JUN_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: JUN_MOUSE
• UniProt AC: P05627
• Protein Name: Transcription factor AP-1
• Gene Name: Jun
• Organism: Mus musculus (Mouse).
• Sequence Length: 334
• Subcellular Localization: Nucleus .
• Protein Description: Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells. Binds to the USP28 promoter in colorectal cancer (CRC) cells (By similarity)..
• Protein Sequence: MTAKMETTFYDDALNASFLQSESGAYGYSNPKILKQSMTLNLADPVGSLKPHLRAKNSDLLTSPDVGLLKLASPELERLIIQSSNGHITTTPTPTQFLCPKNVTDEQEGFAEGFVRALAELHSQNTLPSVTSAAQPVSGAGMVAPAVASVAGAGGGGGYSASLHSEPPVYANLSNFNPGALSSGGGAPSYGAAGLAFPSQPQQQQQPPQPPHHLPQQIPVQHPRLQALKEEPQTVPEMPGETPPLSPIDMESQERIKAERKRMRNRIAASKCRKRKLERIARLEEKVKTLKAQNSELASTANMLREQVAQLKQKVMNHVNSGCQLMLTQQLQTF

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Phosphorylation	------MTAKMETTF ------CCCCCCCEE	34.68	-
8	Phosphorylation	MTAKMETTFYDDALN CCCCCCCEEHHHHHC	13.59	25777480
10	Phosphorylation	AKMETTFYDDALNAS CCCCCEEHHHHHCHH	15.72	25777480
17	Phosphorylation	YDDALNASFLQSESG HHHHHCHHHHCCCCC	25.97	25777480
21	Phosphorylation	LNASFLQSESGAYGY HCHHHHCCCCCCCCC	35.56	25777480
26	Phosphorylation	LQSESGAYGYSNPKI HCCCCCCCCCCCHHH	21.81	25777480
28	Phosphorylation	SESGAYGYSNPKILK CCCCCCCCCCHHHHH	7.81	25777480
56	Ubiquitination	LKPHLRAKNSDLLTS CCHHHHHCCCCCCCC	51.02	-
56	Acetylation	LKPHLRAKNSDLLTS CCHHHHHCCCCCCCC	51.02	23806337
58	Phosphorylation	PHLRAKNSDLLTSPD HHHHHCCCCCCCCCC	29.00	22942356
62	Phosphorylation	AKNSDLLTSPDVGLL HCCCCCCCCCCCCHH	45.57	25521595
63	Phosphorylation	KNSDLLTSPDVGLLK CCCCCCCCCCCCHHH	21.05	27087446
73	Phosphorylation	VGLLKLASPELERLI CCHHHHCCHHHHHEE	29.37	27087446
83	Phosphorylation	LERLIIQSSNGHITT HHHEEEECCCCCEEC	18.50	25619855
84	Phosphorylation	ERLIIQSSNGHITTT HHEEEECCCCCEECC	30.38	25619855
84	O-linked_Glycosylation	ERLIIQSSNGHITTT HHEEEECCCCCEECC	30.38	30012597
89	Phosphorylation	QSSNGHITTTPTPTQ ECCCCCEECCCCCCE	20.95	-
90	Phosphorylation	SSNGHITTTPTPTQF CCCCCEECCCCCCEE	30.12	25619855
91	Phosphorylation	SNGHITTTPTPTQFL CCCCEECCCCCCEEE	19.26	25619855
93	Phosphorylation	GHITTTPTPTQFLCP CCEECCCCCCEEECC	36.55	25619855
95	Phosphorylation	ITTTPTPTQFLCPKN EECCCCCCEEECCCC	34.14	25619855
131	O-linked_Glycosylation	QNTLPSVTSAAQPVS CCCCCCCCCCCCCCC	19.58	30012597
132	O-linked_Glycosylation	NTLPSVTSAAQPVSG CCCCCCCCCCCCCCC	21.32	30012597
138	O-linked_Glycosylation	TSAAQPVSGAGMVAP CCCCCCCCCCCCCHH	29.61	30012597
229	Sumoylation	HPRLQALKEEPQTVP CHHHHHHHHCCCCCC	64.15	-
229	Sumoylation	HPRLQALKEEPQTVP CHHHHHHHHCCCCCC	64.15	-
234	Phosphorylation	ALKEEPQTVPEMPGE HHHHCCCCCCCCCCC	49.06	25619855
242	Phosphorylation	VPEMPGETPPLSPID CCCCCCCCCCCCCCC	35.80	12881422
246	Phosphorylation	PGETPPLSPIDMESQ CCCCCCCCCCCHHHH	26.11	12881422
252	Phosphorylation	LSPIDMESQERIKAE CCCCCHHHHHHHHHH	30.61	25619855
257	Sumoylation	MESQERIKAERKRMR HHHHHHHHHHHHHHH	50.87	-
257	Sumoylation	MESQERIKAERKRMR HHHHHHHHHHHHHHH	50.87	-
274	Acetylation	IAASKCRKRKLERIA HHHHHHHHHHHHHHH	64.45	-
289	Phosphorylation	RLEEKVKTLKAQNSE HHHHHHHHHHHHCHH	36.16	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
2	T	Phosphorylation	Kinase	PAK2	Q8CIN4	Uniprot
8	T	Phosphorylation	Kinase	PAK2	Q8CIN4	Uniprot
63	S	Phosphorylation	Kinase	MAPK3	Q63844	GPS
63	S	Phosphorylation	Kinase	JNK1	P45983	PSP
63	S	Phosphorylation	Kinase	MAPK8	Q91Y86	GPS
63	S	Phosphorylation	Kinase	JNK2	P45984	PSP
63	S	Phosphorylation	Kinase	MAPK10	Q61831	GPS
73	S	Phosphorylation	Kinase	PLK3	Q60806	Uniprot
73	S	Phosphorylation	Kinase	MAPK3	Q63844	GPS
73	S	Phosphorylation	Kinase	JNK1	Q91Y86	PSP
89	T	Phosphorylation	Kinase	PAK2	Q8CIN4	Uniprot
91	T	Phosphorylation	Kinase	MAPK8	Q91Y86	GPS
93	T	Phosphorylation	Kinase	MAPK8	Q91Y86	GPS
93	T	Phosphorylation	Kinase	PAK2	Q8CIN4	Uniprot
242	T	Phosphorylation	Kinase	MTOR	Q9JLN9	PSP
242	T	Phosphorylation	Kinase	GSK3B	Q9WV60	PSP
246	S	Phosphorylation	Kinase	MTOR	Q9JLN9	PSP
246	S	Phosphorylation	Kinase	MAPK1	P63085	GPS
246	S	Phosphorylation	Kinase	GSK3B	Q9WV60	PSP
252	S	Phosphorylation	Kinase	GSK3-BETA	Q9WV60	Uniprot
289	T	Phosphorylation	Kinase	PAK2	Q8CIN4	Uniprot
-	K	Ubiquitination	E3 ubiquitin ligase	Fbxw7	Q8VBV4	PMID:22199232
-	K	Ubiquitination	E3 ubiquitin ligase	Itch	Q8C863	PMID:22199232
-	K	Ubiquitination	E3 ubiquitin ligase	Map3k1	P53349	PMID:22199232
-	K	Ubiquitination	E3 ubiquitin ligase	Cop1	Q9R1A8	PMID:21403399

