dbPTM

KAISO_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	KAISO_HUMAN
UniProt AC	Q86T24
Protein Name	Transcriptional regulator Kaiso
Gene Name	ZBTB33
Organism	Homo sapiens (Human).
Sequence Length	672
Subcellular Localization	Nucleus . Cytoplasm . Also cytoplasmic in cells grown at high densities.
Protein Description	Transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3' also known as the consensus kaiso binding site (KBS). Recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. May contribute to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2. Represses expression of MMP7 in conjunction with transcriptional corepressors CBFA2T3, CBFA2T2 and RUNX1T1. [PubMed: 23251453]
Protein Sequence	MESRKLISATDIQYSGSLLNSLNEQRGHGLFCDVTVIVEDRKFRAHKNILSASSTYFHQLFSVAGQVVELSFIRAEIFAEILNYIYSSKIVRVRSDLLDELIKSGQLLGVKFIAELGVPLSQVKSISGTAQDGNTEPLPPDSGDKNLVIQKSKDEAQDNGATIMPIITESFSLSAEDYEMKKIIVTDSDDDDDDVIFCSEILPTKETLPSNNTVAQVQSNPGPVAISDVAPSASNNSPPLTNITPTQKLPTPVNQATLSQTQGSEKLLVSSAPTHLTPNIILLNQTPLSTPPNVSSSLPNHMPSSINLLVQNQQTPNSAILTGNKANEEEEEEIIDDDDDTISSSPDSAVSNTSLVPQADTSQNTSFDGSLIQKMQIPTLLQEPLSNSLKISDIITRNTNDPGVGSKHLMEGQKIITLDTATEIEGLSTGCKVYANIGEDTYDIVIPVKDDPDEGEARLENEIPKTSGSEMANKRMKVKHDDHYELIVDGRVYYICIVCKRSYVCLTSLRRHFNIHSWEKKYPCRYCEKVFPLAEYRTKHEIHHTGERRYQCLACGKSFINYQFMSSHIKSVHSQDPSGDSKLYRLHPCRSLQIRQYAYLSDRSSTIPAMKDDGIGYKVDTGKEPPVGTTTSTQNKPMTWEDIFIQQENDSIFKQNVTDGSTEFEFIIPESY

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
5	Sumoylation	---MESRKLISATDI ---CCCCCEEECCCC	60.49	-
5	Sumoylation	---MESRKLISATDI ---CCCCCEEECCCC	60.49	-
5	Ubiquitination	---MESRKLISATDI ---CCCCCEEECCCC	60.49	-
8	Phosphorylation	MESRKLISATDIQYS CCCCCEEECCCCEEC	35.24	29978859
10	Phosphorylation	SRKLISATDIQYSGS CCCEEECCCCEECHH	27.18	29978859
14	Phosphorylation	ISATDIQYSGSLLNS EECCCCEECHHHHHH	18.48	29978859
15	Phosphorylation	SATDIQYSGSLLNSL ECCCCEECHHHHHHH	12.91	29978859
17	Phosphorylation	TDIQYSGSLLNSLNE CCCEECHHHHHHHHH	24.60	29978859
21	Phosphorylation	YSGSLLNSLNEQRGH ECHHHHHHHHHHCCC	32.32	29978859
84	Phosphorylation	IFAEILNYIYSSKIV HHHHHHHHHHCCCCE	9.59	23401153
87	Phosphorylation	EILNYIYSSKIVRVR HHHHHHHCCCCEEHH	18.65	23401153
103	Ubiquitination	DLLDELIKSGQLLGV HHHHHHHHHCCCCCC	62.25	21906983
124	Ubiquitination	GVPLSQVKSISGTAQ CCCHHHEEEEECCCC	34.14	21906983
145	Acetylation	LPPDSGDKNLVIQKS CCCCCCCCCEEEEEC	56.56	26051181
145	Ubiquitination	LPPDSGDKNLVIQKS CCCCCCCCCEEEEEC	56.56	29967540
151	Sumoylation	DKNLVIQKSKDEAQD CCCEEEEECHHHHHH	48.63	-
151	Sumoylation	DKNLVIQKSKDEAQD CCCEEEEECHHHHHH	48.63	28112733
