dbPTM

NFL_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	NFL_HUMAN
UniProt AC	P07196
Protein Name	Neurofilament light polypeptide
Gene Name	NEFL
Organism	Homo sapiens (Human).
Sequence Length	543
Subcellular Localization
Protein Description	Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber..
Protein Sequence	MSSFSYEPYYSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSAPVSSSLSVRRSYSSSSGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAEDATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMDEISFLKKVHEEEIAELQAQIQYAQISVEMDVTKPDLSAALKDIRAQYEKLAAKNMQNAEEWFKSRFTVLTESAAKNTDAVRAAKDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADISAMQDTINKLENELRTTKSEMARYLKEYQDLLNVKMALDIEIAAYRKLLEGEETRLSFTSVGSITSGYSQSSQVFGRSAYGGLQTSSYLMSTRSFPSYYTSHVQEEQIEVEETIEAAKAEEAKDEPPSEGEAEEEEKDKEEAEEEEAAEEEEAAKEESEEAKEEEEGGEGEEGEETKEAEEEEKKVEGAGEEQAAKKKD

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MSSFSYEPY ------CCCCCCCCC	39.84	22223895
2	Phosphorylation	------MSSFSYEPY ------CCCCCCCCC	39.84	28857561
3	Phosphorylation	-----MSSFSYEPYY -----CCCCCCCCCC	19.53	28348404
11	Phosphorylation	FSYEPYYSTSYKRRY CCCCCCCCCCCCCCC	12.67	22912867
13	Phosphorylation	YEPYYSTSYKRRYVE CCCCCCCCCCCCCEE	23.79	28857561
14	Phosphorylation	EPYYSTSYKRRYVET CCCCCCCCCCCCEEC	14.49	-
15	Ubiquitination	PYYSTSYKRRYVETP CCCCCCCCCCCEECC	30.11	21890473
15	Ubiquitination	PYYSTSYKRRYVETP CCCCCCCCCCCEECC	30.11	21890473
18	Phosphorylation	STSYKRRYVETPRVH CCCCCCCCEECCCEE	13.48	26074081
21	O-linked_Glycosylation	YKRRYVETPRVHISS CCCCCEECCCEEHHH	13.32	UniProtKB CARBOHYD
21	O-linked_Glycosylation	YKRRYVETPRVHISS CCCCCEECCCEEHHH	13.32	8344946
21	Phosphorylation	YKRRYVETPRVHISS CCCCCEECCCEEHHH	13.32	20860994
23	Asymmetric dimethylarginine	RRYVETPRVHISSVR CCCEECCCEEHHHCC	39.81	-
23	Methylation	RRYVETPRVHISSVR CCCEECCCEEHHHCC	39.81	58858675
27	O-linked_Glycosylation	ETPRVHISSVRSGYS ECCCEEHHHCCCCCC	14.11	UniProtKB CARBOHYD
27	Phosphorylation	ETPRVHISSVRSGYS ECCCEEHHHCCCCCC	14.11	26074081
28	Phosphorylation	TPRVHISSVRSGYST CCCEEHHHCCCCCCC	22.43	26074081
30	Methylation	RVHISSVRSGYSTAR CEEHHHCCCCCCCHH	26.72	58858687
31	Phosphorylation	VHISSVRSGYSTARS EEHHHCCCCCCCHHH	39.67	26074081
33	Phosphorylation	ISSVRSGYSTARSAY HHHCCCCCCCHHHHH	12.23	26074081
34	O-linked_Glycosylation	SSVRSGYSTARSAYS HHCCCCCCCHHHHHH	21.08	-
34	O-linked_Glycosylation	SSVRSGYSTARSAYS HHCCCCCCCHHHHHH	21.08	8702840
34	Phosphorylation	SSVRSGYSTARSAYS HHCCCCCCCHHHHHH	21.08	26074081
