TRAF2_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: TRAF2_MOUSE
• UniProt AC: P39429
• Protein Name: TNF receptor-associated factor 2
• Gene Name: Traf2
• Organism: Mus musculus (Mouse).
• Sequence Length: 501
• Subcellular Localization: Cytoplasm.
• Protein Description: Regulates activation of NF-kappa-B and JNK and plays a central role in the regulation of cell survival and apoptosis. Required for normal antibody isotype switching from IgM to IgG. Has E3 ubiquitin-protein ligase activity and promotes 'Lys-63'-linked ubiquitination of target proteins, such as BIRC3, RIPK1 and TICAM1. Is an essential constituent of several E3 ubiquitin-protein ligase complexes, where it promotes the ubiquitination of target proteins by bringing them into contact with other E3 ubiquitin ligases. Regulates BIRC2 and BIRC3 protein levels by inhibiting their autoubiquitination and subsequent degradation; this does not depend on the TRAF2 RING-type zinc finger domain. Isoform 2 does not seem to mediate activation of NF-kappa-B but inhibits isoform 1 activity. Plays a role in mediating activation of NF-kappa-B by EIF2AK2/PKR. In complex with BIRC2 or BIRC3, promotes ubiquitination of IKBKE..
• Protein Sequence: MAAASVTSPGSLELLQPGFSKTLLGTRLEAKYLCSACKNILRRPFQAQCGHRYCSFCLTSILSSGPQNCAACVYEGLYEEGISILESSSAFPDNAARREVESLPAVCPNDGCTWKGTLKEYESCHEGLCPFLLTECPACKGLVRLSEKEHHTEQECPKRSLSCQHCRAPCSHVDLEVHYEVCPKFPLTCDGCGKKKIPRETFQDHVRACSKCRVLCRFHTVGCSEMVETENLQDHELQRLREHLALLLSSFLEAQASPGTLNQVGPELLQRCQILEQKIATFENIVCVLNREVERVAVTAEACSRQHRLDQDKIEALSNKVQQLERSIGLKDLAMADLEQKVSELEVSTYDGVFIWKISDFTRKRQEAVAGRTPAIFSPAFYTSRYGYKMCLRVYLNGDGTGRGTHLSLFFVVMKGPNDALLQWPFNQKVTLMLLDHNNREHVIDAFRPDVTSSSFQRPVSDMNIASGCPLFCPVSKMEAKNSYVRDDAIFIKAIVDLTGL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MAAASVTSP ------CCCCCCCCC	16.25	-
5	Phosphorylation	---MAAASVTSPGSL ---CCCCCCCCCCCH	22.62	25266776
7	Phosphorylation	-MAAASVTSPGSLEL -CCCCCCCCCCCHHH	27.06	25266776
8	Phosphorylation	MAAASVTSPGSLELL CCCCCCCCCCCHHHC	25.71	27566939
11	Phosphorylation	ASVTSPGSLELLQPG CCCCCCCCHHHCCCC	23.27	26824392
20	Phosphorylation	ELLQPGFSKTLLGTR HHCCCCCCCHHCCHH	30.55	23984901
21	Ubiquitination	LLQPGFSKTLLGTRL HCCCCCCCHHCCHHH	40.71	-
22	Phosphorylation	LQPGFSKTLLGTRLE CCCCCCCHHCCHHHH	26.47	-
31	Ubiquitination	LGTRLEAKYLCSACK CCHHHHHHHHHHHHH	29.40	-
38	Ubiquitination	KYLCSACKNILRRPF HHHHHHHHHHHHCHH	46.90	-
117	Phosphorylation	DGCTWKGTLKEYESC CCCCCCCCHHHHHHH	30.81	11909853

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
117	T	Phosphorylation	Kinase	PRKCA	P17252	GPS
117	T	Phosphorylation	Kinase	PKC	-	Uniprot
-	K	Ubiquitination	E3 ubiquitin ligase	Siah2	Q06986	PMID:22199232

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
117	T	Phosphorylation	Phosphorylated at Thr-117 in response to signaling via TNF and TNFRSF1A.	19150425
117	T	Phosphorylation	Phosphorylation at Thr-117 is important for interaction with IKKA and IKKB, activation of IKK and subsequent activation of NF-kappa-B.	19150425
117	T	Phosphorylation	Phosphorylation at Thr-117 is required for 'Lys-63'-linked polyubiquitination, but not for 'Lys-48'-linked polyubiquitination.	19150425
117	T	Phosphorylation	Polyubiquitinated via 'Lys-63'-linked ubiquitin in response to TNF signaling; this requires prior phosphorylation at Thr-117.	19150425
117	T	ubiquitylation	Phosphorylation at Thr-117 is required for 'Lys-63'-linked polyubiquitination, but not for 'Lys-48'-linked polyubiquitination.	19150425
117	T	ubiquitylation	Polyubiquitinated via 'Lys-63'-linked ubiquitin in response to TNF signaling; this requires prior phosphorylation at Thr-117.	19150425

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of TRAF2_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
RBCC1_MOUSE	Rb1cc1	physical	17015619
M3K7_MOUSE	Map3k7	physical	22057290
UBE2N_MOUSE	Ube2n	physical	20185725
TNAP3_MOUSE	Tnfaip3	physical	20185725
UB2D3_MOUSE	Ube2d3	physical	20185725
ITCH_MOUSE	Itch	physical	20185725
RNF11_MOUSE	Rnf11	physical	20185725
TAXB1_MOUSE	Tax1bp1	physical	20185725
BIRC3_MOUSE	Birc3	physical	21765415
ITCH_MOUSE	Itch	physical	21765415
TNAP3_MOUSE	Tnfaip3	physical	21765415
RIPK1_MOUSE	Ripk1	physical	21765415
TAXB1_MOUSE	Tax1bp1	physical	21765415
UBE2N_MOUSE	Ube2n	physical	21765415
CDK9_MOUSE	Cdk9	physical	9827693
M3K5_MOUSE	Map3k5	physical	17015619
FHL2_MOUSE	Fhl2	physical	16184196
TIFA_MOUSE	Tifa	physical	11798190
MAVS_MOUSE	Mavs	physical	23951545
CREB1_MOUSE	Creb1	physical	26755589

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.