dbPTM

JAK1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	JAK1_HUMAN
UniProt AC	P23458
Protein Name	Tyrosine-protein kinase JAK1
Gene Name	JAK1
Organism	Homo sapiens (Human).
Sequence Length	1154
Subcellular Localization	Endomembrane system Peripheral membrane protein. Wholly intracellular, possibly membrane associated.
Protein Description	Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. [PubMed: 7615558 Kinase partner for the interleukin (IL)-2 receptor]
Protein Sequence	MQYLNIKEDCNAMAFCAKMRSSKKTEVNLEAPEPGVEVIFYLSDREPLRLGSGEYTAEELCIRAAQACRISPLCHNLFALYDENTKLWYAPNRTITVDDKMSLRLHYRMRFYFTNWHGTNDNEQSVWRHSPKKQKNGYEKKKIPDATPLLDASSLEYLFAQGQYDLVKCLAPIRDPKTEQDGHDIENECLGMAVLAISHYAMMKKMQLPELPKDISYKRYIPETLNKSIRQRNLLTRMRINNVFKDFLKEFNNKTICDSSVSTHDLKVKYLATLETLTKHYGAEIFETSMLLISSENEMNWFHSNDGGNVLYYEVMVTGNLGIQWRHKPNVVSVEKEKNKLKRKKLENKHKKDEEKNKIREEWNNFSYFPEITHIVIKESVVSINKQDNKKMELKLSSHEEALSFVSLVDGYFRLTADAHHYLCTDVAPPLIVHNIQNGCHGPICTEYAINKLRQEGSEEGMYVLRWSCTDFDNILMTVTCFEKSEQVQGAQKQFKNFQIEVQKGRYSLHGSDRSFPSLGDLMSHLKKQILRTDNISFMLKRCCQPKPREISNLLVATKKAQEWQPVYPMSQLSFDRILKKDLVQGEHLGRGTRTHIYSGTLMDYKDDEGTSEEKKIKVILKVLDPSHRDISLAFFEAASMMRQVSHKHIVYLYGVCVRDVENIMVEEFVEGGPLDLFMHRKSDVLTTPWKFKVAKQLASALSYLEDKDLVHGNVCTKNLLLAREGIDSECGPFIKLSDPGIPITVLSRQECIERIPWIAPECVEDSKNLSVAADKWSFGTTLWEICYNGEIPLKDKTLIEKERFYESRCRPVTPSCKELADLMTRCMNYDPNQRPFFRAIMRDINKLEEQNPDIVSEKKPATEVDPTHFEKRFLKRIRDLGEGHFGKVELCRYDPEGDNTGEQVAVKSLKPESGGNHIADLKKEIEILRNLYHENIVKYKGICTEDGGNGIKLIMEFLPSGSLKEYLPKNKNKINLKQQLKYAVQICKGMDYLGSRQYVHRDLAARNVLVESEHQVKIGDFGLTKAIETDKEYYTVKDDRDSPVFWYAPECLMQSKFYIASDVWSFGVTLHELLTYCDSDSSPMALFLKMIGPTHGQMTVTRLVNTLKEGKRLPCPPNCPDEVYQLMRKCWEFQPSNRTSFQNLIEGFEALLK

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MQYLNIKE -------CCCCCHHH	28.37	19369195
3	Phosphorylation	-----MQYLNIKEDC -----CCCCCHHHHH	17.36	19369195
86	Ubiquitination	ALYDENTKLWYAPNR HEECCCCCEEECCCC	48.91	-
100	Ubiquitination	RTITVDDKMSLRLHY CEEEECCCCHHEEEE	26.40	-
112	Phosphorylation	LHYRMRFYFTNWHGT EEEEEEEEEECCCCC	10.10	20068231
114	Phosphorylation	YRMRFYFTNWHGTND EEEEEEEECCCCCCC	26.48	20068231
119	Phosphorylation	YFTNWHGTNDNEQSV EEECCCCCCCCCCCC	26.89	20068231
125	Phosphorylation	GTNDNEQSVWRHSPK CCCCCCCCCCCCCCC	19.91	20068231
141	Ubiquitination	QKNGYEKKKIPDATP CCCCCCCCCCCCCCC	43.95	-
142	Ubiquitination	KNGYEKKKIPDATPL CCCCCCCCCCCCCCC	71.22	-
208	Phosphorylation	AMMKKMQLPELPKDI HHHHHCCCCCCCCCC	2.96	15659558
213	Ubiquitination	MQLPELPKDISYKRY CCCCCCCCCCCHHHC	79.08	21890473