151	Ubiquitination	DKNLVIQKSKDEAQD CCCEEEEECHHHHHH	48.63	22817900
153	Sumoylation	NLVIQKSKDEAQDNG CEEEEECHHHHHHCC	67.63	28112733
153	Ubiquitination	NLVIQKSKDEAQDNG CEEEEECHHHHHHCC	67.63	21906983
186	Phosphorylation	EMKKIIVTDSDDDDD CCCEEEECCCCCCCC	21.27	30266825
188	Phosphorylation	KKIIVTDSDDDDDDV CEEEECCCCCCCCCE	33.31	26657352
199	Phosphorylation	DDDVIFCSEILPTKE CCCEEEEECCCCCCC	19.54	27251275
204	Phosphorylation	FCSEILPTKETLPSN EEECCCCCCCCCCCC	37.76	27251275
207	Phosphorylation	EILPTKETLPSNNTV CCCCCCCCCCCCCCE	45.59	26074081
210	Phosphorylation	PTKETLPSNNTVAQV CCCCCCCCCCCEEEE	45.91	26074081
213	Phosphorylation	ETLPSNNTVAQVQSN CCCCCCCCEEEECCC	22.82	26074081
219	Phosphorylation	NTVAQVQSNPGPVAI CCEEEECCCCCCEEE	46.25	30108239
227	Phosphorylation	NPGPVAISDVAPSAS CCCCEEEEECCCCCC	19.12	30108239
232	Phosphorylation	AISDVAPSASNNSPP EEEECCCCCCCCCCC	34.29	30108239
234	Phosphorylation	SDVAPSASNNSPPLT EECCCCCCCCCCCCC	40.66	30108239
237	Phosphorylation	APSASNNSPPLTNIT CCCCCCCCCCCCCCC	31.95	25159151
241	Phosphorylation	SNNSPPLTNITPTQK CCCCCCCCCCCCCCC	30.28	30108239
244	Phosphorylation	SPPLTNITPTQKLPT CCCCCCCCCCCCCCC	23.50	30108239
246	Phosphorylation	PLTNITPTQKLPTPV CCCCCCCCCCCCCCC	29.68	25850435
251	Phosphorylation	TPTQKLPTPVNQATL CCCCCCCCCCCCCCH	50.75	15548582
257	O-linked_Glycosylation	PTPVNQATLSQTQGS CCCCCCCCHHCCCCC	19.71	30379171
257	Phosphorylation	PTPVNQATLSQTQGS CCCCCCCCHHCCCCC	19.71	30576142
259	Phosphorylation	PVNQATLSQTQGSEK CCCCCCHHCCCCCCE	27.16	25159151
264	Phosphorylation	TLSQTQGSEKLLVSS CHHCCCCCCEEEEEC	23.03	25159151
379	Phosphorylation	IQKMQIPTLLQEPLS HHHCCCCHHHHCCCC	41.55	22210691
386	Phosphorylation	TLLQEPLSNSLKISD HHHHCCCCCCCCHHH	34.78	28348404
388	Phosphorylation	LQEPLSNSLKISDII HHCCCCCCCCHHHHH	27.88	28348404
390	Sumoylation	EPLSNSLKISDIITR CCCCCCCCHHHHHCC	39.73	28112733
406	Phosphorylation	TNDPGVGSKHLMEGQ CCCCCCCCHHHHCCC	17.94	26714015
407	Sumoylation	NDPGVGSKHLMEGQK CCCCCCCHHHHCCCE	34.52	-
407	Acetylation	NDPGVGSKHLMEGQK CCCCCCCHHHHCCCE	34.52	26051181
407	Sumoylation	NDPGVGSKHLMEGQK CCCCCCCHHHHCCCE	34.52	28112733
407	Ubiquitination	NDPGVGSKHLMEGQK CCCCCCCHHHHCCCE	34.52	21963094
414	Sumoylation	KHLMEGQKIITLDTA HHHHCCCEEEEECCC	47.10	28112733
414	Ubiquitination	KHLMEGQKIITLDTA HHHHCCCEEEEECCC	47.10	-
428	Phosphorylation	ATEIEGLSTGCKVYA CHHHCCCCCCCEEEE	33.34	-
434	Phosphorylation	LSTGCKVYANIGEDT CCCCCEEEEECCCCE	4.57	27642862
441	Phosphorylation	YANIGEDTYDIVIPV EEECCCCEEEEEEEE	20.84	28796482
442	Phosphorylation	ANIGEDTYDIVIPVK EECCCCEEEEEEEEC	18.92	28796482
449	Sumoylation	YDIVIPVKDDPDEGE EEEEEEECCCCCCCH	51.57	-
