SMAD7_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: SMAD7_MOUSE
• UniProt AC: O35253
• Protein Name: Mothers against decapentaplegic homolog 7
• Gene Name: Smad7
• Organism: Mus musculus (Mouse).
• Sequence Length: 426
• Subcellular Localization: Nucleus . Cytoplasm . Interaction with NEDD4L or RNF111 induces translocation from the nucleus to the cytoplasm (PubMed:15496141). TGF-beta stimulates its translocation from the nucleus to the cytoplasm. PDPK1 inhibits its translocation from the nucl
• Protein Description: Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator..
• Protein Sequence: MFRTKRSALVRRLWRSRAPGGEDEEEGVGGGGGGGELRGEGATDGRAYGAGGGGAGRAGCCLGKAVRGAKGHHHPHPPTSGAGAAGGAEADLKALTHSVLKKLKERQLELLLQAVESRGGTRTACLLLPGRLDCRLGPGAPASAQPAQPPSSYSLPLLLCKVFRWPDLRHSSEVKRLCCCESYGKINPELVCCNPHHLSRLCELESPPPPYSRYPMDFLKPTAGCPDAVPSSAETGGTNYLAPGGLSDSQLLLEPGDRSHWCVVAYWEEKTRVGRLYCVQEPSLDIFYDLPQGNGFCLGQLNSDNKSQLVQKVRSKIGCGIQLTREVDGVWVYNRSSYPIFIKSATLDNPDSRTLLVHKVFPGFSIKAFDYEKAYSLQRPNDHEFMQQPWTGFTVQISFVKGWGQCYTRQFISSCPCWLEVIFNSR

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
64	Acetylation	RAGCCLGKAVRGAKG CCHHHHHHHHHCCCC	30.66	60389
70	Acetylation	GKAVRGAKGHHHPHP HHHHHCCCCCCCCCC	62.69	60391
212	Phosphorylation	ESPPPPYSRYPMDFL CCCCCCCCCCCCCCC	30.98	-
249	Phosphorylation	APGGLSDSQLLLEPG CCCCCCCCCEEECCC	21.25	22817900

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
-	K	Ubiquitination	E3 ubiquitin ligase	Smurf2	A2A5Z6	PMID:22199232

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
249	S	Phosphorylation	Phosphorylation on Ser-249 does not affect its stability, nuclear localization or inhibitory function in TGFB signaling; however it affects its ability to regulate transcription.	11278814

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of SMAD7_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
ACV1B_HUMAN	ACVR1B	physical	9738003
TGFR1_HUMAN	TGFBR1	physical	9738003
YAP1_HUMAN	YAP1	physical	12118366
AXIN1_MOUSE	Axin1	physical	16601693
SMUF1_MOUSE	Smurf1	physical	15173588
SMUF2_MOUSE	Smurf2	physical	15173588
BMR1A_MOUSE	Bmpr1a	physical	20663871
BMPR2_MOUSE	Bmpr2	physical	20663871
ACVR1_MOUSE	Acvr1	physical	20663871
PEX6_MOUSE	Pex6	physical	14651998
4ET_MOUSE	Eif4enif1	physical	14651998
ZMYM2_MOUSE	Zmym2	physical	14651998
PRD16_MOUSE	Prdm16	physical	17467076
CBLB_MOUSE	Cblb	physical	23709694

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Phosphorylation of Smad7 at Ser-249 does not interfere with itsinhibitory role in transforming growth factor-beta-dependent signalingbut affects Smad7-dependent transcriptional activation.";
Pulaski L., Landstrom M., Heldin C.-H., Souchelnytskyi S.;
J. Biol. Chem. 276:14344-14349(2001).
Cited for: PHOSPHORYLATION AT SER-249, MUTAGENESIS OF SER-249, AND SUBCELLULARLOCATION.
PubMed: 11278814