UBP6_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: UBP6_HUMAN
• UniProt AC: P35125
• Protein Name: Ubiquitin carboxyl-terminal hydrolase 6
• Gene Name: USP6
• Organism: Homo sapiens (Human).
• Sequence Length: 1406
• Subcellular Localization: Cell membrane. Cytoplasm. Endosome. Localizes to the plasma membrane and to filamentous structures within the cell corresponding to ARF6 regulated tubular endosomes. Activation of RAC1 and CDC42 can direct the relocalization of USP6 to the plasma mem
• Protein Description: Deubiquitinase with an ATP-independent isopeptidase activity, cleaving at the C-terminus of the ubiquitin moiety. Catalyzes its own deubiquitination. In vitro, isoform 2, but not isoform 3, shows deubiquitinating activity. Promotes plasma membrane localization of ARF6 and selectively regulates ARF6-dependent endocytic protein trafficking. Is able to initiate tumorigenesis by inducing the production of matrix metalloproteinases following NF-kappa-B activation..
• Protein Sequence: MDMVENADSLQAQERKDILMKYDKGHRAGLPEDKGPEPVGINSSIDRFGILHETELPPVTAREAKKIRREMTRTSKWMEMLGEWETYKHSSKLIDRVYKGIPMNIRGPVWSVLLNIQEIKLKNPGRYQIMKERGKRSSEHIHHIDLDVRTTLRNHVFFRDRYGAKQRELFYILLAYSEYNPEVGYCRDLSHITALFLLYLPEEDAFWALVQLLASERHSLPGFHSPNGGTVQGLQDQQEHVVPKSQPKTMWHQDKEGLCGQCASLGCLLRNLIDGISLGLTLRLWDVYLVEGEQVLMPITSIALKVQQKRLMKTSRCGLWARLRNQFFDTWAMNDDTVLKHLRASTKKLTRKQGDLPPPAKREQGSLAPRPVPASRGGKTLCKGYRQAPPGPPAQFQRPICSASPPWASRFSTPCPGGAVREDTYPVGTQGVPSLALAQGGPQGSWRFLEWKSMPRLPTDLDIGGPWFPHYDFEWSCWVRAISQEDQLATCWQAEHCGEVHNKDMSWPEEMSFTANSSKIDRQKVPTEKGATGLSNLGNTCFMNSSIQCVSNTQPLTQYFISGRHLYELNRTNPIGMKGHMAKCYGDLVQELWSGTQKSVAPLKLRRTIAKYAPKFDGFQQQDSQELLAFLLDGLHEDLNRVHEKPYVELKDSDGRPDWEVAAEAWDNHLRRNRSIIVDLFHGQLRSQVKCKTCGHISVRFDPFNFLSLPLPMDSYMDLEITVIKLDGTTPVRYGLRLNMDEKYTGLKKQLRDLCGLNSEQILLAEVHDSNIKNFPQDNQKVQLSVSGFLCAFEIPVPSSPISASSPTQIDFSSSPSTNGMFTLTTNGDLPKPIFIPNGMPNTVVPCGTEKNFTNGMVNGHMPSLPDSPFTGYIIAVHRKMMRTELYFLSPQENRPSLFGMPLIVPCTVHTRKKDLYDAVWIQVSWLARPLPPQEASIHAQDRDNCMGYQYPFTLRVVQKDGNSCAWCPQYRFCRGCKIDCGEDRAFIGNAYIAVDWHPTALHLRYQTSQERVVDKHESVEQSRRAQAEPINLDSCLRAFTSEEELGESEMYYCSKCKTHCLATKKLDLWRLPPFLIIHLKRFQFVNDQWIKSQKIVRFLRESFDPSAFLVPRDPALCQHKPLTPQGDELSKPRILAREVKKVDAQSSAGKEDMLLSKSPSSLSANISSSPKGSPSSSRKSGTSCPSSKNSSPNSSPRTLGRSKGRLRLPQIGSKNKPSSSKKNLDASKENGAGQICELADALSRGHMRGGSQPELVTPQDHEVALANGFLYEHEACGNGCGDGYSNGQLGNHSEEDSTDDQREDTHIKPIYNLYAISCHSGILSGGHYITYAKNPNCKWYCYNDSSCEELHPDEIDTDSAYILFYEQQGIDYAQFLPKIDGKKMADTSSTDEDSESDYEKYSMLQ

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
22	Phosphorylation	RKDILMKYDKGHRAG HHHHHHHHCCCCCCC	14.66	23312004
49 (in isoform 2)	Phosphorylation	-	23.57	21964256
86	Phosphorylation	EMLGEWETYKHSSKL HHHCCHHHHCCHHHH	38.65	22210691
87	Phosphorylation	MLGEWETYKHSSKLI HHCCHHHHCCHHHHH	8.66	22210691
