dbPTM

GCH1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	GCH1_HUMAN
UniProt AC	P30793
Protein Name	GTP cyclohydrolase 1
Gene Name	GCH1
Organism	Homo sapiens (Human).
Sequence Length	250
Subcellular Localization	Cytoplasm . Nucleus .
Protein Description	Positively regulates nitric oxide synthesis in umbilical vein endothelial cells (HUVECs). May be involved in dopamine synthesis. May modify pain sensitivity and persistence. Isoform GCH-1 is the functional enzyme, the potential function of the enzymatically inactive isoforms remains unknown..
Protein Sequence	MEKGPVRAPAEKPRGARCSNGFPERDPPRPGPSRPAEKPPRPEAKSAQPADGWKGERPRSEEDNELNLPNLAAAYSSILSSLGENPQRQGLLKTPWRAASAMQFFTKGYQETISDVLNDAIFDEDHDEMVIVKDIDMFSMCEHHLVPFVGKVHIGYLPNKQVLGLSKLARIVEIYSRRLQVQERLTKQIAVAITEALRPAGVGVVVEATHMCMVMRGVQKMNSKTVTSTMLGVFREDPKTREEFLTLIRS

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
38	Ubiquitination	GPSRPAEKPPRPEAK CCCCCCCCCCCCCCC	63.53	-
54	Ubiquitination	AQPADGWKGERPRSE CCCCCCCCCCCCCCH	57.32	-
60	Phosphorylation	WKGERPRSEEDNELN CCCCCCCCHHCCCCC	48.28	28258704
60 (in isoform 2)	Phosphorylation	-	48.28	24719451
81	Phosphorylation	AYSSILSSLGENPQR HHHHHHHHCCCCHHH	36.63	11284739
93	Ubiquitination	PQRQGLLKTPWRAAS HHHCCCCCCHHHHHH	58.55	-
94	Phosphorylation	QRQGLLKTPWRAASA HHCCCCCCHHHHHHH	28.22	22210691
106	Phosphorylation	ASAMQFFTKGYQETI HHHHHHHHHHHHHHH	25.50	22985185
160	Ubiquitination	HIGYLPNKQVLGLSK EEEECCCCHHHCHHH	39.58	-
175	Phosphorylation	LARIVEIYSRRLQVQ HHHHHHHHHHHHHHH	5.21	-
176	Phosphorylation	ARIVEIYSRRLQVQE HHHHHHHHHHHHHHH	19.31	24719451
194 (in isoform 2)	Phosphorylation	-	12.88	24719451
210 (in isoform 2)	Phosphorylation	-	17.94	24719451
214 (in isoform 4)	Phosphorylation	-	1.69	-
223 (in isoform 4)	Phosphorylation	-	26.63	-
224 (in isoform 4)	Phosphorylation	-	41.85	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
81	S	Phosphorylation	Kinase	CK2A1	P68400	PSP

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
81	S	Phosphorylation		17704208
Phosphorylated by casein kinase II at Ser-81 in HAECs during oscillatory shear stress; phosphorylation at Ser-81 results in increased enzyme activity.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of GCH1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
GCH1_HUMAN	GCH1	physical	16696853
GFRP_HUMAN	GCHFR	physical	16696853
AHSA1_HUMAN	AHSA1	physical	16696853
RLF_HUMAN	RLF	physical	16696853
HSP7C_HUMAN	HSPA8	physical	16696853
SP3_HUMAN	SP3	physical	16696853
CNRP1_HUMAN	CNRIP1	physical	16696853
RAB26_HUMAN	RAB26	physical	16696853
AT1B1_HUMAN	ATP1B1	physical	16696853
PACN3_HUMAN	PACSIN3	physical	19294699
CALL5_HUMAN	CALML5	physical	19294699
CALL6_HUMAN	CALML6	physical	19294699
RGS19_HUMAN	RGS19	physical	19294699
PPM1B_HUMAN	PPM1B	physical	19294699
MP2K4_HUMAN	MAP2K4	physical	19294699
MP2K5_HUMAN	MAP2K5	physical	19294699
MK12_HUMAN	MAPK12	physical	19294699
MK10_HUMAN	MAPK10	physical	19294699
KKCC1_HUMAN	CAMKK1	physical	19294699
KC1G1_HUMAN	CSNK1G1	physical	19294699
CSK21_HUMAN	CSNK2A1	physical	19294699
CSK2B_HUMAN	CSNK2B	physical	19294699
KAP2_HUMAN	PRKAR2A	physical	19294699
KAP3_HUMAN	PRKAR2B	physical	19294699
GCH1_HUMAN	GCH1	physical	2463916
1433Z_HUMAN	YWHAZ	physical	21988832
GIT2_HUMAN	GIT2	physical	21988832
GCH1_HUMAN	GCH1	physical	11087827

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
233910	Hyperphenylalaninemia, BH4-deficient, B (HPABH4B)
128230	Dystonia, dopa-responsive (DRD)
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of GCH1_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions."; Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.; Sci. Signal. 2:RA46-RA46(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-60, AND MASSSPECTROMETRY.	19690332 [Abstract]
"Regulation of tetrahydrobiopterin biosynthesis by shear stress."; Widder J.D., Chen W., Li L., Dikalov S., Thony B., Hatakeyama K.,Harrison D.G.; Circ. Res. 101:830-838(2007). Cited for: PHOSPHORYLATION AT SER-81.	17704208 [Abstract]