dbPTM

DCX_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	DCX_HUMAN	Jmol._Canvas2D (Jmol) "myJmol"[x]
UniProt AC	O43602
Protein Name	Neuronal migration protein doublecortin
Gene Name	DCX
Organism	Homo sapiens (Human).
Sequence Length	365
Subcellular Localization	Cytoplasm . Cell projection . Localizes at neurite tips.
Protein Description	Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with PAFAH1B1/LIS-1 of overlapping, but distinct, signaling pathways that promote neuronal migration..
Protein Sequence	MELDFGHFDERDKTSRNMRGSRMNGLPSPTHSAHCSFYRTRTLQALSNEKKAKKVRFYRNGDRYFKGIVYAVSSDRFRSFDALLADLTRSLSDNINLPQGVRYIYTIDGSRKIGSMDELEEGESYVCSSDNFFKKVEYTKNVNPNWSVNVKTSANMKAPQSLASSNSAQARENKDFVRPKLVTIIRSGVKPRKAVRVLLNKKTAHSFEQVLTDITEAIKLETGVVKKLYTLDGKQVTCLHDFFGDDDVFIACGPEKFRYAQDDFSLDENECRVMKGNPSATAGPKASPTPQKTSAKSPGPMRRSKSPADSGNDQDANGTSSSQLSTPKSKQSPISTPTSPGSLRKHKDLYLPLSLDDSDSLGDSM