90	Phosphorylation	EWETYKHSSKLIDRV CHHHHCCHHHHHHHH	25.64	22210691
91	Phosphorylation	WETYKHSSKLIDRVY HHHHCCHHHHHHHHH	31.06	25002506
98	Phosphorylation	SKLIDRVYKGIPMNI HHHHHHHHCCCCCCC	12.37	-
127	Phosphorylation	KLKNPGRYQIMKERG ECCCCCHHHHHHHCC	14.33	21406692
177	Phosphorylation	FYILLAYSEYNPEVG HHHHHHHHHHCCCCC	28.18	22210691
179	Phosphorylation	ILLAYSEYNPEVGYC HHHHHHHHCCCCCCC	30.03	22210691
215	Phosphorylation	ALVQLLASERHSLPG HHHHHHHHCCCCCCC	34.82	24719451
309	Ubiquitination	IALKVQQKRLMKTSR HHHHHHHHHHHHHCC	30.33	30230243
330	Phosphorylation	LRNQFFDTWAMNDDT HHHHHHCEECCCCHH	15.23	25850435
337	Phosphorylation	TWAMNDDTVLKHLRA EECCCCHHHHHHHHH	30.51	25850435
345	Phosphorylation	VLKHLRASTKKLTRK HHHHHHHHHHHHHHH	34.03	28152594
346	Phosphorylation	LKHLRASTKKLTRKQ HHHHHHHHHHHHHHC	31.08	28152594
361	Ubiquitination	GDLPPPAKREQGSLA CCCCCCHHHCCCCCC	63.34	-
366	Phosphorylation	PAKREQGSLAPRPVP CHHHCCCCCCCCCCC	22.51	24719451
453	Phosphorylation	WRFLEWKSMPRLPTD EEEEEECCCCCCCCC	34.78	25404012
562	Phosphorylation	PLTQYFISGRHLYEL CCEEEEECCCCEEEC	21.63	24719451
578	Acetylation	RTNPIGMKGHMAKCY CCCCCCCHHHHHHHH	40.21	7365999
583	Acetylation	GMKGHMAKCYGDLVQ CCHHHHHHHHHHHHH	22.03	7366007
645	Ubiquitination	DLNRVHEKPYVELKD HHHHHHCCCCEEEEC	27.11	29967540
734	Phosphorylation	DGTTPVRYGLRLNMD CCCCCCEEEEECCCC	22.27	18083107
744	Phosphorylation	RLNMDEKYTGLKKQL ECCCCHHHHCHHHHH	12.18	23403867
745	Phosphorylation	LNMDEKYTGLKKQLR CCCCHHHHCHHHHHH	47.29	24719451
954	Phosphorylation	MGYQYPFTLRVVQKD CCCCCCEEEEEEECC	14.78	24719451
1041	Phosphorylation	DSCLRAFTSEEELGE HHHHHHCCCHHHHCC	33.95	-
1124	Phosphorylation	LCQHKPLTPQGDELS HHCCCCCCCCCCCCC	23.30	28985074
1131	Phosphorylation	TPQGDELSKPRILAR CCCCCCCCCCHHHHH	38.27	25262027
1141	Acetylation	RILAREVKKVDAQSS HHHHHHHHHHHCCCC	42.18	7693069
1142	Acetylation	ILAREVKKVDAQSSA HHHHHHHHHHCCCCC	50.99	7693077
1147	Phosphorylation	VKKVDAQSSAGKEDM HHHHHCCCCCCHHHH	24.71	18669648
1158	Acetylation	KEDMLLSKSPSSLSA HHHHHCCCCCCCCCC	67.37	7693085
1159	Phosphorylation	EDMLLSKSPSSLSAN HHHHCCCCCCCCCCC	27.00	18669648
1161	Phosphorylation	MLLSKSPSSLSANIS HHCCCCCCCCCCCCC	51.51	23663014
1162	Phosphorylation	LLSKSPSSLSANISS HCCCCCCCCCCCCCC	29.72	29978859
1164	Phosphorylation	SKSPSSLSANISSSP CCCCCCCCCCCCCCC	22.63	29978859
1168	Phosphorylation	SSLSANISSSPKGSP CCCCCCCCCCCCCCC	25.27	29978859
1169	Phosphorylation	SLSANISSSPKGSPS CCCCCCCCCCCCCCC	47.03	18669648
1170	Phosphorylation	LSANISSSPKGSPSS CCCCCCCCCCCCCCC	24.68	28674151
1174	Phosphorylation	ISSSPKGSPSSSRKS CCCCCCCCCCCCCCC	27.95	29978859
1176	Phosphorylation	SSPKGSPSSSRKSGT CCCCCCCCCCCCCCC	42.51	23663014
1177	Phosphorylation	SPKGSPSSSRKSGTS CCCCCCCCCCCCCCC	37.23	30576142
1178	Phosphorylation	PKGSPSSSRKSGTSC CCCCCCCCCCCCCCC	48.33	29978859
