TOP2_DROME - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: TOP2_DROME
• UniProt AC: P15348
• Protein Name: DNA topoisomerase 2 {ECO:0000305}
• Gene Name: Top2 {ECO:0000303|PubMed:25340780, ECO:0000312|FlyBase:FBgn0284220}
• Organism: Drosophila melanogaster (Fruit fly).
• Sequence Length: 1447
• Subcellular Localization: Nucleus . Chromosome . Cytoplasm . Nuclear at interphase, becoming diffusely dispersed in the cytoplasm at later cell cycle stages (PubMed:10885744, PubMed:10751154). However in early developing embryos, some may remain associated with condensed chro
• Protein Description: Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. [PubMed: 6308011]
• Protein Sequence: MENGNKALSIEQMYQKKSQLEHILLRPDSYIGSVEFTKELMWVYDNSQNRMVQKEISFVPGLYKIFDEILVNAADNKQRDKSMNTIKIDIDPERNMVSVWNNGQGIPVTMHKEQKMYVPTMIFGHLLTSSNYNDDEKKVTGGRNGYGAKLCNIFSTSFTVETATREYKKSFKQTWGNNMGKASDVQIKDFNGTDYTRITFSPDLAKFKMDRLDEDIVALMSRRAYDVAASSKGVSVFLNGNKLGVRNFKDYIDLHIKNTDDDSGPPIKIVHEVANERWEVACCPSDRGFQQVSFVNSIATYKGGRHVDHVVDNLIKQLLEVLKKKNKGGINIKPFQVRNHLWVFVNCLIENPTFDSQTKENMTLQQKGFGSKCTLSEKFINNMSKSGIVESVLAWAKFKAQNDIAKTGGRKSSKIKGIPKLEDANEAGGKNSIKCTLILTEGDSAKSLAVSGLGVIGRDLYGVFPLRGKLLNVREANFKQLSENAEINNLCKIIGLQYKKKYLTEDDLKTLRYGKVMIMTDQDQDGSHIKGLLINFIHTNWPELLRLPFLEEFITPIVKATKKNEELSFYSLPEFEEWKNDTANHHTYNIKYYKGLGTSTSKEAKEYFQDMDRHRILFKYDGSVDDESIVMAFSKKHIESRKVWLTNHMDEVKRRKELGLPERYLYTKGTKSITYADFINLELVLFSNADNERSIPSLVDGLKPGQRKVMFTCFKRNDKREVKVAQLSGSVAEMSAYHHGEVSLQMTIVNLAQNFVGANNINLLEPRGQFGTRLSGGKDCASARYIFTIMSPLTRLIYHPLDDPLLDYQVDDGQKIEPLWYLPIIPMVLVNGAEGIGTGWSTKISNYNPREIMKNLRKMINGQEPSVMHPWYKNFLGRMEYVSDGRYIQTGNIQILSGNRLEISELPVGVWTQNYKENVLEPLSNGTEKVKGIISEYREYHTDTTVRFVISFAPGEFERIHAEEGGFYRVFKLTTTLSTNQMHAFDQNNCLRRFPTAIDILKEYYKLRREYYARRRDFLVGQLTAQADRLSDQARFILEKCEKKLVVENKQRKAMCDELLKRGYRPDPVKEWQRRIKMEDAEQADEEDEEEEEAAPSVSSKAKKEKEVDPEKAFKKLTDVKKFDYLLGMSMWMLTEEKKNELLKQRDTKLSELESLRKKTPEMLWLDDLDALESKLNEVEEKERAEEQGINLKTAKALKGQKSASAKGRKVKSMGGGAGAGDVFPDPDGEPVEFKITEEIIKKMAAAAKVAQAAKEPKKPKEPKEPKVKKEPKGKQIKAEPDASGDEVDEFDAMVEGGSKTSPKAKKAVVKKEPGEKKPRQKKENGGGLKQSKIDFSKAKAKKSDDDVEEVTPRAERPGRRQASKKIDYSSLFSDEEEDGGNVGSDDDGNASDDDSPKRPAKRGREDESSGGAKKKAPPKKRRAVIESDDDDIEIDEDDDDDSDFNC