216	Phosphorylation	PELPKDISYKRYIPE CCCCCCCCHHHCCCH	34.43	-
217	Phosphorylation	ELPKDISYKRYIPET CCCCCCCHHHCCCHH	10.38	-
220	Phosphorylation	KDISYKRYIPETLNK CCCCHHHCCCHHHCH	19.37	15659558
224	Phosphorylation	YKRYIPETLNKSIRQ HHHCCCHHHCHHHHH	30.21	19369195
227	Ubiquitination	YIPETLNKSIRQRNL CCCHHHCHHHHHCCH	50.82	21890473
228	Phosphorylation	IPETLNKSIRQRNLL CCHHHCHHHHHCCHH	23.17	21722762
236	Phosphorylation	IRQRNLLTRMRINNV HHHCCHHHHHHHHHH	26.11	19369195
245	Ubiquitination	MRINNVFKDFLKEFN HHHHHHHHHHHHHHC	43.20	21906983
249	Ubiquitination	NVFKDFLKEFNNKTI HHHHHHHHHHCCCCC	61.32	21890473
254	Ubiquitination	FLKEFNNKTICDSSV HHHHHCCCCCCCCCC	40.13	-
259	Phosphorylation	NNKTICDSSVSTHDL CCCCCCCCCCCHHHC	28.38	28857561
260	Phosphorylation	NKTICDSSVSTHDLK CCCCCCCCCCHHHCH	13.56	28857561
267	Acetylation	SVSTHDLKVKYLATL CCCHHHCHHEEHHHH	42.62	27452117
267	Ubiquitination	SVSTHDLKVKYLATL CCCHHHCHHEEHHHH	42.62	-
269	Acetylation	STHDLKVKYLATLET CHHHCHHEEHHHHHH	32.72	19829835
269	Ubiquitination	STHDLKVKYLATLET CHHHCHHEEHHHHHH	32.72	21890473
270	Phosphorylation	THDLKVKYLATLETL HHHCHHEEHHHHHHH	12.45	20068231
273	Phosphorylation	LKVKYLATLETLTKH CHHEEHHHHHHHHHH	23.64	20068231
278	Phosphorylation	LATLETLTKHYGAEI HHHHHHHHHHHCCCH	24.16	20068231
333	Phosphorylation	RHKPNVVSVEKEKNK ECCCCEEEHHHHHHH	21.92	21722762
373	Phosphorylation	FSYFPEITHIVIKES CCCCCCCEEEEEEEC	12.14	26074081
380	Phosphorylation	THIVIKESVVSINKQ EEEEEEECEEEEECC	23.87	26074081
383	Phosphorylation	VIKESVVSINKQDNK EEEECEEEEECCCCC	20.49	26074081
386	Ubiquitination	ESVVSINKQDNKKME ECEEEEECCCCCCEE	58.31	-
397	Phosphorylation	KKMELKLSSHEEALS CCEEEEECCHHHHHH	28.23	26074081
398	Phosphorylation	KMELKLSSHEEALSF CEEEEECCHHHHHHH	45.41	26074081
412	Phosphorylation	FVSLVDGYFRLTADA HHHHHCCEEEECCCC	4.89	-
463	Phosphorylation	EGSEEGMYVLRWSCT CCCCCCEEEEEEEEC	13.74	27642862
468	Phosphorylation	GMYVLRWSCTDFDNI CEEEEEEEECCHHCE	10.74	22468782
470	Phosphorylation	YVLRWSCTDFDNILM EEEEEEECCHHCEEE	33.90	22468782
493	Ubiquitination	EQVQGAQKQFKNFQI HHHCHHHHHHHCEEE	58.11	-
496	Ubiquitination	QGAQKQFKNFQIEVQ CHHHHHHHCEEEEEE	55.12	-
504	Ubiquitination	NFQIEVQKGRYSLHG CEEEEEECCCEECCC	51.00	-
507	Phosphorylation	IEVQKGRYSLHGSDR EEEECCCEECCCCCC	25.01	21214269
508	Phosphorylation	EVQKGRYSLHGSDRS EEECCCEECCCCCCC	16.72	24719451
512	Phosphorylation	GRYSLHGSDRSFPSL CCEECCCCCCCCCCH	21.07	23612710
515	Phosphorylation	SLHGSDRSFPSLGDL ECCCCCCCCCCHHHH	46.64	-
518	Phosphorylation	GSDRSFPSLGDLMSH CCCCCCCCHHHHHHH	42.87	-
