dbPTM

KAR4_YEAST - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	KAR4_YEAST
UniProt AC	P25583
Protein Name	Karyogamy protein KAR4
Gene Name	KAR4
Organism	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
Sequence Length	335
Subcellular Localization	Nucleus.
Protein Description	May assist STE12 in the pheromone-dependent expression of KAR3 and CIK1. Also required for meiosis..
Protein Sequence	MAFQDPTYDQNKSRHINNSHLQGPNQETIEMKSKHVSFKPSRDFHTNDYSNNYIHGKSLPQQHVTNIENRVDGYPKLQKLFQAKAKQINQFATTPFGCKIGIDSIVPTLNHWIQNENLTFDVVMIGCLTENQFIYPILTQLPLDRLISKPGFLFIWANSQKINELTKLLNNEIWAKKFRRSEELVFVPIDKKSPFYPGLDQDDETLMEKMQWHCWMCITGTVRRSTDGHLIHCNVDTDLSIETKDTTNGAVPSHLYRIAENFSTATRRLHIIPARTGYETPVKVRPGWVIVSPDVMLDNFSPKRYKEEIANLGSNIPLKNEIELLRPRSPVQKAQ

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
46	Phosphorylation	KPSRDFHTNDYSNNY CCCCCCCCCCCCCCE	29.76	27017623
49	Phosphorylation	RDFHTNDYSNNYIHG CCCCCCCCCCCEECC	18.50	27017623
50	Phosphorylation	DFHTNDYSNNYIHGK CCCCCCCCCCEECCC	22.82	27017623
53	Phosphorylation	TNDYSNNYIHGKSLP CCCCCCCEECCCCCC	9.88	27017623
253	Phosphorylation	TTNGAVPSHLYRIAE CCCCCCCHHHHHHHH	21.37	19779198
256	Phosphorylation	GAVPSHLYRIAENFS CCCCHHHHHHHHHCC	8.44	19779198
264	Phosphorylation	RIAENFSTATRRLHI HHHHHCCHHHCEEEE	28.10	19779198
280	Phosphorylation	PARTGYETPVKVRPG ECCCCCCCCCEEECC	25.54	27214570
292	Phosphorylation	RPGWVIVSPDVMLDN ECCEEEECCCEECCC	12.31	28889911
301	Phosphorylation	DVMLDNFSPKRYKEE CEECCCCCHHHHHHH	35.45	21440633
329	Phosphorylation	IELLRPRSPVQKAQ- HHHHCCCCCCCCCC-	31.91	25521595

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of KAR4_YEAST !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of KAR4_YEAST !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of KAR4_YEAST !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
MUM2_YEAST	MUM2	physical	10688190
STE12_YEAST	STE12	genetic	8754797
YG036_YEAST	YGL036W	physical	11283351
IME4_YEAST	IME4	physical	11283351
CAF40_YEAST	CAF40	physical	18467557
NUP84_YEAST	NUP84	genetic	19269370
PTPA1_YEAST	RRD1	genetic	19269370
SA190_YEAST	SAP190	genetic	19269370
BUB1_YEAST	BUB1	genetic	20959818
RRP6_YEAST	RRP6	genetic	20959818
HAP4_YEAST	HAP4	genetic	20959818
RTG3_YEAST	RTG3	genetic	21127252
ATM_YEAST	TEL1	genetic	21127252
CAF40_YEAST	CAF40	physical	22615397
CALM_YEAST	CMD1	genetic	27708008
MCM7_YEAST	MCM7	genetic	27708008
KIN28_YEAST	KIN28	genetic	27708008
RRP1_YEAST	RRP1	genetic	27708008
NSE3_YEAST	NSE3	genetic	27708008
ACT_YEAST	ACT1	genetic	27708008
MCE1_YEAST	CEG1	genetic	27708008
MED6_YEAST	MED6	genetic	27708008
ATC7_YEAST	NEO1	genetic	27708008
YKT6_YEAST	YKT6	genetic	27708008
BET3_YEAST	BET3	genetic	27708008
SSL1_YEAST	SSL1	genetic	27708008
NMT_YEAST	NMT1	genetic	27708008
POB3_YEAST	POB3	genetic	27708008
SEC65_YEAST	SEC65	genetic	27708008
MCM1_YEAST	MCM1	genetic	27708008
RNA1_YEAST	RNA1	genetic	27708008
RPC1_YEAST	RPO31	genetic	27708008
CLP1_YEAST	CLP1	genetic	27708008
CET1_YEAST	CET1	genetic	27708008
SEC23_YEAST	SEC23	genetic	27708008

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of KAR4_YEAST

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"A multidimensional chromatography technology for in-depthphosphoproteome analysis."; Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.; Mol. Cell. Proteomics 7:1389-1396(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-292; SER-301 ANDSER-329, AND MASS SPECTROMETRY.	18407956 [Abstract]