PIWI_DROME - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: PIWI_DROME
• UniProt AC: Q9VKM1
• Protein Name: Protein piwi
• Gene Name: piwi
• Organism: Drosophila melanogaster (Fruit fly).
• Sequence Length: 843
• Subcellular Localization: Cytoplasm. Nucleus, nucleoplasm. Component of polar granules. Present in the cytoplasm of the developing oocyte. At the mitotic cycle 11 translocates to the nucleus where it remains localized throughout germ-cell development and gonadogenesis. Locali
• Protein Description: Acts via the piwi-interacting RNA (piRNA) metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. In ovarian somatic cells, mediates silencing of transposable elements at the transcriptional level in a mael-dependent manner. In germ cells, mediates silencing at both transcriptional and post-transcriptional levels and is involved in the maintenance of populations of primary and secondary piRNAs. Piwi-mediated transcriptional silencing is accompanied by the formation of His3 'Lys-9' trimethylated (H3K9me3) euchromatin and heterochromatin. In ovary, associates predominantly with antisense piRNAs that contain uridine at their 5' end. Association with sense piRNAs is also observed but to a lesser extent. Involved in silencing of long terminal repeat (LTR) retrotransposons in male germline. Mediates a somatic signaling mechanism required for the maintenance of germline stem cells to produce and maintain a daughter germline stem cell. It is not essential for the further differentiation of the committed daughter cell. Acts cell autonomously to promote germline stem cell division. Its role in stem cell maintenance does not seem to require nuclear localization. Required maternally for the posterior localization of osk and vas and for pole cell formation during oogenesis and early embryogenesis. Together with Hop and Hsp83, mediates canalization, also known as developmental robustness, likely via epigenetic silencing of existing genetic variants and suppression of transposon-induced new genetic variation. Shows RNA cleavage activity, although is not required for any of its known functions..
• Protein Sequence: MADDQGRGRRRPLNEDDSSTSRGSGDGPRVKVFRGSSSGDPRADPRIEASRERRALEEAPRREGGPTERKPWGDQYDYLNTRPAELVSKKGTDGVPVMLQTNFFRLKTKPEWRIVHYHVEFEPSIENPRVRMGVLSNHANLLGSGYLFDGLQLFTTRKFEQEITVLSGKSKLDIEYKISIKFVGFISCAEPRFLQVLNLILRRSMKGLNLELVGRNLFDPRAKIEIREFKMELWPGYETSIRQHEKDILLGTEITHKVMRTETIYDIMRRCSHNPARHQDEVRVNVLDLIVLTDYNNRTYRINDVDFGQTPKSTFSCKGRDISFVEYYLTKYNIRIRDHNQPLLISKNRDKALKTNASELVVLIPELCRVTGLNAEMRSNFQLMRAMSSYTRMNPKQRTDRLRAFNHRLQNTPESVKVLRDWNMELDKNVTEVQGRIIGQQNIVFHNGKVPAGENADWQRHFRDQRMLTTPSDGLDRWAVIAPQRNSHELRTLLDSLYRAASGMGLRIRSPQEFIIYDDRTGTYVRAMDDCVRSDPKLILCLVPNDNAERYSSIKKRGYVDRAVPTQVVTLKTTKNRSLMSIATKIAIQLNCKLGYTPWMIELPLSGLMTIGFDIAKSTRDRKRAYGALIASMDLQQNSTYFSTVTECSAFDVLANTLWPMIAKALRQYQHEHRKLPSRIVFYRDGVSSGSLKQLFEFEVKDIIEKLKTEYARVQLSPPQLAYIVVTRSMNTRFFLNGQNPPPGTIVDDVITLPERYDFYLVSQQVRQGTVSPTSYNVLYSSMGLSPEKMQKLTYKMCHLYYNWSGTTRVPAVCQYAKKLATLVGTNLHSIPQNALEKKFYYL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
7	Methylation	-MADDQGRGRRRPLN -CCCCCCCCCCCCCC	29.79	29531043
9	Methylation	ADDQGRGRRRPLNED CCCCCCCCCCCCCCC	30.03	29531043
10	Methylation	DDQGRGRRRPLNEDD CCCCCCCCCCCCCCC	47.60	29531043
11	Methylation	DQGRGRRRPLNEDDS CCCCCCCCCCCCCCC	38.40	29531043
67	Phosphorylation	PRREGGPTERKPWGD CCCCCCCCCCCCCCC	52.26	19429919
510	Phosphorylation	GMGLRIRSPQEFIIY CCCCEECCCCEEEEE	28.67	18511481
517	Phosphorylation	SPQEFIIYDDRTGTY CCCEEEEEECCCCCE	13.63	18511481

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of PIWI_DROME !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of PIWI_DROME !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of PIWI_DROME !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
ARNT_DROME	tgo	genetic	14704180
HH_DROME	hh	genetic	11463375
RS3_DROME	RpS3	genetic	14704180
S39AD_DROME	Zip99C	genetic	14704180
SRYD_DROME	Sry-delta	genetic	14704180
SIMA_DROME	sima	genetic	14704180
HP1_DROME	Su(var)205	physical	17875665
ESC_DROME	esc	physical	26780607
MUT7_DROME	Nbr	physical	26718004
FMR1_DROME	Fmr1	physical	16949822
FMR1_DROME	Fmr1	physical	26842990
FOSLD_DROME	kay	physical	27622269
FOSLA_DROME	kay	physical	27622269
ARMI_DROME	armi	physical	20818334
ARMI_DROME	armi	physical	20966047
EZ_DROME	E(z)	physical	26780607
CORTO_DROME	corto	physical	26780607
DCR1_DROME	Dcr-1	physical	16949822
SUZ12_DROME	Su(z)12	physical	26780607

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.