524	Phosphorylation	PSLGDLMSHLKKQIL CCHHHHHHHHHHHHH	32.72	24719451
537	Phosphorylation	ILRTDNISFMLKRCC HHHCCCHHHHHHHHC	15.74	28857561
541	Ubiquitination	DNISFMLKRCCQPKP CCHHHHHHHHCCCCC	31.44	-
559	Ubiquitination	SNLLVATKKAQEWQP HHHHHHCCCCCCCCC	36.05	-
560	Ubiquitination	NLLVATKKAQEWQPV HHHHHCCCCCCCCCC	51.45	-
568	Phosphorylation	AQEWQPVYPMSQLSF CCCCCCCCCHHHCCH	10.41	25147952
571	Phosphorylation	WQPVYPMSQLSFDRI CCCCCCHHHCCHHHH	24.38	27251275
574	Phosphorylation	VYPMSQLSFDRILKK CCCHHHCCHHHHHHH	19.91	22817900
581	Ubiquitination	SFDRILKKDLVQGEH CHHHHHHHCCCCCCC	53.50	-
598	Phosphorylation	RGTRTHIYSGTLMDY CCCEEEEEECEEECE	8.08	27642862
605	Phosphorylation	YSGTLMDYKDDEGTS EECEEECEECCCCCC	11.44	-
606	Ubiquitination	SGTLMDYKDDEGTSE ECEEECEECCCCCCH	55.21	-
687	Phosphorylation	HRKSDVLTTPWKFKV ECCCCCCCCCCHHHH	30.57	18491316
688	Phosphorylation	RKSDVLTTPWKFKVA CCCCCCCCCCHHHHH	24.00	18491316
691	Ubiquitination	DVLTTPWKFKVAKQL CCCCCCCHHHHHHHH	36.19	-
696	Ubiquitination	PWKFKVAKQLASALS CCHHHHHHHHHHHHH	49.16	-
708	Ubiquitination	ALSYLEDKDLVHGNV HHHHHHCCCCCCCCC	43.52	-
718	Ubiquitination	VHGNVCTKNLLLARE CCCCCCCHHHHHHHC	40.23	-
736	Ubiquitination	SECGPFIKLSDPGIP CCCCCCEECCCCCCC	42.17	-
738	Phosphorylation	CGPFIKLSDPGIPIT CCCCEECCCCCCCEE	36.44	-
768	Ubiquitination	PECVEDSKNLSVAAD HHHHCCCCCCCEEEC	74.38	-
798	Phosphorylation	EIPLKDKTLIEKERF CCCCCCCCHHCHHHH	43.00	28060719
802	Ubiquitination	KDKTLIEKERFYESR CCCCHHCHHHHHHHC	47.39	-
825	Phosphorylation	KELADLMTRCMNYDP HHHHHHHHHHHCCCC	28.19	30631047
847	Ubiquitination	AIMRDINKLEEQNPD HHHHHHHHHHHHCCC	58.57	-
857	Phosphorylation	EQNPDIVSEKKPATE HHCCCCCCCCCCCCC	43.99	25159151
859	Ubiquitination	NPDIVSEKKPATEVD CCCCCCCCCCCCCCC	57.46	-
860	Ubiquitination	PDIVSEKKPATEVDP CCCCCCCCCCCCCCC	35.51	-
872	Ubiquitination	VDPTHFEKRFLKRIR CCCCHHHHHHHHHHH	47.72	-
888	Ubiquitination	LGEGHFGKVELCRYD HCCCCCCEEEEEEEC	31.43	-
908	Ubiquitination	TGEQVAVKSLKPESG CCCEEEEEECCCCCC	40.09	-
909	Phosphorylation	GEQVAVKSLKPESGG CCEEEEEECCCCCCC	34.72	24719451
911	Ubiquitination	QVAVKSLKPESGGNH EEEEEECCCCCCCCC	54.55	-
923	Ubiquitination	GNHIADLKKEIEILR CCCHHHHHHHHHHHH	49.24	-
924	Ubiquitination	NHIADLKKEIEILRN CCHHHHHHHHHHHHH	71.82	-
939	Ubiquitination	LYHENIVKYKGICTE HHCCCCCEEEEEEEC	37.13	-
941	Ubiquitination	HENIVKYKGICTEDG CCCCCEEEEEEECCC	35.76	-
961	Phosphorylation	LIMEFLPSGSLKEYL EEEECCCCCCHHHHC	43.06	24850871
963	Phosphorylation	MEFLPSGSLKEYLPK EECCCCCCHHHHCCC	40.37	24850871
967	Phosphorylation	PSGSLKEYLPKNKNK CCCCHHHHCCCCCCC	27.07	-
