dbPTM

ACTA_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	ACTA_HUMAN
UniProt AC	P62736
Protein Name	Actin, aortic smooth muscle
Gene Name	ACTA2
Organism	Homo sapiens (Human).
Sequence Length	377
Subcellular Localization	Cytoplasm, cytoskeleton.
Protein Description	Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells..
Protein Sequence	MCEEEDSTALVCDNGSGLCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHSFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDEAGPSIVHRKCF

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MCEEEDSTA ------CCCCCCCCE	10.16	6725286
3	Acetylation	-----MCEEEDSTAL -----CCCCCCCCEE	64.22	22223895
35	Phosphorylation	APRAVFPSIVGRPRH CCCCCCHHHCCCCCC	20.59	30266825
46	Oxidation	RPRHQGVMVGMGQKD CCCCCCEEEECCCCC	2.49	-
49	Oxidation	HQGVMVGMGQKDSYV CCCEEEECCCCCCCC	3.32	-
52	Acetylation	VMVGMGQKDSYVGDE EEEECCCCCCCCCCH	42.80	7213953
52	Methylation	VMVGMGQKDSYVGDE EEEECCCCCCCCCCH	42.80	-
52	Ubiquitination	VMVGMGQKDSYVGDE EEEECCCCCCCCCCH	42.80	21890473
54	Phosphorylation	VGMGQKDSYVGDEAQ EECCCCCCCCCCHHH	28.84	23401153
55	Phosphorylation	GMGQKDSYVGDEAQS ECCCCCCCCCCHHHC	20.70	27273156
62	Phosphorylation	YVGDEAQSKRGILTL CCCCHHHCCCCEEEE	31.22	23401153
63	Sumoylation	VGDEAQSKRGILTLK CCCHHHCCCCEEEEE	42.43	-
63	Acetylation	VGDEAQSKRGILTLK CCCHHHCCCCEEEEE	42.43	7215567
63	Sumoylation	VGDEAQSKRGILTLK CCCHHHCCCCEEEEE	42.43	-
63	Ubiquitination	VGDEAQSKRGILTLK CCCHHHCCCCEEEEE	42.43	21890473
68	Phosphorylation	QSKRGILTLKYPIEH HCCCCEEEEECEECC	21.20	22617229
70	Acetylation	KRGILTLKYPIEHGI CCCEEEEECEECCCC	43.69	25825284
70	Methylation	KRGILTLKYPIEHGI CCCEEEEECEECCCC	43.69	-
70	Ubiquitination	KRGILTLKYPIEHGI CCCEEEEECEECCCC	43.69	21890473
71	Phosphorylation	RGILTLKYPIEHGII CCEEEEECEECCCCC	16.74	18180459
75	Methylation	TLKYPIEHGIITNWD EEECEECCCCCCCHH	33.21	30626964
79	Phosphorylation	PIEHGIITNWDDMEK EECCCCCCCHHHHHH	28.47	36012735
84	Sulfoxidation	IITNWDDMEKIWHHS CCCCHHHHHHHHHHH	5.39	30846556
86	Acetylation	TNWDDMEKIWHHSFY CCHHHHHHHHHHHHH	44.45	23236377
86	Methylation	TNWDDMEKIWHHSFY CCHHHHHHHHHHHHH	44.45	-
91	Phosphorylation	MEKIWHHSFYNELRV HHHHHHHHHHHCCCC	19.28	26657352
93	Phosphorylation	KIWHHSFYNELRVAP HHHHHHHHHCCCCCC	15.67	27259358
97	Methylation	HSFYNELRVAPEEHP HHHHHCCCCCCCCCC	19.17	-
105	Phosphorylation	VAPEEHPTLLTEAPL CCCCCCCCCCCCCCC	35.99	26437602
108	Phosphorylation	EEHPTLLTEAPLNPK CCCCCCCCCCCCCCC	32.96	21406692
115	Acetylation	TEAPLNPKANREKMT CCCCCCCCCCHHHHH	58.16	30585401
115	Ubiquitination	TEAPLNPKANREKMT CCCCCCCCCCHHHHH	58.16	21890473
122	Phosphorylation	KANREKMTQIMFETF CCCHHHHHHHHHHHC	26.47	-
