dbPTM

MLF3_YEAST - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	MLF3_YEAST
UniProt AC	P32047
Protein Name	Protein MLF3
Gene Name	MLF3
Organism	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
Sequence Length	452
Subcellular Localization	Cytoplasm .
Protein Description
Protein Sequence	MCVYKSNSNNSNPSFIFERTVQEASSNDLFLQPPVSASNTSHSSRSNSFYNLQTISPIPISGSEVRTPSLRKNSNNVSSPLDNVIPTSRSASNSTTSSLAHQEYILNPICNMQNHHHRRRTLENSVAPALDASCSIVNDENTDLSDVDMVYSRRPSSAVSLNMALLARTNSATLPSSESSPASPDLKLSRSHSHSAATRPTLNNINNTGMTTTTSNGEPNSRILRFYSYVDMLNDEKLAQANNTPTSRPPMKSQAYSCPFILKRSPPQAYSSSSATTTFSNPFIKTTELPATSPYVSPQQSARQYSNNANNNAKSPKNRSSSILFQRQSILSNVDPVANMHKNPKFQIESSDSEEEDLTMDMLDPSFPLSSSLRSSANLASNPELATQTPLSTSSSYTAIGKPMPLSTDPSYVSSSNTLSSEHELRVEKVSEVLKKKVSNGGFSTEFNSCDT

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
5	Ubiquitination	---MCVYKSNSNNSN ---CCEEECCCCCCC	23.82	15699485
6	Phosphorylation	--MCVYKSNSNNSNP --CCEEECCCCCCCC	28.01	22890988
8	Phosphorylation	MCVYKSNSNNSNPSF CCEEECCCCCCCCCE	44.47	22369663
11	Phosphorylation	YKSNSNNSNPSFIFE EECCCCCCCCCEEEE	55.13	22369663
14	Phosphorylation	NSNNSNPSFIFERTV CCCCCCCCEEEEEEC	34.91	22369663
26	Phosphorylation	RTVQEASSNDLFLQP EECEEHHCCCCCCCC	41.22	21440633
36	Phosphorylation	LFLQPPVSASNTSHS CCCCCCCCCCCCCCC	31.58	22369663
38	Phosphorylation	LQPPVSASNTSHSSR CCCCCCCCCCCCCCC	32.89	22369663
40	Phosphorylation	PPVSASNTSHSSRSN CCCCCCCCCCCCCCC	25.89	22369663
41	Phosphorylation	PVSASNTSHSSRSNS CCCCCCCCCCCCCCC	26.14	22369663
43	Phosphorylation	SASNTSHSSRSNSFY CCCCCCCCCCCCCEE	27.49	22369663
44	Phosphorylation	ASNTSHSSRSNSFYN CCCCCCCCCCCCEEE	34.01	22369663
46	Phosphorylation	NTSHSSRSNSFYNLQ CCCCCCCCCCEEECC	38.48	22369663
48	Phosphorylation	SHSSRSNSFYNLQTI CCCCCCCCEEECCEE	30.29	22369663
50	Phosphorylation	SSRSNSFYNLQTISP CCCCCCEEECCEEEC	18.13	22369663
54	Phosphorylation	NSFYNLQTISPIPIS CCEEECCEEECCCCC	26.96	19779198
56	Phosphorylation	FYNLQTISPIPISGS EEECCEEECCCCCCC	21.84	25752575
61	Phosphorylation	TISPIPISGSEVRTP EEECCCCCCCCCCCH	30.80	19779198
67	Phosphorylation	ISGSEVRTPSLRKNS CCCCCCCCHHHCCCC	23.54	23749301
69	Phosphorylation	GSEVRTPSLRKNSNN CCCCCCHHHCCCCCC	39.95	28889911
72	Ubiquitination	VRTPSLRKNSNNVSS CCCHHHCCCCCCCCC	70.76	15699485
74	Phosphorylation	TPSLRKNSNNVSSPL CHHHCCCCCCCCCCH	32.58	22369663
78	Phosphorylation	RKNSNNVSSPLDNVI CCCCCCCCCCHHHCC	28.50	22369663
79	Phosphorylation	KNSNNVSSPLDNVIP CCCCCCCCCHHHCCC	25.79	22369663
87	Phosphorylation	PLDNVIPTSRSASNS CHHHCCCCCCCCCCC	26.16	22890988
88	Phosphorylation	LDNVIPTSRSASNST HHHCCCCCCCCCCCC	20.91	22890988
