CNBL2_ARATH - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: CNBL2_ARATH
• UniProt AC: Q8LAS7
• Protein Name: Calcineurin B-like protein 2
• Gene Name: CBL2
• Organism: Arabidopsis thaliana (Mouse-ear cress).
• Sequence Length: 226
• Subcellular Localization: Vacuole membrane ; Lipid-anchor . The tonoplast localization is S-acylation dependent and is abolished by 2-bromopalmitate (2-BP) treatment.
• Protein Description: Acts as a calcium sensor. CBL proteins interact with CIPK serine-threonine protein kinases. Binding of a CBL protein to the regulatory NAF domain of a CIPK protein lead to the activation of the kinase in a calcium-dependent manner. Binds four calcium ions per subunit. Mediates the activation of AKT1 by CIPK proteins (CIPK6, CIPK16, and CIPK23) in response to low potassium conditions and in the context of stomatal movement. Mediates the inactivation of the proton pump AHA2 by CIPK11. Probably involved in regulating signaling responses to abscisic acid..
• Protein Sequence: MSQCVDGIKHLCTSVLGCFDLDLYKQSGGLGDPELLARDTVFSVSEIEALYELFKKISSAVIDDGLINKEEFQLALFKTNKKESLFADRVFDLFDTKHNGILGFEEFARALSVFHPNAPIDDKIHFSFQLYDLKQQGFIERQEVKQMVVATLAESGMNLKDTVIEDIIDKTFEEADTKHDGKIDKEEWRSLVLRHPSLLKNMTLQYLKDITTTFPSFVFHSQVEDT

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
4	S-palmitoylation	----MSQCVDGIKHL ----CCCHHHHHHHH	2.06	22547024
12	S-palmitoylation	VDGIKHLCTSVLGCF HHHHHHHHHHHHHCC	2.39	22547024
18	S-palmitoylation	LCTSVLGCFDLDLYK HHHHHHHCCCHHHHH	1.78	22547024
51	Phosphorylation	VSEIEALYELFKKIS HHHHHHHHHHHHHHH	20.03	19880383
212	Phosphorylation	QYLKDITTTFPSFVF HHHHHHHHHCCHHHC	27.12	30407730
213	Phosphorylation	YLKDITTTFPSFVFH HHHHHHHHCCHHHCC	25.54	30407730
216	Phosphorylation	DITTTFPSFVFHSQV HHHHHCCHHHCCCCC	29.83	22547024
221	Phosphorylation	FPSFVFHSQVEDT-- CCHHHCCCCCCCC--	25.18	30407730
226	Phosphorylation	FHSQVEDT------- CCCCCCCC-------	27.31	30407730

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
216	S	Phosphorylation	Kinase	CIPK11	O22932	Uniprot
216	S	Phosphorylation	Kinase	CIPK14	Q9LZW4	Uniprot

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of CNBL2_ARATH !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of CNBL2_ARATH !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
CIPKB_ARATH	SIP4	physical	17483306
CIPKB_ARATH	SIP4	physical	11402167
CIPK9_ARATH	CIPK9	physical	11402167
CIPKO_ARATH	SOS2	physical	11402167
CIPK1_ARATH	CIPK1	physical	11230129
CIPKD_ARATH	CIPK13	physical	11230129
CIPKE_ARATH	SR1	physical	11230129
CIPKD_ARATH	CIPK13	physical	14730130
CIPKI_ARATH	CIPK18	physical	14730130
CIPK7_ARATH	CIPK7	physical	14730130
CIPKE_ARATH	SR1	physical	14730130
CIPKE_ARATH	SR1	physical	19832944
CIPKO_ARATH	SOS2	physical	19832944
CIPKL_ARATH	CIPK21	physical	21798944
CIPKA_ARATH	SIP1	physical	21798944
CIPKE_ARATH	SR1	physical	18237745
CIPK9_ARATH	CIPK9	physical	23109687
CIPK3_ARATH	CIPK3	physical	25646412
CIPK9_ARATH	CIPK9	physical	25646412
CIPKN_ARATH	CIPK23	physical	25646412
CIPKQ_ARATH	AT5G21326	physical	25646412
CIPKE_ARATH	SR1	physical	24521877
MTN1_ARATH	MTN1	physical	26259190
CIPKL_ARATH	CIPK21	physical	26198257

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.