dbPTM

TLK2_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	TLK2_HUMAN
UniProt AC	Q86UE8
Protein Name	Serine/threonine-protein kinase tousled-like 2
Gene Name	TLK2
Organism	Homo sapiens (Human).
Sequence Length	772
Subcellular Localization	Nucleus. Cytoplasm, perinuclear region. Cytoplasm, cytoskeleton. Colocalizes with the cytoplasmic intermediate filament system during the G1 phase of the cell cycle. Present in the perinuclear region at S phase and in the nucleus at late G2.
Protein Description	Serine/threonine-protein kinase involved in the process of chromatin assembly and probably also DNA replication, transcription, repair, and chromosome segregation. Phosphorylates the chromatin assembly factors ASF1A AND ASF1B. Phosphorylation of ASF1A prevents its proteasome-mediated degradation, thereby enhancing chromatin assembly. Negative regulator of amino acid starvation-induced autophagy..
Protein Sequence	MMEELHSLDPRRQELLEARFTGVGVSKGPLNSESSNQSLCSVGSLSDKEVETPEKKQNDQRNRKRKAEPYETSQGKGTPRGHKISDYFEFAGGSAPGTSPGRSVPPVARSSPQHSLSNPLPRRVEQPLYGLDGSAAKEATEEQSALPTLMSVMLAKPRLDTEQLAQRGAGLCFTFVSAQQNSPSSTGSGNTEHSCSSQKQISIQHRQTQSDLTIEKISALENSKNSDLEKKEGRIDDLLRANCDLRRQIDEQQKMLEKYKERLNRCVTMSKKLLIEKSKQEKMACRDKSMQDRLRLGHFTTVRHGASFTEQWTDGYAFQNLIKQQERINSQREEIERQRKMLAKRKPPAMGQAPPATNEQKQRKSKTNGAENETPSSGNTELKDTAPALGAHSLLRLTLAEYHEQEEIFKLRLGHLKKEEAEIQAELERLERVRNLHIRELKRIHNEDNSQFKDHPTLNDRYLLLHLLGRGGFSEVYKAFDLTEQRYVAVKIHQLNKNWRDEKKENYHKHACREYRIHKELDHPRIVKLYDYFSLDTDSFCTVLEYCEGNDLDFYLKQHKLMSEKEARSIIMQIVNALKYLNEIKPPIIHYDLKPGNILLVNGTACGEIKITDFGLSKIMDDDSYNSVDGMELTSQGAGTYWYLPPECFVVGKEPPKISNKVDVWSVGVIFYQCLYGRKPFGHNQSQQDILQENTILKATEVQFPPKPVVTPEAKAFIRRCLAYRKEDRIDVQQLACDPYLLPHIRKSVSTSSPAGAAIASTSGASNNSSSN

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
7	Phosphorylation	-MMEELHSLDPRRQE -CHHHHHHCCHHHHH	47.28	27732954
26	Phosphorylation	RFTGVGVSKGPLNSE HHCCCCCCCCCCCCC	26.43	30576142
26 (in isoform 2)	Phosphorylation	-	26.43	27251275
32	Phosphorylation	VSKGPLNSESSNQSL CCCCCCCCCCCCCCC	46.68	25159151
34	Phosphorylation	KGPLNSESSNQSLCS CCCCCCCCCCCCCCC	34.12	25159151
35	Phosphorylation	GPLNSESSNQSLCSV CCCCCCCCCCCCCCC	34.53	25159151
35 (in isoform 2)	Phosphorylation	-	34.53	27251275
38	Phosphorylation	NSESSNQSLCSVGSL CCCCCCCCCCCCCCC	35.28	25159151
38 (in isoform 2)	Phosphorylation	-	35.28	27251275
