dbPTM

WWTR1_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	WWTR1_MOUSE
UniProt AC	Q9EPK5
Protein Name	WW domain-containing transcription regulator protein 1
Gene Name	Wwtr1
Organism	Mus musculus (Mouse).
Sequence Length	395
Subcellular Localization	Nucleus . Cytoplasm . Concentrates along specific portions of the plasma membrane, and accumulates in punctate nuclear bodies. When phosphorylated is retained in cytoplasm by YWHAZ. Can be retained in the nucleus by MED15 (By similarity)..
Protein Description	Transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation. Regulates the nuclear accumulation of SMADS and has a key role in coupling them to the transcriptional machinery such as the mediator complex. Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition (By similarity)..
Protein Sequence	MNPSSVPHPLPPPGQQVIHVTQDLDTDLEALFNSVMNPKPSSWRKKILPESFFKEPDSGSHSRQSSTDSSGGHPGPRLAGGAQHVRSHSSPASLQLGTGAGAAGGPAQQHAHLRQQSYDVTDELPLPPGWEMTFTATGQRYFLNHIEKITTWQDPRKVMNQPLNHVNLHPSITSTSVPQRSMAVSQPNLAMNHQHQQVVATSLSPQNHPTQNQPTGLMSVPNALTTQQQQQQKLRLQRIQMERERIRMRQEELMRQEAALCRQLPMETETMAPVNTPAMSTDMRSVTNSSSDPFLNGGPYHSREQSTDSGLGLGCYSVPTTPEDFLSNMDEMDTGENSGQTPMTVNPQQTRFPDFLDCLPGTNVDLGTLESEDLIPLFNDVESALNKSEPFLTWL

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
58	Phosphorylation	SFFKEPDSGSHSRQS HHCCCCCCCCCCCCC	53.57	27087446
60	Phosphorylation	FKEPDSGSHSRQSST CCCCCCCCCCCCCCC	23.42	27087446
62	Phosphorylation	EPDSGSHSRQSSTDS CCCCCCCCCCCCCCC	33.44	23608596
65	Phosphorylation	SGSHSRQSSTDSSGG CCCCCCCCCCCCCCC	33.81	27087446
66	Phosphorylation	GSHSRQSSTDSSGGH CCCCCCCCCCCCCCC	28.09	30352176
67	Phosphorylation	SHSRQSSTDSSGGHP CCCCCCCCCCCCCCC	45.18	29514104
69	Phosphorylation	SRQSSTDSSGGHPGP CCCCCCCCCCCCCCC	30.85	27087446
70	Phosphorylation	RQSSTDSSGGHPGPR CCCCCCCCCCCCCCC	52.16	-
87	Phosphorylation	GGAQHVRSHSSPASL CCCCCCCCCCCCCCE	26.99	27087446
89	Phosphorylation	AQHVRSHSSPASLQL CCCCCCCCCCCCEEC	38.98	27087446
90	Phosphorylation	QHVRSHSSPASLQLG CCCCCCCCCCCEECC	21.04	27087446
93	Phosphorylation	RSHSSPASLQLGTGA CCCCCCCCEECCCCC	21.74	27742792
98	Phosphorylation	PASLQLGTGAGAAGG CCCEECCCCCCCCCC	32.24	25619855
117	Phosphorylation	HAHLRQQSYDVTDEL CHHHHHCCCCCCCCC	18.24	27087446
118	Phosphorylation	AHLRQQSYDVTDELP HHHHHCCCCCCCCCC	15.30	26643407
121	Phosphorylation	RQQSYDVTDELPLPP HHCCCCCCCCCCCCC	22.36	26643407
144	Phosphorylation	TGQRYFLNHIEKITT CCCEEEHHHHHHCCC	24.96	24719451
171	Phosphorylation	NHVNLHPSITSTSVP CCCCCCCCCCCCCCC	27.96	28059163
173	Phosphorylation	VNLHPSITSTSVPQR CCCCCCCCCCCCCCH	30.23	28059163
174	Phosphorylation	NLHPSITSTSVPQRS CCCCCCCCCCCCCHH	19.60	28059163
176	O-linked_Glycosylation	HPSITSTSVPQRSMA CCCCCCCCCCCHHHC	31.41	30059200
181	Phosphorylation	STSVPQRSMAVSQPN CCCCCCHHHCCCCCC	13.07	28059163
285	Phosphorylation	AMSTDMRSVTNSSSD CCCCCCHHHCCCCCC	27.01	23984901
287	Phosphorylation	STDMRSVTNSSSDPF CCCCHHHCCCCCCCC	30.54	23984901
289	Phosphorylation	DMRSVTNSSSDPFLN CCHHHCCCCCCCCCC	23.12	23984901
290	Phosphorylation	MRSVTNSSSDPFLNG CHHHCCCCCCCCCCC	40.61	23984901
291	Phosphorylation	RSVTNSSSDPFLNGG HHHCCCCCCCCCCCC	49.47	21183079
300	Phosphorylation	PFLNGGPYHSREQST CCCCCCCCCCCCCCC	18.72	23984901
302	Phosphorylation	LNGGPYHSREQSTDS CCCCCCCCCCCCCCC	31.50	25168779
306	Phosphorylation	PYHSREQSTDSGLGL CCCCCCCCCCCCCCC	29.45	-
309	Phosphorylation	SREQSTDSGLGLGCY CCCCCCCCCCCCCCE	35.69	21474562
316	Phosphorylation	SGLGLGCYSVPTTPE CCCCCCCEECCCCHH	15.76	-
347	Phosphorylation	QTPMTVNPQQTRFPD CCCCCCCCCCCCCCC	24.26	24719451

