dbPTM

ABCA1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	ABCA1_HUMAN
UniProt AC	O95477
Protein Name	ATP-binding cassette sub-family A member 1
Gene Name	ABCA1
Organism	Homo sapiens (Human).
Sequence Length	2261
Subcellular Localization	Membrane Multi-pass membrane protein .
Protein Description	cAMP-dependent and sulfonylurea-sensitive anion transporter. Key gatekeeper influencing intracellular cholesterol transport..
Protein Sequence	MACWPQLRLLLWKNLTFRRRQTCQLLLEVAWPLFIFLILISVRLSYPPYEQHECHFPNKAMPSAGTLPWVQGIICNANNPCFRYPTPGEAPGVVGNFNKSIVARLFSDARRLLLYSQKDTSMKDMRKVLRTLQQIKKSSSNLKLQDFLVDNETFSGFLYHNLSLPKSTVDKMLRADVILHKVFLQGYQLHLTSLCNGSKSEEMIQLGDQEVSELCGLPREKLAAAERVLRSNMDILKPILRTLNSTSPFPSKELAEATKTLLHSLGTLAQELFSMRSWSDMRQEVMFLTNVNSSSSSTQIYQAVSRIVCGHPEGGGLKIKSLNWYEDNNYKALFGGNGTEEDAETFYDNSTTPYCNDLMKNLESSPLSRIIWKALKPLLVGKILYTPDTPATRQVMAEVNKTFQELAVFHDLEGMWEELSPKIWTFMENSQEMDLVRMLLDSRDNDHFWEQQLDGLDWTAQDIVAFLAKHPEDVQSSNGSVYTWREAFNETNQAIRTISRFMECVNLNKLEPIATEVWLINKSMELLDERKFWAGIVFTGITPGSIELPHHVKYKIRMDIDNVERTNKIKDGYWDPGPRADPFEDMRYVWGGFAYLQDVVEQAIIRVLTGTEKKTGVYMQQMPYPCYVDDIFLRVMSRSMPLFMTLAWIYSVAVIIKGIVYEKEARLKETMRIMGLDNSILWFSWFISSLIPLLVSAGLLVVILKLGNLLPYSDPSVVFVFLSVFAVVTILQCFLISTLFSRANLAAACGGIIYFTLYLPYVLCVAWQDYVGFTLKIFASLLSPVAFGFGCEYFALFEEQGIGVQWDNLFESPVEEDGFNLTTSVSMMLFDTFLYGVMTWYIEAVFPGQYGIPRPWYFPCTKSYWFGEESDEKSHPGSNQKRISEICMEEEPTHLKLGVSIQNLVKVYRDGMKVAVDGLALNFYEGQITSFLGHNGAGKTTTMSILTGLFPPTSGTAYILGKDIRSEMSTIRQNLGVCPQHNVLFDMLTVEEHIWFYARLKGLSEKHVKAEMEQMALDVGLPSSKLKSKTSQLSGGMQRKLSVALAFVGGSKVVILDEPTAGVDPYSRRGIWELLLKYRQGRTIILSTHHMDEADVLGDRIAIISHGKLCCVGSSLFLKNQLGTGYYLTLVKKDVESSLSSCRNSSSTVSYLKKEDSVSQSSSDAGLGSDHESDTLTIDVSAISNLIRKHVSEARLVEDIGHELTYVLPYEAAKEGAFVELFHEIDDRLSDLGISSYGISETTLEEIFLKVAEESGVDAETSDGTLPARRNRRAFGDKQSCLRPFTEDDAADPNDSDIDPESRETDLLSGMDGKGSYQVKGWKLTQQQFVALLWKRLLIARRSRKGFFAQIVLPAVFVCIALVFSLIVPPFGKYPSLELQPWMYNEQYTFVSNDAPEDTGTLELLNALTKDPGFGTRCMEGNPIPDTPCQAGEEEWTTAPVPQTIMDLFQNGNWTMQNPSPACQCSSDKIKKMLPVCPPGAGGLPPPQRKQNTADILQDLTGRNISDYLVKTYVQIIAKSLKNKIWVNEFRYGGFSLGVSNTQALPPSQEVNDAIKQMKKHLKLAKDSSADRFLNSLGRFMTGLDTKNNVKVWFNNKGWHAISSFLNVINNAILRANLQKGENPSHYGITAFNHPLNLTKQQLSEVALMTTSVDVLVSICVIFAMSFVPASFVVFLIQERVSKAKHLQFISGVKPVIYWLSNFVWDMCNYVVPATLVIIIFICFQQKSYVSSTNLPVLALLLLLYGWSITPLMYPASFVFKIPSTAYVVLTSVNLFIGINGSVATFVLELFTDNKLNNINDILKSVFLIFPHFCLGRGLIDMVKNQAMADALERFGENRFVSPLSWDLVGRNLFAMAVEGVVFFLITVLIQYRFFIRPRPVNAKLSPLNDEDEDVRRERQRILDGGGQNDILEIKELTKIYRRKRKPAVDRICVGIPPGECFGLLGVNGAGKSSTFKMLTGDTTVTRGDAFLNKNSILSNIHEVHQNMGYCPQFDAITELLTGREHVEFFALLRGVPEKEVGKVGEWAIRKLGLVKYGEKYAGNYSGGNKRKLSTAMALIGGPPVVFLDEPTTGMDPKARRFLWNCALSVVKEGRSVVLTSHSMEECEALCTRMAIMVNGRFRCLGSVQHLKNRFGDGYTIVVRIAGSNPDLKPVQDFFGLAFPGSVLKEKHRNMLQYQLPSSLSSLARIFSILSQSKKRLHIEDYSVSQTTLDQVFVNFAKDQSDDDHLKDLSLHKNQTVVDVAVLTSFLQDEKVKESYV

