4ET_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: 4ET_MOUSE
• UniProt AC: Q9EST3
• Protein Name: Eukaryotic translation initiation factor 4E transporter
• Gene Name: Eif4enif1
• Organism: Mus musculus (Mouse).
• Sequence Length: 983
• Subcellular Localization: Cytoplasm . Nucleus . Nucleus, PML body . Nucleus speckle . Predominantly cytoplasmic. Shuttles between the nucleus and the cytoplasm in a CRM1-dependent manner. Localization to nuclear foci and speckles requires active transcription.
• Protein Description: Nucleoplasmic shuttling protein, which inhibits translation initiation. Mediates the nuclear import of EIF4E by a piggy-back mechanism..
• Protein Sequence: MEKSVAETENGDAFLELKKLPTSKSPHRYTKEELLDIKERPYSKQRPSCLSEKYDSDGVWDPEKWHASLYPASGRSSPVESLKKESESDRPSLVRRIADPRERVKEDDLDVVLSPQRRSFGGGCHVTAAVSSRRSGSPLEKDSDGLRLLGGRRIGSGRIISARAFEKDHRLSDKDLRDLRDRDRERDYKDKRFRREFGDSKRVFGERRRNDSYTEEEPEWFSAGPTSQSETIELTGFDDKILEEDHKGRKRTRRRTASVKEGIVECNGGVAEEDEVEVILAQEPSADQEVPRDVILPEQSPGEFDFNEFFNLDKVPCLASMIEDVLGEGSVSASRFSRWFSNPSRSGSRSSSLGSTPHEELERLAGLEQAVLSPGQNSGNYFAPIPSEDHAENKVDILEMLQKAKVDLKPLLSSLSANKEKLKESSHSGVVLSVEEVEAGLKGLKVDQQMKNSTPFMAEHLEETLSAASSNRQLKKDGDMTAFNKLVNTMKASGTLPTQPKVSRNVESHLLAPAEIPGQPVSKNILQELLGQPVQRPASSNLLSGLMGSLEATASLLSQRAPSPPMSQVFRTQAASADYLHPRIPSPIGFPSGPQQLLGDPFQGMRKPMSPVSAQMSQLELQQAALEGLALPHDLAVQTAPFYQPGFSKPQVDRTRDGLRNRQQRMSKSPAPMHGGNSSSPAPAASITSMLSPSFTPTSVIRKMYESREKTKEEMAPGMVVPGDGKEDTQKTSEENLLSSNPIPNTDQDSSTTNPKLSTLQRSSCSTPLSQTSRYTKEQDYRPKTAGRKTPTLASPVPGTPFLRPTHQVPLVPHVPIVRPAHQLHPGLVQRLIAQGVHPQHLPSLLQAGVLPPGIDMAPLQGLSGPLLGQPLYPLVSAASHPLLNPRPGTPLHLAVMQQQLQRSVLHPPGSSSQAAAISVQTPQNVPSRSGMPHMHSQLEHRTSQRSSSPVGLAKWFGSDVLQQPLPSMPTKVISVDELEYRQ

