TGFR1_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: TGFR1_MOUSE
• UniProt AC: Q64729
• Protein Name: TGF-beta receptor type-1
• Gene Name: Tgfbr1
• Organism: Mus musculus (Mouse).
• Sequence Length: 503
• Subcellular Localization: Cell membrane ; Single-pass type I membrane protein . Cell junction, tight junction . Membrane raft . Cell surface .
• Protein Description: Transmembrane serine/threonine kinase forming with the TGF-beta type II serine/threonine kinase receptor, TGFBR2, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and the activation of TGFBR1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade. Also involved in non-canonical, SMAD-independent TGF-beta signaling pathways. For instance, TGFBR1 induces TRAF6 autoubiquitination which in turn results in MAP3K7 ubiquitination and activation to trigger apoptosis. Also regulates epithelial to mesenchymal transition through a SMAD-independent signaling pathway through PARD6A phosphorylation and activation (By similarity)..
• Protein Sequence: MEAAAAAPRRPQLLIVLVAAATLLPGAKALQCFCHLCTKDNFTCETDGLCFVSVTETTDKVIHNSMCIAEIDLIPRDRPFVCAPSSKTGAVTTTYCCNQDHCNKIELPTTGPFSEKQSAGLGPVELAAVIAGPVCFVCIALMLMVYICHNRTVIHHRVPNEEDPSLDRPFISEGTTLKDLIYDMTTSGSGSGLPLLVQRTIARTIVLQESIGKGRFGEVWRGKWRGEEVAVKIFSSREERSWFREAEIYQTVMLRHENILGFIAADNKDNGTWTQLWLVSDYHEHGSLFDYLNRYTVTVEGMIKLALSTASGLAHLHMEIVGTQGKPAIAHRDLKSKNILVKKNGTCCIADLGLAVRHDSATDTIDIAPNHRVGTKRYMAPEVLDDSINMKHFESFKRADIYAMGLVFWEIARRCSIGGIHEDYQLPYYDLVPSDPSVEEMRKVVCEQKLRPNIPNRWQSCEALRVMAKIMRECWYANGAARLTALRIKKTLSQLSQQEGIKM

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
41	N-linked_Glycosylation	CHLCTKDNFTCETDG HHHHCCCCCEECCCC	35.37	-
43	Phosphorylation	LCTKDNFTCETDGLC HHCCCCCEECCCCEE	18.71	-
165	Phosphorylation	VPNEEDPSLDRPFIS CCCCCCCCCCCCCCC	56.05	27180971
172	Phosphorylation	SLDRPFISEGTTLKD CCCCCCCCCCCCHHH	30.28	28066266
175	Phosphorylation	RPFISEGTTLKDLIY CCCCCCCCCHHHHHH	25.55	28066266
182	Phosphorylation	TTLKDLIYDMTTSGS CCHHHHHHHHHCCCC	13.63	-
185	Phosphorylation	KDLIYDMTTSGSGSG HHHHHHHHCCCCCCC	18.21	8947046
186	Phosphorylation	DLIYDMTTSGSGSGL HHHHHHHCCCCCCCH	25.18	8947046
187	Phosphorylation	LIYDMTTSGSGSGLP HHHHHHCCCCCCCHH	23.07	8947046
189	Phosphorylation	YDMTTSGSGSGLPLL HHHHCCCCCCCHHHH	29.56	8947046
191	Phosphorylation	MTTSGSGSGLPLLVQ HHCCCCCCCHHHHHH	38.56	8947046

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
165	S	Phosphorylation	Kinase	TGFBR2	Q62312	PSP
185	T	Phosphorylation	Kinase	TGFBR2	Q62312	Uniprot
186	T	Phosphorylation	Kinase	TGFBR2	Q62312	Uniprot
187	S	Phosphorylation	Kinase	TGFBR2	Q62312	Uniprot
189	S	Phosphorylation	Kinase	TGFBR2	Q62312	Uniprot
191	S	Phosphorylation	Kinase	TGFBR2	Q62312	Uniprot

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of TGFR1_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of TGFR1_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
XIAP_MOUSE	Xiap	physical	19531477
OCLN_MOUSE	Ocln	physical	15761153
GDF11_MOUSE	Gdf11	physical	16845371
BLMH_HUMAN	BLMH	physical	26496610
CTNA1_HUMAN	CTNNA1	physical	26496610
GALT1_HUMAN	GALNT1	physical	26496610
PDIA3_HUMAN	PDIA3	physical	26496610
HSP7C_HUMAN	HSPA8	physical	26496610
PLXB1_HUMAN	PLXNB1	physical	26496610
PABP2_HUMAN	PABPN1	physical	26496610
PQBP1_HUMAN	PQBP1	physical	26496610
INT7_HUMAN	INTS7	physical	26496610
ACAD8_HUMAN	ACAD8	physical	26496610
MIER1_HUMAN	MIER1	physical	26496610
S35E1_HUMAN	SLC35E1	physical	26496610
NSE2_HUMAN	NSMCE2	physical	26496610
1C07_HUMAN	HLA-C	physical	26496610

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.