978	Ubiquitination	NKNKINLKQQLKYAV CCCCCCHHHHHHHHH	31.35	-
993	Phosphorylation	QICKGMDYLGSRQYV HHHCCCCHHCCCHHH	12.22	20071362
1018	Ubiquitination	VESEHQVKIGDFGLT EEECCEEEECCCCCC	34.11	-
1022	Phosphorylation	HQVKIGDFGLTKAIE CEEEECCCCCCEEEE	7.92	15659558
1023	Phosphorylation	QVKIGDFGLTKAIET EEEECCCCCCEEEEC	36.86	15659558
1025	Phosphorylation	KIGDFGLTKAIETDK EECCCCCCEEEECCC	20.86	24702127
1026	Ubiquitination	IGDFGLTKAIETDKE ECCCCCCEEEECCCC	53.03	-
1030	Phosphorylation	GLTKAIETDKEYYTV CCCEEEECCCCCEEE	46.38	21945579
1032	Ubiquitination	TKAIETDKEYYTVKD CEEEECCCCCEEECC	55.85	-
1034	Dephosphorylation	AIETDKEYYTVKDDR EEECCCCCEEECCCC	15.35	11201744
1034	Phosphorylation	AIETDKEYYTVKDDR EEECCCCCEEECCCC	15.35	21945579
1035	Dephosphorylation	IETDKEYYTVKDDRD EECCCCCEEECCCCC	13.22	11201744
1035	Phosphorylation	IETDKEYYTVKDDRD EECCCCCEEECCCCC	13.22	21945579
1036	Phosphorylation	ETDKEYYTVKDDRDS ECCCCCEEECCCCCC	21.85	21945579
1038	Ubiquitination	DKEYYTVKDDRDSPV CCCCEEECCCCCCCE	46.22	-
1043	Phosphorylation	TVKDDRDSPVFWYAP EECCCCCCCEEEECC	24.69	-
1076	Phosphorylation	VTLHELLTYCDSDSS CCHHHHHHHCCCCCC	34.43	22210691
1077	Phosphorylation	TLHELLTYCDSDSSP CHHHHHHHCCCCCCC	8.33	22210691
1080	Phosphorylation	ELLTYCDSDSSPMAL HHHHHCCCCCCCHHH	35.51	22210691
1082	Phosphorylation	LTYCDSDSSPMALFL HHHCCCCCCCHHHHH	40.05	22210691
1083	Phosphorylation	TYCDSDSSPMALFLK HHCCCCCCCHHHHHH	24.56	22210691
1095	Phosphorylation	FLKMIGPTHGQMTVT HHHHHCCCCCCHHHH	33.16	29759185
1100	Phosphorylation	GPTHGQMTVTRLVNT CCCCCCHHHHHHHHH	15.73	22210691
1102	Phosphorylation	THGQMTVTRLVNTLK CCCCHHHHHHHHHHH	14.77	22210691
1107	Phosphorylation	TVTRLVNTLKEGKRL HHHHHHHHHHCCCCC	31.56	18491316
1109	Acetylation	TRLVNTLKEGKRLPC HHHHHHHHCCCCCCC	63.48	7695133
1109	Ubiquitination	TRLVNTLKEGKRLPC HHHHHHHHCCCCCCC	63.48	-
1112	Acetylation	VNTLKEGKRLPCPPN HHHHHCCCCCCCCCC	52.43	7695145
1125	Phosphorylation	PNCPDEVYQLMRKCW CCCCHHHHHHHHHHH	8.22	-
1130	Ubiquitination	EVYQLMRKCWEFQPS HHHHHHHHHHHCCCC	29.72	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
515	S	Phosphorylation	Kinase	PRKAA1	Q13131	GPS
518	S	Phosphorylation	Kinase	PRKAA1	Q13131	GPS
1022	Y	Phosphorylation	Kinase	JAK3	P52333	PhosphoELM
1023	Y	Phosphorylation	Kinase	JAK3	P52333	PhosphoELM
1034	Y	Phosphorylation	Kinase	JAK3	P52333	PSP
1035	Y	Phosphorylation	Kinase	JAK3	P52333	PSP
-	K	Ubiquitination	E3 ubiquitin ligase	RNF125	Q96EQ8	PMID:22199232

