dbPTM

NFX1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	NFX1_HUMAN
UniProt AC	Q12986
Protein Name	Transcriptional repressor NF-X1
Gene Name	NFX1
Organism	Homo sapiens (Human).
Sequence Length	1120
Subcellular Localization	Nucleus.
Protein Description	Binds to the X-box motif of MHC class II genes and represses their expression. May play an important role in regulating the duration of an inflammatory response by limiting the period in which MHC class II molecules are induced by interferon-gamma. Isoform 3 binds to the X-box motif of TERT promoter and represses its expression. Together with PABPC1 or PABPC4, isoform 1 acts as a coactivator for TERT expression. Mediates E2-dependent ubiquitination..
Protein Sequence	MAEAPPVSGTFKFNTDAAEFIPQEKKNSGLNCGTQRRLDSNRIGRRNYSSPPPCHLSRQVPYDEISAVHQHSYHPSGSKPKSQQTSFQSSPCNKSPKSHGLQNQPWQKLRNEKHHIRVKKAQSLAEQTSDTAGLESSTRSESGTDLREHSPSESEKEVVGADPRGAKPKKATQFVYSYGRGPKVKGKLKCEWSNRTTPKPEDAGPESTKPVGVFHPDSSEASSRKGVLDGYGARRNEQRRYPQKRPPWEVEGARPRPGRNPPKQEGHRHTNAGHRNNMGPIPKDDLNERPAKSTCDSENLAVINKSSRRVDQEKCTVRRQDPQVVSPFSRGKQNHVLKNVETHTGSLIEQLTTEKYECMVCCELVRVTAPVWSCQSCYHVFHLNCIKKWARSPASQADGQSGWRCPACQNVSAHVPNTYTCFCGKVKNPEWSRNEIPHSCGEVCRKKQPGQDCPHSCNLLCHPGPCPPCPAFMTKTCECGRTRHTVRCGQAVSVHCSNPCENILNCGQHQCAELCHGGQCQPCQIILNQVCYCGSTSRDVLCGTDVGKSDGFGDFSCLKICGKDLKCGNHTCSQVCHPQPCQQCPRLPQLVRCCPCGQTPLSQLLELGSSSRKTCMDPVPSCGKVCGKPLPCGSLDFIHTCEKLCHEGDCGPCSRTSVISCRCSFRTKELPCTSLKSEDATFMCDKRCNKKRLCGRHKCNEICCVDKEHKCPLICGRKLRCGLHRCEEPCHRGNCQTCWQASFDELTCHCGASVIYPPVPCGTRPPECTQTCARVHECDHPVYHSCHSEEKCPPCTFLTQKWCMGKHEFRSNIPCHLVDISCGLPCSATLPCGMHKCQRLCHKGECLVDEPCKQPCTTPRADCGHPCMAPCHTSSPCPVTACKAKVELQCECGRRKEMVICSEASSTYQRIAAISMASKITDMQLGGSVEISKLITKKEVHQARLECDEECSALERKKRLAEAFHISEDSDPFNIRSSGSKFSDSLKEDARKDLKFVSDVEKEMETLVEAVNKGKNSKKSHSFPPMNRDHRRIIHDLAQVYGLESVSYDSEPKRNVVVTAIRGKSVCPPTTLTGVLEREMQARPPPPIPHHRHQSDKNPGSSNLQKITKEPIIDYFDVQD

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
8	Phosphorylation	MAEAPPVSGTFKFNT CCCCCCCCCEEEECC	37.58	24719451
25	Ubiquitination	AEFIPQEKKNSGLNC HHHCCHHHCCCCCCC	52.59	29967540
34	Phosphorylation	NSGLNCGTQRRLDSN CCCCCCCCCCCCCCC	22.54	-
48	Phosphorylation	NRIGRRNYSSPPPCH CCCCCCCCCCCCCCC	14.55	22167270
49	Phosphorylation	RIGRRNYSSPPPCHL CCCCCCCCCCCCCCC	39.66	22167270
50	Phosphorylation	IGRRNYSSPPPCHLS CCCCCCCCCCCCCCC	30.94	22167270
57	Phosphorylation	SPPPCHLSRQVPYDE CCCCCCCCCCCCHHH	9.30	23927012