121	Phosphorylation	NHHHRRRTLENSVAP CCHHHHHHHHHCHHH	36.29	21440633
125	Phosphorylation	RRRTLENSVAPALDA HHHHHHHCHHHHHHC	15.41	21440633
133	Phosphorylation	VAPALDASCSIVNDE HHHHHHCCCEEECCC	14.20	19779198
135	Phosphorylation	PALDASCSIVNDENT HHHHCCCEEECCCCC	27.38	19779198
142	Phosphorylation	SIVNDENTDLSDVDM EEECCCCCCHHHCCE	35.95	19779198
145	Phosphorylation	NDENTDLSDVDMVYS CCCCCCHHHCCEEEE	38.10	21440633
152	Phosphorylation	SDVDMVYSRRPSSAV HHCCEEEECCCCCHH	15.48	19779198
156	Phosphorylation	MVYSRRPSSAVSLNM EEEECCCCCHHHHHH	29.17	22369663
157	Phosphorylation	VYSRRPSSAVSLNMA EEECCCCCHHHHHHH	35.06	22369663
160	Phosphorylation	RRPSSAVSLNMALLA CCCCCHHHHHHHHHH	17.47	22369663
169	Phosphorylation	NMALLARTNSATLPS HHHHHHHCCCCCCCC	28.11	22369663
171	Phosphorylation	ALLARTNSATLPSSE HHHHHCCCCCCCCCC	23.24	22369663
173	Phosphorylation	LARTNSATLPSSESS HHHCCCCCCCCCCCC	38.74	22369663
176	Phosphorylation	TNSATLPSSESSPAS CCCCCCCCCCCCCCC	50.26	22890988
177	Phosphorylation	NSATLPSSESSPASP CCCCCCCCCCCCCCC	39.29	22369663
179	Phosphorylation	ATLPSSESSPASPDL CCCCCCCCCCCCCCC	43.50	22369663
180	Phosphorylation	TLPSSESSPASPDLK CCCCCCCCCCCCCCC	22.04	22369663
183	Phosphorylation	SSESSPASPDLKLSR CCCCCCCCCCCCCCC	23.49	22369663
187	Acetylation	SPASPDLKLSRSHSH CCCCCCCCCCCCCCC	52.36	24489116
187	Ubiquitination	SPASPDLKLSRSHSH CCCCCCCCCCCCCCC	52.36	17644757
189	Phosphorylation	ASPDLKLSRSHSHSA CCCCCCCCCCCCCCC	30.10	22369663
191	Phosphorylation	PDLKLSRSHSHSAAT CCCCCCCCCCCCCCC	26.43	22369663
193	Phosphorylation	LKLSRSHSHSAATRP CCCCCCCCCCCCCCC	22.00	22369663
195	Phosphorylation	LSRSHSHSAATRPTL CCCCCCCCCCCCCCC	23.71	22369663
198	Phosphorylation	SHSHSAATRPTLNNI CCCCCCCCCCCCCCC	36.58	22369663
201	Phosphorylation	HSAATRPTLNNINNT CCCCCCCCCCCCCCC	38.54	22369663
208	Phosphorylation	TLNNINNTGMTTTTS CCCCCCCCCCCEECC	24.68	22369663
211	Phosphorylation	NINNTGMTTTTSNGE CCCCCCCCEECCCCC	23.49	22369663
212	Phosphorylation	INNTGMTTTTSNGEP CCCCCCCEECCCCCC	21.13	22369663
213	Phosphorylation	NNTGMTTTTSNGEPN CCCCCCEECCCCCCC	21.04	22369663
214	Phosphorylation	NTGMTTTTSNGEPNS CCCCCEECCCCCCCC	20.36	22369663
215	Phosphorylation	TGMTTTTSNGEPNSR CCCCEECCCCCCCCE	40.52	22369663
221	Phosphorylation	TSNGEPNSRILRFYS CCCCCCCCEEEHHHH	31.26	22369663
227	Phosphorylation	NSRILRFYSYVDMLN CCEEEHHHHHHHHHC	7.83	22369663
228	Phosphorylation	SRILRFYSYVDMLND CEEEHHHHHHHHHCH	18.90	22369663
229	Phosphorylation	RILRFYSYVDMLNDE EEEHHHHHHHHHCHH	6.78	22890988
232	Oxidation	RFYSYVDMLNDEKLA HHHHHHHHHCHHHHH	2.41	15665377
237	Ubiquitination	VDMLNDEKLAQANNT HHHHCHHHHHHHCCC	52.57	17644757
244	Phosphorylation	KLAQANNTPTSRPPM HHHHHCCCCCCCCCC	27.96	22369663