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
4	Phosphorylation	----MEKSVAETENG ----CCCCHHCCCCC	17.16	-
23	Phosphorylation	ELKKLPTSKSPHRYT EEECCCCCCCCCCCC	29.24	26824392
25	Phosphorylation	KKLPTSKSPHRYTKE ECCCCCCCCCCCCHH	26.21	25266776
48	Phosphorylation	PYSKQRPSCLSEKYD CCCCCCCCHHCCCCC	29.85	29514104
51	Phosphorylation	KQRPSCLSEKYDSDG CCCCCHHCCCCCCCC	36.05	29514104
68	Phosphorylation	DPEKWHASLYPASGR CHHHHEEEECCCCCC	18.62	27742792
70	Phosphorylation	EKWHASLYPASGRSS HHHEEEECCCCCCCC	8.34	27742792
73	Phosphorylation	HASLYPASGRSSPVE EEEECCCCCCCCCHH	30.18	8345195
76	Phosphorylation	LYPASGRSSPVESLK ECCCCCCCCCHHHHH	42.38	27087446
77	Phosphorylation	YPASGRSSPVESLKK CCCCCCCCCHHHHHH	31.44	25521595
81	Phosphorylation	GRSSPVESLKKESES CCCCCHHHHHHHCCC	45.80	27742792
86	Phosphorylation	VESLKKESESDRPSL HHHHHHHCCCCCHHH	51.81	24719451
88	Phosphorylation	SLKKESESDRPSLVR HHHHHCCCCCHHHHH	48.47	24719451
114	Phosphorylation	DDLDVVLSPQRRSFG CCCCEEECCCCCCCC	13.84	43373417
119	Phosphorylation	VLSPQRRSFGGGCHV EECCCCCCCCCCCEE	30.36	6467063
135	Phosphorylation	AAVSSRRSGSPLEKD EEEECCCCCCCCCCC	42.38	23737553
137	Phosphorylation	VSSRRSGSPLEKDSD EECCCCCCCCCCCCC	27.79	26824392
143	Phosphorylation	GSPLEKDSDGLRLLG CCCCCCCCCCCEECC	44.65	25777480
172	Phosphorylation	FEKDHRLSDKDLRDL HHCCCCCCHHHHHHH	42.44	68996091
212	Phosphorylation	GERRRNDSYTEEEPE CCCCCCCCCCCCCCC	37.19	118599
213	Phosphorylation	ERRRNDSYTEEEPEW CCCCCCCCCCCCCCH	22.49	26745281
214	Phosphorylation	RRRNDSYTEEEPEWF CCCCCCCCCCCCCHH	40.65	10412055
222	Phosphorylation	EEEPEWFSAGPTSQS CCCCCHHHCCCCCCC	33.13	26643407
226	Phosphorylation	EWFSAGPTSQSETIE CHHHCCCCCCCCEEE	38.18	26643407
227	Phosphorylation	WFSAGPTSQSETIEL HHHCCCCCCCCEEEE	34.54	26643407
229	Phosphorylation	SAGPTSQSETIELTG HCCCCCCCCEEEECC	36.15	30635358
231	Phosphorylation	GPTSQSETIELTGFD CCCCCCCEEEECCCC	25.62	26745281
256	Phosphorylation	RKRTRRRTASVKEGI CHHHHHCCHHHHHCE	22.82	158025
258	Phosphorylation	RTRRRTASVKEGIVE HHHHCCHHHHHCEEE	32.86	158045
300	Phosphorylation	DVILPEQSPGEFDFN CCCCCCCCCCCCCHH	32.96	27180971
341	Phosphorylation	SRFSRWFSNPSRSGS HHHHHHHCCCCCCCC	40.65	22942356
344	Phosphorylation	SRWFSNPSRSGSRSS HHHHCCCCCCCCCCC	43.24	28725479
346	Phosphorylation	WFSNPSRSGSRSSSL HHCCCCCCCCCCCCC	45.52	24759943
348	Phosphorylation	SNPSRSGSRSSSLGS CCCCCCCCCCCCCCC	30.18	24759943
350	Phosphorylation	PSRSGSRSSSLGSTP CCCCCCCCCCCCCCC	26.44	25619855
351	Phosphorylation	SRSGSRSSSLGSTPH CCCCCCCCCCCCCCH	28.59	25619855
352	Phosphorylation	RSGSRSSSLGSTPHE CCCCCCCCCCCCCHH	37.69	27742792
355	Phosphorylation	SRSSSLGSTPHEELE CCCCCCCCCCHHHHH	44.02	21082442
356	Phosphorylation	RSSSLGSTPHEELER CCCCCCCCCHHHHHH	27.20	21082442
373	Phosphorylation	GLEQAVLSPGQNSGN CCCHHHCCCCCCCCC	22.01	26239621
378	Phosphorylation	VLSPGQNSGNYFAPI HCCCCCCCCCCCCCC	22.11	26239621
381	Phosphorylation	PGQNSGNYFAPIPSE CCCCCCCCCCCCCCH	12.49	26239621
387	Phosphorylation	NYFAPIPSEDHAENK CCCCCCCCHHHHCCC	57.27	23984901
416	Phosphorylation	KPLLSSLSANKEKLK HHHHHHHHCCHHHHH	31.57	29514104
416	O-linked_Glycosylation	KPLLSSLSANKEKLK HHHHHHHHCCHHHHH	31.57	22517741
454	Phosphorylation	DQQMKNSTPFMAEHL CHHHHCCCHHHHHHH	29.91	25338131
485	Acetylation	GDMTAFNKLVNTMKA CCHHHHHHHHHHHHH	47.56	-
489	Phosphorylation	AFNKLVNTMKASGTL HHHHHHHHHHHCCCC	16.75	23140645