62	Phosphorylation	HLSRQVPYDEISAVH CCCCCCCHHHHHHHE	27.61	26074081
82	Phosphorylation	PSGSKPKSQQTSFQS CCCCCCCCCCCCCCC	35.73	23927012
85	Phosphorylation	SKPKSQQTSFQSSPC CCCCCCCCCCCCCCC	24.71	28450419
86	Phosphorylation	KPKSQQTSFQSSPCN CCCCCCCCCCCCCCC	19.56	28450419
89	Phosphorylation	SQQTSFQSSPCNKSP CCCCCCCCCCCCCCC	33.95	25159151
90	Phosphorylation	QQTSFQSSPCNKSPK CCCCCCCCCCCCCCC	23.76	25159151
95	Phosphorylation	QSSPCNKSPKSHGLQ CCCCCCCCCCCCCCC	24.46	23927012
97	Ubiquitination	SPCNKSPKSHGLQNQ CCCCCCCCCCCCCCC	63.27	29967540
98	Phosphorylation	PCNKSPKSHGLQNQP CCCCCCCCCCCCCCH	26.60	27174698
108	Ubiquitination	LQNQPWQKLRNEKHH CCCCHHHHHHCCHHH	45.61	32015554
120	Ubiquitination	KHHIRVKKAQSLAEQ HHHHHHHHHHHHHHH	49.12	29967540
123	Phosphorylation	IRVKKAQSLAEQTSD HHHHHHHHHHHHCCC	34.11	25159151
128	Phosphorylation	AQSLAEQTSDTAGLE HHHHHHHCCCCCCCC	21.78	27732954
129	Phosphorylation	QSLAEQTSDTAGLES HHHHHHCCCCCCCCC	32.43	27732954
131	Phosphorylation	LAEQTSDTAGLESST HHHHCCCCCCCCCCC	23.53	27732954
136	Phosphorylation	SDTAGLESSTRSESG CCCCCCCCCCCCCCC	41.89	25159151
137	Phosphorylation	DTAGLESSTRSESGT CCCCCCCCCCCCCCC	20.61	25159151
138	Phosphorylation	TAGLESSTRSESGTD CCCCCCCCCCCCCCC	47.71	25159151
140	Phosphorylation	GLESSTRSESGTDLR CCCCCCCCCCCCCHH	35.88	23403867
142	Phosphorylation	ESSTRSESGTDLREH CCCCCCCCCCCHHHC	48.52	23401153
144	Phosphorylation	STRSESGTDLREHSP CCCCCCCCCHHHCCC	40.93	25159151
150	Phosphorylation	GTDLREHSPSESEKE CCCHHHCCCCHHHHH	25.93	29255136
152	Phosphorylation	DLREHSPSESEKEVV CHHHCCCCHHHHHCC	57.69	23927012
154	Phosphorylation	REHSPSESEKEVVGA HHCCCCHHHHHCCCC	59.30	25159151
172	Phosphorylation	GAKPKKATQFVYSYG CCCCCCCCCCEEECC	31.27	21945579
176	Phosphorylation	KKATQFVYSYGRGPK CCCCCCEEECCCCCC	9.47	21945579
177	Phosphorylation	KATQFVYSYGRGPKV CCCCCEEECCCCCCC	18.79	21945579
178	Phosphorylation	ATQFVYSYGRGPKVK CCCCEEECCCCCCCC	7.99	21945579
193	Phosphorylation	GKLKCEWSNRTTPKP CEEEEEECCCCCCCC	8.30	27251275
197	Phosphorylation	CEWSNRTTPKPEDAG EEECCCCCCCCCCCC	26.52	28555341
199	Ubiquitination	WSNRTTPKPEDAGPE ECCCCCCCCCCCCCC	59.97	29967540
209	Ubiquitination	DAGPESTKPVGVFHP CCCCCCCCCCEEECC	47.16	29967540
218	Phosphorylation	VGVFHPDSSEASSRK CEEECCCCCCHHHCC	34.23	27732954
219	Phosphorylation	GVFHPDSSEASSRKG EEECCCCCCHHHCCC	44.56	27732954
222	Phosphorylation	HPDSSEASSRKGVLD CCCCCCHHHCCCCCC	26.99	27732954
223	Phosphorylation	PDSSEASSRKGVLDG CCCCCHHHCCCCCCC	45.09	27732954
