EHMT1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: EHMT1_HUMAN
• UniProt AC: Q9H9B1
• Protein Name: Histone-lysine N-methyltransferase EHMT1
• Gene Name: EHMT1
• Organism: Homo sapiens (Human).
• Sequence Length: 1298
• Subcellular Localization: Nucleus. Chromosome. Associates with euchromatic regions.
• Protein Description: Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53..
• Protein Sequence: MAAADAEAVPARGEPQQDCCVKTELLGEETPMAADEGSAEKQAGEAHMAADGETNGSCENSDASSHANAAKHTQDSARVNPQDGTNTLTRIAENGVSERDSEAAKQNHVTADDFVQTSVIGSNGYILNKPALQAQPLRTTSTLASSLPGHAAKTLPGGAGKGRTPSAFPQTPAAPPATLGEGSADTEDRKLPAPGADVKVHRARKTMPKSVVGLHAASKDPREVREARDHKEPKEEINKNISDFGRQQLLPPFPSLHQSLPQNQCYMATTKSQTACLPFVLAAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGSKGEKDLGASSLHVNGESLEMDSDEDDSEELEEDDGHGAEQAAAFPTEDSRTSKESMSEADRAQKMDGESEEEQESVDTGEEEEGGDESDLSSESSIKKKFLKRKGKTDSPWIKPARKRRRRSRKKPSGALGSESYKSSAGSAEQTAPGDSTGYMEVSLDSLDLRVKGILSSQAEGLANGPDVLETDGLQEVPLCSCRMETPKSREITTLANNQCMATESVDHELGRCTNSVVKYELMRPSNKAPLLVLCEDHRGRMVKHQCCPGCGYFCTAGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEESSKAKEVTIAKADTTSTVTPVPGQEKGSALEGRADTTTGSAAGPPLSEDDKLQGAASHVPEGFDPTGPAGLGRPTPGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQNKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLEAVKYLIKAGALVDPKDAEGSTCLHLAAKKGHYEVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVDLVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLNSQVWSALQMSKALQDSAPDRPSPVERIVSRDIARGYERIPIPCVNAVDSEPCPSNYKYVSQNCVTSPMNIDRNITHLQYCVCIDDCSSSNCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTRDMGWGVRSLQDIPPGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMAHQDLRFPRIAFFSTRLIEAGEQLGFDYGERFWDIKGKLFSCRCGSPKCRHSSAALAQRQASAAQEAQEDGLPDTSSAAAADPL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MAAADAEAV ------CCCCCCCCC	23.74	25489052
22	Sumoylation	PQQDCCVKTELLGEE CCCCCEEEEHHCCCC	23.51	25114211
38	Phosphorylation	PMAADEGSAEKQAGE CCCCCCCHHHHHHCC	31.07	25159151
73	Phosphorylation	HANAAKHTQDSARVN HHHHHHHCCCCCCCC	33.11	-
85	Phosphorylation	RVNPQDGTNTLTRIA CCCCCCCCCHHHHHH	33.06	24173317
87	Phosphorylation	NPQDGTNTLTRIAEN CCCCCCCHHHHHHHC	29.37	28555341
110	Phosphorylation	AAKQNHVTADDFVQT HHHHCCCCHHHHCCE	19.47	-
129	Acetylation	SNGYILNKPALQAQP CCCEEECCHHHCCCC	28.37	26051181
140	Phosphorylation	QAQPLRTTSTLASSL CCCCCCCHHHHHHCC	17.18	-
142	Phosphorylation	QPLRTTSTLASSLPG CCCCCHHHHHHCCCC	25.01	22210691
145	Phosphorylation	RTTSTLASSLPGHAA CCHHHHHHCCCCCHH	35.20	22210691
146	Phosphorylation	TTSTLASSLPGHAAK CHHHHHHCCCCCHHH	32.73	22210691