41	Phosphorylation	SSNQSLCSVGSLSDK CCCCCCCCCCCCCHH	34.50	25159151
44	Phosphorylation	QSLCSVGSLSDKEVE CCCCCCCCCCHHCCC	23.65	25159151
44 (in isoform 2)	Phosphorylation	-	23.65	27251275
46	Phosphorylation	LCSVGSLSDKEVETP CCCCCCCCHHCCCCC	48.62	25159151
46 (in isoform 2)	Phosphorylation	-	48.62	27251275
52	Phosphorylation	LSDKEVETPEKKQND CCHHCCCCCHHHHHH	41.76	25022875
70	Phosphorylation	RKRKAEPYETSQGKG HHHCCCCCCCCCCCC	24.28	20873877
72	Phosphorylation	RKAEPYETSQGKGTP HCCCCCCCCCCCCCC	22.10	26055452
73	Phosphorylation	KAEPYETSQGKGTPR CCCCCCCCCCCCCCC	25.62	25159151
73 (in isoform 2)	Phosphorylation	-	25.62	24719451
78	Phosphorylation	ETSQGKGTPRGHKIS CCCCCCCCCCCCCCC	17.19	25159151
78 (in isoform 2)	Phosphorylation	-	17.19	24719451
78 (in isoform 3)	Phosphorylation	-	17.19	25159151
85	Phosphorylation	TPRGHKISDYFEFAG CCCCCCCCCCEEECC	30.94	28796482
87	Phosphorylation	RGHKISDYFEFAGGS CCCCCCCCEEECCCC	9.75	28796482
94	Phosphorylation	YFEFAGGSAPGTSPG CEEECCCCCCCCCCC	30.30	22167270
94 (in isoform 2)	Phosphorylation	-	30.30	24719451
98	Phosphorylation	AGGSAPGTSPGRSVP CCCCCCCCCCCCCCC	30.59	29255136
98 (in isoform 2)	Phosphorylation	-	30.59	24719451
99	Phosphorylation	GGSAPGTSPGRSVPP CCCCCCCCCCCCCCC	31.13	29255136
99 (in isoform 2)	Phosphorylation	-	31.13	27251275
102 (in isoform 3)	Phosphorylation	-	37.55	25159151
103	Phosphorylation	PGTSPGRSVPPVARS CCCCCCCCCCCCCCC	44.91	-
110	Phosphorylation	SVPPVARSSPQHSLS CCCCCCCCCCCCCCC	36.31	23927012
110 (in isoform 2)	Phosphorylation	-	36.31	-
111	Phosphorylation	VPPVARSSPQHSLSN CCCCCCCCCCCCCCC	24.03	23401153
111 (in isoform 2)	Phosphorylation	-	24.03	24719451
115	Phosphorylation	ARSSPQHSLSNPLPR CCCCCCCCCCCCCCC	28.39	25159151
115 (in isoform 2)	Phosphorylation	-	28.39	24719451
117	Phosphorylation	SSPQHSLSNPLPRRV CCCCCCCCCCCCCCC	39.14	23401153
117 (in isoform 2)	Phosphorylation	-	39.14	24719451
124 (in isoform 3)	Ubiquitination	-	9.40	21906983
129	Phosphorylation	RRVEQPLYGLDGSAA CCCCCCCCCCCCHHH	23.48	23403867
134	Phosphorylation	PLYGLDGSAAKEATE CCCCCCCHHHHHHHH	25.39	30266825
134 (in isoform 2)	Phosphorylation	-	25.39	27251275
140	Phosphorylation	GSAAKEATEEQSALP CHHHHHHHHHHHHHH	40.94	25849741
140 (in isoform 2)	Phosphorylation	-	40.94	27251275
144	Phosphorylation	KEATEEQSALPTLMS HHHHHHHHHHHHHHH	34.74	29514088
144 (in isoform 2)	Phosphorylation	-	34.74	27251275