449	Sumoylation	YDIVIPVKDDPDEGE EEEEEEECCCCCCCH	51.57	28112733
449	Ubiquitination	YDIVIPVKDDPDEGE EEEEEEECCCCCCCH	51.57	29967540
465	Sumoylation	RLENEIPKTSGSEMA HHHCCCCCCCCHHHH	62.07	28112733
465	Ubiquitination	RLENEIPKTSGSEMA HHHCCCCCCCCHHHH	62.07	29967540
474	Sumoylation	SGSEMANKRMKVKHD CCHHHHHHCCCCCCC	44.31	-
474	Acetylation	SGSEMANKRMKVKHD CCHHHHHHCCCCCCC	44.31	25953088
474	Sumoylation	SGSEMANKRMKVKHD CCHHHHHHCCCCCCC	44.31	28112733
474	Ubiquitination	SGSEMANKRMKVKHD CCHHHHHHCCCCCCC	44.31	21906983
475	Ubiquitination	GSEMANKRMKVKHDD CHHHHHHCCCCCCCC	29.93	21890473
479	Sumoylation	ANKRMKVKHDDHYEL HHHCCCCCCCCCEEE	35.48	-
479	Sumoylation	ANKRMKVKHDDHYEL HHHCCCCCCCCCEEE	35.48	28112733
479	Ubiquitination	ANKRMKVKHDDHYEL HHHCCCCCCCCCEEE	35.48	33845483
484	Phosphorylation	KVKHDDHYELIVDGR CCCCCCCEEEEECCE	21.30	20393185
491	Methylation	YELIVDGRVYYICIV EEEEECCEEEEEEEE	14.59	-
502	O-linked_Glycosylation	ICIVCKRSYVCLTSL EEEEECCCEEEHHHH	13.86	30379171
517	Phosphorylation	RRHFNIHSWEKKYPC HHHCCCCCCCCCCCC	32.88	28555341
521	Ubiquitination	NIHSWEKKYPCRYCE CCCCCCCCCCCCCCC	42.80	-
529	Sumoylation	YPCRYCEKVFPLAEY CCCCCCCHHEEHHHH	45.69	-
529	Sumoylation	YPCRYCEKVFPLAEY CCCCCCCHHEEHHHH	45.69	-
529	Ubiquitination	YPCRYCEKVFPLAEY CCCCCCCHHEEHHHH	45.69	21987572
530	Ubiquitination	PCRYCEKVFPLAEYR CCCCCCHHEEHHHHC	2.52	21890473
539	Sumoylation	PLAEYRTKHEIHHTG EHHHHCCCCCCCCCC	30.18	28112733
539	Ubiquitination	PLAEYRTKHEIHHTG EHHHHCCCCCCCCCC	30.18	29967540
558	Phosphorylation	QCLACGKSFINYQFM EEECCCCCHHHHHHH	19.35	21659604
562	Phosphorylation	CGKSFINYQFMSSHI CCCCHHHHHHHHHHH	9.79	21659604
567	Phosphorylation	INYQFMSSHIKSVHS HHHHHHHHHHHHHCC	19.90	21659604
570	Sumoylation	QFMSSHIKSVHSQDP HHHHHHHHHHCCCCC	40.79	28112733
570	Ubiquitination	QFMSSHIKSVHSQDP HHHHHHHHHHCCCCC	40.79	29967540
571	Phosphorylation	FMSSHIKSVHSQDPS HHHHHHHHHCCCCCC	25.18	28985074
574	Phosphorylation	SHIKSVHSQDPSGDS HHHHHHCCCCCCCCC	33.84	29396449
582	Sumoylation	QDPSGDSKLYRLHPC CCCCCCCCEEEEEEC	55.03	-
582	Sumoylation	QDPSGDSKLYRLHPC CCCCCCCCEEEEEEC	55.03	28112733
582	Ubiquitination	QDPSGDSKLYRLHPC CCCCCCCCEEEEEEC	55.03	21906983
583	Ubiquitination	DPSGDSKLYRLHPCR CCCCCCCEEEEEECC	3.31	21890473
599	Phosphorylation	LQIRQYAYLSDRSST EEEEEEEECCCCCCC	11.21	29052541
601	Phosphorylation	IRQYAYLSDRSSTIP EEEEEECCCCCCCCC	20.50	29052541
604	Phosphorylation	YAYLSDRSSTIPAMK EEECCCCCCCCCCCC	36.50	29759185
605	Phosphorylation	AYLSDRSSTIPAMKD EECCCCCCCCCCCCC	31.11	29759185
606	Phosphorylation	YLSDRSSTIPAMKDD ECCCCCCCCCCCCCC	32.02	29759185
611	Sumoylation	SSTIPAMKDDGIGYK CCCCCCCCCCCCCEE	55.57	-