153	Trimethylation	SLPGHAAKTLPGGAG CCCCCHHHCCCCCCC	51.91	-
153	Methylation	SLPGHAAKTLPGGAG CCCCCHHHCCCCCCC	51.91	24129315
164	Phosphorylation	GGAGKGRTPSAFPQT CCCCCCCCCCCCCCC	30.20	25159151
171	Phosphorylation	TPSAFPQTPAAPPAT CCCCCCCCCCCCCCC	18.35	28555341
178 (in isoform 2)	Ubiquitination	-	40.86	21890473
183	Phosphorylation	PATLGEGSADTEDRK CCCCCCCCCCCCCCC	20.76	28985074
190	Sumoylation	SADTEDRKLPAPGAD CCCCCCCCCCCCCCC	71.76	28112733
199	Sumoylation	PAPGADVKVHRARKT CCCCCCCEEEECCCC	32.63	28112733
208 (in isoform 2)	Ubiquitination	-	30.88	21890473
209	Ubiquitination	RARKTMPKSVVGLHA ECCCCCCHHHHCHHH	45.09	21906983
209 (in isoform 1)	Ubiquitination	-	45.09	21906983
219	Acetylation	VGLHAASKDPREVRE HCHHHCCCCHHHHHH	68.01	23749302
231	Sumoylation	VREARDHKEPKEEIN HHHHHHCCCCHHHHH	78.85	28112733
234	Sumoylation	ARDHKEPKEEINKNI HHHCCCCHHHHHHCC	69.90	-
234	Sumoylation	ARDHKEPKEEINKNI HHHCCCCHHHHHHCC	69.90	28112733
239	Ubiquitination	EPKEEINKNISDFGR CCHHHHHHCCHHHHH	62.41	21890473
239	Ubiquitination	EPKEEINKNISDFGR CCHHHHHHCCHHHHH	62.41	21890473
239 (in isoform 3)	Ubiquitination	-	62.41	21890473
272	Phosphorylation	CYMATTKSQTACLPF CEECCCCCHHHHHHH	30.58	27251275
274	Phosphorylation	MATTKSQTACLPFVL ECCCCCHHHHHHHHH	26.82	27251275
295	Phosphorylation	KKKRRMGTYSLVPKK HHHHCCCCCCCCCCC	10.70	28555341
297	Phosphorylation	KRRMGTYSLVPKKKT HHCCCCCCCCCCCCC	23.80	28555341
301	Acetylation	GTYSLVPKKKTKVLK CCCCCCCCCCCHHHH	60.46	30588383
302	Acetylation	TYSLVPKKKTKVLKQ CCCCCCCCCCHHHHH	60.65	30588389
303	Acetylation	YSLVPKKKTKVLKQR CCCCCCCCCHHHHHH	60.76	30588395
304	Phosphorylation	SLVPKKKTKVLKQRT CCCCCCCCHHHHHHH	34.95	17081983
305	Phosphorylation	LVPKKKTKVLKQRTV CCCCCCCHHHHHHHH	55.22	17081983
311	Phosphorylation	TKVLKQRTVIEMFKS CHHHHHHHHHHHHHH	24.28	28348404
312	Phosphorylation	KVLKQRTVIEMFKSI HHHHHHHHHHHHHHH	3.67	18669648
317	Sumoylation	RTVIEMFKSITHSTV HHHHHHHHHHCCCCC	39.37	28112733
322	Phosphorylation	MFKSITHSTVGSKGE HHHHHCCCCCCCCCC	19.01	18669648
327	Sumoylation	THSTVGSKGEKDLGA CCCCCCCCCCCCCCC	66.22	28112733
327	Ubiquitination	THSTVGSKGEKDLGA CCCCCCCCCCCCCCC	66.22	-
335	Phosphorylation	GEKDLGASSLHVNGE CCCCCCCCEEEECCC	31.47	21406692
336	Phosphorylation	EKDLGASSLHVNGES CCCCCCCEEEECCCC	23.20	21406692
343	Phosphorylation	SLHVNGESLEMDSDE EEEECCCCCCCCCCC	30.91	18669648
348	Phosphorylation	GESLEMDSDEDDSEE CCCCCCCCCCCCHHH	41.40	25137130
353	Phosphorylation	MDSDEDDSEELEEDD CCCCCCCHHHHHHCC	45.34	25137130
372	Phosphorylation	EQAAAFPTEDSRTSK HHHHCCCCCCCCCCH	46.07	21406692