225	Ubiquitination	SSEASSRKGVLDGYG CCCHHHCCCCCCCCC	56.15	33845483
231	Phosphorylation	RKGVLDGYGARRNEQ CCCCCCCCCCCCCCC	13.79	-
283	Ubiquitination	NNMGPIPKDDLNERP CCCCCCCHHHCCCCC	65.67	29967540
292	Methylation	DLNERPAKSTCDSEN HCCCCCCCCCCCHHC	49.32	23644510
294	Phosphorylation	NERPAKSTCDSENLA CCCCCCCCCCHHCEE	21.45	28555341
305	Ubiquitination	ENLAVINKSSRRVDQ HCEEEEECCCCCCCH	38.39	32015554
314	Ubiquitination	SRRVDQEKCTVRRQD CCCCCHHHCCCCCCC	29.58	24816145
316	Phosphorylation	RVDQEKCTVRRQDPQ CCCHHHCCCCCCCCC	28.28	-
326	Phosphorylation	RQDPQVVSPFSRGKQ CCCCCCCCCCCCCCC	22.75	19664994
329	Phosphorylation	PQVVSPFSRGKQNHV CCCCCCCCCCCCCCE	43.43	30266825
342	Phosphorylation	HVLKNVETHTGSLIE CEECCCCHHHCCHHH	22.06	27251275
344	Phosphorylation	LKNVETHTGSLIEQL ECCCCHHHCCHHHHH	35.57	27251275
346	Phosphorylation	NVETHTGSLIEQLTT CCCHHHCCHHHHHHC	27.59	20873877
392	Phosphorylation	CIKKWARSPASQADG HHHHHHCCHHHHCCC	19.64	25159151
427	Ubiquitination	TCFCGKVKNPEWSRN ECCCCCCCCCCCCCC	70.98	32015554
548	Ubiquitination	LCGTDVGKSDGFGDF EECCCCCCCCCCCCC	45.26	32015554
559	Ubiquitination	FGDFSCLKICGKDLK CCCCHHEEEECCCCC	40.03	32015554
599	Phosphorylation	RCCPCGQTPLSQLLE CCCCCCCCCHHHHHH	16.10	-
602	Phosphorylation	PCGQTPLSQLLELGS CCCCCCHHHHHHHCC	21.71	-
609	Phosphorylation	SQLLELGSSSRKTCM HHHHHHCCCCCCCCC	37.45	-
613	Ubiquitination	ELGSSSRKTCMDPVP HHCCCCCCCCCCCCC	48.28	29967540
628	Ubiquitination	SCGKVCGKPLPCGSL CCCCCCCCCCCCCCC	37.95	32015554
634	Phosphorylation	GKPLPCGSLDFIHTC CCCCCCCCCHHHHHH	31.33	27251275
664	Phosphorylation	SVISCRCSFRTKELP EEEEEEEEEECCCCC	9.83	-
668	Ubiquitination	CRCSFRTKELPCTSL EEEEEECCCCCCCCC	53.66	29967540
676	Ubiquitination	ELPCTSLKSEDATFM CCCCCCCCCCCCCEE	52.12	32015554
677	Phosphorylation	LPCTSLKSEDATFMC CCCCCCCCCCCCEEC	46.10	-
681	Phosphorylation	SLKSEDATFMCDKRC CCCCCCCCEECCCCC	24.99	-
686	Acetylation	DATFMCDKRCNKKRL CCCEECCCCCCCCCC	54.65	27452117
686	Ubiquitination	DATFMCDKRCNKKRL CCCEECCCCCCCCCC	54.65	32015554
690	Ubiquitination	MCDKRCNKKRLCGRH ECCCCCCCCCCCCCC	42.78	-
691	Ubiquitination	CDKRCNKKRLCGRHK CCCCCCCCCCCCCCC	36.29	-
710	Ubiquitination	CCVDKEHKCPLICGR EECCCCCCCCEECCC	38.34	29967540
711	Ubiquitination	CVDKEHKCPLICGRK ECCCCCCCCEECCCE	3.41	29967540
783	Phosphorylation	HECDHPVYHSCHSEE HCCCCCCCCCCCCCC	7.77	-
785	Phosphorylation	CDHPVYHSCHSEEKC CCCCCCCCCCCCCCC	8.88	28555341
788	Phosphorylation	PVYHSCHSEEKCPPC CCCCCCCCCCCCCCC	52.34	29449344
