dbPTM

BTF3_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	BTF3_HUMAN
UniProt AC	P20290
Protein Name	Transcription factor BTF3
Gene Name	BTF3
Organism	Homo sapiens (Human).
Sequence Length	206
Subcellular Localization	Cytoplasm . Nucleus . The heterodimer with NACA is cytoplasmic.
Protein Description	When associated with NACA, prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. BTF3 is also a general transcription factor that can form a stable complex with RNA polymerase II. Required for the initiation of transcription..
Protein Sequence	MRRTGAPAQADSRGRGRARGGCPGGEATLSQPPPRGGTRGQEPQMKETIMNQEKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQFSLKKLGVNNISGIEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEMLPSILNQLGADSLTSLRRLAEALPKQSVDGKAPLATGEDDDDEVPDLVENFDEASKNEAN

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Ubiquitination	------MRRTGAPAQ ------CCCCCCCCC	44.88	29967540
4 (in isoform 2)	Phosphorylation	-	27.59	29507054
4	Phosphorylation	----MRRTGAPAQAD ----CCCCCCCCCCC	27.59	21406692
10 (in isoform 2)	Ubiquitination	-	20.89	21890473
10	Ubiquitination	RTGAPAQADSRGRGR CCCCCCCCCCCCCCC	20.89	23000965
12	Phosphorylation	GAPAQADSRGRGRAR CCCCCCCCCCCCCCC	39.38	21406692
13	Methylation	APAQADSRGRGRARG CCCCCCCCCCCCCCC	39.10	-
13 (in isoform 2)	Ubiquitination	-	39.10	-
13	Dimethylation	APAQADSRGRGRARG CCCCCCCCCCCCCCC	39.10	-
13	Ubiquitination	APAQADSRGRGRARG CCCCCCCCCCCCCCC	39.10	23000965
15	Dimethylation	AQADSRGRGRARGGC CCCCCCCCCCCCCCC	30.25	-
15	Methylation	AQADSRGRGRARGGC CCCCCCCCCCCCCCC	30.25	-
17	Methylation	ADSRGRGRARGGCPG CCCCCCCCCCCCCCC	21.54	-
19	Methylation	SRGRGRARGGCPGGE CCCCCCCCCCCCCCC	40.45	24129315
23	Ubiquitination	GRARGGCPGGEATLS CCCCCCCCCCCCCCC	58.04	22053931
28	Phosphorylation	GCPGGEATLSQPPPR CCCCCCCCCCCCCCC	23.73	29255136
30	Phosphorylation	PGGEATLSQPPPRGG CCCCCCCCCCCCCCC	35.96	23401153
31	Ubiquitination	GGEATLSQPPPRGGT CCCCCCCCCCCCCCC	58.60	24816145
35	Methylation	TLSQPPPRGGTRGQE CCCCCCCCCCCCCCC	63.04	-
38	Phosphorylation	QPPPRGGTRGQEPQM CCCCCCCCCCCCHHH	34.16	28985074
39	Methylation	PPPRGGTRGQEPQMK CCCCCCCCCCCHHHH	48.34	-
42	Ubiquitination	RGGTRGQEPQMKETI CCCCCCCCHHHHHHH	40.08	29967540
43	Ubiquitination	GGTRGQEPQMKETIM CCCCCCCHHHHHHHH	31.51	33845483
46	Ubiquitination	RGQEPQMKETIMNQE CCCCHHHHHHHHCHH	46.59	32015554
46	Acetylation	RGQEPQMKETIMNQE CCCCHHHHHHHHCHH	46.59	25953088
46	Methylation	RGQEPQMKETIMNQE CCCCHHHHHHHHCHH	46.59	24129315
48	Phosphorylation	QEPQMKETIMNQEKL CCHHHHHHHHCHHHH	21.73	20068231
49	Ubiquitination	EPQMKETIMNQEKLA CHHHHHHHHCHHHHH	2.27	27667366
49 (in isoform 2)	Ubiquitination	-	2.27	-
50 (in isoform 2)	Ubiquitination	-	5.75	21890473
50	Ubiquitination	PQMKETIMNQEKLAK HHHHHHHHCHHHHHH	5.75	21890473
50	Sulfoxidation	PQMKETIMNQEKLAK HHHHHHHHCHHHHHH	5.75	30846556
54	2-Hydroxyisobutyrylation	ETIMNQEKLAKLQAQ HHHHCHHHHHHHHHH	43.94	-
54	Ubiquitination	ETIMNQEKLAKLQAQ HHHHCHHHHHHHHHH	43.94	23000965
54 (in isoform 1)	Ubiquitination	-	43.94	21890473
54	Methylation	ETIMNQEKLAKLQAQ HHHHCHHHHHHHHHH	43.94	24129315
54	Acetylation	ETIMNQEKLAKLQAQ HHHHCHHHHHHHHHH	43.94	26051181
57 (in isoform 2)	Phosphorylation	-	52.12	-
57 (in isoform 1)	Ubiquitination	-	52.12	21890473
57	2-Hydroxyisobutyrylation	MNQEKLAKLQAQVRI HCHHHHHHHHHHHCC	52.12	-
57	Acetylation	MNQEKLAKLQAQVRI HCHHHHHHHHHHHCC	52.12	25953088
57	Ubiquitination	MNQEKLAKLQAQVRI HCHHHHHHHHHHHCC	52.12	23000965
67 (in isoform 1)	Ubiquitination	-	48.12	21890473
67	Ubiquitination	AQVRIGGKGTARRKK HHHCCCCCCHHHHCC	48.12	22053931
67	Acetylation	AQVRIGGKGTARRKK HHHCCCCCCHHHHCC	48.12	21669532
67	Methylation	AQVRIGGKGTARRKK HHHCCCCCCHHHHCC	48.12	-
69	Phosphorylation	VRIGGKGTARRKKKV HCCCCCCHHHHCCEE	22.07	22496350
75	Ubiquitination	GTARRKKKVVHRTAT CHHHHCCEEEEEECC	53.15	24816145
80	Phosphorylation	KKKVVHRTATADDKK CCEEEEEECCCCHHH	17.30	22496350
82	Phosphorylation	KVVHRTATADDKKLQ EEEEEECCCCHHHHH	29.97	23401153
86	Ubiquitination	RTATADDKKLQFSLK EECCCCHHHHHHHHH	56.80	29967540
87	Ubiquitination	TATADDKKLQFSLKK ECCCCHHHHHHHHHH	55.29	33845483
91	Phosphorylation	DDKKLQFSLKKLGVN CHHHHHHHHHHHCCC	26.73	23927012
93	Acetylation	KKLQFSLKKLGVNNI HHHHHHHHHHCCCCC	44.76	25953088
93	Ubiquitination	KKLQFSLKKLGVNNI HHHHHHHHHHCCCCC	44.76	27667366
94 (in isoform 1)	Ubiquitination	-	56.62	21890473
94	Ubiquitination	KLQFSLKKLGVNNIS HHHHHHHHHCCCCCC	56.62	22817900
101	Phosphorylation	KLGVNNISGIEEVNM HHCCCCCCCCEEEEE	35.52	20068231
110	Phosphorylation	IEEVNMFTNQGTVIH CEEEEECCCCCEEEE	18.83	24425749
114	Phosphorylation	NMFTNQGTVIHFNNP EECCCCCEEEEECCH	13.41	20068231
116 (in isoform 2)	Phosphorylation	-	2.85	-
127	Ubiquitination	NPKVQASLAANTFTI CHHHEEEEECCEEEE	6.14	23000965
127 (in isoform 2)	Ubiquitination	-	6.14	21890473
133	Ubiquitination	SLAANTFTITGHAET EEECCEEEEECCHHH	18.65	23000965
133 (in isoform 2)	Ubiquitination	-	18.65	21890473
144	Phosphorylation	HAETKQLTEMLPSIL CHHHHHHHHHHHHHH	19.49	28450419
146	Sulfoxidation	ETKQLTEMLPSILNQ HHHHHHHHHHHHHHH	5.85	28183972
149	Phosphorylation	QLTEMLPSILNQLGA HHHHHHHHHHHHHCC	35.43	28450419
157 (in isoform 2)	Phosphorylation	-	42.24	-
158	Ubiquitination	LNQLGADSLTSLRRL HHHHCCHHHHHHHHH	32.77	29967540
158 (in isoform 2)	Ubiquitination	-	32.77	21890473
158	Phosphorylation	LNQLGADSLTSLRRL HHHHCCHHHHHHHHH	32.77	25159151
160	Phosphorylation	QLGADSLTSLRRLAE HHCCHHHHHHHHHHH	29.66	30183078
161	Phosphorylation	LGADSLTSLRRLAEA HCCHHHHHHHHHHHH	25.77	22115753
171	Ubiquitination	RLAEALPKQSVDGKA HHHHHCCCCCCCCCC	56.93	23000965
171	Methylation	RLAEALPKQSVDGKA HHHHHCCCCCCCCCC	56.93	-
171	Acetylation	RLAEALPKQSVDGKA HHHHHCCCCCCCCCC	56.93	26051181
171 (in isoform 1)	Ubiquitination	-	56.93	21890473
173	Phosphorylation	AEALPKQSVDGKAPL HHHCCCCCCCCCCCC	28.44	23927012
177	Acetylation	PKQSVDGKAPLATGE CCCCCCCCCCCCCCC	42.22	26051181
177	Ubiquitination	PKQSVDGKAPLATGE CCCCCCCCCCCCCCC	42.22	23000965
177 (in isoform 1)	Ubiquitination	-	42.22	21890473
182	Phosphorylation	DGKAPLATGEDDDDE CCCCCCCCCCCCCCC	49.11	-
201	Phosphorylation	VENFDEASKNEAN-- HHHHHHHHHHCCC--	33.69	25159151
202 (in isoform 1)	Ubiquitination	-	66.89	21890473
202	Ubiquitination	ENFDEASKNEAN--- HHHHHHHHHCCC---	66.89	29967540