375	Phosphorylation	AAFPTEDSRTSKESM HCCCCCCCCCCHHHH	31.09	21406692
377	Phosphorylation	FPTEDSRTSKESMSE CCCCCCCCCHHHHCH	48.37	-
378	Phosphorylation	PTEDSRTSKESMSEA CCCCCCCCHHHHCHH	33.06	28985074
381	Phosphorylation	DSRTSKESMSEADRA CCCCCHHHHCHHHHH	31.20	28102081
383	Phosphorylation	RTSKESMSEADRAQK CCCHHHHCHHHHHHH	38.62	23401153
395	Phosphorylation	AQKMDGESEEEQESV HHHCCCCCHHHHHCC	56.70	25921289
401	Phosphorylation	ESEEEQESVDTGEEE CCHHHHHCCCCCCCC	25.61	25921289
404	Phosphorylation	EEQESVDTGEEEEGG HHHHCCCCCCCCCCC	44.32	25921289
430	Ubiquitination	KKKFLKRKGKTDSPW HHHHHHHCCCCCCCC	64.27	-
432	Sumoylation	KFLKRKGKTDSPWIK HHHHHCCCCCCCCCH	52.79	-
432	Methylation	KFLKRKGKTDSPWIK HHHHHCCCCCCCCCH	52.79	-
432	Sumoylation	KFLKRKGKTDSPWIK HHHHHCCCCCCCCCH	52.79	28112733
433	Phosphorylation	FLKRKGKTDSPWIKP HHHHCCCCCCCCCHH	50.83	23927012
435	Phosphorylation	KRKGKTDSPWIKPAR HHCCCCCCCCCHHHH	27.63	23401153
439	Ubiquitination	KTDSPWIKPARKRRR CCCCCCCHHHHHHHH	28.85	-
448	Phosphorylation	ARKRRRRSRKKPSGA HHHHHHHHCCCCCCC	46.79	20068231
451	Sumoylation	RRRRSRKKPSGALGS HHHHHCCCCCCCCCC	43.22	-
451	Sumoylation	RRRRSRKKPSGALGS HHHHHCCCCCCCCCC	43.22	-
453	Phosphorylation	RRSRKKPSGALGSES HHHCCCCCCCCCCCC	45.65	28985074
458	Phosphorylation	KPSGALGSESYKSSA CCCCCCCCCCCCCCC	24.99	22210691
460	Phosphorylation	SGALGSESYKSSAGS CCCCCCCCCCCCCCC	39.29	20068231
461	Phosphorylation	GALGSESYKSSAGSA CCCCCCCCCCCCCCC	15.73	22210691
463	Phosphorylation	LGSESYKSSAGSAEQ CCCCCCCCCCCCCCC	19.72	21406692
464	Phosphorylation	GSESYKSSAGSAEQT CCCCCCCCCCCCCCC	32.28	21406692
466	Phosphorylation	ESYKSSAGSAEQTAP CCCCCCCCCCCCCCC	29.36	17525332
467	Phosphorylation	SYKSSAGSAEQTAPG CCCCCCCCCCCCCCC	28.48	21406692
471	Phosphorylation	SAGSAEQTAPGDSTG CCCCCCCCCCCCCCC	26.73	21406692
476	Phosphorylation	EQTAPGDSTGYMEVS CCCCCCCCCCCEEEE	29.64	21406692
477	Phosphorylation	QTAPGDSTGYMEVSL CCCCCCCCCCEEEEH	36.75	21406692
479	Phosphorylation	APGDSTGYMEVSLDS CCCCCCCCEEEEHHH	7.09	21406692
483	Phosphorylation	STGYMEVSLDSLDLR CCCCEEEEHHHHHHH	17.10	25849741
486	Phosphorylation	YMEVSLDSLDLRVKG CEEEEHHHHHHHHHH	29.13	21406692
492	Sumoylation	DSLDLRVKGILSSQA HHHHHHHHHHHHHHH	33.52	28112733
496	Phosphorylation	LRVKGILSSQAEGLA HHHHHHHHHHHCHHH	20.62	26714015
497	Phosphorylation	RVKGILSSQAEGLAN HHHHHHHHHHCHHHC	29.93	17525332
511	Phosphorylation	NGPDVLETDGLQEVP CCCCEECCCCCCCCC	31.07	26714015
556	Phosphorylation	ELGRCTNSVVKYELM HHHHCCCCHHHHEEC	15.33	21214269
559	Sumoylation	RCTNSVVKYELMRPS HCCCCHHHHEECCCC	30.74	28112733
560	Phosphorylation	CTNSVVKYELMRPSN CCCCHHHHEECCCCC	11.59	21214269
