosphorylation	VQLNKGLSSKNISSS CCCCCCCCCCCCCCC	48.19	24719451
534	Acetylation	LNKGLSSKNISSSPS CCCCCCCCCCCCCCC	55.73	25953088
537	Phosphorylation	GLSSKNISSSPSVES CCCCCCCCCCCCCCC	33.87	29255136
538	Phosphorylation	LSSKNISSSPSVESL CCCCCCCCCCCCCCC	42.33	29255136
539	Phosphorylation	SSKNISSSPSVESLP CCCCCCCCCCCCCCC	17.88	29255136
541	Phosphorylation	KNISSSPSVESLPGG CCCCCCCCCCCCCCC	40.23	29255136
544	Phosphorylation	SSSPSVESLPGGREF CCCCCCCCCCCCCCC	37.39	29255136
552	Phosphorylation	LPGGREFTGSPPSSA CCCCCCCCCCCCCCC	31.81	25159151
554	Phosphorylation	GGREFTGSPPSSATK CCCCCCCCCCCCCCC	30.49	19664994
557	Phosphorylation	EFTGSPPSSATKKDS CCCCCCCCCCCCCCC	35.67	29255136
558	Phosphorylation	FTGSPPSSATKKDSF CCCCCCCCCCCCCCC	45.81	29255136
559	Phosphorylation	TGSPPSSATKKDSFF CCCCCCCCCCCCCCC	27.98	27251275
560	Phosphorylation	GSPPSSATKKDSFFS CCCCCCCCCCCCCCC	40.34	29255136
561 (in isoform 2)	Phosphorylation	-	55.06	29743597
563	Phosphorylation	PSSATKKDSFFSNIS CCCCCCCCCCCCHHC	52.84	27251275
563 (in isoform 2)	Phosphorylation	-	52.84	29743597
564	Phosphorylation	SSATKKDSFFSNISR CCCCCCCCCCCHHCC	36.75	23401153
566	Phosphorylation	ATKKDSFFSNISRSR CCCCCCCCCHHCCCC	6.73	27251275
566 (in isoform 2)	Phosphorylation	-	6.73	29743597
567	Phosphorylation	TKKDSFFSNISRSRS CCCCCCCCHHCCCCC	31.10	22199227
570	Phosphorylation	DSFFSNISRSRSHSK CCCCCHHCCCCCCCC	28.58	23401153
572	Phosphorylation	FFSNISRSRSHSKTM CCCHHCCCCCCCCCC	31.20	30576142
574	Phosphorylation	SNISRSRSHSKTMGR CHHCCCCCCCCCCCC	33.10	29514088
576	Phosphorylation	ISRSRSHSKTMGRKE HCCCCCCCCCCCCCC	30.94	29514088
578	Phosphorylation	RSRSHSKTMGRKESE CCCCCCCCCCCCCCH	28.07	24247654
579	Phosphorylation	SRSHSKTMGRKESEE CCCCCCCCCCCCCHH	5.85	27251275
580	Phosphorylation	RSHSKTMGRKESEEE CCCCCCCCCCCCHHH	42.62	27251275
584	Phosphorylation	KTMGRKESEEELEAQ CCCCCCCCHHHHHHH	54.03	28192239
586	Phosphorylation	MGRKESEEELEAQIS CCCCCCHHHHHHHHH	76.73	27251275
589	Phosphorylation	KESEEELEAQISFLQ CCCHHHHHHHHHHHH	41.63	27251275
592	Phosphorylation	EEELEAQISFLQGQL HHHHHHHHHHHHHHH	3.97	27251275
593	Phosphorylation	EELEAQISFLQGQLN HHHHHHHHHHHHHHC	14.07	27732954
606	Phosphorylation	LNDLDAMCKYCAKVM HCCHHHHHHHHHHHH	2.81	27251275
615	Phosphorylation	YCAKVMDTHLVNIQD HHHHHHHHHCCCHHH	10.06	-
646	Ubiquitination	LAGLKQIKDILKGSL HHCHHHHHHHHHHHC	35.90	-
650	Ubiquitination	KQIKDILKGSLRFNQ HHHHHHHHHHCCCCH	46.95	-
658	Phosphorylation	GSLRFNQSQLEAEEN HHCCCCHHHHHHHHC	37.53	21712546
669	Phosphorylation	AEENEQITIADNHYC HHHCCCEEEECCCEE	15.14	24043423
675	Phosphorylation	ITIADNHYCSSGQGQ EEEECCCEECCCCCC	10.57	28796482