246	Phosphorylation	AQANNTPTSRPPMKS HHHCCCCCCCCCCHH	35.02	24909858
247	Phosphorylation	QANNTPTSRPPMKSQ HHCCCCCCCCCCHHC	43.95	22369663
252	Ubiquitination	PTSRPPMKSQAYSCP CCCCCCCHHCCCCCC	45.23	17644757
253	Phosphorylation	TSRPPMKSQAYSCPF CCCCCCHHCCCCCCE	17.50	21440633
256	Phosphorylation	PPMKSQAYSCPFILK CCCHHCCCCCCEEEE	11.81	22890988
257	Phosphorylation	PMKSQAYSCPFILKR CCHHCCCCCCEEEEC	20.52	22369663
263	Acetylation	YSCPFILKRSPPQAY CCCCEEEECCCCCCC	46.11	24489116
263	Ubiquitination	YSCPFILKRSPPQAY CCCCEEEECCCCCCC	46.11	17644757
265	Phosphorylation	CPFILKRSPPQAYSS CCEEEECCCCCCCCC	39.07	22369663
270	Phosphorylation	KRSPPQAYSSSSATT ECCCCCCCCCCCCCC	12.11	22369663
271	Phosphorylation	RSPPQAYSSSSATTT CCCCCCCCCCCCCCC	27.10	20377248
272	Phosphorylation	SPPQAYSSSSATTTF CCCCCCCCCCCCCCC	18.68	22369663
273	Phosphorylation	PPQAYSSSSATTTFS CCCCCCCCCCCCCCC	20.32	20377248
274	Phosphorylation	PQAYSSSSATTTFSN CCCCCCCCCCCCCCC	31.42	22369663
276	Phosphorylation	AYSSSSATTTFSNPF CCCCCCCCCCCCCCC	28.46	22369663
277	Phosphorylation	YSSSSATTTFSNPFI CCCCCCCCCCCCCCC	26.09	22369663
278	Phosphorylation	SSSSATTTFSNPFIK CCCCCCCCCCCCCCE	22.25	22369663
280	Phosphorylation	SSATTTFSNPFIKTT CCCCCCCCCCCCEEC	41.31	22369663
285	Ubiquitination	TFSNPFIKTTELPAT CCCCCCCEECCCCCC	50.18	17644757
286	Phosphorylation	FSNPFIKTTELPATS CCCCCCEECCCCCCC	22.23	22890988
287	Phosphorylation	SNPFIKTTELPATSP CCCCCEECCCCCCCC	30.44	22890988
292	Phosphorylation	KTTELPATSPYVSPQ EECCCCCCCCCCCHH	28.44	22890988
293	Phosphorylation	TTELPATSPYVSPQQ ECCCCCCCCCCCHHH	19.15	22369663
295	Phosphorylation	ELPATSPYVSPQQSA CCCCCCCCCCHHHHH	17.21	22369663
297	Phosphorylation	PATSPYVSPQQSARQ CCCCCCCCHHHHHHH	15.91	22369663
301	Phosphorylation	PYVSPQQSARQYSNN CCCCHHHHHHHHHCC	21.85	22890988
305	Phosphorylation	PQQSARQYSNNANNN HHHHHHHHHCCCCCC	14.07	22369663
306	Phosphorylation	QQSARQYSNNANNNA HHHHHHHHCCCCCCC	18.44	20377248
314	Ubiquitination	NNANNNAKSPKNRSS CCCCCCCCCCCCHHH	70.21	23749301
315	Phosphorylation	NANNNAKSPKNRSSS CCCCCCCCCCCHHHH	38.40	22369663
320	Phosphorylation	AKSPKNRSSSILFQR CCCCCCHHHHHHHHH	37.52	22369663
321	Phosphorylation	KSPKNRSSSILFQRQ CCCCCHHHHHHHHHH	20.40	22369663
322	Phosphorylation	SPKNRSSSILFQRQS CCCCHHHHHHHHHHH	25.26	22369663
329	Phosphorylation	SILFQRQSILSNVDP HHHHHHHHHHHCCCC	27.90	22369663
332	Phosphorylation	FQRQSILSNVDPVAN HHHHHHHHCCCCCCC	32.89	20377248
342	Ubiquitination	DPVANMHKNPKFQIE CCCCCCCCCCCCEEC	65.26	17644757
350	Phosphorylation	NPKFQIESSDSEEED CCCCEECCCCCCCCC	40.86	19779198
351	Phosphorylation	PKFQIESSDSEEEDL CCCEECCCCCCCCCC	32.16	21440633
