dbPTM

M3K3_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	M3K3_MOUSE
UniProt AC	Q61084
Protein Name	Mitogen-activated protein kinase kinase kinase 3
Gene Name	Map3k3
Organism	Mus musculus (Mouse).
Sequence Length	626
Subcellular Localization
Protein Description	Component of a protein kinase signal transduction cascade. Mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators..
Protein Sequence	MDEQEALDSIMKDLVALQMSRRTRLSGYETMKNKDTGHPNRQSDVRIKFEHNGERRIIAFSRPVRYEDVEHKVTTVFGQPLDLHYMNNELSILLKNQDDLDKAIDILDRSSSMKSLRILLLSQDRNHTSSSPHSGVSRQVRIKPSQSAGDINTIYQAPEPRSRHLSVSSQNPGRSSPPPGYVPERQQHIARQGSYTSINSEGEFIPETSEQCMLDPLSSAENSLSGSCQSLDRSADSPSFRKSQMSRARSFPDNRKECSDRETQLYDKGVKGGTYPRRYHVSVHHKDYNDGRRTFPRIRRHQGNLFTLVPSSRSLSTNGENMGVAVQYLDPRGRLRSADSENALTVQERNVPTKSPSAPINWRRGKLLGQGAFGRVYLCYDVDTGRELASKQVQFDPDSPETSKEVSALECEIQLLKNLQHERIVQYYGCLRDRAEKILTIFMEYMPGGSVKDQLKAYGALTESVTRKYTRQILEGMSYLHSNMIVHRDIKGANILRDSAGNVKLGDFGASKRLQTICMSGTGIRSVTGTPYWMSPEVISGEGYGRKADVWSLGCTVVEMLTEKPPWAEYEAMAAIFKIATQPTNPQLPSHISEHGRDFLRRIFVEARQRPSAEELLTHHFAQLVY