677	Phosphorylation	IADNHYCSSGQGQGR EECCCEECCCCCCCC	30.27	28796482
678	Phosphorylation	ADNHYCSSGQGQGRG ECCCEECCCCCCCCC	31.41	28796482
689	Phosphorylation	QGRGQGQSVQMSGAI CCCCCCCCEECCCCH	22.57	28555341
692	Sulfoxidation	GQGQSVQMSGAIKQA CCCCCEECCCCHHHC	3.60	21406390
693	Phosphorylation	QGQSVQMSGAIKQAS CCCCEECCCCHHHCC	14.16	29116813
697	Phosphorylation	VQMSGAIKQASSETP EECCCCHHHCCCCCC	39.00	27642862
700	Phosphorylation	SGAIKQASSETPGCT CCCHHHCCCCCCCCC	26.64	21815630
701	Phosphorylation	GAIKQASSETPGCTD CCHHHCCCCCCCCCC	48.80	28450419
703	Phosphorylation	IKQASSETPGCTDRG HHHCCCCCCCCCCCC	27.25	21815630
707	Phosphorylation	SSETPGCTDRGNSDD CCCCCCCCCCCCCCC	34.35	23090842
712	Phosphorylation	GCTDRGNSDDFILIS CCCCCCCCCCEEEEE	41.19	21815630
719	Phosphorylation	SDDFILISKDDDGSS CCCEEEEEECCCCCC	26.96	23090842
725	Phosphorylation	ISKDDDGSSARGSFS EEECCCCCCCCCCCC	28.02	28450419
726	Phosphorylation	SKDDDGSSARGSFSG EECCCCCCCCCCCCC	27.75	30576142
728	Methylation	DDDGSSARGSFSGQA CCCCCCCCCCCCCCC	43.14	115918273
730	Phosphorylation	DGSSARGSFSGQAQP CCCCCCCCCCCCCCC	15.64	23401153
732	Phosphorylation	SSARGSFSGQAQPLR CCCCCCCCCCCCCCC	31.52	25159151
740	Phosphorylation	GQAQPLRTLRSTSGK CCCCCCCCHHCCCCC	33.92	25159151
743	O-linked_Glycosylation	QPLRTLRSTSGKSQA CCCCCHHCCCCCCCC	30.00	28411811
743	Phosphorylation	QPLRTLRSTSGKSQA CCCCCHHCCCCCCCC	30.00	26699800
744	O-linked_Glycosylation	PLRTLRSTSGKSQAP CCCCHHCCCCCCCCC	34.76	28411811
744	Phosphorylation	PLRTLRSTSGKSQAP CCCCHHCCCCCCCCC	34.76	26699800
745	Phosphorylation	LRTLRSTSGKSQAPV CCCHHCCCCCCCCCC	45.53	26074081
748	Phosphorylation	LRSTSGKSQAPVCSP HHCCCCCCCCCCCCC	34.79	26074081
752	Phosphorylation	SGKSQAPVCSPLVFS CCCCCCCCCCCEEEC	5.90	27251275
754	Phosphorylation	KSQAPVCSPLVFSDP CCCCCCCCCEEECCC	22.85	26074081
759	Phosphorylation	VCSPLVFSDPLMGPA CCCCEEECCCCCCCC	30.73	26074081
765	Phosphorylation	FSDPLMGPASASSSN ECCCCCCCCCCCCCC	14.74	27251275
767	Phosphorylation	DPLMGPASASSSNPS CCCCCCCCCCCCCCC	31.64	26074081
769	Phosphorylation	LMGPASASSSNPSSS CCCCCCCCCCCCCCC	31.63	26074081
770	Phosphorylation	MGPASASSSNPSSSP CCCCCCCCCCCCCCC	33.57	28450419
771	Phosphorylation	GPASASSSNPSSSPD CCCCCCCCCCCCCCC	50.71	28450419
774	Phosphorylation	SASSSNPSSSPDDDS CCCCCCCCCCCCCCC	47.93	26657352
775	Phosphorylation	ASSSNPSSSPDDDSS CCCCCCCCCCCCCCC	47.34	28450419
776	Phosphorylation	SSSNPSSSPDDDSSK CCCCCCCCCCCCCCC	36.36	28450419
781	Phosphorylation	SSSPDDDSSKDSGFT CCCCCCCCCCCCCCE	46.40	26074081
782	Phosphorylation	SSPDDDSSKDSGFTI CCCCCCCCCCCCCEE	47.69	26074081
788	Phosphorylation	SSKDSGFTIVSPLDI CCCCCCCEEEECCCC	24.80	27251275