35	Phosphorylation	SVRSGYSTARSAYSS HCCCCCCCHHHHHHC	20.01	26074081
37	Methylation	RSGYSTARSAYSSYS CCCCCCHHHHHHCCC	23.16	97770653
38	O-linked_Glycosylation	SGYSTARSAYSSYSA CCCCCHHHHHHCCCC	29.15	30379171
38	Phosphorylation	SGYSTARSAYSSYSA CCCCCHHHHHHCCCC	29.15	28857561
40	Phosphorylation	YSTARSAYSSYSAPV CCCHHHHHHCCCCCC	10.21	24076635
41	O-linked_Glycosylation	STARSAYSSYSAPVS CCHHHHHHCCCCCCC	23.63	30379171
41	Phosphorylation	STARSAYSSYSAPVS CCHHHHHHCCCCCCC	23.63	28857561
42	O-linked_Glycosylation	TARSAYSSYSAPVSS CHHHHHHCCCCCCCC	15.49	30379171
42	Phosphorylation	TARSAYSSYSAPVSS CHHHHHHCCCCCCCC	15.49	27732954
43	Phosphorylation	ARSAYSSYSAPVSSS HHHHHHCCCCCCCCC	11.69	24076635
44	O-linked_Glycosylation	RSAYSSYSAPVSSSL HHHHHCCCCCCCCCC	28.22	30379171
44	Phosphorylation	RSAYSSYSAPVSSSL HHHHHCCCCCCCCCC	28.22	28857561
48	O-linked_Glycosylation	SSYSAPVSSSLSVRR HCCCCCCCCCCEEEC	17.10	23301498
48	O-linked_Glycosylation	SSYSAPVSSSLSVRR HCCCCCCCCCCEEEC	17.10	8702840
48	Phosphorylation	SSYSAPVSSSLSVRR HCCCCCCCCCCEEEC	17.10	24076635
49	O-linked_Glycosylation	SYSAPVSSSLSVRRS CCCCCCCCCCEEECC	35.22	30379171
49	Phosphorylation	SYSAPVSSSLSVRRS CCCCCCCCCCEEECC	35.22	27732954
50	O-linked_Glycosylation	YSAPVSSSLSVRRSY CCCCCCCCCEEECCC	19.70	30379171
50	Phosphorylation	YSAPVSSSLSVRRSY CCCCCCCCCEEECCC	19.70	28857561
52	O-linked_Glycosylation	APVSSSLSVRRSYSS CCCCCCCEEECCCCC	18.61	30379171
52	Phosphorylation	APVSSSLSVRRSYSS CCCCCCCEEECCCCC	18.61	28857561
56	Phosphorylation	SSLSVRRSYSSSSGS CCCEEECCCCCCCCC	20.85	25884760
57	Phosphorylation	SLSVRRSYSSSSGSL CCEEECCCCCCCCCC	15.68	28857561
58	Phosphorylation	LSVRRSYSSSSGSLM CEEECCCCCCCCCCC	25.62	28857561
59	Phosphorylation	SVRRSYSSSSGSLMP EEECCCCCCCCCCCC	21.93	28857561
60	Phosphorylation	VRRSYSSSSGSLMPS EECCCCCCCCCCCCC	32.55	28857561
61	Phosphorylation	RRSYSSSSGSLMPSL ECCCCCCCCCCCCCC	34.06	28857561
63	Phosphorylation	SYSSSSGSLMPSLEN CCCCCCCCCCCCCCC	24.36	28857561
67	Phosphorylation	SSGSLMPSLENLDLS CCCCCCCCCCCCCHH	34.06	28857561
74	Phosphorylation	SLENLDLSQVAAISN CCCCCCHHHHHHHHH	23.44	27732954
91	Ubiquitination	KSIRTQEKAQLQDLN HHHHHHHHHHHHHHH	31.45	30230243
103	Phosphorylation	DLNDRFASFIERVHE HHHHHHHHHHHHHHH	24.84	25332170
154	Phosphorylation	RLAAEDATNEKQALQ HHHHHHCCCHHHHHH	57.12	20071362
157	Ubiquitination	AEDATNEKQALQGER HHHCCCHHHHHHHHH	42.83	30230243
184	Phosphorylation	RYEEEVLSREDAEGR HHHHHHHCHHHHHHH	38.51	25307156