568	Ubiquitination	ELMRPSNKAPLLVLC EECCCCCCCCEEEEE	56.57	-
638	Ubiquitination	GEESSKAKEVTIAKA CCCCCCCCEEEEEEC	57.35	-
644	Sumoylation	AKEVTIAKADTTSTV CCEEEEEECCCCCCC	42.83	-
644	Sumoylation	AKEVTIAKADTTSTV CCEEEEEECCCCCCC	42.83	28112733
644	Ubiquitination	AKEVTIAKADTTSTV CCEEEEEECCCCCCC	42.83	-
647	Phosphorylation	VTIAKADTTSTVTPV EEEEECCCCCCCCCC	27.75	25850435
648	Phosphorylation	TIAKADTTSTVTPVP EEEECCCCCCCCCCC	23.97	25850435
649	Phosphorylation	IAKADTTSTVTPVPG EEECCCCCCCCCCCC	23.99	25850435
650	Phosphorylation	AKADTTSTVTPVPGQ EECCCCCCCCCCCCC	26.72	25850435
652	Phosphorylation	ADTTSTVTPVPGQEK CCCCCCCCCCCCCCC	20.75	21815630
659	Sumoylation	TPVPGQEKGSALEGR CCCCCCCCCCCCCCC	50.72	28112733
659	Ubiquitination	TPVPGQEKGSALEGR CCCCCCCCCCCCCCC	50.72	-
680	Phosphorylation	SAAGPPLSEDDKLQG CCCCCCCCCCCCCCC	44.76	28985074
684	Sumoylation	PPLSEDDKLQGAASH CCCCCCCCCCCHHHC	55.84	28112733
708	Phosphorylation	PAGLGRPTPGLSQGP CCCCCCCCCCCCCCC	28.73	25159151
709 (in isoform 2)	Ubiquitination	-	44.40	21890473
729	Phosphorylation	SALIALDSEKPKKLR HHHHHHCCCCCCCCC	48.66	24667141
731	Sumoylation	LIALDSEKPKKLRFH HHHHCCCCCCCCCCC	67.12	28112733
731	Ubiquitination	LIALDSEKPKKLRFH HHHHCCCCCCCCCCC	67.12	-
740	Ubiquitination	KKLRFHPKQLYFSAR CCCCCCHHHHEEECC	44.35	21890473
740	Acetylation	KKLRFHPKQLYFSAR CCCCCCHHHHEEECC	44.35	26051181
740	Ubiquitination	KKLRFHPKQLYFSAR CCCCCCHHHHEEECC	44.35	21890473
740 (in isoform 3)	Ubiquitination	-	44.35	21890473
796 (in isoform 2)	Ubiquitination	-	11.32	21890473
823	Ubiquitination	NNHLEAVKYLIKAGA HCHHHHHHHHHHCCC	41.19	-
827	Ubiquitination	EAVKYLIKAGALVDP HHHHHHHHCCCCCCC	37.87	21906983
827 (in isoform 1)	Ubiquitination	-	37.87	21906983
827 (in isoform 3)	Ubiquitination	-	37.87	21890473
852	Phosphorylation	LAAKKGHYEVVQYLL HHHHCCHHHHHHHHH	21.06	24043423
857	Phosphorylation	GHYEVVQYLLSNGQM CHHHHHHHHHHCCCC	9.67	24043423
860	Phosphorylation	EVVQYLLSNGQMDVN HHHHHHHHCCCCCEE	36.00	24043423
875	Phosphorylation	CQDDGGWTPMIWATE CCCCCCCCCEEEEEE	13.27	24043423
881	Phosphorylation	WTPMIWATEYKHVDL CCCEEEEEECCCHHH	25.50	24043423
883	Phosphorylation	PMIWATEYKHVDLVK CEEEEEECCCHHHHH	11.31	24043423
890	Ubiquitination	YKHVDLVKLLLSKGS CCCHHHHHHHHHCCC	40.49	890473
890 (in isoform 3)	Ubiquitination	-	40.49	21890473
895	Ubiquitination	LVKLLLSKGSDINIR HHHHHHHCCCCCCCC	63.50	21890473
895 (in isoform 3)	Ubiquitination	-	63.50	21890473
941	Phosphorylation	AVNIHGDSPLHIAAR EEECCCCCHHHHHHC	33.67	20873877
952	Phosphorylation	IAARENRYDCVVLFL HHHCCCCCCEEEEEE	25.85	-