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
3	S-palmitoylation	-----MACWPQLRLL -----CCHHHHHHHH	9.19	19556522
14	N-linked_Glycosylation	LRLLLWKNLTFRRRQ HHHHHHHCCHHHCHH	32.50	UniProtKB CARBOHYD
23	S-palmitoylation	TFRRRQTCQLLLEVA HHHCHHHHHHHHHHH	1.71	19556522
98	N-linked_Glycosylation	PGVVGNFNKSIVARL CCCCCCCCHHHHHHH	40.96	19159218
115	Phosphorylation	DARRLLLYSQKDTSM HHHHHHHHHCCCCCH	14.71	29978859
116	Phosphorylation	ARRLLLYSQKDTSMK HHHHHHHHCCCCCHH	30.62	29978859
120	Phosphorylation	LLYSQKDTSMKDMRK HHHHCCCCCHHHHHH	37.44	29978859
121	Phosphorylation	LYSQKDTSMKDMRKV HHHCCCCCHHHHHHH	33.69	29978859
151	N-linked_Glycosylation	LQDFLVDNETFSGFL EEEEEECCCCCCCHH	42.61	UniProtKB CARBOHYD
161	N-linked_Glycosylation	FSGFLYHNLSLPKST CCCHHHCCCCCCHHH	20.12	UniProtKB CARBOHYD
196	N-linked_Glycosylation	LHLTSLCNGSKSEEM CHHHHHCCCCCCHHH	64.17	UniProtKB CARBOHYD
231	Phosphorylation	AAERVLRSNMDILKP HHHHHHHHCHHHHHH	32.14	108417611
244	N-linked_Glycosylation	KPILRTLNSTSPFPS HHHHHHCCCCCCCCC	42.50	19159218
246	Phosphorylation	ILRTLNSTSPFPSKE HHHHCCCCCCCCCHH	39.07	24719451
292	N-linked_Glycosylation	VMFLTNVNSSSSSTQ HHEEECCCCCCCHHH	37.94	UniProtKB CARBOHYD
325	Phosphorylation	KIKSLNWYEDNNYKA EEEECCEEECCCEEE	16.60	59303065
337	N-linked_Glycosylation	YKALFGGNGTEEDAE EEECCCCCCCHHHHH	55.37	UniProtKB CARBOHYD
349	N-linked_Glycosylation	DAETFYDNSTTPYCN HHHHHCCCCCCHHHH	29.85	UniProtKB CARBOHYD
350	O-linked_Glycosylation	AETFYDNSTTPYCND HHHHCCCCCCHHHHH	30.66	29351928
386	Phosphorylation	LVGKILYTPDTPATR HCCEEEECCCCHHHH	15.96	22210691
389	Phosphorylation	KILYTPDTPATRQVM EEEECCCCHHHHHHH	18.94	22210691
392	Phosphorylation	YTPDTPATRQVMAEV ECCCCHHHHHHHHHH	23.88	22210691
400	N-linked_Glycosylation	RQVMAEVNKTFQELA HHHHHHHHHHHHHHH	29.20	UniProtKB CARBOHYD
478	N-linked_Glycosylation	PEDVQSSNGSVYTWR HHHHHHCCCCEEEHH	52.59	UniProtKB CARBOHYD
489	N-linked_Glycosylation	YTWREAFNETNQAIR EEHHHHHHHHHHHHH	63.33	UniProtKB CARBOHYD
521	N-linked_Glycosylation	ATEVWLINKSMELLD HHHHHHHCCHHHHHH	28.33	UniProtKB CARBOHYD
820	N-linked_Glycosylation	PVEEDGFNLTTSVSM CCCCCCCCCHHHHHH	41.84	UniProtKB CARBOHYD
870	Phosphorylation	SYWFGEESDEKSHPG CEECCCCCCCCCCCC	46.93	28842319
884	Phosphorylation	GSNQKRISEICMEEE CCCCHHHHHHHCCCC	25.58	23684312
1031	Phosphorylation	SKLKSKTSQLSGGMQ HHHCCCHHHCCHHHH	32.62	68852465
1042	Phosphorylation	GGMQRKLSVALAFVG HHHHHHHHHHEEECC	14.37	12196520
1110	S-palmitoylation	IISHGKLCCVGSSLF EEECCCEEEECCCEE	1.69	19556522
1111	S-palmitoylation	ISHGKLCCVGSSLFL EECCCEEEECCCEEE	5.87	19556522
1137	Phosphorylation	LVKKDVESSLSSCRN EEECHHHHHHHHHCC	36.11	23312004
1138	Phosphorylation	VKKDVESSLSSCRNS EECHHHHHHHHHCCC	20.82	23312004
1140	Phosphorylation	KDVESSLSSCRNSSS CHHHHHHHHHCCCCC	29.37	28348404
