DPH1_YEAST - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: DPH1_YEAST
• UniProt AC: P40487
• Protein Name: 2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 {ECO:0000305}
• Gene Name: DPH1
• Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
• Sequence Length: 425
• Subcellular Localization: Cytoplasm .
• Protein Description: Required for the first step of diphthamide biosynthesis, the transfer of 3-amino-3-carboxypropyl from S-adenosyl-L-methionine to a histidine residue. Diphthamide is a post-translational modification of histidine which occurs in elongation factor 2..
• Protein Sequence: MSGSTESKKQPRRRFIGRKSGNSNNDKLTTVAENGNEIIHKQKSRIALGRSVNHVPEDILNDKELNEAIKLLPSNYNFEIHKTVWNIRKYNAKRIALQMPEGLLIYSLIISDILEQFCGVETLVMGDVSYGACCIDDFTARALDCDFIVHYAHSCLVPIDVTKIKVLYVFVTINIQEDHIIKTLQKNFPKGSRIATFGTIQFNPAVHSVRDKLLNDEEHMLYIIPPQIKPLSRGEVLGCTSERLDKEQYDAMVFIGDGRFHLESAMIHNPEIPAFKYDPYNRKFTREGYDQKQLVEVRAEAIEVARKGKVFGLILGALGRQGNLNTVKNLEKNLIAAGKTVVKIILSEVFPQKLAMFDQIDVFVQVACPRLSIDWGYAFNKPLLTPYEASVLLKKDVMFSEKYYPMDYYEAKGYGRGETPKHAIE

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
44	Phosphorylation	EIIHKQKSRIALGRS CEEHCCHHHHCCCCC	26.82	17287358
51	Phosphorylation	SRIALGRSVNHVPED HHHCCCCCCCCCCHH	26.02	24961812
63	Acetylation	PEDILNDKELNEAIK CHHHCCHHHHHHHHH	64.13	24489116
289	Phosphorylation	RKFTREGYDQKQLVE CCCCCCCCCHHHHEE	15.50	27017623
332	Acetylation	NTVKNLEKNLIAAGK HHHHHHHHHHHHCCH	61.29	24489116
340	Phosphorylation	NLIAAGKTVVKIILS HHHHCCHHHHHHHHH	29.61	19795423
403	Phosphorylation	DVMFSEKYYPMDYYE CCCCCCCCCCCCHHC	14.56	27017623
404	Phosphorylation	VMFSEKYYPMDYYEA CCCCCCCCCCCHHCC	11.80	27017623
408	Phosphorylation	EKYYPMDYYEAKGYG CCCCCCCHHCCCCCC	9.28	27017623

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of DPH1_YEAST !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of DPH1_YEAST !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of DPH1_YEAST !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
IPYR_YEAST	IPP1	physical	16554755
MNN1_YEAST	MNN1	physical	16554755
IF4A_YEAST	TIF2	physical	16554755
ELM1_YEAST	ELM1	physical	16554755
DPH2_YEAST	DPH2	physical	16554755
TRX1_YEAST	TRX1	physical	16554755
STM1_YEAST	STM1	physical	16554755
CORO_YEAST	CRN1	physical	16554755
ADH3_YEAST	ADH3	physical	16554755
HSC82_YEAST	HSC82	physical	16554755
VDAC1_YEAST	POR1	physical	16554755
ZEO1_YEAST	ZEO1	physical	16554755
DPH2_YEAST	DPH2	physical	16429126
RIR2_YEAST	RNR2	physical	16429126
DPH2_YEAST	DPH2	physical	11283351
DPH2_YEAST	DPH2	physical	23645155
DPH5_YEAST	DPH5	genetic	23645155
DPH2_YEAST	DPH2	physical	24422557
SWI6_YEAST	SWI6	genetic	27708008
PAT1_YEAST	PAT1	genetic	27708008
EF2_YEAST	EFT2	genetic	27708008
AK_YEAST	HOM3	genetic	27708008
SNF6_YEAST	SNF6	genetic	27708008
VPS53_YEAST	VPS53	genetic	27708008
SRC1_YEAST	SRC1	genetic	27708008
BUL2_YEAST	BUL2	genetic	27708008
DPH2_HUMAN	DPH2	physical	27107014

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Analysis of phosphorylation sites on proteins from Saccharomycescerevisiae by electron transfer dissociation (ETD) massspectrometry.";
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L.,Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.;
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-44, AND MASSSPECTROMETRY.
PubMed: 17287358