215	Phosphorylation	ELEKRIDSLMDEISF HHHHHHHHHHHHHHH	24.19	19678766
221	Phosphorylation	DSLMDEISFLKKVHE HHHHHHHHHHHHHCH	23.60	24076635
225	Ubiquitination	DEISFLKKVHEEEIA HHHHHHHHHCHHHHH	51.47	30230243
267	Ubiquitination	DIRAQYEKLAAKNMQ HHHHHHHHHHHHHCC	38.19	22817900
271	Ubiquitination	QYEKLAAKNMQNAEE HHHHHHHHHCCCHHH	47.55	21890473
271	Ubiquitination	QYEKLAAKNMQNAEE HHHHHHHHHCCCHHH	47.55	22817900
285	Phosphorylation	EWFKSRFTVLTESAA HHHHHHHHHHCHHHH	17.31	28509920
288	Phosphorylation	KSRFTVLTESAAKNT HHHHHHHCHHHHCCC	24.45	28509920
290	Phosphorylation	RFTVLTESAAKNTDA HHHHHCHHHHCCCHH	28.43	28509920
293	Ubiquitination	VLTESAAKNTDAVRA HHCHHHHCCCHHHHH	61.16	32142685
315	Ubiquitination	SRRLLKAKTLEIEAC HHHHHHHHHHHHHHH	52.64	30230243
345	Phosphorylation	DKQNADISAMQDTIN HHHHCCHHHHHHHHH	20.45	25307156
360	O-linked_Glycosylation	KLENELRTTKSEMAR HHHHHHHCCHHHHHH	51.72	30379171
360	Phosphorylation	KLENELRTTKSEMAR HHHHHHHCCHHHHHH	51.72	23312004
361	Phosphorylation	LENELRTTKSEMARY HHHHHHCCHHHHHHH	26.58	23312004
362	Acetylation	ENELRTTKSEMARYL HHHHHCCHHHHHHHH	43.75	7479679
363	Phosphorylation	NELRTTKSEMARYLK HHHHCCHHHHHHHHH	30.43	23312004
370	Ubiquitination	SEMARYLKEYQDLLN HHHHHHHHHHHHHHC	45.50	21890473
370	Ubiquitination	SEMARYLKEYQDLLN HHHHHHHHHHHHHHC	45.50	22817900
372	Phosphorylation	MARYLKEYQDLLNVK HHHHHHHHHHHHCHH	13.02	-
379	Acetylation	YQDLLNVKMALDIEI HHHHHCHHHHHHHHH	19.86	7479695
389	Phosphorylation	LDIEIAAYRKLLEGE HHHHHHHHHHHHCCC	10.16	-
398	Phosphorylation	KLLEGEETRLSFTSV HHHCCCCCEEEEEEC	32.38	21815630
403	O-linked_Glycosylation	EETRLSFTSVGSITS CCCEEEEEECCCCCC	21.08	30379171
404	O-linked_Glycosylation	ETRLSFTSVGSITSG CCEEEEEECCCCCCC	23.59	30379171
407	O-linked_Glycosylation	LSFTSVGSITSGYSQ EEEEECCCCCCCCCC	22.12	30379171
407	Phosphorylation	LSFTSVGSITSGYSQ EEEEECCCCCCCCCC	22.12	24076635
409	O-linked_Glycosylation	FTSVGSITSGYSQSS EEECCCCCCCCCCCC	20.14	30379171
413	O-linked_Glycosylation	GSITSGYSQSSQVFG CCCCCCCCCCCCCCC	27.41	30379171
415	O-linked_Glycosylation	ITSGYSQSSQVFGRS CCCCCCCCCCCCCCC	19.46	30379171
416	O-linked_Glycosylation	TSGYSQSSQVFGRSA CCCCCCCCCCCCCCC	23.79	30379171
422	O-linked_Glycosylation	SSQVFGRSAYGGLQT CCCCCCCCCCCCCCC	26.65	30379171
424	Phosphorylation	QVFGRSAYGGLQTSS CCCCCCCCCCCCCHH	16.99	24076635
429	O-linked_Glycosylation	SAYGGLQTSSYLMST CCCCCCCCHHHHHCC	25.36	30379171
429	Phosphorylation	SAYGGLQTSSYLMST CCCCCCCCHHHHHCC	25.36	24076635
430	O-linked_Glycosylation	AYGGLQTSSYLMSTR CCCCCCCHHHHHCCC	12.20	30379171
