SPY1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: SPY1_HUMAN
• UniProt AC: O43609
• Protein Name: Protein sprouty homolog 1
• Gene Name: SPRY1
• Organism: Homo sapiens (Human).
• Sequence Length: 319
• Subcellular Localization: Cytoplasm. Membrane; Peripheral membrane protein. Found in the cytoplasm in unstimulated cells but is translocated to the membrane ruffles in cells stimulated with EGF (epidermal growth factor).
• Protein Description: May function as an antagonist of fibroblast growth factor (FGF) pathways and may negatively modulate respiratory organogenesis..
• Protein Sequence: MDPQNQHGSGSSLVVIQQPSLDSRQRLDYEREIQPTAILSLDQIKAIRGSNEYTEGPSVVKRPAPRTAPRQEKHERTHEIIPINVNNNYEHRHTSHLGHAVLPSNARGPILSRSTSTGSAASSGSNSSASSEQGLLGRSPPTRPVPGHRSERAIRTQPKQLIVDDLKGSLKEDLTQHKFICEQCGKCKCGECTAPRTLPSCLACNRQCLCSAESMVEYGTCMCLVKGIFYHCSNDDEGDSYSDNPCSCSQSHCCSRYLCMGAMSLFLPCLLCYPPAKGCLKLCRRCYDWIHRPGCRCKNSNTVYCKLESCPSRGQGKPS

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MDPQNQHG -------CCCCCCCC	17.89	22223895
9	Phosphorylation	DPQNQHGSGSSLVVI CCCCCCCCCCCEEEE	33.12	25850435
11	Phosphorylation	QNQHGSGSSLVVIQQ CCCCCCCCCEEEEEC	23.38	27251275
12	Phosphorylation	NQHGSGSSLVVIQQP CCCCCCCCEEEEECC	28.80	27251275
36	Phosphorylation	YEREIQPTAILSLDQ CCCCCCCCEEEEHHH	15.23	25693802
40	Phosphorylation	IQPTAILSLDQIKAI CCCCEEEEHHHHHHH	24.64	25693802
45	Ubiquitination	ILSLDQIKAIRGSNE EEEHHHHHHHCCCCC	31.93	32015554
50	Phosphorylation	QIKAIRGSNEYTEGP HHHHHCCCCCCCCCC	19.35	25850435
53	Phosphorylation	AIRGSNEYTEGPSVV HHCCCCCCCCCCCCC	17.84	25159151
54	Phosphorylation	IRGSNEYTEGPSVVK HCCCCCCCCCCCCCC	28.43	26356563
89	Phosphorylation	PINVNNNYEHRHTSH EECCCCCCCCCCCCC	18.42	29978859
95	Phosphorylation	NYEHRHTSHLGHAVL CCCCCCCCCCCEEEC	15.58	26670566
112	Phosphorylation	NARGPILSRSTSTGS CCCCCCEECCCCCCC	25.55	24719451
114	Phosphorylation	RGPILSRSTSTGSAA CCCCEECCCCCCCCC	24.27	28348404
115	Phosphorylation	GPILSRSTSTGSAAS CCCEECCCCCCCCCC	29.24	28348404
116	Phosphorylation	PILSRSTSTGSAASS CCEECCCCCCCCCCC	31.83	28348404
117	Phosphorylation	ILSRSTSTGSAASSG CEECCCCCCCCCCCC	34.93	28348404
119	Phosphorylation	SRSTSTGSAASSGSN ECCCCCCCCCCCCCC	22.28	28348404
122	Phosphorylation	TSTGSAASSGSNSSA CCCCCCCCCCCCCCC	34.64	22964224
123	Phosphorylation	STGSAASSGSNSSAS CCCCCCCCCCCCCCC	41.33	22964224
125	Phosphorylation	GSAASSGSNSSASSE CCCCCCCCCCCCCCC	35.17	22964224
127	Phosphorylation	AASSGSNSSASSEQG CCCCCCCCCCCCCCC	29.04	30377224
128	Phosphorylation	ASSGSNSSASSEQGL CCCCCCCCCCCCCCC	36.10	30377224
131	Phosphorylation	GSNSSASSEQGLLGR CCCCCCCCCCCCCCC	33.39	-
139	Phosphorylation	EQGLLGRSPPTRPVP CCCCCCCCCCCCCCC	33.17	29255136
167	Ubiquitination	QLIVDDLKGSLKEDL EEEHHCCCCCCCHHH	54.14	32015554
169	Phosphorylation	IVDDLKGSLKEDLTQ EHHCCCCCCCHHHHH	34.38	24501219
171	Ubiquitination	DDLKGSLKEDLTQHK HCCCCCCCHHHHHCC	51.37	29967540
304	Phosphorylation	CKNSNTVYCKLESCP CCCCCCEEEEEECCC	5.05	-
306	Ubiquitination	NSNTVYCKLESCPSR CCCCEEEEEECCCCC	37.29	32015554

