MYLIP_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: MYLIP_HUMAN
• UniProt AC: Q8WY64
• Protein Name: E3 ubiquitin-protein ligase MYLIP
• Gene Name: MYLIP
• Organism: Homo sapiens (Human).
• Sequence Length: 445
• Subcellular Localization: Cytoplasm . Cell membrane ; Peripheral membrane protein .
• Protein Description: E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Activity depends on E2 enzymes of the UBE2D family. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol-dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR..
• Protein Sequence: MLCYVTRPDAVLMEVEVEAKANGEDCLNQVCRRLGIIEVDYFGLQFTGSKGESLWLNLRNRISQQMDGLAPYRLKLRVKFFVEPHLILQEQTRHIFFLHIKEALLAGHLLCSPEQAVELSALLAQTKFGDYNQNTAKYNYEELCAKELSSATLNSIVAKHKELEGTSQASAEYQVLQIVSAMENYGIEWHSVRDSEGQKLLIGVGPEGISICKDDFSPINRIAYPVVQMATQSGKNVYLTVTKESGNSIVLLFKMISTRAASGLYRAITETHAFYRCDTVTSAVMMQYSRDLKGHLASLFLNENINLGKKYVFDIKRTSKEVYDHARRALYNAGVVDLVSRNNQSPSHSPLKSSESSMNCSSCEGLSCQQTRVLQEKLRKLKEAMLCMVCCEEEINSTFCPCGHTVCCESCAAQLQSCPVCRSRVEHVQHVYLPTHTSLLNLTVI

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
4	Phosphorylation	----MLCYVTRPDAV ----CCEEEECCCEE	10.82	23663014
6	Phosphorylation	--MLCYVTRPDAVLM --CCEEEECCCEEEE	15.26	23663014
47	Phosphorylation	DYFGLQFTGSKGESL EEEEEEEECCCCCEE	27.82	-
49	Phosphorylation	FGLQFTGSKGESLWL EEEEEECCCCCEEHH	34.14	-
137	Ubiquitination	DYNQNTAKYNYEELC CCCCCHHCCCHHHHH	31.80	-
146	Ubiquitination	NYEELCAKELSSATL CHHHHHHHHHHHHHH	59.18	-
149	Phosphorylation	ELCAKELSSATLNSI HHHHHHHHHHHHHHH	21.01	25159151
150	Phosphorylation	LCAKELSSATLNSIV HHHHHHHHHHHHHHH	37.59	26074081
152	Phosphorylation	AKELSSATLNSIVAK HHHHHHHHHHHHHHH	28.55	26074081
159	Ubiquitination	TLNSIVAKHKELEGT HHHHHHHHHHHHCCC	44.29	-
257	Phosphorylation	VLLFKMISTRAASGL EEEEEHHHHHHHHHH	14.35	22210691
258	Phosphorylation	LLFKMISTRAASGLY EEEEHHHHHHHHHHH	17.25	22210691
269	Phosphorylation	SGLYRAITETHAFYR HHHHHHHHHCCCCCC	33.57	30576142
279	Phosphorylation	HAFYRCDTVTSAVMM CCCCCCCHHHHHHHH	29.34	29759185
281	Phosphorylation	FYRCDTVTSAVMMQY CCCCCHHHHHHHHHH	17.04	29759185
282	Phosphorylation	YRCDTVTSAVMMQYS CCCCHHHHHHHHHHC	17.96	30576142
318	Phosphorylation	YVFDIKRTSKEVYDH EEEEEECCCHHHHHH	38.87	-
319	Phosphorylation	VFDIKRTSKEVYDHA EEEEECCCHHHHHHH	30.74	-
323	Phosphorylation	KRTSKEVYDHARRAL ECCCHHHHHHHHHHH	11.78	-
377	Ubiquitination	QTRVLQEKLRKLKEA HHHHHHHHHHHHHHH	40.40	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
-	K	Ubiquitination	E3 ubiquitin ligase	MYLIP	Q8WY64	PMID:14550572

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of MYLIP_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of MYLIP_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
CNPY2_HUMAN	CNPY2	physical	12826659
MYLIP_HUMAN	MYLIP	physical	12826659
LDLR_HUMAN	LDLR	physical	21734303
MYLIP_HUMAN	MYLIP	physical	21685362
LDLR_HUMAN	LDLR	physical	21685362
UB2D1_HUMAN	UBE2D1	physical	21685362
LDLR_HUMAN	LDLR	physical	22109552
VLDLR_HUMAN	VLDLR	physical	22109552
LRP8_HUMAN	LRP8	physical	22109552
ML12B_HUMAN	MYL12B	physical	10593918
UB2D2_HUMAN	UBE2D2	physical	21685362
UB2D3_HUMAN	UBE2D3	physical	21685362
UB2D4_HUMAN	UBE2D4	physical	21685362
UB2D1_HUMAN	UBE2D1	physical	21734303
UBE2N_HUMAN	UBE2N	physical	21734303
KDM1A_HUMAN	KDM1A	physical	23455924
T22D4_HUMAN	TSC22D4	physical	25416956
RASF5_HUMAN	RASSF5	physical	25416956
MIPO1_HUMAN	MIPOL1	physical	25416956
LDLR_HUMAN	LDLR	physical	23382078
CTR9_HUMAN	CTR9	physical	28514442
WDFY1_HUMAN	WDFY1	physical	28514442
MYCB2_HUMAN	MYCBP2	physical	28514442
PAF1_HUMAN	PAF1	physical	28514442
UBA6_HUMAN	UBA6	physical	28514442

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.