DOCK8_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: DOCK8_HUMAN
• UniProt AC: Q8NF50
• Protein Name: Dedicator of cytokinesis protein 8
• Gene Name: DOCK8
• Organism: Homo sapiens (Human).
• Sequence Length: 2099
• Subcellular Localization: Cytoplasm . Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell projection, lamellipodium membrane ; Peripheral membrane protein ; Cytoplasmic side . Enriched and co-localizes with GTPase CDC42 at the immunological synapse formed du
• Protein Description: Guanine nucleotide exchange factor (GEF) which specifically activates small GTPase CDC42 by exchanging bound GDP for free GTP. [PubMed: 28028151]
• Protein Sequence: MATLPSAERRAFALKINRYSSAEIRKQFTLPPNLGQYHRQSISTSGFPSLQLPQFYDPVEPVDFEGLLMTHLNSLDVQLAQELGDFTDDDLDVVFTPKECRTLQPSLPEEGVELDPHVRDCVQTYIREWLIVNRKNQGSPEICGFKKTGSRKDFHKTLPKQTFESETLECSEPAAQAGPRHLNVLCDVSGKGPVTACDFDLRSLQPDKRLENLLQQVSAEDFEKQNEEARRTNRQAELFALYPSVDEEDAVEIRPVPECPKEHLGNRILVKLLTLKFEIEIEPLFASIALYDVKERKKISENFHCDLNSDQFKGFLRAHTPSVAASSQARSAVFSVTYPSSDIYLVVKIEKVLQQGEIGDCAEPYTVIKESDGGKSKEKIEKLKLQAESFCQRLGKYRMPFAWAPISLSSFFNVSTLEREVTDVDSVVGRSSVGERRTLAQSRRLSERALSLEENGVGSNFKTSTLSVSSFFKQEGDRLSDEDLFKFLADYKRSSSLQRRVKSIPGLLRLEISTAPEIINCCLTPEMLPVKPFPENRTRPHKEILEFPTREVYVPHTVYRNLLYVYPQRLNFVNKLASARNITIKIQFMCGEDASNAMPVIFGKSSGPEFLQEVYTAVTYHNKSPDFYEEVKIKLPAKLTVNHHLLFTFYHISCQQKQGASVETLLGYSWLPILLNERLQTGSYCLPVALEKLPPNYSMHSAEKVPLQNPPIKWAEGHKGVFNIEVQAVSSVHTQDNHLEKFFTLCHSLESQVTFPIRVLDQKISEMALEHELKLSIICLNSSRLEPLVLFLHLVLDKLFQLSVQPMVIAGQTANFSQFAFESVVAIANSLHNSKDLSKDQHGRNCLLASYVHYVFRLPEVQRDVPKSGAPTALLDPRSYHTYGRTSAAAVSSKLLQARVMSSSNPDLAGTHSAADEEVKNIMSSKIADRNCSRMSYYCSGSSDAPSSPAAPRPASKKHFHEELALQMVVSTGMVRETVFKYAWFFFELLVKSMAQHVHNMDKRDSFRRTRFSDRFMDDITTIVNVVTSEIAALLVKPQKENEQAEKMNISLAFFLYDLLSLMDRGFVFNLIRHYCSQLSAKLSNLPTLISMRLEFLRILCSHEHYLNLNLFFMNADTAPTSPCPSISSQNSSSCSSFQDQKIASMFDLTSEYRQQHFLTGLLFTELAAALDAEGEGISKVQRKAVSAIHSLLSSHDLDPRCVKPEVKVKIAALYLPLVGIILDALPQLCDFTVADTRRYRTSGSDEEQEGAGAINQNVALAIAGNNFNLKTSGIVLSSLPYKQYNMLNADTTRNLMICFLWIMKNADQSLIRKWIADLPSTQLNRILDLLFICVLCFEYKGKQSSDKVSTQVLQKSRDVKARLEEALLRGEGARGEMMRRRAPGNDRFPGLNENLRWKKEQTHWRQANEKLDKTKAELDQEALISGNLATEAHLIILDMQENIIQASSALDCKDSLLGGVLRVLVNSLNCDQSTTYLTHCFATLRALIAKFGDLLFEEEVEQCFDLCHQVLHHCSSSMDVTRSQACATLYLLMRFSFGATSNFARVKMQVTMSLASLVGRAPDFNEEHLRRSLRTILAYSEEDTAMQMTPFPTQVEELLCNLNSILYDTVKMREFQEDPEMLMDLMYRIAKSYQASPDLRLTWLQNMAEKHTKKKCYTEAAMCLVHAAALVAEYLSMLEDHSYLPVGSVSFQNISSNVLEESVVSEDTLSPDEDGVCAGQYFTESGLVGLLEQAAELFSTGGLYETVNEVYKLVIPILEAHREFRKLTLTHSKLQRAFDSIVNKDHKRMFGTYFRVGFFGSKFGDLDEQEFVYKEPAITKLPEISHRLEAFYGQCFGAEFVEVIKDSTPVDKTKLDPNKAYIQITFVEPYFDEYEMKDRVTYFEKNFNLRRFMYTTPFTLEGRPRGELHEQYRRNTVLTTMHAFPYIKTRISVIQKEEFVLTPIEVAIEDMKKKTLQLAVAINQEPPDAKMLQMVLQGSVGATVNQGPLEVAQVFLAEIPADPKLYRHHNKLRLCFKEFIMRCGEAVEKNKRLITADQREYQQELKKNYNKLKENLRPMIERKIPELYKPIFRVESQKRDSFHRSSFRKCETQLSQGS

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
3	Phosphorylation	-----MATLPSAERR -----CCCCCHHHHH	51.57	23401153
6	Phosphorylation	--MATLPSAERRAFA --CCCCCHHHHHHHH	45.96	29853039
19	Phosphorylation	FALKINRYSSAEIRK HHHHHCCCCCHHHHH	11.50	29978859
20	Phosphorylation	ALKINRYSSAEIRKQ HHHHCCCCCHHHHHH	21.60	28450419
21	Phosphorylation	LKINRYSSAEIRKQF HHHCCCCCHHHHHHC	22.46	28450419
29	Phosphorylation	AEIRKQFTLPPNLGQ HHHHHHCCCCCCCCH	35.54	28450419
139	Phosphorylation	VNRKNQGSPEICGFK HCCCCCCCCCCCCEE	15.03	23401153
146	Ubiquitination	SPEICGFKKTGSRKD CCCCCCEECCCCCHH	34.41	-
148	Phosphorylation	EICGFKKTGSRKDFH CCCCEECCCCCHHHH	40.77	24719451
157	Phosphorylation	SRKDFHKTLPKQTFE CCHHHHHHCCCCCCC	39.46	24719451
232	Phosphorylation	QNEEARRTNRQAELF HHHHHHHHHHHHHHH	29.24	24719451
242	Phosphorylation	QAELFALYPSVDEED HHHHHHHCCCCCHHH	7.05	24719451
244	Phosphorylation	ELFALYPSVDEEDAV HHHHHCCCCCHHHCC	28.75	27080861
365	Phosphorylation	IGDCAEPYTVIKESD CCCCCCCEEEEECCC	12.73	-
366	Phosphorylation	GDCAEPYTVIKESDG CCCCCCEEEEECCCC	26.86	-
409	Phosphorylation	AWAPISLSSFFNVST CCCCEEHHHCCCHHH	20.50	28348404
410	Phosphorylation	WAPISLSSFFNVSTL CCCEEHHHCCCHHHC	38.87	28348404
415	Phosphorylation	LSSFFNVSTLEREVT HHHCCCHHHCEEEEC	28.51	28348404
416	Phosphorylation	SSFFNVSTLEREVTD HHCCCHHHCEEEECC	28.49	28348404
422	Phosphorylation	STLEREVTDVDSVVG HHCEEEECCHHHHCC	26.08	28857561
426	Phosphorylation	REVTDVDSVVGRSSV EEECCHHHHCCCCCH	20.67	28857561
438	Phosphorylation	SSVGERRTLAQSRRL CCHHHHHHHHHHHHH	32.44	20068231
442	Phosphorylation	ERRTLAQSRRLSERA HHHHHHHHHHHHHHH	17.48	20068231
446	Phosphorylation	LAQSRRLSERALSLE HHHHHHHHHHHHHHH	24.19	20068231
451	Phosphorylation	RLSERALSLEENGVG HHHHHHHHHHHCCCC	32.52	23401153
459	Phosphorylation	LEENGVGSNFKTSTL HHHCCCCCCCEECEE	36.37	27690223
494	Phosphorylation	FLADYKRSSSLQRRV HHHHHHCCHHHHHHH	21.81	21964256
495	Phosphorylation	LADYKRSSSLQRRVK HHHHHCCHHHHHHHH	38.82	21964256
559	Phosphorylation	VYVPHTVYRNLLYVY EECCHHHHHHHHHHC	8.57	-
615	Phosphorylation	PEFLQEVYTAVTYHN HHHHHHHHHHHHCCC	6.55	-