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
14	Phosphorylation	HFDERDKTSRNMRGS CCCCCCCCCCCCCCC	27.00	29691806
15	Phosphorylation	FDERDKTSRNMRGSR CCCCCCCCCCCCCCC	36.56	29691806
21	Phosphorylation	TSRNMRGSRMNGLPS CCCCCCCCCCCCCCC	37.97	29691806
28	Phosphorylation	SRMNGLPSPTHSAHC CCCCCCCCCCCHHCC	28.59	27732954
30	Phosphorylation	MNGLPSPTHSAHCSF CCCCCCCCCHHCCHH	4.25	27732954
32	Phosphorylation	GLPSPTHSAHCSFYR CCCCCCCHHCCHHHH	8.79	24719451
36	Phosphorylation	PTHSAHCSFYRTRTL CCCHHCCHHHHHHHH	4.84	22210691
38	Phosphorylation	HSAHCSFYRTRTLQA CHHCCHHHHHHHHHH	45.07	27732954
42	Phosphorylation	CSFYRTRTLQALSNE CHHHHHHHHHHHCCC	16.06	22985185
47	Phosphorylation	TRTLQALSNEKKAKK HHHHHHHCCCCHHCE	15.46	15632197
50	Acetylation	LQALSNEKKAKKVRF HHHHCCCCHHCEEEE	15.03	20167786
51	Acetylation	QALSNEKKAKKVRFY HHHCCCCHHCEEEEE	2.24	20167786
64	Phosphorylation	FYRNGDRYFKGIVYA EEECCCCCCEEEEEE	29.98	-
70	Phosphorylation	RYFKGIVYAVSSDRF CCCEEEEEEECCCHH	29.59	14757045
74	Phosphorylation	GIVYAVSSDRFRSFD EEEEEECCCHHHCHH	39.82	-
79	Phosphorylation	VSSDRFRSFDALLAD ECCCHHHCHHHHHHH	16.21	22210691
89	Phosphorylation	ALLADLTRSLSDNIN HHHHHHHHHHHCCCC	12.19	15632197
90	Phosphorylation	LLADLTRSLSDNINL HHHHHHHHHHCCCCC	44.33	21082442
103	Phosphorylation	NLPQGVRYIYTIDGS CCCCCCEEEEEEECC	25.96	-
105	Phosphorylation	PQGVRYIYTIDGSRK CCCCEEEEEEECCCE	47.98	-
109	Phosphorylation	RYIYTIDGSRKIGSM EEEEEEECCCEECCC	47.69	14741103
110	Phosphorylation	YIYTIDGSRKIGSMD EEEEEECCCEECCCC	31.99	-
111	Phosphorylation	IYTIDGSRKIGSMDE EEEEECCCEECCCCH	32.57	-
112	Phosphorylation	YTIDGSRKIGSMDEL EEEECCCEECCCCHH	26.77	14757045
113	Phosphorylation	TIDGSRKIGSMDELE EEECCCEECCCCHHH	23.37	24719451
115	Phosphorylation	DGSRKIGSMDELEEG ECCCEECCCCHHHCC	15.69	-
128	Phosphorylation	EGESYVCSSDNFFKK CCCEEEECCCCCCCE	46.06	15632197
131	Acetylation	SYVCSSDNFFKKVEY EEEECCCCCCCEEEE	63.38	-
132	Acetylation	YVCSSDNFFKKVEYT EEECCCCCCCEEEEE	60.30	-
147	Phosphorylation	KNVNPNWSVNVKTSA CCCCCCCEEEEECCC	38.50	28857561
151	Phosphorylation	PNWSVNVKTSANMKA CCCEEEEECCCCCCC	10.20	14757045
153	Phosphorylation	WSVNVKTSANMKAPQ CEEEEECCCCCCCCH	2.85	27067055
155	Phosphorylation	VNVKTSANMKAPQSL EEEECCCCCCCCHHH	26.63	-
161	Phosphorylation	ANMKAPQSLASSNSA CCCCCCHHHHCCCHH	5.77	29083192
164	Phosphorylation	KAPQSLASSNSAQAR CCCHHHHCCCHHHHH	4.33	29083192
165	Phosphorylation	APQSLASSNSAQARE CCHHHHCCCHHHHHH	3.60	29083192
167	Phosphorylation	QSLASSNSAQARENK HHHHCCCHHHHHHCC	48.84	29083192
171	Phosphorylation	SSNSAQARENKDFVR CCCHHHHHHCCCCCC	27.30	19060867
184	Phosphorylation	VRPKLVTIIRSGVKP CCHHHHHHHHCCCCC	8.89	-
186	Phosphorylation	PKLVTIIRSGVKPRK HHHHHHHHCCCCCHH	6.68	-
203	Phosphorylation	RVLLNKKTAHSFEQV HHHCCCCCCCCHHHH	21.56	27732954
206	Phosphorylation	LNKKTAHSFEQVLTD CCCCCCCCHHHHHHH	10.94	27732954
215	Phosphorylation	EQVLTDITEAIKLET HHHHHHHHHHHHHHC	52.34	22210691
222	Phosphorylation	TEAIKLETGVVKKLY HHHHHHHCCCEEEEE	30.88	-
230	Phosphorylation	GVVKKLYTLDGKQVT CCEEEEEECCCCEEE	24.85	27135362
265	Phosphorylation	RYAQDDFSLDENECR CCCCCCCCCCCCCCE	1.60	27732954
281	Phosphorylation	MKGNPSATAGPKASP ECCCCCCCCCCCCCC	39.35	28348404
284	Phosphorylation	NPSATAGPKASPTPQ CCCCCCCCCCCCCCC	31.08	-
287	Phosphorylation	ATAGPKASPTPQKTS CCCCCCCCCCCCCCC	28.19	14741103
289	Phosphorylation	AGPKASPTPQKTSAK CCCCCCCCCCCCCCC	42.68	14741103
294	Phosphorylation	SPTPQKTSAKSPGPM CCCCCCCCCCCCCCC	41.47	-
297	Phosphorylation	PQKTSAKSPGPMRRS CCCCCCCCCCCCCCC	49.05	14741103
303	Phosphorylation	KSPGPMRRSKSPADS CCCCCCCCCCCCCCC	45.79	-
304	Phosphorylation	SPGPMRRSKSPADSG CCCCCCCCCCCCCCC	16.91	25921289
304 (in isoform 2)	Phosphorylation	-	16.91	25954137
306	Phosphorylation	GPMRRSKSPADSGND CCCCCCCCCCCCCCC	4.84	15345747
306 (in isoform 2)	Phosphorylation	-	4.84	15345747
310	Phosphorylation	RSKSPADSGNDQDAN CCCCCCCCCCCCCCC	17.71	25921289
310 (in isoform 2)	Phosphorylation	-	17.71	25954137
315 (in isoform 2)	Phosphorylation	-	41.06	15345747
319	Phosphorylation	NDQDANGTSSSQLST CCCCCCCCCHHHCCC	3.14	30177828
320	Phosphorylation	DQDANGTSSSQLSTP CCCCCCCCHHHCCCC	2.95	15345747
320 (in isoform 2)	Phosphorylation	-	2.95	25954137
321	Phosphorylation	QDANGTSSSQLSTPK CCCCCCCHHHCCCCC	31.79	30177828
322	Phosphorylation	DANGTSSSQLSTPKS CCCCCCHHHCCCCCC	27.85	30177828
324 (in isoform 2)	Phosphorylation	-	15.16	25954137
325	Phosphorylation	GTSSSQLSTPKSKQS CCCHHHCCCCCCCCC	37.11	30177828
326	Phosphorylation	TSSSQLSTPKSKQSP CCHHHCCCCCCCCCC	43.72	14741103
329	Phosphorylation	SQLSTPKSKQSPIST HHCCCCCCCCCCCCC	4.87	20068231
332	Phosphorylation	STPKSKQSPISTPTS CCCCCCCCCCCCCCC	4.83	24117733
335	Phosphorylation	KSKQSPISTPTSPGS CCCCCCCCCCCCCCC	41.30	24117733
336	Phosphorylation	SKQSPISTPTSPGSL CCCCCCCCCCCCCCC	50.54	24117733
338	Phosphorylation	QSPISTPTSPGSLRK CCCCCCCCCCCCCCC	7.42	24117733
339	Phosphorylation	SPISTPTSPGSLRKH CCCCCCCCCCCCCCC	36.62	29900121
342	Phosphorylation	STPTSPGSLRKHKDL CCCCCCCCCCCCCCE	41.70	24117733
345	Acetylation	TSPGSLRKHKDLYLP CCCCCCCCCCCEEEC	11.98	7706845
346	Phosphorylation	SPGSLRKHKDLYLPL CCCCCCCCCCEEECC	41.74	19060867
347	Acetylation	PGSLRKHKDLYLPLS CCCCCCCCCEEECCC	10.92	7706859
350	Phosphorylation	LRKHKDLYLPLSLDD CCCCCCEEECCCCCC	54.02	30177828
354	Phosphorylation	KDLYLPLSLDDSDSL CCEEECCCCCCCCCC	7.60	27732954
358	Phosphorylation	LPLSLDDSDSLGDSM ECCCCCCCCCCCCCC	23.10	27732954
360	Phosphorylation	LSLDDSDSLGDSM-- CCCCCCCCCCCCC--	42.10	27732954
364	Phosphorylation	DSDSLGDSM------ CCCCCCCCC------	21.64	27732954
368	Phosphorylation	LGDSM---------- CCCCC----------	35.12	14741103
370	Phosphorylation	DSM------------ CCC------------	35.54	14741103
372	Phosphorylation	M-------------- C--------------	65.23	17081983
378	Phosphorylation	-------------------- --------------------	34.50	14741103
387	Phosphorylation	----------------------------- -----------------------------	26.94	15345747
396	Phosphorylation	-------------------------------------- --------------------------------------	29.41	15345747
401	Phosphorylation	------------------------------------------- -------------------------------------------	34.72	-
402	Phosphorylation	-------------------------------------------- --------------------------------------------	43.24	14741103
405	Phosphorylation	----------------------------------------------- -----------------------------------------------	36.81	20068231
408	Phosphorylation	-------------------------------------------------- --------------------------------------------------	28.12	14741103
411	Phosphorylation	----------------------------------------------------- -----------------------------------------------------	31.55	22817900
412	Phosphorylation	------------------------------------------------------ ------------------------------------------------------	31.16	14741103
415	Phosphorylation	--------------------------------------------------------- ---------------------------------------------------------	28.59	24117733

