dbPTM

NCF1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	NCF1_HUMAN
UniProt AC	P14598
Protein Name	Neutrophil cytosol factor 1
Gene Name	NCF1
Organism	Homo sapiens (Human).
Sequence Length	390
Subcellular Localization	Cytoplasm, cytosol . Membrane Peripheral membrane protein Cytoplasmic side .
Protein Description	NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production)..
Protein Sequence	MGDTFIRHIALLGFEKRFVPSQHYVYMFLVKWQDLSEKVVYRRFTEIYEFHKTLKEMFPIEAGAINPENRIIPHLPAPKWFDGQRAAENRQGTLTEYCSTLMSLPTKISRCPHLLDFFKVRPDDLKLPTDNQTKKPETYLMPKDGKSTATDITGPIILQTYRAIANYEKTSGSEMALSTGDVVEVVEKSESGWWFCQMKAKRGWIPASFLEPLDSPDETEDPEPNYAGEPYVAIKAYTAVEGDEVSLLEGEAVEVIHKLLDGWWVIRKDDVTGYFPSMYLQKSGQDVSQAQRQIKRGAPPRRSSIRNAHSIHQRSRKRLSQDAYRRNSVRFLQQRRRQARPGPQSPGSPLEEERQTQRSKPQPAVPPRPSADLILNRCSESTKRKLASAV

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
41	Phosphorylation	DLSEKVVYRRFTEIY HCCHHHHHHHHHHHH	10.08	18083107
45	Phosphorylation	KVVYRRFTEIYEFHK HHHHHHHHHHHHHHH	21.47	28857561
48	Phosphorylation	YRRFTEIYEFHKTLK HHHHHHHHHHHHHHH	13.30	19060867
93	Phosphorylation	AAENRQGTLTEYCST HHHHCCCCHHHHHHH	23.36	25072903
95	Phosphorylation	ENRQGTLTEYCSTLM HHCCCCHHHHHHHHH	25.42	25072903
97	Phosphorylation	RQGTLTEYCSTLMSL CCCCHHHHHHHHHCC	7.31	25072903
100	Phosphorylation	TLTEYCSTLMSLPTK CHHHHHHHHHCCCHH	18.74	25072903
103	Phosphorylation	EYCSTLMSLPTKISR HHHHHHHCCCHHHHC	34.02	25072903
106	Phosphorylation	STLMSLPTKISRCPH HHHHCCCHHHHCCHH	46.43	25072903
133	Phosphorylation	KLPTDNQTKKPETYL CCCCCCCCCCCCEEE	47.49	17217339
138	Phosphorylation	NQTKKPETYLMPKDG CCCCCCCEEECCCCC	30.40	23532336
139	Phosphorylation	QTKKPETYLMPKDGK CCCCCCEEECCCCCC	10.19	-
153	Phosphorylation	KSTATDITGPIILQT CCCCCCCCHHHHHHH	39.45	17217339
173	Phosphorylation	NYEKTSGSEMALSTG CCCCCCCCEEEECCC	24.78	24114839
178	Phosphorylation	SGSEMALSTGDVVEV CCCEEEECCCCEEEE	22.32	24114839
179	Phosphorylation	GSEMALSTGDVVEVV CCEEEECCCCEEEEE	37.95	24114839
208	Phosphorylation	KRGWIPASFLEPLDS CCCCEEHHHCCCCCC	25.63	11535139
226	Phosphorylation	TEDPEPNYAGEPYVA CCCCCCCCCCCCEEE	27.05	23532336
246	Phosphorylation	AVEGDEVSLLEGEAV EECCCEEEEECCCHH	25.78	30108239
279	Phosphorylation	TGYFPSMYLQKSGQD CCCCCHHEEHHCCCC	15.43	17217339
283	Phosphorylation	PSMYLQKSGQDVSQA CHHEEHHCCCCHHHH	28.82	11535139
288	Phosphorylation	QKSGQDVSQAQRQIK HHCCCCHHHHHHHHH	28.25	17217339
303	Phosphorylation	RGAPPRRSSIRNAHS CCCCCCHHHHHCHHH	31.25	12056906
304	Phosphorylation	GAPPRRSSIRNAHSI CCCCCHHHHHCHHHH	24.61	12056906
310	Phosphorylation	SSIRNAHSIHQRSRK HHHHCHHHHHHHHHH	21.25	22460559
315	Phosphorylation	AHSIHQRSRKRLSQD HHHHHHHHHHHHCHH	35.27	12056906
320	Phosphorylation	QRSRKRLSQDAYRRN HHHHHHHCHHHHHHH	30.01	28348404
324	Phosphorylation	KRLSQDAYRRNSVRF HHHCHHHHHHHHHHH	20.56	23532336
328	Phosphorylation	QDAYRRNSVRFLQQR HHHHHHHHHHHHHHH	16.92	12056906
345	Phosphorylation	QARPGPQSPGSPLEE HCCCCCCCCCCCHHH	34.07	20164059
348	Phosphorylation	PGPQSPGSPLEEERQ CCCCCCCCCHHHHHH	29.69	23401153
356	Phosphorylation	PLEEERQTQRSKPQP CHHHHHHHHHCCCCC	31.97	28450419
359	Phosphorylation	EERQTQRSKPQPAVP HHHHHHHCCCCCCCC	38.58	12056906
370	Phosphorylation	PAVPPRPSADLILNR CCCCCCCCHHHHHHC	35.38	12056906
379	Phosphorylation	DLILNRCSESTKRKL HHHHHCCCHHHHHHH	31.20	7657821
381	Phosphorylation	ILNRCSESTKRKLAS HHHCCCHHHHHHHHH	23.96	27251275
382	Phosphorylation	LNRCSESTKRKLASA HHCCCHHHHHHHHHC	30.71	27251275