648	Phosphorylation	VNHHLLFTFYHISCQ ECEEEEEEEEEECCC	24.21	-
713	Ubiquitination	PLQNPPIKWAEGHKG CCCCCCCCCCCCCCC	46.23	21890473
713	Ubiquitination	PLQNPPIKWAEGHKG CCCCCCCCCCCCCCC	46.23	21890473
713 (in isoform 1)	Ubiquitination	-	46.23	21890473
713 (in isoform 2)	Ubiquitination	-	46.23	21890473
879	Phosphorylation	TALLDPRSYHTYGRT CCCCCCCCCCCCCCH	27.01	27080861
882	Phosphorylation	LDPRSYHTYGRTSAA CCCCCCCCCCCHHHH	21.31	27251275
883	Phosphorylation	DPRSYHTYGRTSAAA CCCCCCCCCCHHHHH	7.41	27251275
887	Phosphorylation	YHTYGRTSAAAVSSK CCCCCCHHHHHHHHH	17.98	24719451
894	Ubiquitination	SAAAVSSKLLQARVM HHHHHHHHHHHHHHH	45.79	21890473
894	Ubiquitination	SAAAVSSKLLQARVM HHHHHHHHHHHHHHH	45.79	21890473
894 (in isoform 1)	Ubiquitination	-	45.79	21890473
894 (in isoform 2)	Ubiquitination	-	45.79	21890473
902	Phosphorylation	LLQARVMSSSNPDLA HHHHHHHCCCCCCCC	27.97	23401153
903	Phosphorylation	LQARVMSSSNPDLAG HHHHHHCCCCCCCCC	18.95	23401153
904	Phosphorylation	QARVMSSSNPDLAGT HHHHHCCCCCCCCCC	45.11	23401153
911	Phosphorylation	SNPDLAGTHSAADEE CCCCCCCCCCCCHHH	13.67	23927012
913	Phosphorylation	PDLAGTHSAADEEVK CCCCCCCCCCHHHHH	25.62	20873877
926	Ubiquitination	VKNIMSSKIADRNCS HHHHHCCHHCCCCCC	34.31	-
933	Phosphorylation	KIADRNCSRMSYYCS HHCCCCCCCCCEECC	34.27	-
936	Phosphorylation	DRNCSRMSYYCSGSS CCCCCCCCEECCCCC	16.31	23401153
937	Phosphorylation	RNCSRMSYYCSGSSD CCCCCCCEECCCCCC	10.11	29978859
938	Phosphorylation	NCSRMSYYCSGSSDA CCCCCCEECCCCCCC	3.47	23186163
940	O-linked_Glycosylation	SRMSYYCSGSSDAPS CCCCEECCCCCCCCC	26.11	27655845
940	Phosphorylation	SRMSYYCSGSSDAPS CCCCEECCCCCCCCC	26.11	30108239
942	Phosphorylation	MSYYCSGSSDAPSSP CCEECCCCCCCCCCC	14.57	23186163
943	Phosphorylation	SYYCSGSSDAPSSPA CEECCCCCCCCCCCC	41.12	23186163
947	Phosphorylation	SGSSDAPSSPAAPRP CCCCCCCCCCCCCCC	51.15	25849741
948	Phosphorylation	GSSDAPSSPAAPRPA CCCCCCCCCCCCCCC	19.94	23401153
956	O-linked_Glycosylation	PAAPRPASKKHFHEE CCCCCCCCHHCCCHH	44.94	27655845
956	Phosphorylation	PAAPRPASKKHFHEE CCCCCCCCHHCCCHH	44.94	30108239
957	Acetylation	AAPRPASKKHFHEEL CCCCCCCHHCCCHHH	52.99	7708435
993	Phosphorylation	FFELLVKSMAQHVHN HHHHHHHHHHHHHHC	16.33	-
1075	Phosphorylation	VFNLIRHYCSQLSAK HHHHHHHHHHHHHHH	5.50	29978859
1077	Phosphorylation	NLIRHYCSQLSAKLS HHHHHHHHHHHHHHC	27.30	29978859
1080	Phosphorylation	RHYCSQLSAKLSNLP HHHHHHHHHHHCCCC	18.73	29978859
1084	Phosphorylation	SQLSAKLSNLPTLIS HHHHHHHCCCCHHHH	35.30	29978859
