dbPTM

MRCKB_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	MRCKB_HUMAN
UniProt AC	Q9Y5S2
Protein Name	Serine/threonine-protein kinase MRCK beta
Gene Name	CDC42BPB {ECO:0000312\|EMBL:AAD37506.1}
Organism	Homo sapiens (Human).
Sequence Length	1711
Subcellular Localization	Cytoplasm. Cell membrane Peripheral membrane protein Cytoplasmic side . Cell junction . Cell projection, lamellipodium . Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell
Protein Description	Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2. [PubMed: 21457715]
Protein Sequence	MSAKVRLKKLEQLLLDGPWRNESALSVETLLDVLVCLYTECSHSALRRDKYVAEFLEWAKPFTQLVKEMQLHREDFEIIKVIGRGAFGEVAVVKMKNTERIYAMKILNKWEMLKRAETACFREERDVLVNGDCQWITALHYAFQDENHLYLVMDYYVGGDLLTLLSKFEDKLPEDMARFYIGEMVLAIDSIHQLHYVHRDIKPDNVLLDVNGHIRLADFGSCLKMNDDGTVQSSVAVGTPDYISPEILQAMEDGMGKYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHEERFQFPSHVTDVSEEAKDLIQRLICSRERRLGQNGIEDFKKHAFFEGLNWENIRNLEAPYIPDVSSPSDTSNFDVDDDVLRNTEILPPGSHTGFSGLHLPFIGFTFTTESCFSDRGSLKSIMQSNTLTKDEDVQRDLEHSLQMEAYERRIRRLEQEKLELSRKLQESTQTVQSLHGSSRALSNSNRDKEIKKLNEEIERLKNKIADSNRLERQLEDTVALRQEREDSTQRLRGLEKQHRVVRQEKEELHKQLVEASERLKSQAKELKDAHQQRKLALQEFSELNERMAELRAQKQKVSRQLRDKEEEMEVATQKVDAMRQEMRRAEKLRKELEAQLDDAVAEASKERKLREHSENFCKQMESELEALKVKQGGRGAGATLEHQQEISKIKSELEKKVLFYEEELVRREASHVLEVKNVKKEVHDSESHQLALQKEILMLKDKLEKSKRERHNEMEEAVGTIKDKYERERAMLFDENKKLTAENEKLCSFVDKLTAQNRQLEDELQDLAAKKESVAHWEAQIAEIIQWVSDEKDARGYLQALASKMTEELEALRSSSLGSRTLDPLWKVRRSQKLDMSARLELQSALEAEIRAKQLVQEELRKVKDANLTLESKLKDSEAKNRELLEEMEILKKKMEEKFRADTGLKLPDFQDSIFEYFNTAPLAHDLTFRTSSASEQETQAPKPEASPSMSVAASEQQEDMARPPQRPSAVPLPTTQALALAGPKPKAHQFSIKSFSSPTQCSHCTSLMVGLIRQGYACEVCSFACHVSCKDGAPQVCPIPPEQSKRPLGVDVQRGIGTAYKGHVKVPKPTGVKKGWQRAYAVVCDCKLFLYDLPEGKSTQPGVIASQVLDLRDDEFSVSSVLASDVIHATRRDIPCIFRVTASLLGAPSKTSSLLILTENENEKRKWVGILEGLQSILHKNRLRNQVVHVPLEAYDSSLPLIKAILTAAIVDADRIAVGLEEGLYVIEVTRDVIVRAADCKKVHQIELAPREKIVILLCGRNHHVHLYPWSSLDGAEGSFDIKLPETKGCQLMATATLKRNSGTCLFVAVKRLILCYEIQRTKPFHRKFNEIVAPGSVQCLAVLRDRLCVGYPSGFCLLSIQGDGQPLNLVNPNDPSLAFLSQQSFDALCAVELESEEYLLCFSHMGLYVDPQGRRARAQELMWPAAPVACSCSPTHVTVYSEYGVDVFDVRTMEWVQTIGLRRIRPLNSEGTLNLLNCEPPRLIYFKSKFSGAVLNVPDTSDNSKKQMLRTRSKRRFVFKVPEEERLQQRREMLRDPELRSKMISNPTNFNHVAHMGPGDGMQVLMDLPLSAVPPSQEERPGPAPTNLARQPPSRNKPYISWPSSGGSEPSVTVPLRSMSDPDQDFDKEPDSDSTKHSTPSNSSNPSGPPSPNSPHRSQLPLEGLEQPACDT

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
