PIF1_ARATH - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: PIF1_ARATH
• UniProt AC: Q8GZM7
• Protein Name: Transcription factor PIF1
• Gene Name: PIF1
• Organism: Arabidopsis thaliana (Mouse-ear cress).
• Sequence Length: 478
• Subcellular Localization: Nucleus .
• Protein Description: Transcription activator. Regulates negatively chlorophyll biosynthesis and seed germination in the dark, and lightinduced degradation of PIF1 relieves this negative regulation to promote photomorphogenesis. Binds to the G-box motif (5'-CACGTG-3') found in many light-regulated promoters. Promotes the expression of SOM, and thus modulates responses to abscisic acid (ABA) and gibberellic acid (GA)..
• Protein Sequence: MHHFVPDFDTDDDYVNNHNSSLNHLPRKSITTMGEDDDLMELLWQNGQVVVQNQRLHTKKPSSSPPKLLPSMDPQQQPSSDQNLFIQEDEMTSWLHYPLRDDDFCSDLLFSAAPTATATATVSQVTAARPPVSSTNESRPPVRNFMNFSRLRGDFNNGRGGESGPLLSKAVVRESTQVSPSATPSAAASESGLTRRTDGTDSSAVAGGGAYNRKGKAVAMTAPAIEITGTSSSVVSKSEIEPEKTNVDDRKRKEREATTTDETESRSEETKQARVSTTSTKRSRAAEVHNLSERKRRDRINERMKALQELIPRCNKSDKASMLDEAIEYMKSLQLQIQMMSMGCGMMPMMYPGMQQYMPHMAMGMGMNQPIPPPSFMPFPNMLAAQRPLPTQTHMAGSGPQYPVHASDPSRVFVPNQQYDPTSGQPQYPAGYTDPYQQFRGLHPTQPPQFQNQATSYPSSSRVSSSKESEDHGNHTTG

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
10	Phosphorylation	HFVPDFDTDDDYVNN CCCCCCCCCCHHHHC	41.12	21330376
64	Phosphorylation	HTKKPSSSPPKLLPS CCCCCCCCCCCCCCC	49.94	19880383
197	Phosphorylation	ESGLTRRTDGTDSSA HCCCCCCCCCCCCCC	35.17	21330376
202	Phosphorylation	RRTDGTDSSAVAGGG CCCCCCCCCCCCCCC	21.75	21330376
464	Phosphorylation	YPSSSRVSSSKESED CCCCCCCCCCCCCCC	27.99	21330376
465	Phosphorylation	PSSSRVSSSKESEDH CCCCCCCCCCCCCCC	42.44	21330376
466	Phosphorylation	SSSRVSSSKESEDHG CCCCCCCCCCCCCCC	32.70	21330376
469	Phosphorylation	RVSSSKESEDHGNHT CCCCCCCCCCCCCCC	52.65	18539749

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
10	T	Phosphorylation	Kinase	CK2	-	Uniprot
197	T	Phosphorylation	Kinase	CK2	-	Uniprot
202	S	Phosphorylation	Kinase	CK2	-	Uniprot
464	S	Phosphorylation	Kinase	CK2	-	Uniprot
465	S	Phosphorylation	Kinase	CK2	-	Uniprot
466	S	Phosphorylation	Kinase	CK2	-	Uniprot
469	S	Phosphorylation	Kinase	CK2	-	Uniprot
-	K	Ubiquitination	E3 ubiquitin ligase	COP1	P43254	PMID:26037329

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
10	T	Phosphorylation	Phosphorylated at Thr-10, Thr-197, Ser-202, Ser-464, Ser-465, Ser-466 and Ser-469 by CK2.	21330376
197	T	Phosphorylation	Phosphorylated at Thr-10, Thr-197, Ser-202, Ser-464, Ser-465, Ser-466 and Ser-469 by CK2.	21330376
202	S	Phosphorylation	Phosphorylated at Thr-10, Thr-197, Ser-202, Ser-464, Ser-465, Ser-466 and Ser-469 by CK2.	21330376
464	S	Phosphorylation	Phosphorylated at Thr-10, Thr-197, Ser-202, Ser-464, Ser-465, Ser-466 and Ser-469 by CK2.	21330376
465	S	Phosphorylation	Phosphorylated at Thr-10, Thr-197, Ser-202, Ser-464, Ser-465, Ser-466 and Ser-469 by CK2.	21330376
466	S	Phosphorylation	Phosphorylated at Thr-10, Thr-197, Ser-202, Ser-464, Ser-465, Ser-466 and Ser-469 by CK2.	21330376
469	S	Phosphorylation	Phosphorylated at Thr-10, Thr-197, Ser-202, Ser-464, Ser-465, Ser-466 and Ser-469 by CK2.	21330376

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of PIF1_ARATH !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
PHYA_ARATH	PHYA	physical	15486102
PHYB_ARATH	PHYB	physical	15486102
PIF1_ARATH	PIL5	physical	21928113
PIF3_ARATH	PIF3	physical	21928113
PIF4_ARATH	PIF4	physical	21928113
PIF5_ARATH	PIL6	physical	21928113
HFR1_ARATH	HFR1	physical	21928113
PHYB_ARATH	PHYB	physical	21928113
UBQ10_ARATH	UBQ10	physical	19255368
PHYA_ARATH	PHYA	physical	19255368
PHYB_ARATH	PHYB	physical	19255368
PIF1_ARATH	PIL5	physical	24179122
HFR1_ARATH	HFR1	physical	24179122
DET1_ARATH	DET1	physical	25248553
BZR1_ARATH	BZR1	physical	22820378
COP1_ARATH	COP1	physical	24858936
HY5_ARATH	HY5	physical	24858936
PHYA_ARATH	PHYA	physical	18539749
PHYB_ARATH	PHYB	physical	18539749

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.