1088	Phosphorylation	AKLSNLPTLISMRLE HHHCCCCHHHHHHHH	39.63	29978859
1091	Phosphorylation	SNLPTLISMRLEFLR CCCCHHHHHHHHHHH	10.85	22210691
1145	Phosphorylation	FQDQKIASMFDLTSE HHHHHHHHHHCCCHH	24.37	28450419
1240	Phosphorylation	TVADTRRYRTSGSDE EEECCCCCCCCCCHH	18.23	28450419
1242	Phosphorylation	ADTRRYRTSGSDEEQ ECCCCCCCCCCHHHH	28.62	28192239
1243	Phosphorylation	DTRRYRTSGSDEEQE CCCCCCCCCCHHHHC	27.57	26657352
1245	Phosphorylation	RRYRTSGSDEEQEGA CCCCCCCCHHHHCCC	41.43	26657352
1272	Phosphorylation	GNNFNLKTSGIVLSS CCCCCCCCCCEEEEC	35.04	28857561
1273	Phosphorylation	NNFNLKTSGIVLSSL CCCCCCCCCEEEECC	25.57	28857561
1278	Phosphorylation	KTSGIVLSSLPYKQY CCCCEEEECCCHHHH	20.95	30108239
1279	Phosphorylation	TSGIVLSSLPYKQYN CCCEEEECCCHHHHC	29.24	30108239
1282	Phosphorylation	IVLSSLPYKQYNMLN EEEECCCHHHHCCCC	18.93	30108239
1310	Phosphorylation	IMKNADQSLIRKWIA HHHCCCHHHHHHHHC	26.72	24719451
1340	Phosphorylation	ICVLCFEYKGKQSSD HHHHHHHHCCCCCCC	12.56	-
1350	Phosphorylation	KQSSDKVSTQVLQKS CCCCCHHHHHHHHHC	20.89	23917254
1351	Phosphorylation	QSSDKVSTQVLQKSR CCCCHHHHHHHHHCH	26.19	-
1414	Acetylation	QANEKLDKTKAELDQ HHHHHHHHHHHHHCH	63.46	25953088
1524	Phosphorylation	SSMDVTRSQACATLY CCCCCCHHHHHHHHH	16.86	-
1628	Phosphorylation	EMLMDLMYRIAKSYQ HHHHHHHHHHHHHHC	13.23	24275569
1696	Phosphorylation	SVSFQNISSNVLEES CEEEEECCCCCCEEC	24.33	22468782
1697	Phosphorylation	VSFQNISSNVLEESV EEEEECCCCCCEECC	27.45	22468782
1793	Phosphorylation	DHKRMFGTYFRVGFF HHHHHHCCEEEEEEC	14.28	25137130
1848	Phosphorylation	FVEVIKDSTPVDKTK HHHHHCCCCCCCCCC	29.61	26074081
1849	Phosphorylation	VEVIKDSTPVDKTKL HHHHCCCCCCCCCCC	37.15	26074081
1854	Phosphorylation	DSTPVDKTKLDPNKA CCCCCCCCCCCCCCC	32.16	26074081
1862	Phosphorylation	KLDPNKAYIQITFVE CCCCCCCEEEEEEEE	8.58	26074081
1866	Phosphorylation	NKAYIQITFVEPYFD CCCEEEEEEEECCCC	12.78	26074081
1882	Phosphorylation	YEMKDRVTYFEKNFN CCCCCCEEEEHHHCC	24.32	-
1883	Phosphorylation	EMKDRVTYFEKNFNL CCCCCEEEEHHHCCE	13.59	-
1895	Phosphorylation	FNLRRFMYTTPFTLE CCEEEEEEECCCCCC	12.54	18083107
1897	Ubiquitination	LRRFMYTTPFTLEGR EEEEEEECCCCCCCC	10.02	21890473
1897 (in isoform 2)	Ubiquitination	-	10.02	21890473
1929	Ubiquitination	MHAFPYIKTRISVIQ HHHHHHHHHHEEEEE	26.38	21890473
1929 (in isoform 1)	Ubiquitination	-	26.38	21890473
1956	Phosphorylation	IEDMKKKTLQLAVAI HHHHHHCHHHHHHHH	28.72	-
2036	Phosphorylation	EKNKRLITADQREYQ HHCCCCCCCCHHHHH	28.86	27794612
