MLRV_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: MLRV_HUMAN
• UniProt AC: P10916
• Protein Name: Myosin regulatory light chain 2, ventricular/cardiac muscle isoform {ECO:0000305}
• Gene Name: MYL2 {ECO:0000312|HGNC:HGNC:7583}
• Organism: Homo sapiens (Human).
• Sequence Length: 166
• Subcellular Localization: Cytoplasm, myofibril, sarcomere, A band .
• Protein Description: Contractile protein that plays a role in heart development and function (By similarity). Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion; as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin binding sites to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force (By similarity). During cardiogenesis plays an early role in cardiac contractility by promoting cardiac myofibril assembly (By similarity)..
• Protein Sequence: MAPKKAKKRAGGANSNVFSMFEQTQIQEFKEAFTIMDQNRDGFIDKNDLRDTFAALGRVNVKNEEIDEMIKEAPGPINFTVFLTMFGEKLKGADPEETILNAFKVFDPEGKGVLKADYVREMLTTQAERFSKEEVDQMFAAFPPDVTGNLDYKNLVHIITHGEEKD

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Methylation	------MAPKKAKKR ------CCCHHHHHH	25.31	-
14	Deamidated asparagine	KKRAGGANSNVFSMF HHHCCCCCCCHHHHH	37.07	-
14	Deamidation	KKRAGGANSNVFSMF HHHCCCCCCCHHHHH	37.07	20445002
15	Phosphorylation	KRAGGANSNVFSMFE HHCCCCCCCHHHHHH	33.56	20038585
19	Phosphorylation	GANSNVFSMFEQTQI CCCCCHHHHHHHHHH	20.48	9418861
24	Phosphorylation	VFSMFEQTQIQEFKE HHHHHHHHHHHHHHH	22.05	26437602
34	Phosphorylation	QEFKEAFTIMDQNRD HHHHHHHHHHCCCCC	23.57	26437602
52	Phosphorylation	DKNDLRDTFAALGRV CHHHHHHHHHHHCCC	14.56	-
80	Phosphorylation	APGPINFTVFLTMFG CCCCEEEEEEEECCC	13.08	22673903
98	Phosphorylation	KGADPEETILNAFKV CCCCHHHHHHHHHHH	29.15	26437602
104	Acetylation	ETILNAFKVFDPEGK HHHHHHHHHCCCCCC	39.31	160891
111	Acetylation	KVFDPEGKGVLKADY HHCCCCCCCCCCHHH	44.15	30584983
118	Phosphorylation	KGVLKADYVREMLTT CCCCCHHHHHHHHHH	13.28	19764811
124	Phosphorylation	DYVREMLTTQAERFS HHHHHHHHHHHHHCC	18.62	26437602
125	Phosphorylation	YVREMLTTQAERFSK HHHHHHHHHHHHCCH	23.09	26437602
152	Phosphorylation	DVTGNLDYKNLVHII CCCCCCCHHCCEEEE	12.63	-
153	Acetylation	VTGNLDYKNLVHIIT CCCCCCHHCCEEEEC	43.39	30584989
160	Phosphorylation	KNLVHIITHGEEKD- HCCEEEECCCCCCC-	24.19	26437602
165	Acetylation	IITHGEEKD------ EECCCCCCC------	65.52	8010075

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
15	S	Phosphorylation	Kinase	DAPK3	O43293	Uniprot
15	S	Phosphorylation	Kinase	MYLK	Q15746	GPS
15	S	Phosphorylation	Kinase	ROCK2	O75116	PSP
118	Y	Phosphorylation	Kinase	PKM	P14618	PSP
-	K	Ubiquitination	E3 ubiquitin ligase	TRIM63	Q969Q1	PMID:22199232

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of MLRV_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of MLRV_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
UBP6_HUMAN	USP6	physical	16555005
NHP2_HUMAN	NHP2	physical	21988832
MYL5_HUMAN	MYL5	physical	28514442
MYO5B_HUMAN	MYO5B	physical	28514442
MYO19_HUMAN	MYO19	physical	28514442
ASPM_HUMAN	ASPM	physical	28514442
MYO5A_HUMAN	MYO5A	physical	28514442
IL36G_HUMAN	IL36G	physical	28514442
KPYM_HUMAN	PKM	physical	25412762

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• 608758: Cardiomyopathy, familial hypertrophic 10 (CMH10)
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.