| UniProt ID | SMUF2_MOUSE | |
|---|---|---|
| UniProt AC | A2A5Z6 | |
| Protein Name | E3 ubiquitin-protein ligase SMURF2 | |
| Gene Name | Smurf2 | |
| Organism | Mus musculus (Mouse). | |
| Sequence Length | 748 | |
| Subcellular Localization | Nucleus . Cytoplasm . Cell membrane . Membrane raft . Cytoplasmic in the presence of SMAD7. Colocalizes with CAV1, SMAD7 and TGF-beta receptor in membrane rafts. Interacts with STAMBP and RNF11.. | |
| Protein Description | E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.. | |
| Protein Sequence | MSNPGGRRNGPVKLRLTVLCAKNLVKKDFFRLPDPFAKVVVDGSGQCHSTDTVKNTLDPKWNQHYDLYIGKSDSVTISVWNHKKIHKKQGAGFLGCVRLLSNAINRLKDTGYQRLDLCKLGPNDNDTVRGQIVVSLQSRDRIGTGGQVVDCSRLFDNDLPDGWEERRTASGRIQYLNHITRTTQWERPTRPASEYSSPGRPLSCFVDENTPITGTNGATCGHSSDPRLAERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNINCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRLSANLHLVLNRQNQLKDQQQQQVVPLCPDDTECLTVPRYKRDLVQKLKILRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKSITLDDMELVDPDLHNSLVWILENDITGVLDHTFCVEHNAYGEIIQHELKPNGKSIPVTEENKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKTFDEKELELIICGLGKIDVSDWKVNTRLKHCTPDSNVVKWFWKAVEFFDEERRARLLQFVTGSSRVPLQGFKALQGAAGPRLFTIHQIDACTNNLPKAHTCFNRIDIPPYESYEKLYEKLLTAIEETCGFAVE | |
| Overview of Protein Modification Sites with Functional and Structural Information | ||
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* ASA = Accessible Surface Area
| Locations | Modification | Substrate Peptides & Secondary Structure |
ASA (%) | Reference | Orthologous Protein Cluster |
|---|---|---|---|---|---|
| 44 | Phosphorylation | AKVVVDGSGQCHSTD CEEEECCCCCCCCCC | 22.64 | - | |
| 268 | Phosphorylation | TTQQGQVYFLHTQTG ECCCCEEEEEEECCC | 7.79 | 29514104 | |
| 323 | Phosphorylation | VDHNNRTTQFTDPRL EECCCCEEEECCCHH | 20.60 | - | |
| 345 | Ubiquitination | LNRQNQLKDQQQQQV HCCHHHCCHHHHHCC | 43.67 | 22790023 | |
| 406 | Phosphorylation | REEIFEESYRQVMKM HHHHHHHHHHHHHHC | 20.58 | 28059163 | |
| 407 | Phosphorylation | EEIFEESYRQVMKMR HHHHHHHHHHHHHCC | 14.35 | 28059163 | |
| 615 | Ubiquitination | VIPQHLLKTFDEKEL HCCHHHHHCCCHHHH | 54.21 | 22790023 |
| Modified Location | Modified Residue | Modification | Type of Upstream Proteins | Gene Name of Upstream Proteins | UniProt AC of Upstream Proteins | Sources |
|---|---|---|---|---|---|---|
Oops, there are no upstream regulatory protein records of SMUF2_MOUSE !! | ||||||
| Modified Location | Modified Residue | Modification | Function | Reference | ||
|---|---|---|---|---|---|---|
Oops, there are no descriptions of PTM sites of SMUF2_MOUSE !! | ||||||
* Distance = the distance between SAP position and PTM sites.
| Modified Location | Modification | Variant Position (Distance <= 10) |
Residue Change | SAP | Related Disease | Reference |
|---|---|---|---|---|---|---|
Oops, there are no SNP-PTM records of SMUF2_MOUSE !! | ||||||
| Interacting Protein | Gene Name | Interaction Type | PPI Reference | Domain-Domain Interactions |
|---|---|---|---|---|
| SKIL_MOUSE | Skil | physical | 16959829 | |
| ANKR6_HUMAN | ANKRD6 | physical | 19379695 | |
| DVL2_HUMAN | DVL2 | physical | 19379695 | |
| DVL3_HUMAN | DVL3 | physical | 19379695 | |
| ELAV1_HUMAN | ELAVL1 | physical | 19379695 | |
| PPM1B_HUMAN | PPM1B | physical | 19379695 | |
| PTEN_HUMAN | PTEN | physical | 19379695 | |
| TP53B_MOUSE | Trp53bp1 | physical | 19379695 | |
| RAC1_MOUSE | Rac1 | physical | 19379695 | |
| NKD2_MOUSE | Nkd2 | physical | 19379695 | |
| DVL1_MOUSE | Dvl1 | physical | 19379695 | |
| DVL2_MOUSE | Dvl2 | physical | 19379695 | |
| PAR6A_MOUSE | Pard6a | physical | 19379695 | |
| SMAD1_MOUSE | Smad1 | physical | 23969857 | |
| RUNX2_MOUSE | Runx2 | physical | 23969857 | |
| SMAD7_MOUSE | Smad7 | physical | 25885904 | |
| TGFR1_MOUSE | Tgfbr1 | physical | 25885904 | |
| CLIP1_HUMAN | CLIP1 | physical | 26496610 | |
| RSRC1_HUMAN | RSRC1 | physical | 26496610 | |
| UBP35_HUMAN | USP35 | physical | 26496610 | |
| SMAD7_MOUSE | Smad7 | physical | 25612247 |
| Kegg Drug | ||||||
|---|---|---|---|---|---|---|
| DrugBank | ||||||
| There are no disease associations of PTM sites. | ||||||
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