166	Phosphorylation	GDLLTLLSKFEDKLP HHHHHHHHHHHHCCC	38.11	24719451
221	Phosphorylation	IRLADFGSCLKMNDD EEECCCCCCEEECCC	19.10	-
233	Phosphorylation	NDDGTVQSSVAVGTP CCCCCEEECEECCCC	23.57	-
239	Phosphorylation	QSSVAVGTPDYISPE EECEECCCCCCCCHH	13.34	11283256
244	Phosphorylation	VGTPDYISPEILQAM CCCCCCCCHHHHHHH	15.39	-
304	Phosphorylation	EERFQFPSHVTDVSE HHHCCCCCCCCCCCH	31.70	-
307	Phosphorylation	FQFPSHVTDVSEEAK CCCCCCCCCCCHHHH	25.84	-
310	Phosphorylation	PSHVTDVSEEAKDLI CCCCCCCCHHHHHHH	32.31	-
314	Ubiquitination	TDVSEEAKDLIQRLI CCCCHHHHHHHHHHH	57.24	21890473
337	Ubiquitination	QNGIEDFKKHAFFEG CCCHHHHHHHHHCCC	56.84	21890473
357	Phosphorylation	IRNLEAPYIPDVSSP HHCCCCCCCCCCCCC	31.94	30624053
362	Phosphorylation	APYIPDVSSPSDTSN CCCCCCCCCCCCCCC	43.86	26657352
363	Phosphorylation	PYIPDVSSPSDTSNF CCCCCCCCCCCCCCC	28.42	21082442
365	Phosphorylation	IPDVSSPSDTSNFDV CCCCCCCCCCCCCCC	56.31	30624053
367	Phosphorylation	DVSSPSDTSNFDVDD CCCCCCCCCCCCCCC	29.88	27732954
368	Phosphorylation	VSSPSDTSNFDVDDD CCCCCCCCCCCCCCH	40.25	27732954
417	Phosphorylation	SDRGSLKSIMQSNTL CCCCCHHHHHHHCCC	28.69	-
421	Phosphorylation	SLKSIMQSNTLTKDE CHHHHHHHCCCCCCH	17.88	28857561
423	Phosphorylation	KSIMQSNTLTKDEDV HHHHHHCCCCCCHHH	40.84	26657352
426	Ubiquitination	MQSNTLTKDEDVQRD HHHCCCCCCHHHHHH	63.33	21890473
454	Ubiquitination	IRRLEQEKLELSRKL HHHHHHHHHHHHHHH	47.13	-
460	Ubiquitination	EKLELSRKLQESTQT HHHHHHHHHHHHHHH	51.66	-
464	Phosphorylation	LSRKLQESTQTVQSL HHHHHHHHHHHHHHH	16.92	24275569
465	Phosphorylation	SRKLQESTQTVQSLH HHHHHHHHHHHHHHH	27.75	23312004
467	Phosphorylation	KLQESTQTVQSLHGS HHHHHHHHHHHHHHH	22.17	23312004
470	Phosphorylation	ESTQTVQSLHGSSRA HHHHHHHHHHHHHHH	20.28	24275569
474	Phosphorylation	TVQSLHGSSRALSNS HHHHHHHHHHHHHCC	13.10	24275569
475	Phosphorylation	VQSLHGSSRALSNSN HHHHHHHHHHHHCCC	26.60	28857561
479	Phosphorylation	HGSSRALSNSNRDKE HHHHHHHHCCCHHHH	36.91	26699800
481	Phosphorylation	SSRALSNSNRDKEIK HHHHHHCCCHHHHHH	29.84	27422710
489	Acetylation	NRDKEIKKLNEEIER CHHHHHHHHHHHHHH	63.31	20167786
524	Phosphorylation	LRQEREDSTQRLRGL HHHHCHHHHHHHHHH	22.99	-
571	Malonylation	KDAHQQRKLALQEFS HHHHHHHHHHHHHHH	33.50	26320211
571	Ubiquitination	KDAHQQRKLALQEFS HHHHHHHHHHHHHHH	33.50	-
605	Sulfoxidation	LRDKEEEMEVATQKV HHCHHHHHHHHHHHH	5.93	30846556
641	Phosphorylation	DDAVAEASKERKLRE HHHHHHHHHHHHHHH	26.93	-