811	Phosphorylation	MGKHEFRSNIPCHLV CCCCHHHHCCCEEEE	44.42	22210691
812 (in isoform 3)	Phosphorylation	-	37.66	-
818	Ubiquitination	SNIPCHLVDISCGLP HCCCEEEEEECCCCC	2.46	29967540
853	Ubiquitination	CLVDEPCKQPCTTPR CCCCCCCCCCCCCCC	68.91	32015554
854	Ubiquitination	LVDEPCKQPCTTPRA CCCCCCCCCCCCCCC	45.04	32015554
857	Phosphorylation	EPCKQPCTTPRADCG CCCCCCCCCCCCCCC	46.66	27251275
858	Phosphorylation	PCKQPCTTPRADCGH CCCCCCCCCCCCCCC	19.93	27251275
885	Ubiquitination	PVTACKAKVELQCEC CCCCCEEEEEEEEEC	23.03	21906983
885 (in isoform 1)	Ubiquitination	-	23.03	21906983
885 (in isoform 2)	Ubiquitination	-	23.03	21906983
907	Phosphorylation	ICSEASSTYQRIAAI EEECCCCHHHHHHHH	22.60	-
928	Phosphorylation	TDMQLGGSVEISKLI CCCCCCCCEEEHHCC	18.12	28555341
967	Phosphorylation	LAEAFHISEDSDPFN HHHHHCCCCCCCCCC	27.12	24719451
977	Phosphorylation	SDPFNIRSSGSKFSD CCCCCCCCCCCCCCH	34.94	25627689
978	Phosphorylation	DPFNIRSSGSKFSDS CCCCCCCCCCCCCHH	36.92	25159151
980	Phosphorylation	FNIRSSGSKFSDSLK CCCCCCCCCCCHHHH	32.26	24719451
981	Acetylation	NIRSSGSKFSDSLKE CCCCCCCCCCHHHHH	53.03	7705889
981	Ubiquitination	NIRSSGSKFSDSLKE CCCCCCCCCCHHHHH	53.03	29967540
982	Ubiquitination	IRSSGSKFSDSLKED CCCCCCCCCHHHHHH	11.98	29967540
983	Phosphorylation	RSSGSKFSDSLKEDA CCCCCCCCHHHHHHH	29.99	27251275
985	Phosphorylation	SGSKFSDSLKEDARK CCCCCCHHHHHHHHH	39.89	27251275
995	Ubiquitination	EDARKDLKFVSDVEK HHHHHHHCHHHHHHH	54.58	29967540
996	Ubiquitination	DARKDLKFVSDVEKE HHHHHHCHHHHHHHH	9.16	29967540
1013	Ubiquitination	TLVEAVNKGKNSKKS HHHHHHHCCCCCCCC	65.74	29967540
1014	Ubiquitination	LVEAVNKGKNSKKSH HHHHHHCCCCCCCCC	29.22	29967540
1041	Phosphorylation	IHDLAQVYGLESVSY HHHHHHHHCCCCCCC	12.15	18187866
1045	Phosphorylation	AQVYGLESVSYDSEP HHHHCCCCCCCCCCC	22.77	18187866
1047	Phosphorylation	VYGLESVSYDSEPKR HHCCCCCCCCCCCCC	32.28	18187866
1095	Phosphorylation	IPHHRHQSDKNPGSS CCCCCCCCCCCCCCC	44.41	23401153
1097	Ubiquitination	HHRHQSDKNPGSSNL CCCCCCCCCCCCCCH	71.00	29967540
1098	Ubiquitination	HRHQSDKNPGSSNLQ CCCCCCCCCCCCCHH	51.67	29967540
1106	Ubiquitination	PGSSNLQKITKEPII CCCCCHHHHHCCCEE	56.35	29967540
1107	Ubiquitination	GSSNLQKITKEPIID CCCCHHHHHCCCEEC	4.25	29967540
1115	Phosphorylation	TKEPIIDYFDVQD-- HCCCEECCCCCCC--	7.41	28796482

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of NFX1_HUMAN !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of NFX1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of NFX1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