1141	Phosphorylation	DVESSLSSCRNSSST HHHHHHHHHCCCCCH	23.53	28348404
1144	N-linked_Glycosylation	SSLSSCRNSSSTVSY HHHHHHCCCCCHHHE	51.04	UniProtKB CARBOHYD
1145	Phosphorylation	SLSSCRNSSSTVSYL HHHHHCCCCCHHHEE	13.30	26657352
1146	Phosphorylation	LSSCRNSSSTVSYLK HHHHCCCCCHHHEEC	33.31	28348404
1147	Phosphorylation	SSCRNSSSTVSYLKK HHHCCCCCHHHEECH	32.42	28857561
1148	Phosphorylation	SCRNSSSTVSYLKKE HHCCCCCHHHEECHH	18.54	28857561
1159	Phosphorylation	LKKEDSVSQSSSDAG ECHHCCCCCCCCCCC	28.44	24275569
1206	Phosphorylation	DIGHELTYVLPYEAA HHCCCEEEECCHHHH	16.77	21402379
1242	Phosphorylation	SSYGISETTLEEIFL CCCCCCCCCHHHHHH	29.86	22817900
1243	Phosphorylation	SYGISETTLEEIFLK CCCCCCCCHHHHHHH	28.28	22817900
1255	Phosphorylation	FLKVAEESGVDAETS HHHHHHHHCCCCCCC	35.30	22817900
1261	Phosphorylation	ESGVDAETSDGTLPA HHCCCCCCCCCCCCC	33.56	24719451
1262	Phosphorylation	SGVDAETSDGTLPAR HCCCCCCCCCCCCCH	26.08	68998007
1280	Phosphorylation	RAFGDKQSCLRPFTE CHHCCCCCCCCCCCC	22.36	21902226
1286	Phosphorylation	QSCLRPFTEDDAADP CCCCCCCCCCCCCCC	40.98	12869555
1294	N-linked_Glycosylation	EDDAADPNDSDIDPE CCCCCCCCCCCCCHH	63.06	UniProtKB CARBOHYD
1296	Phosphorylation	DAADPNDSDIDPESR CCCCCCCCCCCHHHH	43.01	25159151
1302	Phosphorylation	DSDIDPESRETDLLS CCCCCHHHHHHCCCC	40.58	29514088
1305	Phosphorylation	IDPESRETDLLSGMD CCHHHHHHCCCCCCC	30.73	29514088
1309	Phosphorylation	SRETDLLSGMDGKGS HHHHCCCCCCCCCCC	39.09	29514088
1453	N-linked_Glycosylation	MDLFQNGNWTMQNPS HHHHHCCCCCCCCCC	38.30	UniProtKB CARBOHYD
1504	N-linked_Glycosylation	LQDLTGRNISDYLVK HHHHHCCCHHHHHHH	39.19	UniProtKB CARBOHYD
1513	Phosphorylation	SDYLVKTYVQIIAKS HHHHHHHHHHHHHHH	5.66	27134283
1536	Phosphorylation	EFRYGGFSLGVSNTQ EEECCCEECCCCCCC	27.73	22159673
1540	Phosphorylation	GGFSLGVSNTQALPP CCEECCCCCCCCCCC	32.15	22159681
1548	Phosphorylation	NTQALPPSQEVNDAI CCCCCCCCHHHHHHH	36.71	108935907
1582	Phosphorylation	NSLGRFMTGLDTKNN HHHHHHHCCCCCCCC	31.70	113136027
1586	Phosphorylation	RFMTGLDTKNNVKVW HHHCCCCCCCCEEEE	40.49	18452278
1637	N-linked_Glycosylation	TAFNHPLNLTKQQLS EECCCCCCCCHHHHH	50.55	UniProtKB CARBOHYD
1953	Phosphorylation	GVNGAGKSSTFKMLT ECCCCCCCCCEECCC	33.42	30576142
1954	Phosphorylation	VNGAGKSSTFKMLTG CCCCCCCCCEECCCC	41.15	30576142
2044	N-linked_Glycosylation	YGEKYAGNYSGGNKR CCCCCCCCCCCCCHH	21.45	UniProtKB CARBOHYD
2050	Acetylation	GNYSGGNKRKLSTAM CCCCCCCHHHHHHHH	54.94	19826679
2054	Phosphorylation	GGNKRKLSTAMALIG CCCHHHHHHHHHHHC	19.70	15722566
2101	Phosphorylation	GRSVVLTSHSMEECE CCEEEEECCCHHHHH	14.92	46156249
2234	Phosphorylation	DDHLKDLSLHKNQTV CCHHHHHHHCCCCCH	38.26	26657352
2238	N-linked_Glycosylation	KDLSLHKNQTVVDVA HHHHHCCCCCHHHHH	31.82	UniProtKB CARBOHYD
2240	Phosphorylation	LSLHKNQTVVDVAVL HHHCCCCCHHHHHHH	31.71	28857561