642	Ubiquitination	DAVAEASKERKLREH HHHHHHHHHHHHHHH	69.46	-
659	Phosphorylation	NFCKQMESELEALKV HHHHHHHHHHHHHHC	41.98	-
665	Ubiquitination	ESELEALKVKQGGRG HHHHHHHHCCCCCCC	55.20	-
671	Methylation	LKVKQGGRGAGATLE HHCCCCCCCCCCCHH	38.32	24129315
676	Phosphorylation	GGRGAGATLEHQQEI CCCCCCCCHHHHHHH	31.59	25159151
697	Phosphorylation	LEKKVLFYEEELVRR HHHHHHHCHHHHHHH	20.47	27642862
707	Phosphorylation	ELVRREASHVLEVKN HHHHHHHHHHEEEEC	14.91	-
757	Phosphorylation	EMEEAVGTIKDKYER HHHHHHHHHHHHHHH	20.46	28060719
789	Ubiquitination	KLCSFVDKLTAQNRQ HHHHHHHHHHHHHHH	42.30	-
807	Ubiquitination	ELQDLAAKKESVAHW HHHHHHHHHHHHHHH	52.01	-
834	Phosphorylation	DEKDARGYLQALASK CCCCHHHHHHHHHHH	7.06	27174698
840	Phosphorylation	GYLQALASKMTEELE HHHHHHHHHHHHHHH	24.66	27174698
843	Phosphorylation	QALASKMTEELEALR HHHHHHHHHHHHHHH	30.00	19664994
851	Phosphorylation	EELEALRSSSLGSRT HHHHHHHHCCCCCCC	25.73	20068231
852	Phosphorylation	ELEALRSSSLGSRTL HHHHHHHCCCCCCCC	24.13	20068231
853	Phosphorylation	LEALRSSSLGSRTLD HHHHHHCCCCCCCCC	37.69	22210691
856	Phosphorylation	LRSSSLGSRTLDPLW HHHCCCCCCCCCHHH	27.64	23898821
858	Phosphorylation	SSSLGSRTLDPLWKV HCCCCCCCCCHHHHH	36.89	23403867
864	Ubiquitination	RTLDPLWKVRRSQKL CCCCHHHHHHHHCCC	33.28	21890473
864	Ubiquitination	RTLDPLWKVRRSQKL CCCCHHHHHHHHCCC	33.28	21890473
868	Phosphorylation	PLWKVRRSQKLDMSA HHHHHHHHCCCCHHH	22.54	24719451
870	Ubiquitination	WKVRRSQKLDMSARL HHHHHHCCCCHHHHH	47.93	-
881	Phosphorylation	SARLELQSALEAEIR HHHHHHHHHHHHHHH	47.66	-
890	Ubiquitination	LEAEIRAKQLVQEEL HHHHHHHHHHHHHHH	34.02	-
910	Ubiquitination	ANLTLESKLKDSEAK CCCCHHHHCCCCHHH	50.62	-
914	Phosphorylation	LESKLKDSEAKNREL HHHHCCCCHHHHHHH	37.56	22496350
929	Ubiquitination	LEEMEILKKKMEEKF HHHHHHHHHHHHHHH	56.88	-
954	Phosphorylation	FQDSIFEYFNTAPLA HHHHHHHHHHCCCCC	7.44	22817900
968	Phosphorylation	AHDLTFRTSSASEQE CCCCEEECCCCCCCC	23.89	23911959
969	Phosphorylation	HDLTFRTSSASEQET CCCEEECCCCCCCCC	21.46	29116813
970	Phosphorylation	DLTFRTSSASEQETQ CCEEECCCCCCCCCC	34.89	25627689
984	Phosphorylation	QAPKPEASPSMSVAA CCCCCCCCCCCCCHH	18.82	25627689
986	Phosphorylation	PKPEASPSMSVAASE CCCCCCCCCCCHHHH	22.61	25627689
1029	Phosphorylation	KPKAHQFSIKSFSSP CCCCEECEEECCCCC	23.65	20873877
1083	Ubiquitination	PIPPEQSKRPLGVDV CCCHHHCCCCCCCCC	58.70	-
1099	Ubiquitination	RGIGTAYKGHVKVPK CCCCCEECCCCCCCC	39.82	-
