dbPTM

STF1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	STF1_HUMAN
UniProt AC	Q13285
Protein Name	Steroidogenic factor 1
Gene Name	NR5A1
Organism	Homo sapiens (Human).
Sequence Length	461
Subcellular Localization	Nucleus .
Protein Description	Transcriptional activator. Essential for sexual differentiation and formation of the primary steroidogenic tissues. [PubMed: 27378692 Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1]
Protein Sequence	MDYSYDEDLDELCPVCGDKVSGYHYGLLTCESCKGFFKRTVQNNKHYTCTESQSCKIDKTQRKRCPFCRFQKCLTVGMRLEAVRADRMRGGRNKFGPMYKRDRALKQQKKAQIRANGFKLETGPPMGVPPPPPPAPDYVLPPSLHGPEPKGLAAGPPAGPLGDFGAPALPMAVPGAHGPLAGYLYPAFPGRAIKSEYPEPYASPPQPGLPYGYPEPFSGGPNVPELILQLLQLEPDEDQVRARILGCLQEPTKSRPDQPAAFGLLCRMADQTFISIVDWARRCMVFKELEVADQMTLLQNCWSELLVFDHIYRQVQHGKEGSILLVTGQEVELTTVATQAGSLLHSLVLRAQELVLQLLALQLDRQEFVCLKFIILFSLDLKFLNNHILVKDAQEKANAALLDYTLCHYPHCGDKFQQLLLCLVEVRALSMQAKEYLYHKHLGNEMPRNNLLIEMLQAKQT

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
34	Acetylation	LLTCESCKGFFKRTV EEEHHHCCCHHHHHH	68.95	11479297
38	Acetylation	ESCKGFFKRTVQNNK HHCCCHHHHHHCCCC	44.92	11479297
72	Acetylation	CPFCRFQKCLTVGMR CCCHHHHHHHHHHHH	28.62	11479297
100	"N6,N6-dimethyllysine"	NKFGPMYKRDRALKQ CCCCHHHHHHHHHHH	41.38	-
100	Methylation	NKFGPMYKRDRALKQ CCCCHHHHHHHHHHH	41.38	-
106	2-Hydroxyisobutyrylation	YKRDRALKQQKKAQI HHHHHHHHHHHHHHH	50.09	-
119	Sumoylation	QIRANGFKLETGPPM HHHHCCEECCCCCCC	46.97	-
119	Ubiquitination	QIRANGFKLETGPPM HHHHCCEECCCCCCC	46.97	1999590
119	Sumoylation	QIRANGFKLETGPPM HHHHCCEECCCCCCC	46.97	15192080
194	Sumoylation	AFPGRAIKSEYPEPY CCCCCCCCCCCCCCC	35.93	-
194	Sumoylation	AFPGRAIKSEYPEPY CCCCCCCCCCCCCCC	35.93	15192080
203	Phosphorylation	EYPEPYASPPQPGLP CCCCCCCCCCCCCCC	31.13	11038323
430	Phosphorylation	LVEVRALSMQAKEYL HHHHHHHHHHHHHHH	14.09	15523052

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
203	S	Phosphorylation	Kinase	CDK7	P50613	Uniprot
203	S	Phosphorylation	Kinase	MAPK1	P28482	GPS

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Reference
203	S	Phosphorylation	19015234
Phosphorylated on Ser-203 by CDK7.
203	S	Phosphorylation	19015234
Sumoylation reduces CDK7-mediated phosphorylation on Ser-203.
203	S	Sumoylation	19015234
Sumoylation reduces CDK7-mediated phosphorylation on Ser-203.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of STF1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
NRIP1_HUMAN	NRIP1	physical	12782406
NRIP1_HUMAN	NRIP1	physical	11459805
ZN653_HUMAN	ZNF653	physical	12920234
GATA4_HUMAN	GATA4	physical	10446911
NR0B1_HUMAN	NR0B1	physical	11713202
NCOA1_HUMAN	NCOA1	physical	9328345
TREF1_HUMAN	TRERF1	physical	12101186
CTNB1_HUMAN	CTNNB1	physical	12554773
SOX9_HUMAN	SOX9	physical	9774680
PITX1_HUMAN	PITX1	physical	10369682
CXXC1_HUMAN	CXXC1	physical	20211142
CBP_HUMAN	CREBBP	physical	10628748
BARD1_HUMAN	BARD1	physical	21087664
ARIP4_HUMAN	RAD54L2	physical	19692572
NR0B1_HUMAN	NR0B1	physical	14963109
NCK2_HUMAN	NCK2	physical	25416956
TPC2A_HUMAN	TRAPPC2	physical	20498720
TPC2B_HUMAN	TRAPPC2	physical	20498720

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
612965	46,XY sex reversal 3 (SRXY3)
184757	Adrenocortical insufficiency, without ovarian defect (ACIWOD)
612964	Premature ovarian failure 7 (POF7)
613957	Spermatogenic failure 8 (SPGF8)
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of STF1_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"SUMOylation inhibits SF-1 activity by reducing CDK7-mediated serine203 phosphorylation."; Yang W.-H., Heaton J.H., Brevig H., Mukherjee S., Iniguez-Lluhi J.A.,Hammer G.D.; Mol. Cell. Biol. 29:613-625(2009). Cited for: SUMOYLATION, AND PHOSPHORYLATION AT SER-203.	19015234 [Abstract]
"Phosphorylation of steroidogenic factor 1 is mediated by cyclin-dependent kinase 7."; Lewis A.E., Rusten M., Hoivik E.A., Vikse E.L., Hansson M.L.,Wallberg A.E., Bakke M.; Mol. Endocrinol. 22:91-104(2008). Cited for: PHOSPHORYLATION AT SER-203, AND INTERACTION WITH CDK7.	17901130 [Abstract]
"Phosphorylation of the nuclear receptor SF-1 modulates cofactorrecruitment: integration of hormone signaling in reproduction andstress."; Hammer G.D., Krylova I., Zhang Y., Darimont B.D., Simpson K.,Weigel N.L., Ingraham H.A.; Mol. Cell 3:521-526(1999). Cited for: PHOSPHORYLATION AT SER-203.	10230405 [Abstract]
Sumoylation
Reference	PubMed
"Small ubiquitin-like modifier 1 (SUMO-1) modification of the synergycontrol motif of Ad4 binding protein/steroidogenic factor 1 (Ad4BP/SF-1) regulates synergistic transcription between Ad4BP/SF-1 and Sox9."; Komatsu T., Mizusaki H., Mukai T., Ogawa H., Baba D., Shirakawa M.,Hatakeyama S., Nakayama K.I., Yamamoto H., Kikuchi A., Morohashi K.; Mol. Endocrinol. 18:2451-2462(2004). Cited for: SUMOYLATION AT LYS-119 AND LYS-194, AND MUTAGENESIS OF LYS-119 ANDLYS-194.	15192080 [Abstract]