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
2	T	Phosphorylation	Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-289 thereby promoting JUN-mediated cell proliferation and transformation.	21183079
8	T	Phosphorylation	Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-289 thereby promoting JUN-mediated cell proliferation and transformation.	-
89	T	Phosphorylation	Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-289 thereby promoting JUN-mediated cell proliferation and transformation.	-
93	T	Phosphorylation	Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-289 thereby promoting JUN-mediated cell proliferation and transformation.	-
242	T	Phosphorylation	Phosphorylated at Thr-242, Ser-246 and Ser-252 by GSK3B; phosphorylation reduces its ability to bind DNA.	21183079
242	T	Phosphorylation	Phosphorylated by DYRK2 at Ser-246; this primes the protein for subsequent phosphorylation by GSK3B at Thr-242.	21183079
246	S	Phosphorylation	Phosphorylated at Thr-242, Ser-246 and Ser-252 by GSK3B; phosphorylation reduces its ability to bind DNA.	21183079
246	S	Phosphorylation	Phosphorylated by DYRK2 at Ser-246; this primes the protein for subsequent phosphorylation by GSK3B at Thr-242.	21183079
252	S	Phosphorylation	Phosphorylated at Thr-242, Ser-246 and Ser-252 by GSK3B; phosphorylation reduces its ability to bind DNA.	-
271	K	Acetylation	Acetylated at Lys-271 by EP300.	-
289	T	Phosphorylation	Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-289 thereby promoting JUN-mediated cell proliferation and transformation.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of JUN_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
SMCA2_MOUSE	Smarca2	physical	11053448
SNF5_MOUSE	Smarcb1	physical	11053448
JUN_MOUSE	Jun	physical	1631061
HHEX_MOUSE	Hhex	physical	11551904
HXC8_MOUSE	Hoxc8	physical	11551904
HXA9_MOUSE	Hoxa9	physical	11551904
HME1_MOUSE	En1	physical	11551904
ITCH_MOUSE	Itch	physical	16901904
ESR1_MOUSE	Esr1	physical	19605792
MAFB_MOUSE	Mafb	physical	20211142
STAT3_MOUSE	Stat3	physical	20211142
ITF2_MOUSE	Tcf4	physical	16007074
CTNB1_MOUSE	Ctnnb1	physical	16007074
TF65_MOUSE	Rela	physical	21262356
SP1_MOUSE	Sp1	physical	21262356
JUN_MOUSE	Jun	physical	21262356
CREB1_MOUSE	Creb1	physical	21262356
FOS_MOUSE	Fos	physical	21478324
CYLD_MOUSE	Cyld	physical	21478324
SMAD3_MOUSE	Smad3	physical	22610349
NUMB_HUMAN	NUMB	physical	9632782
SNPC5_MOUSE	Snapc5	physical	15867431
SIR1_MOUSE	Sirt1	physical	20042607
MAFB_MOUSE	Mafb	physical	12798298
FOS_MOUSE	Fos	physical	12145309
BAG1_MOUSE	Bag1	physical	20516211
SIR1_MOUSE	Sirt1	physical	18676681

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.