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
20	Phosphorylation	DLVALQMSRRTRLSG HHHHHHHHHCCCCCC	12.97	29514104
23	Phosphorylation	ALQMSRRTRLSGYET HHHHHHCCCCCCHHH	34.55	29176673
26	Phosphorylation	MSRRTRLSGYETMKN HHHCCCCCCHHHHCC	35.72	29176673
122	Phosphorylation	SLRILLLSQDRNHTS HHHHEEECCCCCCCC	29.97	24719451
128	Phosphorylation	LSQDRNHTSSSPHSG ECCCCCCCCCCCCCC	33.58	27681418
129	Phosphorylation	SQDRNHTSSSPHSGV CCCCCCCCCCCCCCC	22.67	23684622
130	Phosphorylation	QDRNHTSSSPHSGVS CCCCCCCCCCCCCCC	49.39	29899451
131	Phosphorylation	DRNHTSSSPHSGVSR CCCCCCCCCCCCCCC	26.80	26824392
134	Phosphorylation	HTSSSPHSGVSRQVR CCCCCCCCCCCCCEE	44.25	29472430
137	Phosphorylation	SSPHSGVSRQVRIKP CCCCCCCCCCEEECC	22.11	28066266
145	Phosphorylation	RQVRIKPSQSAGDIN CCEEECCCCCCCCCC	31.59	27742792
147	Phosphorylation	VRIKPSQSAGDINTI EEECCCCCCCCCCEE	38.26	25521595
153	Phosphorylation	QSAGDINTIYQAPEP CCCCCCCEEEECCCC	22.77	25619855
155	Phosphorylation	AGDINTIYQAPEPRS CCCCCEEEECCCCCC	9.15	25619855
162	Phosphorylation	YQAPEPRSRHLSVSS EECCCCCCCCCCCCC	34.46	29514104
166	Phosphorylation	EPRSRHLSVSSQNPG CCCCCCCCCCCCCCC	17.52	25521595
168	Phosphorylation	RSRHLSVSSQNPGRS CCCCCCCCCCCCCCC	23.88	21082442
169	Phosphorylation	SRHLSVSSQNPGRSS CCCCCCCCCCCCCCC	31.64	25619855
175	Phosphorylation	SSQNPGRSSPPPGYV CCCCCCCCCCCCCCC	53.78	27742792
176	Phosphorylation	SQNPGRSSPPPGYVP CCCCCCCCCCCCCCC	39.81	27087446
181	Phosphorylation	RSSPPPGYVPERQQH CCCCCCCCCCHHHHH	20.71	25619855
234	Phosphorylation	SCQSLDRSADSPSFR CHHHCCCCCCCHHHH	35.77	27180971
237	Phosphorylation	SLDRSADSPSFRKSQ HCCCCCCCHHHHHHH	23.35	26824392
239	Phosphorylation	DRSADSPSFRKSQMS CCCCCCHHHHHHHHH	41.45	26060331
249	Methylation	KSQMSRARSFPDNRK HHHHHHHHCCCCCCH	37.41	16186231
250	Phosphorylation	SQMSRARSFPDNRKE HHHHHHHCCCCCCHH	39.90	26824392
255	Methylation	ARSFPDNRKECSDRE HHCCCCCCHHCCHHC	43.46	16186239
274	Phosphorylation	DKGVKGGTYPRRYHV HCCCCCCCCCCEEEE	38.73	23140645
294	Phosphorylation	DYNDGRRTFPRIRRH CCCCCCCCCCCCEEC	36.31	24719451
307	Phosphorylation	RHQGNLFTLVPSSRS ECCCCEEEECCCCCC	29.97	28285833
311	Phosphorylation	NLFTLVPSSRSLSTN CEEEECCCCCCCCCC	30.79	19367708
312	Phosphorylation	LFTLVPSSRSLSTNG EEEECCCCCCCCCCC	21.59	21454597
314	Phosphorylation	TLVPSSRSLSTNGEN EECCCCCCCCCCCCC	28.72	25266776
316	Phosphorylation	VPSSRSLSTNGENMG CCCCCCCCCCCCCCE	22.52	27180971
317	Phosphorylation	PSSRSLSTNGENMGV CCCCCCCCCCCCCEE	53.16	25266776
328	Phosphorylation	NMGVAVQYLDPRGRL CCEEEEEEECCCCCC	13.38	25777480
337	Phosphorylation	DPRGRLRSADSENAL CCCCCCCCCCCCCCC	40.81	27087446
340	Phosphorylation	GRLRSADSENALTVQ CCCCCCCCCCCCEEE	32.25	25521595
345	Phosphorylation	ADSENALTVQERNVP CCCCCCCEEECCCCC	20.10	25619855
353	Phosphorylation	VQERNVPTKSPSAPI EECCCCCCCCCCCCC	39.61	23140645
355	Phosphorylation	ERNVPTKSPSAPINW CCCCCCCCCCCCCCC	26.94	26824392
357	Phosphorylation	NVPTKSPSAPINWRR CCCCCCCCCCCCCCC	54.49	26060331
469	Phosphorylation	TESVTRKYTRQILEG CHHHHHHHHHHHHHH	12.12	25367039
470	Phosphorylation	ESVTRKYTRQILEGM HHHHHHHHHHHHHHH	21.09	25367039
482	Phosphorylation	EGMSYLHSNMIVHRD HHHHHHHCCCEECCC	26.04	25367039
511	Phosphorylation	KLGDFGASKRLQTIC ECCCCCCCCCCEEEE	20.77	29176673
526	Phosphorylation	MSGTGIRSVTGTPYW ECCCCEECCCCCCCE	23.31	16407301
528	Phosphorylation	GTGIRSVTGTPYWMS CCCEECCCCCCCEEC	36.26	30635358
530	Phosphorylation	GIRSVTGTPYWMSPE CEECCCCCCCEECCC	12.11	30635358
532	Phosphorylation	RSVTGTPYWMSPEVI ECCCCCCCEECCCCC	17.37	30635358
535	Phosphorylation	TGTPYWMSPEVISGE CCCCCEECCCCCCCC	12.08	30635358
540	Phosphorylation	WMSPEVISGEGYGRK EECCCCCCCCCCCCH	35.65	30635358
544	Phosphorylation	EVISGEGYGRKADVW CCCCCCCCCCHHHHH	14.95	30635358
552	Phosphorylation	GRKADVWSLGCTVVE CCHHHHHHHCCEEEH	18.09	20531401
556	Phosphorylation	DVWSLGCTVVEMLTE HHHHHCCEEEHHHHC	26.28	20531401
562	Phosphorylation	CTVVEMLTEKPPWAE CEEEHHHHCCCCHHH	40.42	20531401
570	Phosphorylation	EKPPWAEYEAMAAIF CCCCHHHHHHHHHHH	10.97	20531401

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
166	S	Phosphorylation	Kinase	SGK1	O00141	PSP
337	S	Phosphorylation	Kinase	SGK1	O00141	PSP
337	S	Phosphorylation	Kinase	SGK1	Q9WVC6	Uniprot
526	S	Phosphorylation	Kinase	MAP2K3	O09110	GPS

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Reference
166	S	Phosphorylation	21183079
Phosphorylation at Ser-166 and Ser-337 by SGK1 inhibits its activity.
337	S	Phosphorylation	17242355
Phosphorylation at Ser-166 and Ser-337 by SGK1 inhibits its activity.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of M3K3_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
MP2K5_HUMAN	MAP2K5	physical	10593883

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of M3K3_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Solid tumor proteome and phosphoproteome analysis by high resolutionmass spectrometry."; Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J.,Faessler R., Mann M.; J. Proteome Res. 7:5314-5326(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-166, AND MASSSPECTROMETRY.	19367708 [Abstract]
"Large-scale phosphorylation analysis of mouse liver."; Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-337 AND SER-340, ANDMASS SPECTROMETRY.	17242355 [Abstract]

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