128	Phosphorylation	MTQIMFETFNVPAMY HHHHHHHHCCHHHHH	14.83	-
145	Phosphorylation	IQAVLSLYASGRTTG HHHHHHHHHCCCCEE	8.71	30803931
150	Phosphorylation	SLYASGRTTGIVLDS HHHHCCCCEEEEEEC	32.66	26657352
151	Phosphorylation	LYASGRTTGIVLDSG HHHCCCCEEEEEECC	24.45	26437602
157	Phosphorylation	TTGIVLDSGDGVTHN CEEEEEECCCCCCCC	34.32	10632791
162	Phosphorylation	LDSGDGVTHNVPIYE EECCCCCCCCCCEEC	17.17	26437602
168	Phosphorylation	VTHNVPIYEGYALPH CCCCCCEECCCCHHH	9.38	7561247
171	Phosphorylation	NVPIYEGYALPHAIM CCCEECCCCHHHHHH	7.94	7330091
178	Sulfoxidation	YALPHAIMRLDLAGR CCHHHHHHHHHCCCC	3.45	30846556
188	Phosphorylation	DLAGRDLTDYLMKIL HCCCCCHHHHHHHHH	27.21	28152594
190	Phosphorylation	AGRDLTDYLMKILTE CCCCHHHHHHHHHHH	11.94	26846344
192	Sulfoxidation	RDLTDYLMKILTERG CCHHHHHHHHHHHCC	1.75	31801345
193	Acetylation	DLTDYLMKILTERGY CHHHHHHHHHHHCCC	31.78	22646677
193	Methylation	DLTDYLMKILTERGY CHHHHHHHHHHHCCC	31.78	-
193	Ubiquitination	DLTDYLMKILTERGY CHHHHHHHHHHHCCC	31.78	21890473
196	Phosphorylation	DYLMKILTERGYSFV HHHHHHHHHCCCCCE	27.40	46158449
198	Methylation	LMKILTERGYSFVTT HHHHHHHCCCCCEEH	44.08	-
200	Phosphorylation	KILTERGYSFVTTAE HHHHHCCCCCEEHHC	12.73	54893
201	Phosphorylation	ILTERGYSFVTTAER HHHHCCCCCEEHHCH	18.96	36642193
204	Phosphorylation	ERGYSFVTTAEREIV HCCCCCEEHHCHHHH	20.48	26657352
205	Phosphorylation	RGYSFVTTAEREIVR CCCCCEEHHCHHHHH	22.63	28857561
215	Acetylation	REIVRDIKEKLCYVA HHHHHHHHHHHCEEE	53.75	11921171
215	Ubiquitination	REIVRDIKEKLCYVA HHHHHHHHHHHCEEE	53.75	21890473
217	Acetylation	IVRDIKEKLCYVALD HHHHHHHHHCEEEEE	38.56	7910615
217	Methylation	IVRDIKEKLCYVALD HHHHHHHHHCEEEEE	38.56	-
219	S-nitrosocysteine	RDIKEKLCYVALDFE HHHHHHHCEEEEECC	3.68	-
219	Glutathionylation	RDIKEKLCYVALDFE HHHHHHHCEEEEECC	3.68	22555962
219	S-nitrosylation	RDIKEKLCYVALDFE HHHHHHHCEEEEECC	3.68	21278135
220	Phosphorylation	DIKEKLCYVALDFEN HHHHHHCEEEEECCH	10.13	29396449
231	Phosphorylation	DFENEMATAASSSSL ECCHHHHHHCCCCCC	22.80	26657352
234	Phosphorylation	NEMATAASSSSLEKS HHHHHHCCCCCCCHH	28.43	26657352
235	Phosphorylation	EMATAASSSSLEKSY HHHHHCCCCCCCHHE	21.15	35096073
236	Phosphorylation	MATAASSSSLEKSYE HHHHCCCCCCCHHEE	36.13	26657352
237	Phosphorylation	ATAASSSSLEKSYEL HHHCCCCCCCHHEEC	42.36	29396449
240	Ubiquitination	ASSSSLEKSYELPDG CCCCCCCHHEECCCC	64.73	-
241	Phosphorylation	SSSSLEKSYELPDGQ CCCCCCHHEECCCCC	17.92	29255136
242	Phosphorylation	SSSLEKSYELPDGQV CCCCCHHEECCCCCE	32.33	29255136
251	Phosphorylation	LPDGQVITIGNERFR CCCCCEEEECCCEEC	24.79	26846344
259	S-nitrosocysteine	IGNERFRCPETLFQP ECCCEECCCCHHCCC	3.08	-
259	S-nitrosylation	IGNERFRCPETLFQP ECCCEECCCCHHCCC	3.08	21278135