MAML3_HUMAN	MAML3	physical	20211142
SIN3A_HUMAN	SIN3A	physical	18505829
USP9X_HUMAN	USP9X	physical	17267499
PABP1_HUMAN	PABPC1	physical	17267499
EF1A2_HUMAN	EEF1A2	physical	17267499
MATR3_HUMAN	MATR3	physical	17267499
PABP3_HUMAN	PABPC3	physical	17267499
HNRPM_HUMAN	HNRNPM	physical	17267499
HNRPU_HUMAN	HNRNPU	physical	17267499
RL13_HUMAN	RPL13	physical	17267499
SRSF4_HUMAN	SRSF4	physical	17267499
H2A1B_HUMAN	HIST1H2AE	physical	17267499
RL7A_HUMAN	RPL7A	physical	17267499
F208B_HUMAN	FAM208B	physical	17267499
LARP1_HUMAN	LARP1	physical	17267499
DDX5_HUMAN	DDX5	physical	17267499
DMD_HUMAN	DMD	physical	17267499
NUCL_HUMAN	NCL	physical	17267499
RL13A_HUMAN	RPL13A	physical	17267499
RL17_HUMAN	RPL17	physical	17267499
RL22_HUMAN	RPL22	physical	17267499
RL3_HUMAN	RPL3	physical	17267499
RL6_HUMAN	RPL6	physical	17267499
ROA2_HUMAN	HNRNPA2B1	physical	17267499
HNRH1_HUMAN	HNRNPH1	physical	17267499
RS19_HUMAN	RPS19	physical	17267499
RS3A_HUMAN	RPS3A	physical	17267499
SRSF3_HUMAN	SRSF3	physical	17267499
APCL_HUMAN	APC2	physical	17267499
YBOX1_HUMAN	YBX1	physical	17267499

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of NFX1_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Large-scale proteomics analysis of the human kinome."; Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G.,Mann M., Daub H.; Mol. Cell. Proteomics 8:1751-1764(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-50, AND MASSSPECTROMETRY.	19369195 [Abstract]
"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach."; Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.; Anal. Chem. 81:4493-4501(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-90, AND MASSSPECTROMETRY.	19413330 [Abstract]
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-50, AND MASSSPECTROMETRY.	18669648 [Abstract]
"Combining protein-based IMAC, peptide-based IMAC, and MudPIT forefficient phosphoproteomic analysis."; Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D.,Yates J.R. III; J. Proteome Res. 7:1346-1351(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-49 AND SER-50, AND MASSSPECTROMETRY.	18220336 [Abstract]
"Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column."; Imami K., Sugiyama N., Kyono Y., Tomita M., Ishihama Y.; Anal. Sci. 24:161-166(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-1041, AND MASSSPECTROMETRY.	18187866 [Abstract]