353	Phosphorylation	FQIESSDSEEEDLTM CEECCCCCCCCCCCH	49.18	21440633
359	Phosphorylation	DSEEEDLTMDMLDPS CCCCCCCCHHHCCCC	24.36	19779198
375	Phosphorylation	PLSSSLRSSANLASN CCCHHHHHHCCCCCC	38.81	22369663
376	Phosphorylation	LSSSLRSSANLASNP CCHHHHHHCCCCCCH	18.10	22369663
381	Phosphorylation	RSSANLASNPELATQ HHHCCCCCCHHHHHC	56.96	22369663
387	Phosphorylation	ASNPELATQTPLSTS CCCHHHHHCCCCCCC	45.67	22369663
389	Phosphorylation	NPELATQTPLSTSSS CHHHHHCCCCCCCCC	23.11	22369663
392	Phosphorylation	LATQTPLSTSSSYTA HHHCCCCCCCCCCCC	27.70	22369663
393	Phosphorylation	ATQTPLSTSSSYTAI HHCCCCCCCCCCCCC	40.07	22369663
394	Phosphorylation	TQTPLSTSSSYTAIG HCCCCCCCCCCCCCC	17.57	22369663
395	Phosphorylation	QTPLSTSSSYTAIGK CCCCCCCCCCCCCCC	27.70	22369663
396	Phosphorylation	TPLSTSSSYTAIGKP CCCCCCCCCCCCCCC	26.69	22369663
397	Phosphorylation	PLSTSSSYTAIGKPM CCCCCCCCCCCCCCC	11.35	22369663
398	Phosphorylation	LSTSSSYTAIGKPMP CCCCCCCCCCCCCCC	17.47	22369663
407	Phosphorylation	IGKPMPLSTDPSYVS CCCCCCCCCCHHHCC	25.54	22369663
408	Phosphorylation	GKPMPLSTDPSYVSS CCCCCCCCCHHHCCC	60.66	22369663
411	Phosphorylation	MPLSTDPSYVSSSNT CCCCCCHHHCCCCCC	40.76	22369663
412	Phosphorylation	PLSTDPSYVSSSNTL CCCCCHHHCCCCCCC	15.11	22369663
414	Phosphorylation	STDPSYVSSSNTLSS CCCHHHCCCCCCCCC	21.59	22369663
415	Phosphorylation	TDPSYVSSSNTLSSE CCHHHCCCCCCCCCC	19.98	22369663
416	Phosphorylation	DPSYVSSSNTLSSEH CHHHCCCCCCCCCCH	26.74	22369663
418	Phosphorylation	SYVSSSNTLSSEHEL HHCCCCCCCCCCHHH	29.93	22369663
420	Phosphorylation	VSSSNTLSSEHELRV CCCCCCCCCCHHHHH	31.48	22369663
421	Phosphorylation	SSSNTLSSEHELRVE CCCCCCCCCHHHHHH	46.19	22369663
431	Phosphorylation	ELRVEKVSEVLKKKV HHHHHHHHHHHHHHH	32.81	25704821
437	Ubiquitination	VSEVLKKKVSNGGFS HHHHHHHHHHCCCCC	49.67	23749301
439	Phosphorylation	EVLKKKVSNGGFSTE HHHHHHHHCCCCCCC	38.34	22369663
444	Phosphorylation	KVSNGGFSTEFNSCD HHHCCCCCCCCCCCC	30.24	22369663
445	Phosphorylation	VSNGGFSTEFNSCDT HHCCCCCCCCCCCCC	42.19	22369663
452	Phosphorylation	TEFNSCDT------- CCCCCCCC-------	44.85	28889911

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of MLF3_YEAST !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of MLF3_YEAST !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of MLF3_YEAST !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
GIC2_YEAST	GIC2	genetic	16816427
PCL2_YEAST	PCL2	genetic	16816427
CG11_YEAST	CLN1	genetic	16816427
CG12_YEAST	CLN2	genetic	16816427
PCL1_YEAST	PCL1	genetic	16816427
GIC1_YEAST	GIC1	genetic	16816427
CKS1_YEAST	CKS1	genetic	27708008
RPB7_YEAST	RPB7	genetic	27708008