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
14	T	Phosphorylation	Kinase	PKC	-	Uniprot
28	S	Phosphorylation	Kinase	CDK5	Q00535	Uniprot
47	S	Phosphorylation	Kinase	PKA	-	Uniprot
47	S	Phosphorylation	Kinase	MARK1	Q9P0L2	Uniprot
70	Y	Phosphorylation	Kinase	ABL	P00519	Uniprot
74	S	Phosphorylation	Kinase	PKC	-	Uniprot
90	S	Phosphorylation	Kinase	CK2	-	Uniprot
110	S	Phosphorylation	Kinase	PKC	-	Uniprot
115	S	Phosphorylation	Kinase	MARK1	Q9P0L2	Uniprot
115	S	Phosphorylation	Kinase	PKA	-	Uniprot
115	S	Phosphorylation	Kinase	CK2	-	Uniprot
265	S	Phosphorylation	Kinase	CK2	-	Uniprot
287	S	Phosphorylation	Kinase	CDK5	Q00535	Uniprot
289	T	Phosphorylation	Kinase	CDK5	Q00535	Uniprot
294	S	Phosphorylation	Kinase	PKC	-	Uniprot
297	S	Phosphorylation	Kinase	CDK5	Q00535	Uniprot
306	S	Phosphorylation	Kinase	DYRK2	Q92630	PSP
306	S	Phosphorylation	Kinase	DYRK2	Q92630	Uniprot
321	T	Phosphorylation	Kinase	MAPK8	P49185	GPS
321	T	Phosphorylation	Kinase	JNK2	P45984	PSP
321	T	Phosphorylation	Kinase	MAPK9	P49186	GPS
326	T	Phosphorylation	Kinase	PKC	-	Uniprot
326	T	Phosphorylation	Kinase	MAPK	-	Uniprot
327	S	Phosphorylation	Kinase	GSK3B	P49841	PSP
331	T	Phosphorylation	Kinase	MAPK9	P49186	GPS
331	T	Phosphorylation	Kinase	JNK2	P45984	PSP
331	T	Phosphorylation	Kinase	MAPK8	P49185	GPS
332	S	Phosphorylation	Kinase	MAPK	-	Uniprot
334	S	Phosphorylation	Kinase	MAPK9	P49186	GPS
334	S	Phosphorylation	Kinase	JNK2	P45984	PSP
334	S	Phosphorylation	Kinase	MAPK8	P49185	GPS
336	T	Phosphorylation	Kinase	MAPK	-	Uniprot
339	S	Phosphorylation	Kinase	MAPK	-	Uniprot
342	S	Phosphorylation	Kinase	PKC	-	Uniprot
354	S	Phosphorylation	Kinase	CK2	-	Uniprot
360	S	Phosphorylation	Kinase	CK2	-	Uniprot
-	K	Ubiquitination	E3 ubiquitin ligase	MDM2	Q00987	PMID:22199232