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
1042	S	Phosphorylation	Kinase	PRKACA	P17612	GPS
1042	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
1042	S	Phosphorylation	Kinase	PKA	-	Uniprot
1042	S	Phosphorylation	Kinase	PKA_GROUP	-	PhosphoELM
1242	T	Phosphorylation	Kinase	CSNK2A1	P68400	GPS
1242	T	Phosphorylation	Kinase	CK2-FAMILY	-	GPS
1243	T	Phosphorylation	Kinase	CSNK2A1	P68400	GPS
1243	T	Phosphorylation	Kinase	CK2-FAMILY	-	GPS
1255	S	Phosphorylation	Kinase	CSNK2A1	P68400	GPS
1255	S	Phosphorylation	Kinase	CK2-FAMILY	-	GPS
2054	S	Phosphorylation	Kinase	EGFR	P00533	PSP
2054	S	Phosphorylation	Kinase	PRKACA	P17612	GPS
2054	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
2054	S	Phosphorylation	Kinase	PKA	-	Uniprot
2054	S	Phosphorylation	Kinase	PKA_GROUP	-	PhosphoELM

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
2054	S	Phosphorylation		12196520
Phosphorylation on Ser-2054 regulates phospholipid efflux.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
231	Phosphorylation	230 (1)	R ⇒ C	rs9282541	HDL cholesterol	23505323