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
133	T	Phosphorylation	Kinase	IRAK4	Q9NWZ3	PSP
138	T	Phosphorylation	Kinase	IRAK4	Q9NWZ3	PSP
153	T	Phosphorylation	Kinase	IRAK4	Q9NWZ3	PSP
208	S	Phosphorylation	Kinase	CK2_GROUP	-	PhosphoELM
208	S	Phosphorylation	Kinase	CSNK2A1	P68400	GPS
208	S	Phosphorylation	Kinase	CK2-FAMILY	-	GPS
283	S	Phosphorylation	Kinase	CK2-FAMILY	-	GPS
283	S	Phosphorylation	Kinase	CSNK2A1	P68400	GPS
283	S	Phosphorylation	Kinase	CK2_GROUP	-	PhosphoELM
288	S	Phosphorylation	Kinase	IRAK4	Q9NWZ3	PSP
303	S	Phosphorylation	Kinase	PRKCA	P17252	GPS
303	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
303	S	Phosphorylation	Kinase	PRKCZ	Q05513	GPS
303	S	Phosphorylation	Kinase	PRKCD	Q05655	GPS
303	S	Phosphorylation	Kinase	PRKCB	P05771-2	GPS
303	S	Phosphorylation	Kinase	PKA_GROUP	-	PhosphoELM
304	S	Phosphorylation	Kinase	PRKCD	Q05655	GPS
304	S	Phosphorylation	Kinase	PRKCB	P05771-2	GPS
304	S	Phosphorylation	Kinase	PRKCA	P17252	GPS
304	S	Phosphorylation	Kinase	PKB_GROUP	-	PhosphoELM
304	S	Phosphorylation	Kinase	AKT1	P31749	PSP
304	S	Phosphorylation	Kinase	PRKCZ	Q05513	GPS
304	S	Phosphorylation	Kinase	AKT-FAMILY	-	GPS
315	S	Phosphorylation	Kinase	PRKCB	P05771-2	GPS
315	S	Phosphorylation	Kinase	PRKCZ	Q05513	GPS
315	S	Phosphorylation	Kinase	PRKCA	P17252	GPS
315	S	Phosphorylation	Kinase	PRKCD	Q05655	GPS
320	S	Phosphorylation	Kinase	PRKCA	P17252	GPS
320	S	Phosphorylation	Kinase	PRKACA	P17612	GPS
320	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
320	S	Phosphorylation	Kinase	PKA_GROUP	-	PhosphoELM
320	S	Phosphorylation	Kinase	PRKCD	Q05655	GPS
320	S	Phosphorylation	Kinase	PRKCB	P05771-2	GPS
320	S	Phosphorylation	Kinase	IRAK4	Q9NWZ3	PSP
328	S	Phosphorylation	Kinase	AKT-FAMILY	-	GPS
328	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
328	S	Phosphorylation	Kinase	PKA_GROUP	-	PhosphoELM
328	S	Phosphorylation	Kinase	PKB_GROUP	-	PhosphoELM
328	S	Phosphorylation	Kinase	PRKCD	Q05655	GPS
328	S	Phosphorylation	Kinase	AKT1	P31749	PSP
328	S	Phosphorylation	Kinase	PRKCB	P05771-2	GPS
328	S	Phosphorylation	Kinase	PRKACA	P17612	GPS
328	S	Phosphorylation	Kinase	PRKCA	P17252	GPS
345	S	Phosphorylation	Kinase	IRAK4	Q9NWZ3	PSP
345	S	Phosphorylation	Kinase	MAPK1	P28482	GPS
345	S	Phosphorylation	Kinase	MAPK3	P27361	GPS
345	S	Phosphorylation	Kinase	MAPK14	Q16539	GPS
345	S	Phosphorylation	Kinase	ROCK2	O75116	PSP
348	S	Phosphorylation	Kinase	CK2-FAMILY	-	GPS
348	S	Phosphorylation	Kinase	PKA_GROUP	-	PhosphoELM
348	S	Phosphorylation	Kinase	CK2_GROUP	-	PhosphoELM
348	S	Phosphorylation	Kinase	CSNK2A1	P68400	GPS
348	S	Phosphorylation	Kinase	IRAK4	Q9NWZ3	PSP
348	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
348	S	Phosphorylation	Kinase	PKCD	Q05655	PSP
348	S	Phosphorylation	Kinase	PRKCA	P17252	GPS
348	S	Phosphorylation	Kinase	MAPK3	P27361	GPS
348	S	Phosphorylation	Kinase	MAPK14	Q16539	GPS
356	T	Phosphorylation	Kinase	IRAK4	Q9NWZ3	PSP
359	S	Phosphorylation	Kinase	PRKACA	P17612	GPS
359	S	Phosphorylation	Kinase	PRKCB	P05771-2	GPS
359	S	Phosphorylation	Kinase	PKA_GROUP	-	PhosphoELM
359	S	Phosphorylation	Kinase	PRKCD	Q05655	GPS
359	S	Phosphorylation	Kinase	IRAK4	Q9NWZ3	PSP
359	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
359	S	Phosphorylation	Kinase	PKCA	P05696	PSP
359	S	Phosphorylation	Kinase	PRKCA	P17252	GPS
370	S	Phosphorylation	Kinase	PRKACA	P17612	GPS
370	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
370	S	Phosphorylation	Kinase	PRKCB	P05771-2	GPS
370	S	Phosphorylation	Kinase	PKA_GROUP	-	PhosphoELM
370	S	Phosphorylation	Kinase	PRKCA	P17252	GPS
370	S	Phosphorylation	Kinase	IRAK4	Q9NWZ3	PSP
370	S	Phosphorylation	Kinase	PKCA	P05696	PSP
370	S	Phosphorylation	Kinase	PRKCD	Q05655	GPS
379	S	Phosphorylation	Kinase	PRKCA	P17252	GPS
379	S	Phosphorylation	Kinase	PRKCD	Q05655	GPS
379	S	Phosphorylation	Kinase	PRKCB	P05771-2	GPS