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of SPY1_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of SPY1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of SPY1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
TESK1_HUMAN	TESK1	physical	17974561
CBL_HUMAN	CBL	physical	15004239
CBLB_HUMAN	CBLB	physical	19915061
CBL_HUMAN	CBL	physical	19915061
LAT_HUMAN	LAT	physical	19915061
GRB2_HUMAN	GRB2	physical	16893902
CBL_HUMAN	CBL	physical	16893902
STAT3_HUMAN	STAT3	physical	21988832
TYY1_HUMAN	YY1	physical	21988832
SPY2_HUMAN	SPRY2	physical	25416956
R3HD2_HUMAN	R3HDM2	physical	25416956
KRA42_HUMAN	KRTAP4-2	physical	25416956
HEXI2_HUMAN	HEXIM2	physical	25416956
LCE1B_HUMAN	LCE1B	physical	25416956
LCE2D_HUMAN	LCE2D	physical	25416956
KR107_HUMAN	KRTAP10-7	physical	25416956
KR109_HUMAN	KRTAP10-9	physical	25416956
KR101_HUMAN	KRTAP10-1	physical	25416956
KR108_HUMAN	KRTAP10-8	physical	25416956
KR103_HUMAN	KRTAP10-3	physical	25416956
NT2NL_HUMAN	NOTCH2NL	physical	25416956
HXA1_HUMAN	HOXA1	physical	21516116

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"An extensive survey of tyrosine phosphorylation revealing new sitesin human mammary epithelial cells.";
Heibeck T.H., Ding S.-J., Opresko L.K., Zhao R., Schepmoes A.A.,Yang F., Tolmachev A.V., Monroe M.E., Camp D.G. II, Smith R.D.,Wiley H.S., Qian W.-J.;
J. Proteome Res. 8:3852-3861(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-53, AND MASSSPECTROMETRY.
PubMed: 19534553

"Multiple reaction monitoring for robust quantitative proteomicanalysis of cellular signaling networks.";
Wolf-Yadlin A., Hautaniemi S., Lauffenburger D.A., White F.M.;
Proc. Natl. Acad. Sci. U.S.A. 104:5860-5865(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-53, AND MASSSPECTROMETRY.
PubMed: 17389395

"Global survey of phosphotyrosine signaling identifies oncogenickinases in lung cancer.";
Rikova K., Guo A., Zeng Q., Possemato A., Yu J., Haack H., Nardone J.,Lee K., Reeves C., Li Y., Hu Y., Tan Z., Stokes M., Sullivan L.,Mitchell J., Wetzel R., Macneill J., Ren J.M., Yuan J.,Bakalarski C.E., Villen J., Kornhauser J.M., Smith B., Li D., Zhou X.,Gygi S.P., Gu T.-L., Polakiewicz R.D., Rush J., Comb M.J.;
Cell 131:1190-1203(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-53, AND MASSSPECTROMETRY.
PubMed: 18083107