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Reference
265	S	Phosphorylation	-
Phosphorylation at Ser-265 and Ser-297 seems to occur only in neonatal brain, the levels falling precipitously by postnatal day 21 (By similarity).
297	S	Phosphorylation	-
Phosphorylation at Ser-265 and Ser-297 seems to occur only in neonatal brain, the levels falling precipitously by postnatal day 21 (By similarity).

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of DCX_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
LIS1_HUMAN	PAFAH1B1	physical	11001923
DCX_HUMAN	DCX	physical	11001923
USP9X_HUMAN	USP9X	physical	15607950
PP1A_HUMAN	PPP1CA	physical	19094064
A4_HUMAN	APP	physical	21832049
GOGA2_HUMAN	GOLGA2	physical	25416956
KIFC3_HUMAN	KIFC3	physical	25416956
MEOX1_HUMAN	MEOX1	physical	25416956
TRI27_HUMAN	TRIM27	physical	25416956
ZBTB5_HUMAN	ZBTB5	physical	25416956
CACO2_HUMAN	CALCOCO2	physical	25416956
IKZF1_HUMAN	IKZF1	physical	25416956
SPAG5_HUMAN	SPAG5	physical	25416956
TRIM1_HUMAN	MID2	physical	25416956
RINT1_HUMAN	RINT1	physical	25416956
K1C40_HUMAN	KRT40	physical	25416956
KR108_HUMAN	KRTAP10-8	physical	25416956
DCLK1_HUMAN	DCLK1	physical	26186194
NMNA3_HUMAN	NMNAT3	physical	26186194
RRP44_HUMAN	DIS3	physical	26186194
CUL1_HUMAN	CUL1	physical	26186194
GOGA2_HUMAN	GOLGA2	physical	21516116
NEB2_HUMAN	PPP1R9B	physical	17178868
NMNA3_HUMAN	NMNAT3	physical	28514442
DCLK1_HUMAN	DCLK1	physical	28514442
RRP44_HUMAN	DIS3	physical	28514442
CUL1_HUMAN	CUL1	physical	28514442

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
300067	Lissencephaly, X-linked 1 (LISX1)
300067	Subcortical band heterotopia X-linked (SBHX)
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of DCX_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Global, in vivo, and site-specific phosphorylation dynamics insignaling networks."; Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P.,Mann M.; Cell 127:635-648(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-411, AND MASSSPECTROMETRY.	17081983 [Abstract]

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