MPK3_ARATH - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: MPK3_ARATH
• UniProt AC: Q39023
• Protein Name: Mitogen-activated protein kinase 3
• Gene Name: MPK3
• Organism: Arabidopsis thaliana (Mouse-ear cress).
• Sequence Length: 370
• Subcellular Localization: Cytoplasm . Nucleus . Translocated into the nucleus in response to phosphorylation.
• Protein Description: Involved in oxidative stress-mediated signaling cascade (such as ozone). Involved in the innate immune MAP kinase signaling cascade (MEKK1, MKK4/MKK5 and MPK3/MPK6) downstream of bacterial flagellin receptor FLS2. May be involved in hypersensitive response (HR)-mediated signaling cascade by modulating LIP5 phosphorylation and subsequent multivesicular bodies (MVBs) trafficking. May phosphorylate regulators of WRKY transcription factors. Mediates the phosphorylation of VIP1 and subsequent stress genes transcription in response to Agrobacterium. MKK9-MPK3/MPK6 module phosphorylates and activates EIN3, leading to the promotion of EIN3-mediated transcription in ethylene signaling. MPK3/MPK6 cascade regulates camalexin synthesis through transcriptional regulation of the biosynthetic genes after pathogen infection. YDA-MKK4/MKK5-MPK3/MPK6 module regulates stomatal cell fate before the guard mother cell (GMC) is specified. This MAPK cascade also functions downstream of the ER receptor in regulating coordinated local cell proliferation, which shapes the morphology of plant organs..
• Protein Sequence: MNTGGGQYTDFPAVETHGGQFISYDIFGSLFEITSKYRPPIIPIGRGAYGIVCSVLDTETNELVAMKKIANAFDNHMDAKRTLREIKLLRHLDHENIIAIRDVVPPPLRRQFSDVYISTELMDTDLHQIIRSNQSLSEEHCQYFLYQLLRGLKYIHSANIIHRDLKPSNLLLNANCDLKICDFGLARPTSENDFMTEYVVTRWYRAPELLLNSSDYTAAIDVWSVGCIFMELMNRKPLFPGKDHVHQMRLLTELLGTPTESDLGFTHNEDAKRYIRQLPNFPRQPLAKLFSHVNPMAIDLVDRMLTFDPNRRITVEQALNHQYLAKLHDPNDEPICQKPFSFEFEQQPLDEEQIKEMIYQEAIALNPTYG

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
196	Phosphorylation	TSENDFMTEYVVTRW CCCCCHHHHHHHHHE	24.94	29654922
198	Phosphorylation	ENDFMTEYVVTRWYR CCCHHHHHHHHHEEC	7.34	22074104
201	Phosphorylation	FMTEYVVTRWYRAPE HHHHHHHHHEECCHH	12.87	22074104
314	Phosphorylation	FDPNRRITVEQALNH CCCCCCEEHHHHHCH	19.04	19880383

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of MPK3_ARATH !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
196	T	Phosphorylation	Dually phosphorylated on Thr-196 and Tyr-198, which activates the enzyme (By similarity).	11874576

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of MPK3_ARATH !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
NIA1_ARATH	NIA1	physical	20870959
NIA2_ARATH	NIA2	physical	20870959
Y2706_ARATH	AT2G47060	physical	21276203
OXI1_ARATH	AGC2-1	physical	21276203
ZAT10_ARATH	STZ	physical	22134874
AK1_ARATH	AK-LYS1	physical	21798944
EF103_ARATH	ERF6	physical	23300166
MYB44_ARATH	MYBR1	physical	23437396
ERLL1_ARATH	AZI1	physical	24214892
DRB1_ARATH	HYL1	physical	25417716
HFA4A_ARATH	HSF A4A	physical	24676858
CDKC1_ARATH	CDKC;1	physical	25464831
CDKC2_ARATH	CDKC2	physical	25464831
VIP1_ARATH	VIP1	physical	17947581
NRPB1_ARATH	NRPB1	physical	19467629
MPK3_ARATH	MPK3	physical	25969537

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.