968	Ubiquitination	RDSDVTLKNKEGETP CCCCCEECCCCCCCC	57.52	21906983
968 (in isoform 1)	Ubiquitination	-	57.52	21906983
998	Phosphorylation	MSKALQDSAPDRPSP HHHHHHHCCCCCCCH	28.83	29255136
1002	Methylation	LQDSAPDRPSPVERI HHHCCCCCCCHHHHH	32.32	-
1004	Phosphorylation	DSAPDRPSPVERIVS HCCCCCCCHHHHHHH	42.33	29255136
1018	Phosphorylation	SRDIARGYERIPIPC HHHHHCCCCCCCCCC	9.11	17081983
1040	Phosphorylation	PCPSNYKYVSQNCVT CCCCCCCEECCCCCC	8.58	21406692
1042	Phosphorylation	PSNYKYVSQNCVTSP CCCCCEECCCCCCCC	16.76	21406692
1047	Phosphorylation	YVSQNCVTSPMNIDR EECCCCCCCCCCCCC	29.34	30108239
1048	Phosphorylation	VSQNCVTSPMNIDRN ECCCCCCCCCCCCCC	11.18	23401153
1057	Phosphorylation	MNIDRNITHLQYCVC CCCCCCCCCCCEEEE	21.78	24043423
1061	Phosphorylation	RNITHLQYCVCIDDC CCCCCCCEEEEEECC	8.00	24043423
1069	Phosphorylation	CVCIDDCSSSNCMCG EEEEECCCCCCCCCC	43.74	24043423
1070	Phosphorylation	VCIDDCSSSNCMCGQ EEEECCCCCCCCCCE	31.72	24043423
1071	Phosphorylation	CIDDCSSSNCMCGQL EEECCCCCCCCCCEE	20.11	24043423
1079	Phosphorylation	NCMCGQLSMRCWYDK CCCCCEEEEEEEECC	9.04	24043423
1086	Ubiquitination	SMRCWYDKDGRLLPE EEEEEECCCCCCCCC	46.52	-
1228	Phosphorylation	FPRIAFFSTRLIEAG CCHHEEEEHHHHHHH	13.23	24114839
1276	Phosphorylation	ALAQRQASAAQEAQE HHHHHHHHHHHHHHH	18.79	27251275

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of EHMT1_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of EHMT1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of EHMT1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
TRI15_HUMAN	TRIM15	physical	20211142
CTBP1_HUMAN	CTBP1	physical	16702210
CTBP2_HUMAN	CTBP2	physical	16702210
P53_HUMAN	TP53	physical	20588255
H31_HUMAN	HIST1H3A	physical	20118233
P53_HUMAN	TP53	physical	20118233
H31T_HUMAN	HIST3H3	physical	12700765
TF65_HUMAN	RELA	physical	21131967
DNM3A_HUMAN	DNMT3A	physical	22086334
EHMT1_HUMAN	EHMT1	physical	22086334
MPP8_HUMAN	MPHOSPH8	physical	22086334
PRDM6_HUMAN	PRDM6	physical	16537907
DNLI1_HUMAN	LIG1	physical	28803780
DNLI1_MOUSE	Lig1	physical	28803780

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• 610253: Kleefstra syndrome (KLESTS)
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"ATM and ATR substrate analysis reveals extensive protein networksresponsive to DNA damage.";
Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III,Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N.,Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.;
Science 316:1160-1166(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-497, AND MASSSPECTROMETRY.
PubMed: 17525332