HSPB8_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: HSPB8_HUMAN
• UniProt AC: Q9UJY1
• Protein Name: Heat shock protein beta-8
• Gene Name: HSPB8
• Organism: Homo sapiens (Human).
• Sequence Length: 196
• Subcellular Localization: Cytoplasm . Nucleus . Translocates to nuclear foci during heat shock.
• Protein Description: Displays temperature-dependent chaperone activity..
• Protein Sequence: MADGQMPFSCHYPSRLRRDPFRDSPLSSRLLDDGFGMDPFPDDLTASWPDWALPRLSSAWPGTLRSGMVPRGPTATARFGVPAEGRTPPPFPGEPWKVCVNVHSFKPEELMVKTKDGYVEVSGKHEEKQQEGGIVSKNFTKKIQLPAEVDPVTVFASLSPEGLLIIEAPQVPPYSTFGESSFNNELPQDSQEVTCT

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
9	Phosphorylation	ADGQMPFSCHYPSRL CCCCCCCCCCCCCHH	8.30	28857561
12	Phosphorylation	QMPFSCHYPSRLRRD CCCCCCCCCCHHCCC	13.77	24043423
14	Phosphorylation	PFSCHYPSRLRRDPF CCCCCCCCHHCCCCC	36.46	28857561
24	Phosphorylation	RRDPFRDSPLSSRLL CCCCCCCCCCHHHHC	24.39	22167270
27	Phosphorylation	PFRDSPLSSRLLDDG CCCCCCCHHHHCCCC	19.45	25867546
28	Phosphorylation	FRDSPLSSRLLDDGF CCCCCCHHHHCCCCC	34.33	22199227
57	Phosphorylation	DWALPRLSSAWPGTL CCHHHHHHCCCCCCC	21.20	29396449
58	Phosphorylation	WALPRLSSAWPGTLR CHHHHHHCCCCCCCC	38.66	29396449
63	O-linked_Glycosylation	LSSAWPGTLRSGMVP HHCCCCCCCCCCCCC	18.21	30379171
63	Phosphorylation	LSSAWPGTLRSGMVP HHCCCCCCCCCCCCC	18.21	11816564
66	O-linked_Glycosylation	AWPGTLRSGMVPRGP CCCCCCCCCCCCCCC	34.06	30379171
71	Methylation	LRSGMVPRGPTATAR CCCCCCCCCCCCCEE	52.92	26494661
71	Asymmetric dimethylarginine	LRSGMVPRGPTATAR CCCCCCCCCCCCCEE	52.92	-
78	Asymmetric dimethylarginine	RGPTATARFGVPAEG CCCCCCEECCCCCCC	25.21	-
78	Methylation	RGPTATARFGVPAEG CCCCCCEECCCCCCC	25.21	26494667
87	Phosphorylation	GVPAEGRTPPPFPGE CCCCCCCCCCCCCCC	51.51	25159151
104	Phosphorylation	KVCVNVHSFKPEELM EEEEEECCCCHHHHE	30.59	-
115	Ubiquitination	EELMVKTKDGYVEVS HHHEEEECCCEEEEC	43.36	29967540
118	Phosphorylation	MVKTKDGYVEVSGKH EEEECCCEEEECCCC	11.99	22817900
122	Phosphorylation	KDGYVEVSGKHEEKQ CCCEEEECCCCCHHH	28.72	-
124	Ubiquitination	GYVEVSGKHEEKQQE CEEEECCCCCHHHCC	40.09	22817900
128	Ubiquitination	VSGKHEEKQQEGGIV ECCCCCHHHCCCCEE	55.06	21963094
137	Ubiquitination	QEGGIVSKNFTKKIQ CCCCEECCCCCCCCC	45.23	29967540