262	Phosphorylation	ERFRCPETLFQPSFI CEECCCCHHCCCCCC	20.29	-
267	Phosphorylation	PETLFQPSFIGMESA CCHHCCCCCCCHHCC	20.79	46158533
273	Phosphorylation	PSFIGMESAGIHETT CCCCCHHCCCCCCCC	23.93	22210691
280	Phosphorylation	SAGIHETTYNSIMKC CCCCCCCCCHHHHCC	20.09	-
281	Phosphorylation	AGIHETTYNSIMKCD CCCCCCCCHHHHCCC	17.84	3828983
283	Phosphorylation	IHETTYNSIMKCDID CCCCCCHHHHCCCCE	17.41	-
287	S-nitrosocysteine	TYNSIMKCDIDIRKD CCHHHHCCCCEECHH	2.83	-
287	S-nitrosylation	TYNSIMKCDIDIRKD CCHHHHCCCCEECHH	2.83	21278135
293	Malonylation	KCDIDIRKDLYANNV CCCCEECHHHHHCCC	53.04	26320211
293	Ubiquitination	KCDIDIRKDLYANNV CCCCEECHHHHHCCC	53.04	-
296	Nitrated tyrosine	IDIRKDLYANNVLSG CEECHHHHHCCCCCC	19.88	-
296	Phosphorylation	IDIRKDLYANNVLSG CEECHHHHHCCCCCC	19.88	20563989
302	Phosphorylation	LYANNVLSGGTTMYP HHHCCCCCCCCCCCC	30.78	21406692
305	Phosphorylation	NNVLSGGTTMYPGIA CCCCCCCCCCCCCHH	16.35	21406692
306	Phosphorylation	NVLSGGTTMYPGIAD CCCCCCCCCCCCHHH	20.27	21406692
307	Sulfoxidation	VLSGGTTMYPGIADR CCCCCCCCCCCHHHH	3.69	30846556
308	Phosphorylation	LSGGTTMYPGIADRM CCCCCCCCCCHHHHH	9.01	20563997
317	Sumoylation	GIADRMQKEITALAP CHHHHHHHHHHHHCC	41.56	-
317	Acetylation	GIADRMQKEITALAP CHHHHHHHHHHHHCC	41.56	7221445
317	Sumoylation	GIADRMQKEITALAP CHHHHHHHHHHHHCC	41.56	-
317	Ubiquitination	GIADRMQKEITALAP CHHHHHHHHHHHHCC	41.56	21890473
320	Phosphorylation	DRMQKEITALAPSTM HHHHHHHHHHCCCCE	19.40	30266825
325	Phosphorylation	EITALAPSTMKIKII HHHHHCCCCEEEEEE	35.21	23401153
326	Phosphorylation	ITALAPSTMKIKIIA HHHHCCCCEEEEEEC	22.79	46158473
328	Sumoylation	ALAPSTMKIKIIAPP HHCCCCEEEEEECCC	40.72	-
328	Acetylation	ALAPSTMKIKIIAPP HHCCCCEEEEEECCC	40.72	7215575
328	Methylation	ALAPSTMKIKIIAPP HHCCCCEEEEEECCC	40.72	-
328	Sumoylation	ALAPSTMKIKIIAPP HHCCCCEEEEEECCC	40.72	-
328	Ubiquitination	ALAPSTMKIKIIAPP HHCCCCEEEEEECCC	40.72	21890473
330	Sumoylation	APSTMKIKIIAPPER CCCCEEEEEECCCCC	23.65	-
330	Acetylation	APSTMKIKIIAPPER CCCCEEEEEECCCCC	23.65	7692061
330	Sumoylation	APSTMKIKIIAPPER CCCCEEEEEECCCCC	23.65	-
330	Ubiquitination	APSTMKIKIIAPPER CCCCEEEEEECCCCC	23.65	21890473
338	Sumoylation	IIAPPERKYSVWIGG EECCCCCCEEEEECH	39.97	-
350	Phosphorylation	IGGSILASLSTFQQM ECHHHHHHHHHHHHH	21.29	-
364	Phosphorylation	MWISKQEYDEAGPSI HHHCHHHHCCCCCCC	19.31	20068231
370	O-linked_Glycosylation	EYDEAGPSIVHRKCF HHCCCCCCCCCCCCC	36.00	29351928
370	Phosphorylation	EYDEAGPSIVHRKCF HHCCCCCCCCCCCCC	36.00	26657352
375	Ubiquitination	GPSIVHRKCF----- CCCCCCCCCC-----	25.72	-
376	S-nitrosocysteine	PSIVHRKCF------ CCCCCCCCC------	5.02	-
376	S-nitrosylation	PSIVHRKCF------ CCCCCCCCC------	5.02	21278135