611	Sumoylation	SSTIPAMKDDGIGYK CCCCCCCCCCCCCEE	55.57	28112733
611	Ubiquitination	SSTIPAMKDDGIGYK CCCCCCCCCCCCCEE	55.57	-
618	Sumoylation	KDDGIGYKVDTGKEP CCCCCCEECCCCCCC	28.55	-
618	Sumoylation	KDDGIGYKVDTGKEP CCCCCCEECCCCCCC	28.55	28112733
618	Ubiquitination	KDDGIGYKVDTGKEP CCCCCCEECCCCCCC	28.55	22817900
623	Sumoylation	GYKVDTGKEPPVGTT CEECCCCCCCCCCCC	69.96	-
623	Ubiquitination	GYKVDTGKEPPVGTT CEECCCCCCCCCCCC	69.96	21963094
624	Ubiquitination	YKVDTGKEPPVGTTT EECCCCCCCCCCCCC	57.06	21890473
630	Phosphorylation	KEPPVGTTTSTQNKP CCCCCCCCCCCCCCC	16.90	-
631	Phosphorylation	EPPVGTTTSTQNKPM CCCCCCCCCCCCCCC	29.61	-
636	Ubiquitination	TTTSTQNKPMTWEDI CCCCCCCCCCCHHHE	26.75	21963094
639	Phosphorylation	STQNKPMTWEDIFIQ CCCCCCCCHHHEEEE	33.94	-
651	Phosphorylation	FIQQENDSIFKQNVT EEEECCCCCEECCCC	41.32	24719451

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of KAISO_HUMAN !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of KAISO_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of KAISO_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
HDAC3_HUMAN	HDAC3	physical	14527417
NCOR1_HUMAN	NCOR1	physical	14527417
CTND2_HUMAN	CTNND2	physical	21070476
CTCF_HUMAN	CTCF	physical	16230345
CTND1_HUMAN	CTNND1	physical	21670201
CTNB1_HUMAN	CTNNB1	physical	21670201
TF7L2_HUMAN	TCF7L2	physical	21670201
IMA1_HUMAN	KPNA2	physical	15564377
CTND1_HUMAN	CTNND1	physical	15564377
P53_HUMAN	TP53	physical	25288747
KAT5_HUMAN	KAT5	physical	25288747
EP300_HUMAN	EP300	physical	25288747
CTND1_HUMAN	CTNND1	physical	24498333
FOXM1_HUMAN	FOXM1	physical	26496610
SF3B1_HUMAN	SF3B1	physical	26496610
CLP1L_HUMAN	CLPTM1L	physical	26496610
KLF16_HUMAN	KLF16	physical	26496610
DPH7_HUMAN	DPH7	physical	26496610
TM10A_HUMAN	TRMT10A	physical	26496610
SGF29_HUMAN	CCDC101	physical	26496610
TRIMM_HUMAN	TRIML2	physical	26496610
CCD61_HUMAN	CCDC61	physical	26496610
P53_HUMAN	TP53	physical	26183023
TF65_HUMAN	RELA	physical	26183023

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of KAISO_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach."; Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.; Anal. Chem. 81:4493-4501(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-237, AND MASSSPECTROMETRY.	19413330 [Abstract]
"Immunoaffinity profiling of tyrosine phosphorylation in cancercells."; Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H.,Zha X.-M., Polakiewicz R.D., Comb M.J.; Nat. Biotechnol. 23:94-101(2005). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-442, AND MASSSPECTROMETRY.	15592455 [Abstract]