1181	Phosphorylation	SPSSSRKSGTSCPSS CCCCCCCCCCCCCCC	46.31	28985074
1183	Phosphorylation	SSSRKSGTSCPSSKN CCCCCCCCCCCCCCC	33.96	30576142
1184	Phosphorylation	SSRKSGTSCPSSKNS CCCCCCCCCCCCCCC	27.79	25849741
1187	Phosphorylation	KSGTSCPSSKNSSPN CCCCCCCCCCCCCCC	59.27	23090842
1188	Phosphorylation	SGTSCPSSKNSSPNS CCCCCCCCCCCCCCC	22.46	23090842
1191	Phosphorylation	SCPSSKNSSPNSSPR CCCCCCCCCCCCCCC	51.41	25849741
1192	Phosphorylation	CPSSKNSSPNSSPRT CCCCCCCCCCCCCCC	37.18	25159151
1195	Phosphorylation	SKNSSPNSSPRTLGR CCCCCCCCCCCCCCC	45.14	22199227
1196	Phosphorylation	KNSSPNSSPRTLGRS CCCCCCCCCCCCCCC	25.66	25159151
1199	Phosphorylation	SPNSSPRTLGRSKGR CCCCCCCCCCCCCCC	36.63	22199227
1203	Phosphorylation	SPRTLGRSKGRLRLP CCCCCCCCCCCCCCC	37.35	29514088
1214	Phosphorylation	LRLPQIGSKNKPSSS CCCCCCCCCCCCCCC	34.38	22617229
1221	Phosphorylation	SKNKPSSSKKNLDAS CCCCCCCCCCCCCHH	52.57	30631047
1228	Phosphorylation	SKKNLDASKENGAGQ CCCCCCHHHHCCHHH	39.92	30631047
1312	Ubiquitination	DTHIKPIYNLYAISC CCCCCCCEEEEEEEC	13.98	23503661
1317	Ubiquitination	PIYNLYAISCHSGIL CCEEEEEEECCCCCC	2.59	23503661
1334	Ubiquitination	GHYITYAKNPNCKWY CCEEEEECCCCCEEE	63.62	23503661
1339	Ubiquitination	YAKNPNCKWYCYNDS EECCCCCEEEEECCC	48.08	23503661
1341	Phosphorylation	KNPNCKWYCYNDSSC CCCCCEEEEECCCCC	3.16	-
1343	Phosphorylation	PNCKWYCYNDSSCEE CCCEEEEECCCCCCC	13.30	-
1346	Phosphorylation	KWYCYNDSSCEELHP EEEEECCCCCCCCCC	33.00	-
1347	Phosphorylation	WYCYNDSSCEELHPD EEEECCCCCCCCCCC	28.54	-
1388	Phosphorylation	DGKKMADTSSTDEDS CCEECCCCCCCCCCC	18.29	24719451
1389	Phosphorylation	GKKMADTSSTDEDSE CEECCCCCCCCCCCH	31.61	24719451
1390	Phosphorylation	KKMADTSSTDEDSES EECCCCCCCCCCCHH	41.80	28348404
1391	Phosphorylation	KMADTSSTDEDSESD ECCCCCCCCCCCHHH	43.08	28348404
1395	Phosphorylation	TSSTDEDSESDYEKY CCCCCCCCHHHHHHH	36.63	24719451
1397	Phosphorylation	STDEDSESDYEKYSM CCCCCCHHHHHHHHC	49.82	28348404
1399	Phosphorylation	DEDSESDYEKYSMLQ CCCCHHHHHHHHCCC	24.49	28348404
1402	Phosphorylation	SESDYEKYSMLQ--- CHHHHHHHHCCC---	6.33	28348404
1403	Phosphorylation	ESDYEKYSMLQ---- HHHHHHHHCCC----	24.88	28348404

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of UBP6_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of UBP6_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of UBP6_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
RB33B_HUMAN	RAB33B	physical	16923123
ARF6_HUMAN	ARF6	physical	15509780
MLRV_HUMAN	MYL2	physical	16555005
ANR44_HUMAN	ANKRD44	physical	16555005
A4_HUMAN	APP	physical	21832049
MDFI_HUMAN	MDFI	physical	19060904
EXOS8_HUMAN	EXOSC8	physical	25416956
JUN_HUMAN	JUN	physical	29061731
JAK1_HUMAN	JAK1	physical	27440725

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.