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
14	S	Phosphorylation	Kinase	PRKCA	P05696	GPS
14	S	Phosphorylation	Kinase	PKC-FAMILY	-	GPS
14	S	Phosphorylation	Kinase	PKC_GROUP	-	PhosphoELM
24	S	Phosphorylation	Kinase	MAPK3	P27361	GPS
27	S	Phosphorylation	Kinase	MAPK3	P27361	GPS
57	S	Phosphorylation	Kinase	PRKACA	P17612	GPS
63	T	Phosphorylation	Kinase	PRKCA	P05696	GPS
63	T	Phosphorylation	Kinase	PKC-FAMILY	-	GPS
63	T	Phosphorylation	Kinase	PKC	-	Uniprot
63	T	Phosphorylation	Kinase	PKC_GROUP	-	PhosphoELM
87	T	Phosphorylation	Kinase	MAPK3	P27361	GPS

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of HSPB8_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of HSPB8_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
HSPB1_HUMAN	HSPB1	physical	14594798
HSPB7_HUMAN	HSPB7	physical	16189514
HSPB1_HUMAN	HSPB1	physical	15122253
HSPB8_HUMAN	HSPB8	physical	14594798
HSPB7_HUMAN	HSPB7	physical	14594798
HSPB2_HUMAN	HSPB2	physical	14594798
HSPB2_HUMAN	HSPB2	physical	11342557
HSPB6_HUMAN	HSPB6	physical	16225851
CRYAB_HUMAN	CRYAB	physical	16225851
HSPB3_HUMAN	HSPB3	physical	16225851
HSPB7_HUMAN	HSPB7	physical	25416956
QRIC1_HUMAN	QRICH1	physical	25416956
BAG3_HUMAN	BAG3	physical	21516116
LONF2_HUMAN	LONRF2	physical	28514442
NUD12_HUMAN	NUDT12	physical	28514442
BRCA2_HUMAN	BRCA2	physical	28514442
FA83H_HUMAN	FAM83H	physical	28514442
ACD11_HUMAN	ACAD11	physical	28514442
SETD2_HUMAN	SETD2	physical	28514442
BAG3_HUMAN	BAG3	physical	28514442
VWA8_HUMAN	VWA8	physical	28514442
ZNF92_HUMAN	ZNF92	physical	28514442
EF1A2_HUMAN	EEF1A2	physical	28514442
RFX1_HUMAN	RFX1	physical	28514442
ALMS1_HUMAN	ALMS1	physical	28514442
WDR20_HUMAN	WDR20	physical	28514442

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• 158590: Neuronopathy, distal hereditary motor, 2A (HMN2A)
• 608673: Charcot-Marie-Tooth disease 2L (CMT2L)
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"A quantitative atlas of mitotic phosphorylation.";
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.;
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-24, AND MASSSPECTROMETRY.
PubMed: 18669648

"Combining protein-based IMAC, peptide-based IMAC, and MudPIT forefficient phosphoproteomic analysis.";
Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D.,Yates J.R. III;
J. Proteome Res. 7:1346-1351(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-24, AND MASSSPECTROMETRY.
PubMed: 18220336

"Phosphopeptide derivatization signatures to identify serine andthreonine phosphorylated peptides by mass spectrometry.";
Molloy M.P., Andrews P.C.;
Anal. Chem. 73:5387-5394(2001).
Cited for: PHOSPHORYLATION AT THR-63, AND MASS SPECTROMETRY.
PubMed: 11816564

"Global survey of phosphotyrosine signaling identifies oncogenickinases in lung cancer.";
Rikova K., Guo A., Zeng Q., Possemato A., Yu J., Haack H., Nardone J.,Lee K., Reeves C., Li Y., Hu Y., Tan Z., Stokes M., Sullivan L.,Mitchell J., Wetzel R., Macneill J., Ren J.M., Yuan J.,Bakalarski C.E., Villen J., Kornhauser J.M., Smith B., Li D., Zhou X.,Gygi S.P., Gu T.-L., Polakiewicz R.D., Rush J., Comb M.J.;
Cell 131:1190-1203(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-118, AND MASSSPECTROMETRY.
PubMed: 18083107