MP2K5_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: MP2K5_HUMAN
• UniProt AC: Q13163
• Protein Name: Dual specificity mitogen-activated protein kinase kinase 5
• Gene Name: MAP2K5
• Organism: Homo sapiens (Human).
• Sequence Length: 448
• Protein Description: Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appears to play a critical role in protecting cells from stress-induced apoptosis, neuronal survival and cardiac development and angiogenesis..
• Protein Sequence: MLWLALGPFPAMENQVLVIRIKIPNSGAVDWTVHSGPQLLFRDVLDVIGQVLPEATTTAFEYEDEDGDRITVRSDEEMKAMLSYYYSTVMEQQVNGQLIEPLQIFPRACKPPGERNIHGLKVNTRAGPSQHSSPAVSDSLPSNSLKKSSAELKKILANGQMNEQDIRYRDTLGHGNGGTVYKAYHVPSGKILAVKVILLDITLELQKQIMSELEILYKCDSSYIIGFYGAFFVENRISICTEFMDGGSLDVYRKMPEHVLGRIAVAVVKGLTYLWSLKILHRDVKPSNMLVNTRGQVKLCDFGVSTQLVNSIAKTYVGTNAYMAPERISGEQYGIHSDVWSLGISFMELALGRFPYPQIQKNQGSLMPLQLLQCIVDEDSPVLPVGEFSEPFVHFITQCMRKQPKERPAPEELMGHPFIVQFNDGNAAVVSMWVCRALEERRSQQGPP

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
71	Phosphorylation	DEDGDRITVRSDEEM CCCCCEEEECCHHHH	15.68	-
121	Ubiquitination	ERNIHGLKVNTRAGP CCCCCCEEEECCCCC	37.72	-
125	Methylation	HGLKVNTRAGPSQHS CCEEEECCCCCCCCC	32.13	115483133
129	Phosphorylation	VNTRAGPSQHSSPAV EECCCCCCCCCCCCC	39.34	25159151
132	Phosphorylation	RAGPSQHSSPAVSDS CCCCCCCCCCCCCCC	30.31	21815630
133	Phosphorylation	AGPSQHSSPAVSDSL CCCCCCCCCCCCCCC	18.11	25159151
137	Phosphorylation	QHSSPAVSDSLPSNS CCCCCCCCCCCCCCC	24.48	29396449
142	Phosphorylation	AVSDSLPSNSLKKSS CCCCCCCCCCCCCCH	44.32	18691976
144	Phosphorylation	SDSLPSNSLKKSSAE CCCCCCCCCCCCHHH	46.00	25627689
146	Ubiquitination	SLPSNSLKKSSAELK CCCCCCCCCCHHHHH	50.89	-
148	Phosphorylation	PSNSLKKSSAELKKI CCCCCCCCHHHHHHH	32.94	18691976
149	Phosphorylation	SNSLKKSSAELKKIL CCCCCCCHHHHHHHH	34.34	22817900
154	Ubiquitination	KSSAELKKILANGQM CCHHHHHHHHHCCCC	56.42	-
202	Phosphorylation	KVILLDITLELQKQI EEHHHHHHHHHHHHH	17.53	26074081
211	Phosphorylation	ELQKQIMSELEILYK HHHHHHHHHHHHHHH	40.43	26074081
217	Phosphorylation	MSELEILYKCDSSYI HHHHHHHHHCCCCCE	18.41	26074081
298	Ubiquitination	VNTRGQVKLCDFGVS ECCCCCEEECCCCCC	35.19	-
305	Phosphorylation	KLCDFGVSTQLVNSI EECCCCCCHHHHHHH	15.71	28102081
306	Phosphorylation	LCDFGVSTQLVNSIA ECCCCCCHHHHHHHH	24.42	22210691
311	Phosphorylation	VSTQLVNSIAKTYVG CCHHHHHHHHHHCCC	19.22	25159151
315	Phosphorylation	LVNSIAKTYVGTNAY HHHHHHHHCCCCCCE	17.89	25159151
316	Phosphorylation	VNSIAKTYVGTNAYM HHHHHHHCCCCCCEE	9.00	19060867

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
129	S	Phosphorylation	Kinase	MAPK7	Q13164	GPS
137	S	Phosphorylation	Kinase	MAPK7	Q13164	GPS
142	S	Phosphorylation	Kinase	MAPK7	Q13164	GPS
149	S	Phosphorylation	Kinase	MAPK7	Q13164	GPS

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of MP2K5_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of MP2K5_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
M3K2_HUMAN	MAP3K2	physical	11073940
M3K3_HUMAN	MAP3K3	physical	11073940
KPCZ_HUMAN	PRKCZ	physical	11158308
MK07_HUMAN	MAPK7	physical	7759517
MP2K5_HUMAN	MAP2K5	physical	7759517
M3K3_MOUSE	Map3k3	physical	10593883
1433Z_HUMAN	YWHAZ	physical	20936779
MRCKB_HUMAN	CDC42BPB	physical	20936779
ENOA_HUMAN	ENO1	physical	15805119
SQSTM_HUMAN	SQSTM1	physical	19903815
M3K2_HUMAN	MAP3K2	physical	19903815
ASGL1_HUMAN	ASRGL1	physical	21900206
KPCI_HUMAN	PRKCI	physical	12813044
SQSTM_HUMAN	SQSTM1	physical	12813044
M3K3_HUMAN	MAP3K3	physical	12813044
PAR6A_HUMAN	PARD6A	physical	12813044
XRCC6_HUMAN	XRCC6	physical	21988832
RL28_HUMAN	RPL28	physical	21988832
M3K2_HUMAN	MAP3K2	physical	21988832
M3K2_HUMAN	MAP3K2	physical	24975362
MK07_HUMAN	MAPK7	physical	24975362
MK07_HUMAN	MAPK7	physical	28514442
M3K2_HUMAN	MAP3K2	physical	28514442
M3K3_HUMAN	MAP3K3	physical	28514442
TRAF7_HUMAN	TRAF7	physical	28514442
CJ088_HUMAN	C10orf88	physical	28514442
CDC37_HUMAN	CDC37	physical	28514442

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Large-scale proteomics analysis of the human kinome.";
Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G.,Mann M., Daub H.;
Mol. Cell. Proteomics 8:1751-1764(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-137; SER-148; SER-149;SER-311; THR-315 AND TYR-316, AND MASS SPECTROMETRY.
PubMed: 19369195

"Kinase-selective enrichment enables quantitative phosphoproteomics ofthe kinome across the cell cycle.";
Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R.,Greff Z., Keri G., Stemmann O., Mann M.;
Mol. Cell 31:438-448(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-133, AND MASSSPECTROMETRY.
PubMed: 18691976

"Components of a new human protein kinase signal transductionpathway.";
Zhou G., Bao Z.Q., Dixon J.E.;
J. Biol. Chem. 270:12665-12669(1995).
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, INTERACTION WITHERK5 AND MAPK7, MUTAGENESIS OF LYS-195; SER-311 AND THR-315, ANDPHOSPHORYLATION AT SER-311 AND THR-315.
PubMed: 7759517