RSP5_YEAST	RSP5	genetic	27708008
SYMC_YEAST	MES1	genetic	27708008
KTHY_YEAST	CDC8	genetic	27708008
CDC11_YEAST	CDC11	genetic	27708008
RPB2_YEAST	RPB2	genetic	27708008
PP2B1_YEAST	CNA1	physical	24930733
PP2B2_YEAST	CMP2	physical	24930733

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of MLF3_YEAST

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"A multidimensional chromatography technology for in-depthphosphoproteome analysis."; Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.; Mol. Cell. Proteomics 7:1389-1396(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-8; SER-11; SER-14;SER-36; SER-38; SER-41; SER-46; SER-48; SER-56; SER-74; SER-78;THR-121; SER-157; THR-169; SER-171; THR-173; SER-177; SER-179;SER-180; SER-183; SER-189; TYR-227; SER-228; SER-257; SER-274;TYR-295; SER-297; SER-320; SER-321; SER-322; SER-381; SER-421; SER-439AND THR-452, AND MASS SPECTROMETRY.	18407956 [Abstract]
"Proteome-wide identification of in vivo targets of DNA damagecheckpoint kinases."; Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.; Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-74; SER-78; SER-156;SER-157; SER-189; SER-320 AND SER-322, AND MASS SPECTROMETRY.	17563356 [Abstract]
"Analysis of phosphorylation sites on proteins from Saccharomycescerevisiae by electron transfer dissociation (ETD) massspectrometry."; Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L.,Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.; Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-320, AND MASSSPECTROMETRY.	17287358 [Abstract]
"Quantitative phosphoproteomics applied to the yeast pheromonesignaling pathway."; Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J.,Mann M., Jensen O.N.; Mol. Cell. Proteomics 4:310-327(2005). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-8; SER-157; SER-160;SER-171; SER-189; SER-228; SER-257; SER-320 AND SER-322, AND MASSSPECTROMETRY.	15665377 [Abstract]
"Phosphoproteome analysis by mass spectrometry and its application toSaccharomyces cerevisiae."; Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M.,Shabanowitz J., Hunt D.F., White F.M.; Nat. Biotechnol. 20:301-305(2002). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-293 AND SER-297, ANDMASS SPECTROMETRY.	11875433 [Abstract]
"Large-scale phosphorylation analysis of alpha-factor-arrestedSaccharomyces cerevisiae."; Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,Elias J.E., Gygi S.P.; J. Proteome Res. 6:1190-1197(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-227, AND MASSSPECTROMETRY.	17330950 [Abstract]