WIPI2_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: WIPI2_MOUSE
• UniProt AC: Q80W47
• Protein Name: WD repeat domain phosphoinositide-interacting protein 2
• Gene Name: Wipi2
• Organism: Mus musculus (Mouse).
• Sequence Length: 445
• Subcellular Localization: Preautophagosomal structure membrane ; Peripheral membrane protein ; Cytoplasmic side . Enriched at preautophagosomal structure membranes in response to PtdIns3P. Dissociates from mature phagosome membranes.
• Protein Description: Early component of the autophagy machinery being involved in formation of preautophagosomal structures and their maturation into mature phagosomes in response to phosphatidylinositol 3-phosphate (PtdIns3P). Recruits the ATG12-ATG5-ATG16L1 complex to omegasomes, resulting in ATG8 family proteins lipidation and starvation-induced autophagy. Omegasomes are derived from ER and serve as intermediates for genesis of isolation membrane (also called preautophagosome) from ER cisternae membranes..
• Protein Sequence: MNLASQSGEAGAGQLLFANFNQDNTSLAVGSKSGYKFFSLSSVDKLEQIYECTDTEDVCIVERLFSSSLVAIVSLKAPRKLKVCHFKKGTEICNYSYSNTILAVKLNRQRLIVCLEESLYIHNIRDMKVLHTIRETPPNPAGLCALSINNDNCYLAYPGSASIGEVQVFDTINLRAANMIPAHDSPLAALAFDASGTKLATASEKGTVIRVFSIPEGQKLFEFRRGVKRCVSICSLAFSMDGMFLSASSNTETVHIFKLEAVREKPPEEPTTWTGYFGKVLMASTSYLPSQVTEMFNQGRAFATVRLPFCGHKNICSLTTIQKIPRLLVGASDGYLYMYNLDPQEGGECALMRQHRLDGSMETTSEIVDSASHDCPLATQTYGTAAAKGAYVPSSPTRLGKGQDANLEAYTDDLGAVGGACLEDEASALRLDEDSEHPPMILRTD

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
79	Ubiquitination	IVSLKAPRKLKVCHF EEECCCCCCEEEEEC	62.89	27667366
198	Ubiquitination	AFDASGTKLATASEK EECCCCCEEEEECCC	39.38	22790023
203	Phosphorylation	GTKLATASEKGTVIR CCEEEEECCCCCEEE	35.17	21454597
205	Malonylation	KLATASEKGTVIRVF EEEEECCCCCEEEEE	58.34	26320211
205	Ubiquitination	KLATASEKGTVIRVF EEEEECCCCCEEEEE	58.34	27667366
207	Phosphorylation	ATASEKGTVIRVFSI EEECCCCCEEEEEEC	24.67	21454597
276	Phosphorylation	EPTTWTGYFGKVLMA CCCCCCCCCCCHHHH	11.20	29514104
323	Acetylation	CSLTTIQKIPRLLVG CCCHHHCCCCEEEEC	51.32	22826441
323	Ubiquitination	CSLTTIQKIPRLLVG CCCHHHCCCCEEEEC	51.32	22790023
360	Phosphorylation	RQHRLDGSMETTSEI EEEECCCCCEEHHHH	17.08	26239621
363	Phosphorylation	RLDGSMETTSEIVDS ECCCCCEEHHHHHHH	27.29	26239621
364	Phosphorylation	LDGSMETTSEIVDSA CCCCCEEHHHHHHHH	15.85	26239621
365	Phosphorylation	DGSMETTSEIVDSAS CCCCEEHHHHHHHHC	32.52	26239621
370	Phosphorylation	TTSEIVDSASHDCPL EHHHHHHHHCCCCCC	22.30	26239621
372	Phosphorylation	SEIVDSASHDCPLAT HHHHHHHCCCCCCCC	24.66	26239621
379	Phosphorylation	SHDCPLATQTYGTAA CCCCCCCCEECCCCC	29.18	25159016
381	Phosphorylation	DCPLATQTYGTAAAK CCCCCCEECCCCCCC	21.40	25159016
382	Phosphorylation	CPLATQTYGTAAAKG CCCCCEECCCCCCCC	11.58	25159016
384	Phosphorylation	LATQTYGTAAAKGAY CCCEECCCCCCCCCC	11.60	24224561
391	Phosphorylation	TAAAKGAYVPSSPTR CCCCCCCCCCCCCCC	22.89	28833060
394	Phosphorylation	AKGAYVPSSPTRLGK CCCCCCCCCCCCCCC	38.62	24925903
395	Phosphorylation	KGAYVPSSPTRLGKG CCCCCCCCCCCCCCC	24.99	24925903
397	Phosphorylation	AYVPSSPTRLGKGQD CCCCCCCCCCCCCCC	40.24	24925903

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
395	S	Phosphorylation	Kinase	MTOR	Q9JLN9	PSP
-	K	Ubiquitination	E3 ubiquitin ligase	Huwe1	Q7TMY8	PMID:30646805

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of WIPI2_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of WIPI2_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
DSG1_HUMAN	DSG1	physical	26496610
T2FA_HUMAN	GTF2F1	physical	26496610
MYO10_HUMAN	MYO10	physical	26496610
UPAR_HUMAN	PLAUR	physical	26496610
VA0D1_HUMAN	ATP6V0D1	physical	26496610
PACS2_HUMAN	PACS2	physical	26496610
MACF1_HUMAN	MACF1	physical	26496610
BCAS3_HUMAN	BCAS3	physical	26496610
CHD9_HUMAN	CHD9	physical	26496610
DI3L2_HUMAN	DIS3L2	physical	26496610

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Large-scale phosphorylation analysis of mouse liver.";
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-394 AND SER-395, ANDMASS SPECTROMETRY.
PubMed: 17242355