dbPTM

MAFB_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	MAFB_MOUSE
UniProt AC	P54841
Protein Name	Transcription factor MafB
Gene Name	Mafb
Organism	Mus musculus (Mouse).
Sequence Length	323
Subcellular Localization	Nucleus .
Protein Description	Acts as a transcriptional activator or repressor. Plays a pivotal role in regulating lineage-specific hematopoiesis by repressing ETS1-mediated transcription of erythroid-specific genes in myeloid cells. Required for monocytic, macrophage, osteoclast, podocyte and islet beta cell differentiation. Involved in renal tubule survival and F4/80 maturation. Activates the insulin and glucagon promoters. Together with PAX6, transactivates weakly the glucagon gene promoter through the G1 element. SUMO modification controls its transcriptional activity and ability to specify macrophage fate. Binds element G1 on the glucagon promoter. Involved either as an oncogene or as a tumor suppressor, depending on the cell context..
Protein Sequence	MAAELSMGQELPTSPLAMEYVNDFDLLKFDVKKEPLGRAERPGRPCTRLQPAGSVSSTPLSTPCSSVPSSPSFSPTEPKTHLEDLYWMASNYQQMNPEALNLTPEDAVEALIGSHPVPQPLQSFDGFRSAHHHHHHHHPHPHHGYPGAGVTHDDLGQHAHPHHHHHHQASPPPSSAASPAQQLPTSHPGPGPHATAAATAAGGNGSVEDRFSDDQLVSMSVRELNRHLRGFTKDEVIRLKQKRRTLKNRGYAQSCRYKRVQQKHHLENEKTQLIQQVEQLKQEVSRLARERDAYKVKCEKLANSGFREAGSTSDSPSSPEFFL

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
13	Phosphorylation	SMGQELPTSPLAMEY CCCCCCCCCCCHHHH	56.45	24719451
14	Phosphorylation	MGQELPTSPLAMEYV CCCCCCCCCCHHHHC	18.90	26824392
32	Sumoylation	DLLKFDVKKEPLGRA HCEECCCCCCCCCCC	53.95	-
32	Sumoylation	DLLKFDVKKEPLGRA HCEECCCCCCCCCCC	53.95	17548468
58	Phosphorylation	PAGSVSSTPLSTPCS CCCCCCCCCCCCCCC	22.27	23984901
61	Phosphorylation	SVSSTPLSTPCSSVP CCCCCCCCCCCCCCC	31.35	20208071
62	Phosphorylation	VSSTPLSTPCSSVPS CCCCCCCCCCCCCCC	35.68	19932079
65	Phosphorylation	TPLSTPCSSVPSSPS CCCCCCCCCCCCCCC	35.26	20208071
66	Phosphorylation	PLSTPCSSVPSSPSF CCCCCCCCCCCCCCC	44.77	20208071
69	Phosphorylation	TPCSSVPSSPSFSPT CCCCCCCCCCCCCCC	53.37	20208071
70	Phosphorylation	PCSSVPSSPSFSPTE CCCCCCCCCCCCCCC	20.63	20208071
72	Phosphorylation	SSVPSSPSFSPTEPK CCCCCCCCCCCCCCC	40.06	20208071
74	Phosphorylation	VPSSPSFSPTEPKTH CCCCCCCCCCCCCCH	34.94	20208071
76	Phosphorylation	SSPSFSPTEPKTHLE CCCCCCCCCCCCHHH	64.82	20208071
103	Phosphorylation	NPEALNLTPEDAVEA CHHHHCCCHHHHHHH	24.45	20208071
114	Phosphorylation	AVEALIGSHPVPQPL HHHHHHCCCCCCCCC	20.02	20208071
123	Phosphorylation	PVPQPLQSFDGFRSA CCCCCCCCCCCCCCC	32.75	20208071
151	Phosphorylation	GYPGAGVTHDDLGQH CCCCCCCCCCCCCCC	20.76	20208071
170	Phosphorylation	HHHHHQASPPPSSAA CCCCCCCCCCCCCCC	31.22	20208071
174	Phosphorylation	HQASPPPSSAASPAQ CCCCCCCCCCCCHHH	38.08	20208071
178	Phosphorylation	PPPSSAASPAQQLPT CCCCCCCCHHHHCCC	22.08	20208071
206	Phosphorylation	TAAGGNGSVEDRFSD HHCCCCCCHHHCCCH	26.74	20208071
212	Phosphorylation	GSVEDRFSDDQLVSM CCHHHCCCHHHHHHH	40.97	20208071
218	Phosphorylation	FSDDQLVSMSVRELN CCHHHHHHHHHHHHH	17.58	22817900
220	Phosphorylation	DDQLVSMSVRELNRH HHHHHHHHHHHHHHH	15.54	22817900
297	Sumoylation	ERDAYKVKCEKLANS HHHHHHHHHHHHHHC	32.31	-
297	Sumoylation	ERDAYKVKCEKLANS HHHHHHHHHHHHHHC	32.31	17548468
304	Phosphorylation	KCEKLANSGFREAGS HHHHHHHCCCCCCCC	33.43	20208071
311	Phosphorylation	SGFREAGSTSDSPSS CCCCCCCCCCCCCCC	31.61	20208071
312	Phosphorylation	GFREAGSTSDSPSSP CCCCCCCCCCCCCCC	35.32	20208071
313	Phosphorylation	FREAGSTSDSPSSPE CCCCCCCCCCCCCCC	37.48	20208071
315	Phosphorylation	EAGSTSDSPSSPEFF CCCCCCCCCCCCCCC	26.84	20208071
317	Phosphorylation	GSTSDSPSSPEFFL- CCCCCCCCCCCCCC-	63.58	20208071
318	Phosphorylation	STSDSPSSPEFFL-- CCCCCCCCCCCCC--	31.65	20208071

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
62	T	Phosphorylation	Kinase	MAPK8	P45983	GPS

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Reference
32	K	Sumoylation	17548468
Sumoylation on Lys-32 and Lys-297 stimulates its transcriptional repression activity and promotes macrophage differentiation from myeloid progenitors.
297	K	Sumoylation	17548468
Sumoylation on Lys-32 and Lys-297 stimulates its transcriptional repression activity and promotes macrophage differentiation from myeloid progenitors.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of MAFB_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
TAF5_MOUSE	Taf5	physical	20211142
JUN_MOUSE	Jun	physical	12798298
SUMO1_HUMAN	SUMO1	physical	17548468

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of MAFB_MOUSE

Related Literatures of Post-Translational Modification

Sumoylation
Reference	PubMed
"SUMO modification regulates MafB-driven macrophage differentiation byenabling Myb-dependent transcriptional repression."; Tillmanns S., Otto C., Jaffray E., Du Roure C., Bakri Y., Vanhille L.,Sarrazin S., Hay R.T., Sieweke M.H.; Mol. Cell. Biol. 27:5554-5564(2007). Cited for: FUNCTION, SUMOYLATION AT LYS-32 AND LYS-297, AND MUTAGENESIS OF LYS-32AND LYS-297.	17548468 [Abstract]

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