IRF8_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: IRF8_HUMAN
• UniProt AC: Q02556
• Protein Name: Interferon regulatory factor 8
• Gene Name: IRF8
• Organism: Homo sapiens (Human).
• Sequence Length: 426
• Subcellular Localization: Nucleus . Cytoplasm . In resting macrophages, localizes in the cytoplasm. Translocated in the nucleus upon IFN-gamma induction.
• Protein Description: Plays a role as a transcriptional activator or repressor. [PubMed: 25122610 Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS) Plays a negative regulatory role in cells of the immune system. Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of genes (By similarity]
• Protein Sequence: MCDRNGGRRLRQWLIEQIDSSMYPGLIWENEEKSMFRIPWKHAGKQDYNQEVDASIFKAWAVFKGKFKEGDKAEPATWKTRLRCALNKSPDFEEVTDRSQLDISEPYKVYRIVPEEEQKCKLGVATAGCVNEVTEMECGRSEIDELIKEPSVDDYMGMIKRSPSPPEACRSQLLPDWWAQQPSTGVPLVTGYTTYDAHHSAFSQMVISFYYGGKLVGQATTTCPEGCRLSLSQPGLPGTKLYGPEGLELVRFPPADAIPSERQRQVTRKLFGHLERGVLLHSSRQGVFVKRLCQGRVFCSGNAVVCKGRPNKLERDEVVQVFDTSQFFRELQQFYNSQGRLPDGRVVLCFGEEFPDMAPLRSKLILVQIEQLYVRQLAEEAGKSCGAGSVMQAPEEPPPDQVFRMFPDICASHQRSFFRENQQITV

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
36	Ubiquitination	ENEEKSMFRIPWKHA ECCCCCCCCCCCCCC	9.16	21890473
55	Phosphorylation	YNQEVDASIFKAWAV CCHHHCHHHHHHHHH	24.88	24719451
89	Phosphorylation	LRCALNKSPDFEEVT HHHHHHCCCCHHHCC	28.73	28348404
107	Phosphorylation	QLDISEPYKVYRIVP HCCCCCCCEEEEECC	14.72	18195016
110	Phosphorylation	ISEPYKVYRIVPEEE CCCCCEEEEECCHHH	7.17	18195016
162	Phosphorylation	YMGMIKRSPSPPEAC HCCCCCCCCCCCHHH	25.40	26657352
164	Phosphorylation	GMIKRSPSPPEACRS CCCCCCCCCCHHHHH	55.09	26657352
230	Phosphorylation	CPEGCRLSLSQPGLP CCCCCEEECCCCCCC	13.46	28450419
232	Phosphorylation	EGCRLSLSQPGLPGT CCCEEECCCCCCCCC	30.84	28450419
240	Ubiquitination	QPGLPGTKLYGPEGL CCCCCCCEEECCCCE	45.70	22817900
260	Phosphorylation	PPADAIPSERQRQVT CCHHCCCHHHHHHHH	39.02	24719451
269	Methylation	RQRQVTRKLFGHLER HHHHHHHHHHHHHHH	38.51	-
373	Phosphorylation	LVQIEQLYVRQLAEE HHHHHHHHHHHHHHH	8.03	22817900
416	Phosphorylation	ICASHQRSFFRENQQ HHHHHHHHHHHHCCC	23.36	24719451

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
-	K	Ubiquitination	E3 ubiquitin ligase	TRIM21	P19474	PMID:17579016
-	K	Ubiquitination	E3 ubiquitin ligase	CBL	P22681	PMID:15837792

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of IRF8_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of IRF8_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
CSN2_HUMAN	COPS2	physical	10991940
IRF1_HUMAN	IRF1	physical	9742224
IRF1_HUMAN	IRF1	physical	7768900
IRF2_HUMAN	IRF2	physical	7768900
RO52_HUMAN	TRIM21	physical	17579016
ETV6_HUMAN	ETV6	physical	17579016
IRF8_HUMAN	IRF8	physical	17579016
SQSTM_HUMAN	SQSTM1	physical	19201866
CDK4_HUMAN	CDK4	physical	21903422
CSN2_HUMAN	COPS2	physical	21903422
CSN4_HUMAN	COPS4	physical	21903422
CSN5_HUMAN	COPS5	physical	21903422
CSN6_HUMAN	COPS6	physical	21903422
CSN1_HUMAN	GPS1	physical	21903422
IRF6_HUMAN	IRF6	physical	21903422
TRI18_HUMAN	MID1	physical	21903422
CBL_HUMAN	CBL	physical	15837792
UBP4_HUMAN	USP4	physical	28477415

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• 614893: Immunodeficiency 32A (IMD32A)
• 614894: Immunodeficiency 32B (IMD32B)
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.