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of BTF3_HUMAN !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of BTF3_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of BTF3_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
CSK2B_HUMAN	CSNK2B	physical	10094400
RPB2_HUMAN	POLR2B	physical	2320128
A4_HUMAN	APP	physical	21832049
RL11_HUMAN	RPL11	physical	22939629
NACA2_HUMAN	NACA2	physical	22939629
PDRG1_HUMAN	PDRG1	physical	22939629
RWDD4_HUMAN	RWDD4	physical	22939629
RL29_HUMAN	RPL29	physical	22939629
ESR1_HUMAN	ESR1	physical	18025262
POL_HV1H2	gag-pol	physical	20016921
2AAA_HUMAN	PPP2R1A	physical	22863883
TXLNB_HUMAN	TXLNB	physical	25416956
TXLNA_HUMAN	TXLNA	physical	25416956
NACAM_HUMAN	NACA	physical	26344197
NACA_HUMAN	NACA	physical	26344197
NACA2_HUMAN	NACA2	physical	26344197
SRSF2_HUMAN	SRSF2	physical	26344197

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of BTF3_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"ATM and ATR substrate analysis reveals extensive protein networksresponsive to DNA damage."; Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III,Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N.,Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.; Science 316:1160-1166(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-30, AND MASSSPECTROMETRY.	17525332 [Abstract]