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
FADD_HUMAN	FADD	physical	12235128
APOA1_HUMAN	APOA1	physical	12084722
SNTB2_HUMAN	SNTB2	physical	12054535
UGPA_HUMAN	UGP2	physical	12054535
SDHB_HUMAN	SDHB	physical	12054535
AOXA_HUMAN	AOX1	physical	12054535
PRP8_HUMAN	PRPF8	physical	12054535
HGS_HUMAN	HGS	physical	21520210
CSN5_HUMAN	COPS5	physical	19268428
STX12_HUMAN	STX12	physical	15469992
FLOT1_HUMAN	FLOT1	physical	15469992
CDC42_HUMAN	CDC42	physical	16443932
SPTC1_HUMAN	SPTLC1	physical	18484747
TGM2_HUMAN	TGM2	physical	21988832
NEMO_HUMAN	IKBKG	physical	21988832
NR1H2_HUMAN	NR1H2	physical	20951680
NR1H2_HUMAN	NR1H2	physical	25838426
APOA1_HUMAN	APOA1	physical	27017521
CSN2_HUMAN	COPS2	physical	27017521

Drug and Disease Associations

Kegg Disease
H00159	Tangier disease
H00930	Hypoalphalipoproteinemia
OMIM Disease
205400	High density lipoprotein deficiency 1 (HDLD1)
604091	High density lipoprotein deficiency 2 (HDLD2)
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of ABCA1_HUMAN

Related Literatures of Post-Translational Modification

N-linked Glycosylation
Reference	PubMed
"Glycoproteomics analysis of human liver tissue by combination ofmultiple enzyme digestion and hydrazide chemistry."; Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.; J. Proteome Res. 8:651-661(2009). Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-98 AND ASN-244, AND MASSSPECTROMETRY.	19159218 [Abstract]
Palmitoylation
Reference	PubMed
"Palmitoylation of ATP-binding cassette transporter A1 is essentialfor its trafficking and function."; Singaraja R.R., Kang M.H., Vaid K., Sanders S.S., Vilas G.L.,Arstikaitis P., Coutinho J., Drisdel R.C., El-Husseini Ael D.,Green W.N., Berthiaume L., Hayden M.R.; Circ. Res. 105:138-147(2009). Cited for: PALMITOYLATION AT CYS-3; CYS-23; CYS-1110 AND CYS-1111, ANDSUBCELLULAR LOCATION.	19556522 [Abstract]
Phosphorylation
Reference	PubMed
"Protein kinase A site-specific phosphorylation regulates ATP-bindingcassette A1 (ABCA1)-mediated phospholipid efflux."; See R.H., Caday-Malcolm R.A., Singaraja R.R., Zhou S., Silverston A.,Huber M.T., Moran J., James E.R., Janoo R., Savill J.M., Rigot V.,Zhang L.H., Wang M., Chimini G., Wellington C.L., Tafuri S.R.,Hayden M.R.; J. Biol. Chem. 277:41835-41842(2002). Cited for: PHOSPHORYLATION AT SER-1042 AND SER-2054.	12196520 [Abstract]

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