MED25_ARATH - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: MED25_ARATH
• UniProt AC: Q7XYY2
• Protein Name: Mediator of RNA polymerase II transcription subunit 25
• Gene Name: MED25
• Organism: Arabidopsis thaliana (Mouse-ear cress).
• Sequence Length: 836
• Subcellular Localization: Nucleus .
• Protein Description: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Positive regulator of shade avoidance and of jasmonate signaling. Acts in repression of PhyB-mediated light signaling and regulates the expression of FLOWERING LOCUS T (FT) and of CONSTANS (CO)..
• Protein Sequence: MSSEVKQLIVVAEGTAALGPYWQTIVSDYLEKIIRSFCGSELNGERNPVSTVELSLVIFNSHGSYCACLVQRSGWTRDVDIFLHWLSSIQFGGGGFNEVATAEGLAEALMMFSPPSGQAQPSNDLKRHCILITASNPHILPTPVYRPRLQNVERNENGDAQAESRLSDAETVASYFAKCSVSLSVVCPKQLPTIRALYNAGKPNQQSADLSIDTAKNTFYLVLISENFVEACAALSHSATNLPQTQSPVKVDRATVAPSIPVTGQPPAPVSSANGPIQNRQPVSVGPVPTATVKVEPSTVTSMAPVPSFPHIPAVARPATQAIPSIQTSSASPVSQDMVSNAENAPDIKPVVVSGMTPPLRTGPPGGANVNLLNNLSQVRQVMSSAALAGAASSVGQSAVAMHMSNMISTGMATSLPPSQTVFSTGQQGITSMAGSGALMGSAQTGQSPGPNNAFSPQTTSNVASNLGVSQPMQGMNQGSHSGAMMQGGISMNQNMMSGLGQGNVSSGTGGMMPTPGVGQQAQSGIQQLGGSNSSAPNMQLSQPSSGAMQTSQSKYVKVWEGNLSGQRQGQPVLITRLEGYRSASASDSLAANWPPTMQIVRLISQDHMNNKQYVGKADFLVFRAMSQHGFLGQLQDKKLCAVIQLPSQTLLLSVSDKACRLIGMLFPGDMVVFKPQIPNQQQQQQQQLHQQQQQQQQIQQQQQQQQHLQQQQMPQLQQQQQQHQQQQQQQHQLSQLQHHQQQQQQQQQQQQQHQLTQLQHHHQQQQQASPLNQMQQQTSPLNQMQQQTSPLNQMQQQQQPQQMVMGGQAFAQAPGRSQQGGGGGQPNMPGAGFMG

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
583	Phosphorylation	TRLEGYRSASASDSL EEEECCCCCCCCHHH	20.35	23776212
585	Phosphorylation	LEGYRSASASDSLAA EECCCCCCCCHHHHH	29.56	23776212
587	Phosphorylation	GYRSASASDSLAANW CCCCCCCCHHHHHCC	25.82	23776212
589	Phosphorylation	RSASASDSLAANWPP CCCCCCHHHHHCCCC	19.88	23776212
597	Phosphorylation	LAANWPPTMQIVRLI HHHCCCCCHHHHHHH	20.03	23776212

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of MED25_ARATH !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of MED25_ARATH !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of MED25_ARATH !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
DI197_ARATH	HRB1	genetic	17916114
RAP22_ARATH	RAP2.2	physical	21343311
ERF95_ARATH	AT3G23220	physical	21343311
ERF98_ARATH	AT3G23230	physical	21343311
ERF92_ARATH	ERF1	physical	21343311
ERF91_ARATH	AT4G18450	physical	21343311
EF109_ARATH	RRTF1	physical	21343311
PHL1_ARATH	AT5G29000	physical	21343311
BBX20_ARATH	AT4G39070	physical	21343311
ERF92_ARATH	ERF1	physical	22822211
MYC2_ARATH	MYC2	physical	22822211
ERF94_ARATH	ORA59	physical	22822211
WRK10_ARATH	WRKY10	physical	22822211
MY104_ARATH	MYB104	physical	22822211
ERF93_ARATH	ERF15	physical	22822211
ERF98_ARATH	AT3G23230	physical	22822211
MYC4_ARATH	AT4G17880	physical	22822211
BBX20_ARATH	AT4G39070	physical	22822211
DRE2A_ARATH	DREB2A	physical	22822211
MYC3_ARATH	AT5G46760	physical	22822211
MED25_ARATH	PFT1	physical	22822211
MYC2_ARATH	MYC2	physical	22822206
ABI5_ARATH	ABI5	physical	22822206
CDKE1_ARATH	CDKE;1	physical	25281690
VP16_HHV11	UL48	physical	24874105
DRE2A_ARATH	DREB2A	physical	24874105

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.