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of NCF1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of NCF1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
TRIM9_HUMAN	TRIM9	physical	16189514
TF65_HUMAN	RELA	physical	12618429
NCF4_HUMAN	NCF4	physical	11796733
NCF1_HUMAN	NCF1	physical	7938008
CY24A_HUMAN	CYBA	physical	7938008
NCF2_HUMAN	NCF2	physical	7938008
NCF2_HUMAN	NCF2	physical	10486263
NCF2_HUMAN	NCF2	physical	11733522
KHDR1_HUMAN	KHDRBS1	physical	8766817
NCF4_HUMAN	NCF4	physical	11258927
TRAF4_HUMAN	TRAF4	physical	12023963
KPCB_HUMAN	PRKCB	physical	11120743
CSK2B_HUMAN	CSNK2B	physical	11485312
NCF2_HUMAN	NCF2	physical	12169629
IL4RA_HUMAN	IL4R	physical	10369419
TRAF4_HUMAN	TRAF4	physical	16052631
NCF2_HUMAN	NCF2	physical	16052631
NOXA1_HUMAN	NOXA1	physical	16052631
LYAG_HUMAN	GAA	physical	16052631
SRSF2_HUMAN	SRSF2	physical	16052631
MYPC3_HUMAN	MYBPC3	physical	16052631
GEMI_HUMAN	GMNN	physical	16052631
MGA_HUMAN	MGAM	physical	16052631
GPVI_HUMAN	GP6	physical	20946164
GP1BB_HUMAN	GP1BB	physical	20946164
TGFI1_HUMAN	TGFB1I1	physical	20946164
GNAI1_HUMAN	GNAI1	physical	16782902
NCF2_HUMAN	NCF2	physical	16782902
ACTB_HUMAN	ACTB	physical	16375898
SRC8_HUMAN	CTTN	physical	15618548
ACTA_HUMAN	ACTA2	physical	15618548
MYPC1_HUMAN	MYBPC1	physical	16052631
TAGL_HUMAN	TAGLN	physical	22798525
PA24A_HUMAN	PLA2G4A	physical	18765662
SH3G3_HUMAN	SH3GL3	physical	28514442
NF2L2_HUMAN	NFE2L2	physical	28939422

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
233700	Granulomatous disease, chronic, cytochrome-b-positive 1, autosomal recessive (CGD1)
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
DB00514	Dextromethorphan

Regulatory Network of NCF1_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Phosphorylation analysis of primary human T lymphocytes usingsequential IMAC and titanium oxide enrichment."; Carrascal M., Ovelleiro D., Casas V., Gay M., Abian J.; J. Proteome Res. 7:5167-5176(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-348, AND MASSSPECTROMETRY.	19367720 [Abstract]
"The phosphorylation of the respiratory burst oxidase componentp47phox during neutrophil activation. Phosphorylation of sitesrecognized by protein kinase C and by proline-directed kinases."; el Benna J., Faust L.P., Babior B.M.; J. Biol. Chem. 269:23431-23436(1994). Cited for: PHOSPHORYLATION AT SER-303; SER-304; SER-320; SER-328; SER-345 ANDSER-348.	8089108 [Abstract]