2050	Phosphorylation	QQELKKNYNKLKENL HHHHHHHHHHHHHHH	23.66	18083107
2069	Phosphorylation	ERKIPELYKPIFRVE HHHCHHHHHCCCEEC	17.04	29978859
2077	Phosphorylation	KPIFRVESQKRDSFH HCCCEECCCCCCCCC	37.84	23401153
2082	Phosphorylation	VESQKRDSFHRSSFR ECCCCCCCCCHHHHH	27.30	23401153
2086	Phosphorylation	KRDSFHRSSFRKCET CCCCCCHHHHHHHHH	25.84	23401153
2087	Phosphorylation	RDSFHRSSFRKCETQ CCCCCHHHHHHHHHH	29.09	19776401
2093	Phosphorylation	SSFRKCETQLSQGS- HHHHHHHHHHHCCC-	44.26	28450419
2096	Phosphorylation	RKCETQLSQGS---- HHHHHHHHCCC----	23.76	25159151
2099	Phosphorylation	ETQLSQGS------- HHHHHCCC-------	29.43	28450419

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
2077	S	Phosphorylation	Kinase	PRKCA	P17252	GPS
2082	S	Phosphorylation	Kinase	PRKCA	P17252	GPS
2087	S	Phosphorylation	Kinase	PRKCA	P17252	GPS

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of DOCK8_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of DOCK8_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
CDC42_HUMAN	CDC42	physical	15304341
RAC1_HUMAN	RAC1	physical	15304341
CRLF3_HUMAN	CRLF3	physical	24255178
DOCK6_HUMAN	DOCK6	physical	24255178
DOCK7_HUMAN	DOCK7	physical	24255178
LRCH1_HUMAN	LRCH1	physical	24255178
LRCH2_HUMAN	LRCH2	physical	24255178
LRCH3_HUMAN	LRCH3	physical	24255178
DOCK8_HUMAN	DOCK8	physical	25416956
F161A_HUMAN	FAM161A	physical	25416956
LRCH3_HUMAN	LRCH3	physical	25416956
MED30_HUMAN	MED30	physical	25416956
MTG1_HUMAN	MTG1	physical	25416956
K1C40_HUMAN	KRT40	physical	25416956
ZC3H1_HUMAN	ZFC3H1	physical	25416956
F124A_HUMAN	FAM124A	physical	25416956
RTP5_HUMAN	RTP5	physical	25416956
LRCH1_HUMAN	LRCH1	physical	28514442
LRCH2_HUMAN	LRCH2	physical	28514442
LRCH4_HUMAN	LRCH4	physical	28514442
DOCK7_HUMAN	DOCK7	physical	28514442
DOCK6_HUMAN	DOCK6	physical	28514442
APBP2_HUMAN	APPBP2	physical	28514442
CPNE7_HUMAN	CPNE7	physical	28514442

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• 243700: Hyperimmunoglobulin E recurrent infection syndrome, autosomal recessive (AR-HIES)
• 614113: Mental retardation, autosomal dominant 2 (MRD2)
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Large-scale proteomics analysis of the human kinome.";
Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G.,Mann M., Daub H.;
Mol. Cell. Proteomics 8:1751-1764(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-451, AND MASSSPECTROMETRY.
PubMed: 19369195