1118	Phosphorylation	KKGWQRAYAVVCDCK CCCHHHEEEEEECCE	11.34	28152594
1135	Ubiquitination	LYDLPEGKSTQPGVI EEECCCCCCCCCCEE	48.98	-
1179	Phosphorylation	IPCIFRVTASLLGAP CCEEEEEHHHHHCCC	13.28	28857561
1181	Phosphorylation	CIFRVTASLLGAPSK EEEEEHHHHHCCCCC	18.11	28857561
1188	Ubiquitination	SLLGAPSKTSSLLIL HHHCCCCCCCEEEEE	51.90	-
1189	Phosphorylation	LLGAPSKTSSLLILT HHCCCCCCCEEEEEE	28.08	23403867
1190	Phosphorylation	LGAPSKTSSLLILTE HCCCCCCCEEEEEEC	23.79	21406692
1191	Phosphorylation	GAPSKTSSLLILTEN CCCCCCCEEEEEECC	32.21	23403867
1196	Phosphorylation	TSSLLILTENENEKR CCEEEEEECCCCCCC	29.75	21406692
1202	Ubiquitination	LTENENEKRKWVGIL EECCCCCCCHHHHHH	71.50	-
1280	Ubiquitination	VRAADCKKVHQIELA EHHHHCCEEEEEEEC	51.81	-
1337	Methylation	LMATATLKRNSGTCL EEEEEEECCCCCCEE	45.22	115973247
1337	Ubiquitination	LMATATLKRNSGTCL EEEEEEECCCCCCEE	45.22	-
1355	Phosphorylation	VKRLILCYEIQRTKP EHHHHHHHHHHCCCC	16.78	-
1524	Phosphorylation	CEPPRLIYFKSKFSG CCCCEEEEEEECCCC	15.00	25599653
1526	Methylation	PPRLIYFKSKFSGAV CCEEEEEEECCCCCE	35.45	115973255
1527	Phosphorylation	PRLIYFKSKFSGAVL CEEEEEEECCCCCEE	29.09	25599653
1528	Methylation	RLIYFKSKFSGAVLN EEEEEEECCCCCEEC	44.11	54395553
1528	Ubiquitination	RLIYFKSKFSGAVLN EEEEEEECCCCCEEC	44.11	-
1530	Phosphorylation	IYFKSKFSGAVLNVP EEEEECCCCCEECCC	29.58	23911959
1544	Ubiquitination	PDTSDNSKKQMLRTR CCCCCCHHHHHHHHH	53.96	-
1550	Phosphorylation	SKKQMLRTRSKRRFV HHHHHHHHHHCCCCE	35.02	29514088
1552	Phosphorylation	KQMLRTRSKRRFVFK HHHHHHHHCCCCEEE	29.87	29514088
1559	Ubiquitination	SKRRFVFKVPEEERL HCCCCEEECCHHHHH	52.13	-
1638	Phosphorylation	PPSRNKPYISWPSSG CCCCCCCCEECCCCC	14.81	21945579
1640	Phosphorylation	SRNKPYISWPSSGGS CCCCCCEECCCCCCC	27.39	21945579
1643	Phosphorylation	KPYISWPSSGGSEPS CCCEECCCCCCCCCC	35.04	21945579
1644	Phosphorylation	PYISWPSSGGSEPSV CCEECCCCCCCCCCE	42.99	21945579
1647	Phosphorylation	SWPSSGGSEPSVTVP ECCCCCCCCCCEEEE	50.27	21945579
1650	Phosphorylation	SSGGSEPSVTVPLRS CCCCCCCCEEEECCC	26.77	21945579
1652	Phosphorylation	GGSEPSVTVPLRSMS CCCCCCEEEECCCCC	22.27	21945579
1657	Phosphorylation	SVTVPLRSMSDPDQD CEEEECCCCCCCCCC	30.26	23403867
1659	Phosphorylation	TVPLRSMSDPDQDFD EEECCCCCCCCCCCC	47.43	25159151
1671	Phosphorylation	DFDKEPDSDSTKHST CCCCCCCCCCCCCCC	44.14	23403867
1673	Phosphorylation	DKEPDSDSTKHSTPS CCCCCCCCCCCCCCC	43.22	23403867
1674	Phosphorylation	KEPDSDSTKHSTPSN CCCCCCCCCCCCCCC	37.45	23403867