791	Phosphorylation	DSGFTIVSPLDI--- CCCCEEEECCCC---	18.85	25159151
796	Phosphorylation	IVSPLDI-------- EEECCCC--------		27251275
813	Phosphorylation	------------------------- -------------------------		27251275

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of TBCD5_HUMAN !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of TBCD5_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of TBCD5_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
GBRL2_HUMAN	GABARAPL2	physical	16189514
VPS35_HUMAN	VPS35	physical	22354992
VP26A_HUMAN	VPS26A	physical	22354992
VP26B_HUMAN	VPS26B	physical	22354992
VPS29_HUMAN	VPS29	physical	22354992
WAC2A_HUMAN	FAM21A	physical	22354992
MLP3A_HUMAN	MAP1LC3A	physical	22354992
GBRL2_HUMAN	GABARAPL2	physical	25416956
GBRL1_HUMAN	GABARAPL1	physical	25416956
VPS29_HUMAN	VPS29	physical	25416956
EIF1A_HUMAN	EIF1AD	physical	25416956

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of TBCD5_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions."; Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.; Sci. Signal. 2:RA46-RA46(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-522; SER-541 ANDSER-544, AND MASS SPECTROMETRY.	19690332 [Abstract]
"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach."; Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.; Anal. Chem. 81:4493-4501(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-522, AND MASSSPECTROMETRY.	19413330 [Abstract]
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-42; THR-45; SER-539 ANDSER-544, AND MASS SPECTROMETRY.	18669648 [Abstract]
"Kinase-selective enrichment enables quantitative phosphoproteomics ofthe kinome across the cell cycle."; Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R.,Greff Z., Keri G., Stemmann O., Mann M.; Mol. Cell 31:438-448(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-43 AND SER-554, AND MASSSPECTROMETRY.	18691976 [Abstract]
"Phosphoproteome of resting human platelets."; Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J.,Schuetz C., Walter U., Gambaryan S., Sickmann A.; J. Proteome Res. 7:526-534(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-554, AND MASSSPECTROMETRY.	18088087 [Abstract]
"Robust phosphoproteomic profiling of tyrosine phosphorylation sitesfrom human T cells using immobilized metal affinity chromatography andtandem mass spectrometry."; Brill L.M., Salomon A.R., Ficarro S.B., Mukherji M., Stettler-Gill M.,Peters E.C.; Anal. Chem. 76:2763-2772(2004). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-43; SER-44 AND THR-45,AND MASS SPECTROMETRY.	15144186 [Abstract]