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of JAK1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of JAK1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
STAT3_HUMAN	STAT3	physical	11751884
ABL2_HUMAN	ABL2	physical	9774693
TEC_HUMAN	TEC	physical	9178903
BTK_HUMAN	BTK	physical	9178903
STAT3_HUMAN	STAT3	physical	11722592
IRS1_HUMAN	IRS1	physical	9492017
IGF1R_HUMAN	IGF1R	physical	9492017
IL2RB_HUMAN	IL2RB	physical	7973659
IL6RB_HUMAN	IL6ST	physical	11468294
STAM2_HUMAN	STAM2	physical	10993906
PTN11_HUMAN	PTPN11	physical	8995399
STA5B_HUMAN	STAT5B	physical	9047382
STA5A_HUMAN	STAT5A	physical	9047382
IL2RB_HUMAN	IL2RB	physical	7973658
IL21R_HUMAN	IL21R	physical	11016959
GRB2_HUMAN	GRB2	physical	11527382
IL9R_HUMAN	IL9R	physical	8756628
TSHR_HUMAN	TSHR	physical	10809230
RAF1_HUMAN	RAF1	physical	9446616
H31T_HUMAN	HIST3H3	physical	21151131
INAR2_HUMAN	IFNAR2	physical	16710296
SOCS1_HUMAN	SOCS1	physical	10707961
SOCS3_HUMAN	SOCS3	physical	10707961
SOCS3_HUMAN	SOCS3	physical	10373548
STAT6_HUMAN	STAT6	physical	9651359
JAK1_HUMAN	JAK1	physical	8041779
STAT1_HUMAN	STAT1	physical	15284024
STAT3_HUMAN	STAT3	physical	15284024
PLCG1_HUMAN	PLCG1	physical	14978237
PA24A_HUMAN	PLA2G4A	physical	8612580
MDM2_HUMAN	MDM2	physical	24413661
DESP_HUMAN	DSP	physical	25852190
FILA2_HUMAN	FLG2	physical	25852190
RN125_HUMAN	RNF125	physical	26027934

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
D09959	Ruxolitinib (USAN/INN)
D09960	Ruxolitinib phosphate (JAN/USAN); Jakafi (TN)
D10315	Momelotinib (USAN/INN)
D10358	Momelotinib dihydrochloride (USAN)
DrugBank
DB08877	Ruxolitinib
DB08895	Tofacitinib

Regulatory Network of JAK1_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Large-scale proteomics analysis of the human kinome."; Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G.,Mann M., Daub H.; Mol. Cell. Proteomics 8:1751-1764(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-3; THR-224; SER-228 ANDSER-574, AND MASS SPECTROMETRY.	19369195 [Abstract]
"Global survey of phosphotyrosine signaling identifies oncogenickinases in lung cancer."; Rikova K., Guo A., Zeng Q., Possemato A., Yu J., Haack H., Nardone J.,Lee K., Reeves C., Li Y., Hu Y., Tan Z., Stokes M., Sullivan L.,Mitchell J., Wetzel R., Macneill J., Ren J.M., Yuan J.,Bakalarski C.E., Villen J., Kornhauser J.M., Smith B., Li D., Zhou X.,Gygi S.P., Gu T.-L., Polakiewicz R.D., Rush J., Comb M.J.; Cell 131:1190-1203(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-1034 AND TYR-1035, ANDMASS SPECTROMETRY.	18083107 [Abstract]