dbPTM

NACA_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	NACA_HUMAN
UniProt AC	Q13765
Protein Name	Nascent polypeptide-associated complex subunit alpha
Gene Name	NACA
Organism	Homo sapiens (Human).
Sequence Length	215
Subcellular Localization	Cytoplasm. Nucleus. The heterodimer is located mainly in the cytosol, and the homodimer in the nucleus.
Protein Description	Prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. Also reduces the inherent affinity of ribosomes for protein translocation sites in the ER membrane (M sites). May act as a specific coactivator for JUN, binding to DNA and stabilizing the interaction of JUN homodimers with target gene promoters..
Protein Sequence	MPGEATETVPATEQELPQPQAETGSGTESDSDESVPELEEQDSTQATTQQAQLAAAAEIDEEPVSKAKQSRSEKKARKAMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKFKVQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAKAVRALKNNSNDIVNAIMELTM

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
6	Phosphorylation	--MPGEATETVPATE --CCCCCCCCCCCCC	28.08	21406692
8	Phosphorylation	MPGEATETVPATEQE CCCCCCCCCCCCCCC	27.69	21406692
12	Phosphorylation	ATETVPATEQELPQP CCCCCCCCCCCCCCC	31.93	21406692
23	Phosphorylation	LPQPQAETGSGTESD CCCCCCCCCCCCCCC	39.40	21406692
25	Phosphorylation	QPQAETGSGTESDSD CCCCCCCCCCCCCCC	50.38	21406692
27	Phosphorylation	QAETGSGTESDSDES CCCCCCCCCCCCCCC	33.73	21406692
29	Phosphorylation	ETGSGTESDSDESVP CCCCCCCCCCCCCCC	42.10	21406692
31	Phosphorylation	GSGTESDSDESVPEL CCCCCCCCCCCCCCH	53.18	21406692
34	Phosphorylation	TESDSDESVPELEEQ CCCCCCCCCCCHHHC	48.22	21406692
43	Phosphorylation	PELEEQDSTQATTQQ CCHHHCCCHHHHHHH	23.32	15299025
44	Phosphorylation	ELEEQDSTQATTQQA CHHHCCCHHHHHHHH	30.43	21406692
47	Phosphorylation	EQDSTQATTQQAQLA HCCCHHHHHHHHHHH	18.47	21406692
48	Phosphorylation	QDSTQATTQQAQLAA CCCHHHHHHHHHHHH	23.00	21406692
65	Phosphorylation	EIDEEPVSKAKQSRS CCCCCHHHHHHHHHH	37.30	21406692
82	Ubiquitination	KARKAMSKLGLRQVT HHHHHHHHHCHHHHC	33.23	21890473
89	Phosphorylation	KLGLRQVTGVTRVTI HHCHHHHCCCEEEEE	20.00	20068231
92	Phosphorylation	LRQVTGVTRVTIRKS HHHHCCCEEEEEECC	22.29	17924679
100	Ubiquitination	RVTIRKSKNILFVIT EEEEECCCCEEEEEE	51.55	21890473
100	Ubiquitination	RVTIRKSKNILFVIT EEEEECCCCEEEEEE	51.55	21890473
108	Succinylation	NILFVITKPDVYKSP CEEEEEECCCCCCCC	28.26	23954790
108	Ubiquitination	NILFVITKPDVYKSP CEEEEEECCCCCCCC	28.26	21890473
108	Ubiquitination	NILFVITKPDVYKSP CEEEEEECCCCCCCC	28.26	21890473
108	Acetylation	NILFVITKPDVYKSP CEEEEEECCCCCCCC	28.26	23954790
112	Phosphorylation	VITKPDVYKSPASDT EEECCCCCCCCCCCE	17.09	20068231
113	Ubiquitination	ITKPDVYKSPASDTY EECCCCCCCCCCCEE	50.22	21890473
113	Ubiquitination	ITKPDVYKSPASDTY EECCCCCCCCCCCEE	50.22	21890473
120	Nitration	KSPASDTYIVFGEAK CCCCCCEEEEEEEEE	10.48	-
127	Ubiquitination	YIVFGEAKIEDLSQQ EEEEEEEEHHHHHHH	42.18	21890473
127	Ubiquitination	YIVFGEAKIEDLSQQ EEEEEEEEHHHHHHH	42.18	21890473
132	Phosphorylation	EAKIEDLSQQAQLAA EEEHHHHHHHHHHHH	32.35	23186163
142	Ubiquitination	AQLAAAEKFKVQGEA HHHHHHHHHCCCCCH	45.84	21890473
142	Ubiquitination	AQLAAAEKFKVQGEA HHHHHHHHHCCCCCH	45.84	21890473
142	Sumoylation	AQLAAAEKFKVQGEA HHHHHHHHHCCCCCH	45.84	28112733
142	Acetylation	AQLAAAEKFKVQGEA HHHHHHHHHCCCCCH	45.84	19608861
151	Phosphorylation	KVQGEAVSNIQENTQ CCCCCHHHCHHHHCC	34.95	21406692
157	Phosphorylation	VSNIQENTQTPTVQE HHCHHHHCCCCCCCC	32.82	21406692
159	Phosphorylation	NIQENTQTPTVQEES CHHHHCCCCCCCCHH	20.91	15005626
161	Phosphorylation	QENTQTPTVQEESEE HHHCCCCCCCCHHHH	37.90	18669648
166	Phosphorylation	TPTVQEESEEEEVDE CCCCCCHHHHHCCCC	49.94	18691976
174	Phosphorylation	EEEEVDETGVEVKDI HHHCCCCCCCCHHHH	41.90	17081983
185	Sulfoxidation	VKDIELVMSQANVSR HHHHHHHHHHHCCHH	3.72	21406390
186	Phosphorylation	KDIELVMSQANVSRA HHHHHHHHHHCCHHH	20.75	17525332
191	Phosphorylation	VMSQANVSRAKAVRA HHHHHCCHHHHHHHH	26.74	20068231
203	Phosphorylation	VRALKNNSNDIVNAI HHHHHCCCHHHHHHH	45.32	20068231
211	Sulfoxidation	NDIVNAIMELTM--- HHHHHHHHHHHC---	2.99	21406390
827	Phosphorylation	------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------		27251275
842	Phosphorylation	---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------		27251275
869	Phosphorylation	------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------		27251275
876	Phosphorylation	-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------		27251275
896	Phosphorylation	---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------		27251275
901	Phosphorylation	--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------		27251275
913	Phosphorylation	--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------		27251275

