NCF4_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: NCF4_HUMAN
• UniProt AC: Q15080
• Protein Name: Neutrophil cytosol factor 4
• Gene Name: NCF4
• Organism: Homo sapiens (Human).
• Sequence Length: 339
• Subcellular Localization: Cytoplasm, cytosol . Endosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Membrane ; Peripheral membrane protein .
• Protein Description: Component of the NADPH-oxidase, a multicomponent enzyme system responsible for the oxidative burst in which electrons are transported from NADPH to molecular oxygen, generating reactive oxidant intermediates. It may be important for the assembly and/or activation of the NADPH-oxidase complex..
• Protein Sequence: MAVAQQLRAESDFEQLPDDVAISANIADIEEKRGFTSHFVFVIEVKTKGGSKYLIYRRYRQFHALQSKLEERFGPDSKSSALACTLPTLPAKVYVGVKQEIAEMRIPALNAYMKSLLSLPVWVLMDEDVRIFFYQSPYDSEQVPQALRRLRPRTRKVKSVSPQGNSVDRMAAPRAEALFDFTGNSKLELNFKAGDVIFLLSRINKDWLEGTVRGATGIFPLSFVKILKDFPEEDDPTNWLRCYYYEDTISTIKDIAVEEDLSSTPLLKDLLELTRREFQREDIALNYRDAEGDLVRLLSDEDVALMVRQARGLPSQKRLFPWKLHITQKDNYRVYNTMP

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
11	Phosphorylation	AQQLRAESDFEQLPD HHHHHHHCCHHHCCC	46.76	-
23	Phosphorylation	LPDDVAISANIADIE CCCCEEEECCCCCHH	12.91	-
47	Phosphorylation	VFVIEVKTKGGSKYL EEEEEEEECCCCEEE	39.85	-
51	Phosphorylation	EVKTKGGSKYLIYRR EEEECCCCEEEEEEE	27.13	-
53	Phosphorylation	KTKGGSKYLIYRRYR EECCCCEEEEEEEHH	10.46	-
154	Phosphorylation	LRRLRPRTRKVKSVS HHHHCCCCCCCCCCC	37.21	15035643
159	Phosphorylation	PRTRKVKSVSPQGNS CCCCCCCCCCCCCCC	30.71	28450419
161	Phosphorylation	TRKVKSVSPQGNSVD CCCCCCCCCCCCCCH	20.71	23401153
161 (in isoform 3)	Phosphorylation	-	20.71	27251275
166	Phosphorylation	SVSPQGNSVDRMAAP CCCCCCCCCHHCCCC	32.02	28450419
166 (in isoform 3)	Phosphorylation	-	32.02	27251275
201	Phosphorylation	GDVIFLLSRINKDWL CCEEEEEEECCHHHH	33.01	24114839
225 (in isoform 1)	Ubiquitination	-	40.07	21890473
225	Ubiquitination	IFPLSFVKILKDFPE EEEHHHHHHHHCCCC	40.07	21890473
225	Ubiquitination	IFPLSFVKILKDFPE EEEHHHHHHHHCCCC	40.07	21890473
228	Ubiquitination	LSFVKILKDFPEEDD HHHHHHHHCCCCCCC	61.88	-
243	Phosphorylation	PTNWLRCYYYEDTIS CCCCEEEEEECCHHH	11.57	-
245	Phosphorylation	NWLRCYYYEDTISTI CCEEEEEECCHHHHH	4.85	-
264	Phosphorylation	VEEDLSSTPLLKDLL HHCCCCCCHHHHHHH	17.99	24719451
274	Phosphorylation	LKDLLELTRREFQRE HHHHHHHHHHHHHHH	20.50	27542207
275 (in isoform 3)	Phosphorylation	-	49.08	25690035
276 (in isoform 3)	Phosphorylation	-	43.79	25690035
299	Phosphorylation	GDLVRLLSDEDVALM CCEEEECCHHHHHHH	43.47	27251275
315	Phosphorylation	RQARGLPSQKRLFPW HHHCCCCCCCCCCCE	54.07	8280052
317	2-Hydroxyisobutyrylation	ARGLPSQKRLFPWKL HCCCCCCCCCCCEEE	56.24	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
154	T	Phosphorylation	Kinase	PRKCA	P17252	GPS
154	T	Phosphorylation	Kinase	PKCA	P05696	PSP
154	T	Phosphorylation	Kinase	PKC-FAMILY	-	GPS
154	T	Phosphorylation	Kinase	PKC_GROUP	-	PhosphoELM
315	S	Phosphorylation	Kinase	PRKCA	P17252	GPS
315	S	Phosphorylation	Kinase	PKC-FAMILY	-	GPS

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of NCF4_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of NCF4_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
XRCC6_HUMAN	XRCC6	physical	9914162
NCF2_HUMAN	NCF2	physical	9083043
NCF1_HUMAN	NCF1	physical	9083043
NCF2_HUMAN	NCF2	physical	12887891
NCF2_HUMAN	NCF2	physical	12813044

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• 613960: Granulomatous disease, chronic, cytochrome-b-positive 3, autosomal recessive (CGD3)
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• DB00514: Dextromethorphan

Related Literatures of Post-Translational Modification

Phosphorylation

"p40(phox) is phosphorylated on threonine 154 and serine 315 duringactivation of the phagocyte NADPH oxidase. Implication of a proteinkinase c-type kinase in the phosphorylation process.";
Bouin A.-P., Grandvaux N., Vignais P.V., Fuchs A.;
J. Biol. Chem. 273:30097-30103(1998).
Cited for: PHOSPHORYLATION AT THR-154 AND SER-315, AND MUTAGENESIS OF THR-154;THR-211; THR-251; THR-274; SER-315 AND THR-327.
PubMed: 9804763