WNT3A_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: WNT3A_HUMAN
• UniProt AC: P56704
• Protein Name: Protein Wnt-3a
• Gene Name: WNT3A
• Organism: Homo sapiens (Human).
• Sequence Length: 352
• Subcellular Localization: Secreted, extracellular space, extracellular matrix . Secreted .
• Protein Description: Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family. [PubMed: 20093360]
• Protein Sequence: MAPLGYFLLLCSLKQALGSYPIWWSLAVGPQYSSLGSQPILCASIPGLVPKQLRFCRNYVEIMPSVAEGIKIGIQECQHQFRGRRWNCTTVHDSLAIFGPVLDKATRESAFVHAIASAGVAFAVTRSCAEGTAAICGCSSRHQGSPGKGWKWGGCSEDIEFGGMVSREFADARENRPDARSAMNRHNNEAGRQAIASHMHLKCKCHGLSGSCEVKTCWWSQPDFRAIGDFLKDKYDSASEMVVEKHRESRGWVETLRPRYTYFKVPTERDLVYYEASPNFCEPNPETGSFGTRDRTCNVSSHGIDGCDLLCCGRGHNARAERRREKCRCVFHWCCYVSCQECTRVYDVHTCK

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
65	Phosphorylation	NYVEIMPSVAEGIKI HHHHHCHHHHHHHHC	19.31	-
87	N-linked_Glycosylation	QFRGRRWNCTTVHDS HHCCCCCCCEEHHHH	16.08	21244856
209	O-palmitoyl serine	KCKCHGLSGSCEVKT EEEECCCCCCCEEEE	33.00	-
209	O-palmitoleoylation	KCKCHGLSGSCEVKT EEEECCCCCCCEEEE	33.00	24292069
235	Phosphorylation	GDFLKDKYDSASEMV HHHHHHHCCCHHHHH	25.84	21955146
237	Phosphorylation	FLKDKYDSASEMVVE HHHHHCCCHHHHHHH	30.51	21955146
239	Phosphorylation	KDKYDSASEMVVEKH HHHCCCHHHHHHHHH	30.12	21955146
298	N-linked_Glycosylation	GTRDRTCNVSSHGID CCCCCCCCCCCCCCC	35.77	21244856

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of WNT3A_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of WNT3A_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of WNT3A_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
FZD1_HUMAN	FZD1	physical	10557084
KCTD1_HUMAN	KCTD1	genetic	24736394
CALX_HUMAN	CANX	physical	26186194
GRP78_HUMAN	HSPA5	physical	26186194
WNT3_HUMAN	WNT3	physical	26186194
2AAB_HUMAN	PPP2R1B	physical	26186194
2A5D_HUMAN	PPP2R5D	physical	26186194
2A5A_HUMAN	PPP2R5A	physical	26186194
2A5B_HUMAN	PPP2R5B	physical	26186194
2A5E_HUMAN	PPP2R5E	physical	26186194
FEM1B_HUMAN	FEM1B	physical	26186194
2ABD_HUMAN	PPP2R2D	physical	26186194
TRAF2_HUMAN	TRAF2	physical	26186194
WNT3_HUMAN	WNT3	physical	28514442
2A5A_HUMAN	PPP2R5A	physical	28514442
2A5B_HUMAN	PPP2R5B	physical	28514442
2A5E_HUMAN	PPP2R5E	physical	28514442
2A5D_HUMAN	PPP2R5D	physical	28514442
2AAB_HUMAN	PPP2R1B	physical	28514442
2ABD_HUMAN	PPP2R2D	physical	28514442
CALX_HUMAN	CANX	physical	28514442
SDF2L_HUMAN	SDF2L1	physical	28514442
FEM1B_HUMAN	FEM1B	physical	28514442
TRAF2_HUMAN	TRAF2	physical	28514442

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Palmitoylation

"WLS-dependent secretion of WNT3A requires Ser209 acylation andvacuolar acidification.";
Coombs G.S., Yu J., Canning C.A., Veltri C.A., Covey T.M.,Cheong J.K., Utomo V., Banerjee N., Zhang Z.H., Jadulco R.C.,Concepcion G.P., Bugni T.S., Harper M.K., Mihalek I., Jones C.M.,Ireland C.M., Virshup D.M.;
J. Cell Sci. 123:3357-3367(2010).
Cited for: PALMITOYLATION AT SER-209 BY PORCN, AND MUTAGENESIS OF SER-209.
PubMed: 20826466