SNP25_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: SNP25_HUMAN
• UniProt AC: P60880
• Protein Name: Synaptosomal-associated protein 25
• Gene Name: SNAP25
• Organism: Homo sapiens (Human).
• Sequence Length: 206
• Subcellular Localization: Cytoplasm, perinuclear region . Cell membrane ; Lipid-anchor . Cell junction, synapse, synaptosome . Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region. Colocalizes with KCNB1 at the
• Protein Description: t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells..
• Protein Sequence: MAEDADMRNELEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKDMKEAEKNLTDLGKFCGLCVCPCNKLKSSDAYKKAWGNNQDGVVASQPARVVDEREQMAISGGFIRRVTNDARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEANQRATKMLGSG

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
25	Phosphorylation	ADQLADESLESTRRM HHHHHHHHHHHHHHH	37.67	29214152
28	Phosphorylation	LADESLESTRRMLQL HHHHHHHHHHHHHHH	32.29	22311984
29	Phosphorylation	ADESLESTRRMLQLV HHHHHHHHHHHHHHH	16.96	22311984
40 (in isoform 2)	Ubiquitination	-	55.68	-
40	Ubiquitination	LQLVEESKDAGIRTL HHHHHHHHHCCCEEE	55.68	33845483
79	Ubiquitination	KEAEKNLTDLGKFCG HHHHHHHHHHHHHCC	38.27	33845483
85	S-palmitoylation	LTDLGKFCGLCVCPC HHHHHHHCCEEEECC	4.66	-
88	S-palmitoylation	LGKFCGLCVCPCNKL HHHHCCEEEECCCCC	1.47	-
90	S-palmitoylation	KFCGLCVCPCNKLKS HHCCEEEECCCCCCC	2.87	29575903
92	S-palmitoylation	CGLCVCPCNKLKSSD CCEEEECCCCCCCCH	6.24	29575903
96	Ubiquitination	VCPCNKLKSSDAYKK EECCCCCCCCHHHHH	49.91	33845483
102	Ubiquitination	LKSSDAYKKAWGNNQ CCCCHHHHHHHCCCC	37.42	32142685
103	Ubiquitination	KSSDAYKKAWGNNQD CCCHHHHHHHCCCCC	36.37	30230243
115	Phosphorylation	NQDGVVASQPARVVD CCCCCCCCCCCEECC	25.70	24076635
130	Phosphorylation	EREQMAISGGFIRRV HHHHHHHHCCHHHHH	24.27	27732954
138	Phosphorylation	GGFIRRVTNDARENE CCHHHHHCCCHHHHH	26.10	22311984
154	Phosphorylation	DENLEQVSGIIGNLR CCCHHHHHHHHHHHH	24.78	-
184	Ubiquitination	QIDRIMEKADSNKTR HHHHHHHHHHHCCCH	39.86	30230243
187	Phosphorylation	RIMEKADSNKTRIDE HHHHHHHHCCCHHHH	45.12	22311984
201	Ubiquitination	EANQRATKMLGSG-- HHHHHHHHHHCCC--	31.01	-
201 (in isoform 2)	Ubiquitination	-	31.01	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
28	S	Phosphorylation	Kinase	PKACA	P17612	PSP
29	T	Phosphorylation	Kinase	PKACA	P17612	PSP
138	T	Phosphorylation	Kinase	PKACA	P17612	PSP
138	T	Phosphorylation	Kinase	PRKCA	P17252	GPS
187	S	Phosphorylation	Kinase	PKACA	P17612	PSP
187	S	Phosphorylation	Kinase	PRKCA	P17252	GPS

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
85	C	Palmitoylation	Cys-85 appears to be the main site, and palmitoylation is required for membrane association (By similarity).	28757145

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of SNP25_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
STX11_HUMAN	STX11	physical	16189514
PRRT2_HUMAN	PRRT2	physical	16169070
STX11_HUMAN	STX11	physical	16169070
STX1A_HUMAN	STX1A	physical	16169070
KINH_HUMAN	KIF5B	physical	12475239
GOSR1_HUMAN	GOSR1	physical	9325254
STX5_HUMAN	STX5	physical	9325254
ITSN1_HUMAN	ITSN1	physical	10373452
STX12_HUMAN	STX12	physical	10886332
SNAPN_HUMAN	SNAPIN	physical	10195194
STX1A_HUMAN	STX1A	physical	10195194
TRIM9_HUMAN	TRIM9	physical	11524423
VAMP2_HUMAN	VAMP2	physical	11524423
STX1A_HUMAN	STX1A	physical	11524423
STX1A_HUMAN	STX1A	physical	7961655
VAMP2_HUMAN	VAMP2	physical	9030619
VAMP2_HUMAN	VAMP2	physical	11832227
SYT1_HUMAN	SYT1	physical	22890573
VAMP2_HUMAN	VAMP2	physical	22890573
STX1A_HUMAN	STX1A	physical	20489724
A4_HUMAN	APP	physical	21832049
WASC3_HUMAN	CCDC53	physical	19060904
STXB1_HUMAN	STXBP1	physical	26344197

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• 616330: Myasthenic syndrome, congenital, 18 (CMS18)
• Kegg Drug
• D10012: Incobotulinumtoxin A (USAN); Onaclostox; Xeomin (TN)
• DrugBank
• There are no disease associations of PTM sites.