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
263	Phosphorylation	IKNTDDDSGPPIKIV EECCCCCCCCCCEEE	61.26	27626673
333	Acetylation	NKGGINIKPFQVRNH CCCCCCCCCEEECCE	34.98	21791702
333	Acetylation	NKGGINIKPFQVRNH CCCCCCCCCEEECCE	34.98	-
509	Acetylation	YLTEDDLKTLRYGKV CCCHHHHHHCCCCCE	53.13	-
509	Acetylation	YLTEDDLKTLRYGKV CCCHHHHHHCCCCCE	53.13	21791702
1061	Acetylation	AMCDELLKRGYRPDP HHHHHHHHCCCCCCH	57.30	-
1061	Acetylation	AMCDELLKRGYRPDP HHHHHHHHCCCCCCH	57.30	21791702
1064	Phosphorylation	DELLKRGYRPDPVKE HHHHHCCCCCCHHHH	24.01	22817900
1213	Phosphorylation	AKGRKVKSMGGGAGA CCCCCCEECCCCCCC	26.16	19429919
1284	Phosphorylation	IKAEPDASGDEVDEF CCCCCCCCCCCCCCC	54.91	21082442
1299	Phosphorylation	DAMVEGGSKTSPKAK HHHCCCCCCCCHHHH	43.55	19429919
1301	Phosphorylation	MVEGGSKTSPKAKKA HCCCCCCCCHHHHHH	52.54	22668510
1302	Phosphorylation	VEGGSKTSPKAKKAV CCCCCCCCHHHHHHH	28.30	19429919
1344	Phosphorylation	SKAKAKKSDDDVEEV HHHHCCCCCCCHHHC	45.63	19429919
1352	Phosphorylation	DDDVEEVTPRAERPG CCCHHHCCCCCCCCC	15.44	19429919
1369	Phosphorylation	QASKKIDYSSLFSDE CCCCCCCHHHHCCCC	12.02	19429919
1370	Phosphorylation	ASKKIDYSSLFSDEE CCCCCCHHHHCCCCC	19.47	19429919
1371	Phosphorylation	SKKIDYSSLFSDEEE CCCCCHHHHCCCCCC	27.49	19429919
1374	Phosphorylation	IDYSSLFSDEEEDGG CCHHHHCCCCCCCCC	49.78	19429919
1385	Phosphorylation	EDGGNVGSDDDGNAS CCCCCCCCCCCCCCC	33.32	19429919
1392	Phosphorylation	SDDDGNASDDDSPKR CCCCCCCCCCCCCCC	45.64	19429919
1396	Phosphorylation	GNASDDDSPKRPAKR CCCCCCCCCCCCHHC	39.04	19429919
1409	Phosphorylation	KRGREDESSGGAKKK HCCCCCCCCCCCCCC	46.75	30478224
1410	Phosphorylation	RGREDESSGGAKKKA CCCCCCCCCCCCCCC	38.92	30478224
1428	Phosphorylation	KRRAVIESDDDDIEI HCCEEEECCCCCCCC	34.90	21082442
1443	Phosphorylation	DEDDDDDSDFNC--- CCCCCCCCCCCC---	51.61	21082442

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of TOP2_DROME !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of TOP2_DROME !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of TOP2_DROME !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
MOF_DROME	mof	genetic	25340516
ATR_DROME	mei-41	genetic	25340516
ATM_DROME	tefu	genetic	25340516
PHP_DROME	ph-p	physical	11172718
MSL1_DROME	msl-1	physical	25694028
TOP2_DROME	Top2	physical	11447119
MLE_DROME	mle	physical	25694028
ISWI_DROME	Iswi	physical	9252192
MMD4_DROME	mod(mdg4)	physical	21304601

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Phosphoproteome analysis of Drosophila melanogaster embryos.";
Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
J. Proteome Res. 7:1675-1682(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1284; SER-1344;THR-1352; SER-1374; SER-1385; SER-1392 AND SER-1396, AND MASSSPECTROMETRY.
PubMed: 18327897