SHRPN_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: SHRPN_MOUSE
• UniProt AC: Q91WA6
• Protein Name: Sharpin
• Gene Name: Sharpin
• Organism: Mus musculus (Mouse).
• Sequence Length: 380
• Subcellular Localization: Cytoplasm, cytosol. Cell junction, synapse. Enriched at synaptic sites in mature neurons where it colocalizes with SHANK1..
• Protein Description: Component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with FAM105B/otulin, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis..
• Protein Sequence: MSPPAGGAAVAADPASPVVLLAVHAAVRPLGAGQDAEAQPRKLQLIADPERPGRFRLGLLGTEPGAVSLEWPLEAICYTVRGPNQHELQPPPGGPGTFSVHFLDPEEAQQWAALVRDATAEGQNGSGSPAPAPAPAMCPISPPCSSMAQIPKATQPEVDLPQSSGNFKKEELATRLSQAIAGGDEKAAAQVAAVLAQHHVALNVQLMEAWFPPGPIRLQVTVEDATSVLSSSSSAHVSLKIHPHCSIAALQDQVFSEFGFPPAVQRWVIGRCLCMPERSLASYGVSQDGDPAFLYLLSAPREVSGQSLQNSKMDRKLGLFPQSLGLPHDLQPSSSSLPSPSQPGWSCPSCTFINASNRPGCEMCSTQRPCAWDPLAAAST

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
32	Ubiquitination	AAVRPLGAGQDAEAQ HHHCCCCCCCCCCCC	21.84	27667366
42	Ubiquitination	DAEAQPRKLQLIADP CCCCCCCEEEEEECC	47.47	-
141	Phosphorylation	APAMCPISPPCSSMA CCCCCCCCCCCCHHH	14.13	28285833
163	Phosphorylation	PEVDLPQSSGNFKKE CCCCCCCCCCCCCHH	37.97	-
168	Ubiquitination	PQSSGNFKKEELATR CCCCCCCCHHHHHHH	64.37	27667366
169	Ubiquitination	QSSGNFKKEELATRL CCCCCCCHHHHHHHH	52.89	-
174	Phosphorylation	FKKEELATRLSQAIA CCHHHHHHHHHHHHH	44.78	25338131
176	Ubiquitination	KEELATRLSQAIAGG HHHHHHHHHHHHHCC	3.75	27667366
186	Ubiquitination	AIAGGDEKAAAQVAA HHHCCCHHHHHHHHH	48.33	-
260	Ubiquitination	QVFSEFGFPPAVQRW HHHHHHCCCHHHHHH	8.76	27667366
307	Phosphorylation	PREVSGQSLQNSKMD CCCCCCCHHHCCHHH	35.13	30352176
312	Ubiquitination	GQSLQNSKMDRKLGL CCHHHCCHHHHHCCC	52.98	22790023

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of SHRPN_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of SHRPN_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of SHRPN_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
EYA1_MOUSE	Eya1	physical	20956555
HOIL1_MOUSE	Rbck1	physical	21455181
RNF31_MOUSE	Rnf31	physical	21455181
FYB1_MOUSE	Fyb	physical	21829440
SCNBA_MOUSE	Scn11a	physical	21829440
ZN106_MOUSE	Zfp106	physical	21829440
STK24_MOUSE	Stk24	physical	21829440
CUX1_MOUSE	Cux1	physical	21829440
TRAF2_MOUSE	Traf2	physical	21829440
TEKT4_MOUSE	Tekt4	physical	21829440
ASAP2_MOUSE	Asap2	physical	21829440
CEBPA_MOUSE	Cebpa	physical	21829440
PSMD1_MOUSE	Psmd1	physical	21829440
PACA_MOUSE	Adcyap1	physical	21829440
BRE1A_MOUSE	Rnf20	physical	21829440
AIFM1_MOUSE	Aifm1	physical	21829440
UCHL1_MOUSE	Uchl1	physical	21829440
SURF6_MOUSE	Surf6	physical	21829440
CAN13_MOUSE	Capn13	physical	21829440

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.