430	Phosphorylation	AYGGLQTSSYLMSTR CCCCCCCHHHHHCCC	12.20	24076635
431	O-linked_Glycosylation	YGGLQTSSYLMSTRS CCCCCCHHHHHCCCC	26.18	30379171
431	Phosphorylation	YGGLQTSSYLMSTRS CCCCCCHHHHHCCCC	26.18	24076635
432	Phosphorylation	GGLQTSSYLMSTRSF CCCCCHHHHHCCCCC	13.24	24076635
435	Phosphorylation	QTSSYLMSTRSFPSY CCHHHHHCCCCCCCH	20.34	24076635
436	O-linked_Glycosylation	TSSYLMSTRSFPSYY CHHHHHCCCCCCCHH	19.16	30379171
472	Phosphorylation	EAKDEPPSEGEAEEE HHCCCCCCCCCHHHH	69.79	26503892
502	Phosphorylation	EEAAKEESEEAKEEE HHHHHHHHHHHHHHH	41.66	25332170
520	Phosphorylation	EGEEGEETKEAEEEE CCCCCCHHHHHHHHH	29.86	20860994

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
21	T	Phosphorylation	Kinase	CDK5	Q00535	PSP
21	T	Phosphorylation	Kinase	MAPK1	P28482	GPS
-	K	Ubiquitination	E3 ubiquitin ligase	TRIM2	Q9C040	PMID:18687884

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of NFL_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of NFL_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
PKN1_HUMAN	PKN1	physical	8621664
TSC1_HUMAN	TSC1	physical	12226091
MTAP2_HUMAN	MAP2	physical	1902666
PIAS4_HUMAN	PIAS4	physical	15383276
VIME_HUMAN	VIM	physical	15383276
BRD7_HUMAN	BRD7	physical	15383276
KDM1A_HUMAN	KDM1A	physical	23455924
ANM6_HUMAN	PRMT6	physical	23455924
UBP2_HUMAN	USP2	physical	25416956
MYOME_HUMAN	PDE4DIP	physical	25416956
PP16B_HUMAN	PPP1R16B	physical	25416956
NDK7_HUMAN	NME7	physical	25416956
TEX11_HUMAN	TEX11	physical	25416956
ENKD1_HUMAN	ENKD1	physical	25416956
TXLNB_HUMAN	TXLNB	physical	25416956
PPR18_HUMAN	PPP1R18	physical	25416956
A4_HUMAN	APP	physical	25241761
BICD2_HUMAN	BICD2	physical	28514442
BICD1_HUMAN	BICD1	physical	28514442
DPP9_HUMAN	DPP9	physical	28514442
MIC1_HUMAN	C18orf8	physical	28514442
FA83H_HUMAN	FAM83H	physical	28514442
RABE1_HUMAN	RABEP1	physical	28514442
SC16A_HUMAN	SEC16A	physical	28514442

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
607734	Charcot-Marie-Tooth disease 1F (CMT1F)
607684	Charcot-Marie-Tooth disease 2E (CMT2E)
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of NFL_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Global proteomic profiling of phosphopeptides using electron transferdissociation tandem mass spectrometry."; Molina H., Horn D.M., Tang N., Mathivanan S., Pandey A.; Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-472, AND MASSSPECTROMETRY.	17287340 [Abstract]