1677	Phosphorylation	DSDSTKHSTPSNSSN CCCCCCCCCCCCCCC	43.50	23927012
1678	Phosphorylation	SDSTKHSTPSNSSNP CCCCCCCCCCCCCCC	30.38	23927012
1680	Phosphorylation	STKHSTPSNSSNPSG CCCCCCCCCCCCCCC	49.81	23927012
1682	Phosphorylation	KHSTPSNSSNPSGPP CCCCCCCCCCCCCCC	35.51	23927012
1683	Phosphorylation	HSTPSNSSNPSGPPS CCCCCCCCCCCCCCC	57.94	22167270
1686	Phosphorylation	PSNSSNPSGPPSPNS CCCCCCCCCCCCCCC	69.08	22167270
1690	Phosphorylation	SNPSGPPSPNSPHRS CCCCCCCCCCCCCCC	39.59	22167270
1693	Phosphorylation	SGPPSPNSPHRSQLP CCCCCCCCCCCCCCC	26.01	22167270
1697	Phosphorylation	SPNSPHRSQLPLEGL CCCCCCCCCCCCCCC	32.79	25159151
1711	Phosphorylation	LEQPACDT------- CCCCCCCC-------	40.15	29514088

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of MRCKB_HUMAN !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of MRCKB_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of MRCKB_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
RL3_HUMAN	RPL3	physical	17353931
DCD_HUMAN	DCD	physical	17353931
BRX1_HUMAN	BRIX1	physical	17353931

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of MRCKB_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Large-scale proteomics analysis of the human kinome."; Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G.,Mann M., Daub H.; Mol. Cell. Proteomics 8:1751-1764(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-362; SER-363; THR-676;SER-1686; SER-1690 AND SER-1693, AND MASS SPECTROMETRY.	19369195 [Abstract]
"Kinase-selective enrichment enables quantitative phosphoproteomics ofthe kinome across the cell cycle."; Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R.,Greff Z., Keri G., Stemmann O., Mann M.; Mol. Cell 31:438-448(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1690, AND MASSSPECTROMETRY.	18691976 [Abstract]
"Global, in vivo, and site-specific phosphorylation dynamics insignaling networks."; Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P.,Mann M.; Cell 127:635-648(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-914; SER-1690 ANDSER-1693, AND MASS SPECTROMETRY.	17081983 [Abstract]
"Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column."; Imami K., Sugiyama N., Kyono Y., Tomita M., Ishihama Y.; Anal. Sci. 24:161-166(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-1196, AND MASSSPECTROMETRY.	18187866 [Abstract]