RUM1_SCHPO - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: RUM1_SCHPO
• UniProt AC: P40380
• Protein Name: Cyclin-dependent kinase inhibitor rum1
• Gene Name: rum1
• Organism: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
• Sequence Length: 230
• Subcellular Localization: Nucleus .
• Protein Description: Regulator of cell cycle G1 phase progression. Ensures the correct sequence of S phase and mitosis in the cell by acting as an inhibitor of the cdc2 mitotic kinase. Probably interacts with cdc2 to inhibit its action until the cell mass for Start is reached. Determines the length of the pre-Start G1 period and prevents mitosis from happening in early G1 cells. Required for maintaining pheromone-induced G1 arrest. Acts as an adapter protein since interaction with cdc13 promotes cyclin proteolysis during G1. Becomes a target for degradation at the G1/S phase transition, following phosphorylation by cig1-associated cdc2 at the G1/S phase transition..
• Protein Sequence: MEPSTPPMRGLCTPSTPESPGSFKGVIDASLEGNSSIMIDEIPESDLPAPQVSTFPPTPAKTPKKQLLPNLMLQDRSNSLERCMEEDREHNPFLSSSDNQLLSRKKRKPTPPPSDGLYYVFRGKRIKKSFRPGTDLSTFKPKLLFADSAPSSSSDNPTSSVDLNDYSQIGILPPNLNSIGNKMFSLKSRVPSSSSGSFVAPPPQMRLPAYSSPQKSRSNTKDENRHNLLR

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
13	Phosphorylation	PPMRGLCTPSTPESP CCCCCCCCCCCCCCC	25.89	28889911
16	Phosphorylation	RGLCTPSTPESPGSF CCCCCCCCCCCCCCC	32.07	25720772
19	Phosphorylation	CTPSTPESPGSFKGV CCCCCCCCCCCCCCE	35.45	28889911
58	Phosphorylation	QVSTFPPTPAKTPKK CCCCCCCCCCCCCHH	35.80	28889911
62	Phosphorylation	FPPTPAKTPKKQLLP CCCCCCCCCHHHHCC	42.31	28889911
211	Phosphorylation	QMRLPAYSSPQKSRS CCCCCCCCCCCCCCC	37.33	29996109
212	Phosphorylation	MRLPAYSSPQKSRSN CCCCCCCCCCCCCCC	20.76	29996109

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
13	T	Phosphorylation	Kinase	MAPK-FAMILY	-	GPS
13	T	Phosphorylation	Kinase	MAPK	-	Uniprot
19	S	Phosphorylation	Kinase	MAPK-FAMILY	-	GPS
19	S	Phosphorylation	Kinase	MAPK	-	Uniprot
58	T	Phosphorylation	Kinase	CDK1	P04551	GPS
62	T	Phosphorylation	Kinase	CDK1	P04551	GPS
-	K	Ubiquitination	E3 ubiquitin ligase	pop2#pop1	O14170#P87060	PMID:22199232

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of RUM1_SCHPO !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of RUM1_SCHPO !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
CG23_SCHPO	cdc13	physical	9614176
CG22_SCHPO	cig2	physical	9614176
POP1_SCHPO	pop1	physical	12167173
POP2_SCHPO	pop2	physical	12167173
CG23_SCHPO	cdc13	physical	9303310
CDK1_SCHPO	cdc2	physical	9303310
UBI4P_SCHPO	ubi4	physical	9203581
SWR1_SCHPO	swr1	genetic	9571240
SRW1_SCHPO	srw1	genetic	9398669
CDC18_SCHPO	cdc18	genetic	10388806
DNLI1_SCHPO	cdc17	genetic	10388806
RAD4_SCHPO	rad4	genetic	10388806
DPOD2_SCHPO	cdc1	genetic	10388806
DPOA_SCHPO	pol1	genetic	10388806
CDC24_SCHPO	cdc24	genetic	10388806
MCM10_SCHPO	cdc23	genetic	10388806
DPOE_SCHPO	cdc20	genetic	10388806
ORC1_SCHPO	orc1	genetic	10388806
DPOD_SCHPO	cdc6	genetic	10388806
CDC10_SCHPO	cdc10	genetic	10388806
MCM2_SCHPO	mcm2	genetic	10388806

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Rum1, an inhibitor of cyclin-dependent kinase in fission yeast, isnegatively regulated by mitogen-activated protein kinase-mediatedphosphorylation at Ser and Thr residues.";
Matsuoka K., Kiyokawa N., Taguchi T., Matsui J., Suzuki T., Mimori K.,Nakajima H., Takenouchi H., Weiran T., Katagiri Y.U., Fujimoto J.;
Eur. J. Biochem. 269:3511-3521(2002).
Cited for: FUNCTION, PHOSPHORYLATION AT THR-13 AND SER-19, AND MUTAGENESIS OFTHR-5; THR-13; THR-16; SER-19 AND THR-58.
PubMed: 12135491

"Regulation of the G1 phase of the cell cycle by periodicstabilization and degradation of the p25rum1 CDK inhibitor.";
Benito J., Martin-Castellanos C., Moreno S.;
EMBO J. 17:482-497(1998).
Cited for: FUNCTION, PHOSPHORYLATION AT THR-58 AND THR-62, AND MUTAGENESIS OFTHR-5; THR-13; THR-16; SER-19; THR-58; THR-62; THR-110 AND SER-212.
PubMed: 9430640