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of ACTA_HUMAN !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Reference
46	M	Oxidation	-
Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization.
49	M	Oxidation	-
Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization.
75	H	Methylation	30626964
Methylated at His-75 by SETD3.
86	K	Methylation	-
Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of ACTA_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
TCPD_HUMAN	CCT4	physical	11580270
PCY1B_HUMAN	PCYT1B	physical	11580270
TCPE_HUMAN	CCT5	physical	11580270
MYH9_HUMAN	MYH9	physical	22939629
MYH11_HUMAN	MYH11	physical	22939629
RL10A_HUMAN	RPL10A	physical	22939629
RL18_HUMAN	RPL18	physical	22939629
RS23_HUMAN	RPS23	physical	22939629
RL14_HUMAN	RPL14	physical	22939629
RL7A_HUMAN	RPL7A	physical	22939629
RS15A_HUMAN	RPS15A	physical	22939629
RL17_HUMAN	RPL17	physical	22939629
RS16_HUMAN	RPS16	physical	22939629
RS26_HUMAN	RPS26	physical	22939629
RS4X_HUMAN	RPS4X	physical	22939629
MYH10_HUMAN	MYH10	physical	22939629
ACTC_HUMAN	ACTC1	physical	22939629
MYO1C_HUMAN	MYO1C	physical	22939629
TPM1_HUMAN	TPM1	physical	22939629
RM47_HUMAN	MRPL47	physical	22939629
SYAC_HUMAN	AARS	physical	22939629
FSCN1_HUMAN	FSCN1	physical	22939629
UBAP1_HUMAN	UBAP1	physical	22939629
ZYX_HUMAN	ZYX	physical	22939629
RT02_HUMAN	MRPS2	physical	22939629
RT07_HUMAN	MRPS7	physical	22939629
GSTP1_HUMAN	GSTP1	physical	22939629
SPTN1_HUMAN	SPTAN1	physical	22939629
CNN2_HUMAN	CNN2	physical	22939629
LOX12_HUMAN	ALOX12	physical	17379189

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
	Note=ACTA2 mutations predispose patients to a variety of diffuse and diverse vascular diseases, premature onset coronary artery disease (CAD), premature ischemic strokes and Moyamoya disease.
611788
614042	Moyamoya disease 5 (MYMY5)
613834	Multisystemic smooth muscle dysfunction syndrome (MSMDYS)
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of ACTA_HUMAN

Related Literatures of Post-Translational Modification

Nitration
Reference	PubMed
"The human pituitary nitroproteome: detection of nitrotyrosyl-proteinswith two-dimensional Western blotting, and amino acid sequencedetermination with mass spectrometry."; Zhan X., Desiderio D.M.; Biochem. Biophys. Res. Commun. 325:1180-1186(2004). Cited for: NITRATION [LARGE SCALE ANALYSIS] AT TYR-296, AND MASS SPECTROMETRY.	15555551 [Abstract]
Phosphorylation
Reference	PubMed
"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach."; Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.; Anal. Chem. 81:4493-4501(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-325, AND MASSSPECTROMETRY.	19413330 [Abstract]

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