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
43	S	Phosphorylation	Kinase	ILK	Q13418	PhosphoELM
159	T	Phosphorylation	Kinase	GSK3B	P49841	PhosphoELM

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Reference
43	S	Phosphorylation	15299025
Phosphorylation of Ser-43 by ILK during cell adhesion may promote nuclear localization.
159	T	Phosphorylation	15299025
Phosphorylation of Thr-159 by GSK3B may promote proteasome mediated degradation (By similarity).

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of NACA_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
FADD_HUMAN	FADD	physical	12684039
APLP1_HUMAN	APLP1	physical	16169070
FUND2_HUMAN	FUNDC2	physical	16169070
CC90B_HUMAN	CCDC90B	physical	16169070
IGS21_HUMAN	IGSF21	physical	16169070
ZHX1_HUMAN	ZHX1	physical	16169070
LRIF1_HUMAN	LRIF1	physical	16169070
FOS_HUMAN	FOS	physical	9488446
JUN_HUMAN	JUN	physical	9488446
NACA2_HUMAN	NACA2	physical	20214399
PYC_HUMAN	PC	physical	22939629
2AAA_HUMAN	PPP2R1A	physical	22863883
BTF3_HUMAN	BTF3	physical	10982809
EF1G_HUMAN	EEF1G	physical	26344197
BTF3_HUMAN	BTF3	physical	21203952

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of NACA_HUMAN

Related Literatures of Post-Translational Modification

Acetylation
Reference	PubMed
"Lysine acetylation targets protein complexes and co-regulates majorcellular functions."; Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.,Olsen J.V., Mann M.; Science 325:834-840(2009). Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-108 AND LYS-142, AND MASSSPECTROMETRY.	19608861 [Abstract]
Phosphorylation
Reference	PubMed
"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions."; Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.; Sci. Signal. 2:RA46-RA46(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-166, AND MASSSPECTROMETRY.	19690332 [Abstract]
"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach."; Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.; Anal. Chem. 81:4493-4501(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-161 AND SER-166, ANDMASS SPECTROMETRY.	19413330 [Abstract]
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-161; SER-166 ANDSER-191, AND MASS SPECTROMETRY.	18669648 [Abstract]
"Kinase-selective enrichment enables quantitative phosphoproteomics ofthe kinome across the cell cycle."; Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R.,Greff Z., Keri G., Stemmann O., Mann M.; Mol. Cell 31:438-448(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-166, AND MASSSPECTROMETRY.	18691976 [Abstract]
"ATM and ATR substrate analysis reveals extensive protein networksresponsive to DNA damage."; Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III,Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N.,Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.; Science 316:1160-1166(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-186, AND MASSSPECTROMETRY.	17525332 [Abstract]
"Global proteomic profiling of phosphopeptides using electron transferdissociation tandem mass spectrometry."; Molina H., Horn D.M., Tang N., Mathivanan S., Pandey A.; Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-166, AND MASSSPECTROMETRY.	17287340 [Abstract]
"Improved titanium dioxide enrichment of phosphopeptides from HeLacells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra."; Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D.; J. Proteome Res. 6:4150-4162(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-92, AND MASSSPECTROMETRY.	17924679 [Abstract]

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