498	Phosphorylation	KASGTLPTQPKVSRN HHCCCCCCCCCCCCC	61.69	23140645
508	Phosphorylation	KVSRNVESHLLAPAE CCCCCCCHHEECCCC	18.36	26239621
522	Phosphorylation	EIPGQPVSKNILQEL CCCCCCCCHHHHHHH	27.03	26239621
563	Phosphorylation	LLSQRAPSPPMSQVF HHHCCCCCCCHHHHH	40.42	26824392
567	Phosphorylation	RAPSPPMSQVFRTQA CCCCCCHHHHHHHHH	29.03	25619855
576	Phosphorylation	VFRTQAASADYLHPR HHHHHHCCCCCCCCC	25.37	28973931
579	Phosphorylation	TQAASADYLHPRIPS HHHCCCCCCCCCCCC	13.46	76465
586	Phosphorylation	YLHPRIPSPIGFPSG CCCCCCCCCCCCCCC	27.12	27087446
592	Phosphorylation	PSPIGFPSGPQQLLG CCCCCCCCCHHHHCC	61.93	21149613
667	Phosphorylation	RNRQQRMSKSPAPMH HHHHHHHHCCCCCCC	31.97	26643407
669	Phosphorylation	RQQRMSKSPAPMHGG HHHHHHCCCCCCCCC	20.98	25335153
678	Phosphorylation	APMHGGNSSSPAPAA CCCCCCCCCCCCCCC	35.15	26643407
679	Phosphorylation	PMHGGNSSSPAPAAS CCCCCCCCCCCCCCH	44.32	26643407
680	Phosphorylation	MHGGNSSSPAPAASI CCCCCCCCCCCCCHH	25.54	9557979
686	Phosphorylation	SSPAPAASITSMLSP CCCCCCCHHHHHHCC	28.62	26643407
688	Phosphorylation	PAPAASITSMLSPSF CCCCCHHHHHHCCCC	12.94	26643407
689	Phosphorylation	APAASITSMLSPSFT CCCCHHHHHHCCCCC	18.96	26643407
692	Phosphorylation	ASITSMLSPSFTPTS CHHHHHHCCCCCCHH	15.17	-
711	Phosphorylation	MYESREKTKEEMAPG HHHHHHHHHHHCCCC	38.65	28059163
751	Phosphorylation	PNTDQDSSTTNPKLS CCCCCCCCCCCCCHH	47.76	-
758	Phosphorylation	STTNPKLSTLQRSSC CCCCCCHHHHHHHCC	32.95	26643407
759	Phosphorylation	TTNPKLSTLQRSSCS CCCCCHHHHHHHCCC	37.03	26643407
763	Phosphorylation	KLSTLQRSSCSTPLS CHHHHHHHCCCCCCH	23.80	23527152
764	Phosphorylation	LSTLQRSSCSTPLSQ HHHHHHHCCCCCCHH	17.80	26643407
766	Phosphorylation	TLQRSSCSTPLSQTS HHHHHCCCCCCHHHC	34.70	23527152
767	Phosphorylation	LQRSSCSTPLSQTSR HHHHCCCCCCHHHCC	32.35	24453211
774	Dimethylation	TPLSQTSRYTKEQDY CCCHHHCCCCCCCCC	46.67	-
790	Phosphorylation	PKTAGRKTPTLASPV CCCCCCCCCCCCCCC	21.83	21082442
792	Phosphorylation	TAGRKTPTLASPVPG CCCCCCCCCCCCCCC	39.94	29895711
795	Phosphorylation	RKTPTLASPVPGTPF CCCCCCCCCCCCCCC	29.55	29895711
890	Phosphorylation	LLNPRPGTPLHLAVM CCCCCCCCHHHHHHH	25.92	30482847
919	Phosphorylation	SSQAAAISVQTPQNV HHHCEEEEEECCCCC	11.93	-
944	Phosphorylation	SQLEHRTSQRSSSPV HHHHHHCCCCCCCCC	24.45	29899451
947	Phosphorylation	EHRTSQRSSSPVGLA HHHCCCCCCCCCHHH	27.77	28833060
948	Phosphorylation	HRTSQRSSSPVGLAK HHCCCCCCCCCHHHH	40.63	28833060
949	Phosphorylation	RTSQRSSSPVGLAKW HCCCCCCCCCHHHHH	25.50	27087446
975	Phosphorylation	SMPTKVISVDELEYR CCCCEEEEHHHCCCC	26.88	101664435

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
114	S	Phosphorylation	Kinase	MAPK1	P63085	GPS

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of 4ET_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of 4ET_MOUSE !!

Protein-Protein Interaction

Oops, there are no PPI records of 4ET_MOUSE !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Large-scale phosphorylation analysis of mouse liver.";
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-77, AND MASSSPECTROMETRY.
PubMed: 17242355

"Phosphoproteomic analysis of the developing mouse brain.";
Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.;
Mol. Cell. Proteomics 3:1093-1101(2004).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-586, AND MASSSPECTROMETRY.
PubMed: 15345747