PP4C_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: PP4C_MOUSE
• UniProt AC: P97470
• Protein Name: Serine/threonine-protein phosphatase 4 catalytic subunit
• Gene Name: Ppp4c
• Organism: Mus musculus (Mouse).
• Sequence Length: 307
• Subcellular Localization: Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
• Protein Description: Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA double strand break repair (By similarity). Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase. In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin (By similarity)..
• Protein Sequence: MAEISDLDRQIEQLRRCELIKESEVKALCAKAREILVEESNVQRVDSPVTVCGDIHGQFYDLKELFRVGGDVPETNYLFMGDFVDRGFYSVETFLLLLALKVRYPDRITLIRGNHESRQITQVYGFYDECLRKYGSVTVWRYCTEIFDYLSLSAIIDGKIFCVHGGLSPSIQTLDQIRTIDRKQEVPHDGPMCDLLWSDPEDTTGWGVSPRGAGYLFGSDVVAQFNAANDIDMICRAHQLVMEGYKWHFNETVLTVWSAPNYCYRCGNVAAILELDEHLQKDFIIFEAAPQETRGIPSKKPVADYFL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MAEISDLDR ------CCCHHHHHH	23.88	-
26	Ubiquitination	LIKESEVKALCAKAR HHCHHHHHHHHHHHH	31.43	-
31	Ubiquitination	EVKALCAKAREILVE HHHHHHHHHHHHHHC	47.16	-
104	Phosphorylation	LLALKVRYPDRITLI HHHHHCCCCCCEEEE	16.69	21454597
124	Phosphorylation	SRQITQVYGFYDECL HHHHHEEEECHHHHH	7.53	22817900
179	Phosphorylation	QTLDQIRTIDRKQEV HCHHHHCCCCCCCCC	28.94	-
293	Phosphorylation	FEAAPQETRGIPSKK EECCCCCCCCCCCCC	29.02	22817900
307	Methylation	KPVADYFL------- CCCHHHCC-------	5.81	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of PP4C_MOUSE !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
307	L	Methylation	Methylation at the C-terminal Leu-307 is critical for interactions with regulatory subunits and functions in DNA repair.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of PP4C_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
TCPZ_HUMAN	CCT6A	physical	26496610
CPT1A_HUMAN	CPT1A	physical	26496610
IGBP1_HUMAN	IGBP1	physical	26496610
MAGA1_HUMAN	MAGEA1	physical	26496610
NP1L1_HUMAN	NAP1L1	physical	26496610
2AAA_HUMAN	PPP2R1A	physical	26496610
RREB1_HUMAN	RREB1	physical	26496610
TCPA_HUMAN	TCP1	physical	26496610
TCPG_HUMAN	CCT3	physical	26496610
XPO1_HUMAN	XPO1	physical	26496610
CCDC6_HUMAN	CCDC6	physical	26496610
BAF_HUMAN	BANF1	physical	26496610
DNJA3_HUMAN	DNAJA3	physical	26496610
NPA1P_HUMAN	URB1	physical	26496610
PP4R1_HUMAN	PPP4R1	physical	26496610
TCPH_HUMAN	CCT7	physical	26496610
TCPD_HUMAN	CCT4	physical	26496610
TCPB_HUMAN	CCT2	physical	26496610
TCPQ_HUMAN	CCT8	physical	26496610
YMEL1_HUMAN	YME1L1	physical	26496610
MAGD2_HUMAN	MAGED2	physical	26496610
TCPE_HUMAN	CCT5	physical	26496610
NARF_HUMAN	NARF	physical	26496610
GAR1_HUMAN	GAR1	physical	26496610
NOP10_HUMAN	NOP10	physical	26496610
P4R3A_HUMAN	SMEK1	physical	26496610
P4R3B_HUMAN	SMEK2	physical	26496610
KLC2_HUMAN	KLC2	physical	26496610
CLP1L_HUMAN	CLPTM1L	physical	26496610
PP4R2_HUMAN	PPP4R2	physical	26496610

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.