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
89	S	Phosphorylation	Kinase	LATS1	Q8BYR2	PSP
89	S	Phosphorylation	Kinase	LATS2	Q9NRM7	PSP
89	S	Phosphorylation	Kinase	LATS2	Q7TSJ6	Uniprot
306	S	Phosphorylation	Kinase	LATS2	Q7TSJ6	Uniprot
309	S	Phosphorylation	Kinase	NEK1	Q96PY6	PSP
316	Y	Phosphorylation	Kinase	ABL1	P00519	GPS
316	Y	Phosphorylation	Kinase	SRC	P12931	PSP

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of WWTR1_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of WWTR1_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
TLE6_MOUSE	Tle6	physical	20211142
NEK1_MOUSE	Nek1	physical	21474562
TTF1_MOUSE	Ttf1	physical	14970209
PAX3_MOUSE	Pax3	physical	16300735
SMAD2_MOUSE	Smad2	physical	21145499
MPP5_HUMAN	MPP5	physical	21145499
LIN7C_HUMAN	LIN7C	physical	21145499
INADL_HUMAN	INADL	physical	21145499
AMOT_HUMAN	AMOT	physical	21145499
LATS1_HUMAN	LATS1	physical	21145499
1433T_HUMAN	YWHAQ	physical	21145499
MPDZ_HUMAN	MPDZ	physical	21145499
MPP7_HUMAN	MPP7	physical	21145499
AMOL1_HUMAN	AMOTL1	physical	21145499
AMOL2_HUMAN	AMOTL2	physical	21145499
1433E_HUMAN	YWHAE	physical	21145499
1433B_HUMAN	YWHAB	physical	21145499
1433G_HUMAN	YWHAG	physical	21145499
1433Z_HUMAN	YWHAZ	physical	21145499
1433F_HUMAN	YWHAH	physical	21145499
MPP5_MOUSE	Mpp5	physical	21145499
LATS1_MOUSE	Lats1	physical	21145499
YAP1_MOUSE	Yap1	physical	21145499
1433E_MOUSE	Ywhae	physical	21145499
YAP1_MOUSE	Yap1	physical	16332960
WWTR1_MOUSE	Wwtr1	physical	16332960
PKD2_MOUSE	Pkd2	physical	17636028
FBW1A_MOUSE	Btrc	physical	17636028
CUL1_MOUSE	Cul1	physical	17636028
FBW1B_MOUSE	Fbxw11	physical	17636028

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of WWTR1_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Large-scale phosphorylation analysis of mouse liver."; Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-93, AND MASSSPECTROMETRY.	17242355 [Abstract]