148	O-linked_Glycosylation	EEQSALPTLMSVMLA HHHHHHHHHHHHHHH	35.62	31492838
148	Phosphorylation	EEQSALPTLMSVMLA HHHHHHHHHHHHHHH	35.62	29514088
156	Ubiquitination	LMSVMLAKPRLDTEQ HHHHHHHCCCCCHHH	27.06	2190698
156 (in isoform 1)	Ubiquitination	-	27.06	21906983
156 (in isoform 2)	Ubiquitination	-	27.06	21906983
174	Phosphorylation	RGAGLCFTFVSAQQN CCCCCEEEEEEECCC	23.33	27080861
177	Phosphorylation	GLCFTFVSAQQNSPS CCEEEEEEECCCCCC	19.03	30576142
177 (in isoform 2)	Phosphorylation	-	19.03	24719451
182	Phosphorylation	FVSAQQNSPSSTGSG EEEECCCCCCCCCCC	23.09	21712546
182 (in isoform 2)	Phosphorylation	-	23.09	27251275
184	Phosphorylation	SAQQNSPSSTGSGNT EECCCCCCCCCCCCC	40.11	27080861
185	Phosphorylation	AQQNSPSSTGSGNTE ECCCCCCCCCCCCCC	39.92	30576142
186	Phosphorylation	QQNSPSSTGSGNTEH CCCCCCCCCCCCCCC	39.44	27080861
188	Phosphorylation	NSPSSTGSGNTEHSC CCCCCCCCCCCCCCC	29.02	27080861
191	Phosphorylation	SSTGSGNTEHSCSSQ CCCCCCCCCCCCCCC	38.30	27080861
194	Phosphorylation	GSGNTEHSCSSQKQI CCCCCCCCCCCCCEE	14.95	27080861
196	Phosphorylation	GNTEHSCSSQKQISI CCCCCCCCCCCEEEE	38.53	27251275
197	Phosphorylation	NTEHSCSSQKQISIQ CCCCCCCCCCEEEEE	45.80	27080861
218	Phosphorylation	DLTIEKISALENSKN CCHHHHHHHHHHCCC	37.56	29759185
223	Phosphorylation	KISALENSKNSDLEK HHHHHHHCCCCHHHH	24.68	29759185
224	Ubiquitination	ISALENSKNSDLEKK HHHHHHCCCCHHHHH	72.55	-
226	Phosphorylation	ALENSKNSDLEKKEG HHHHCCCCHHHHHCC	47.51	25278378
259	Phosphorylation	QQKMLEKYKERLNRC HHHHHHHHHHHHHHH	14.54	22817900
268	Phosphorylation	ERLNRCVTMSKKLLI HHHHHHHHHHHHHHH	21.25	29759185
270	Phosphorylation	LNRCVTMSKKLLIEK HHHHHHHHHHHHHHH	19.78	29759185
300	Phosphorylation	RLRLGHFTTVRHGAS HHHHCCCEECCCCCC	20.78	23917254
301	Phosphorylation	LRLGHFTTVRHGASF HHHCCCEECCCCCCC	18.01	23186163
307	Phosphorylation	TTVRHGASFTEQWTD EECCCCCCCCCCCCC	36.98	28450419
309	Phosphorylation	VRHGASFTEQWTDGY CCCCCCCCCCCCCHH	25.44	28450419
309 (in isoform 2)	Phosphorylation	-	25.44	27251275
313	Phosphorylation	ASFTEQWTDGYAFQN CCCCCCCCCHHHHHH	21.17	28450419
316	Phosphorylation	TEQWTDGYAFQNLIK CCCCCCHHHHHHHHH	13.67	28450419
357	Phosphorylation	MGQAPPATNEQKQRK CCCCCCCCHHHHHHH	45.09	27470641
393	Phosphorylation	APALGAHSLLRLTLA HHHCCHHHHHHHHHH	28.68	24719451
418	Ubiquitination	LRLGHLKKEEAEIQA HHHCCCCHHHHHHHH	68.12	-
450	Phosphorylation	RIHNEDNSQFKDHPT HHHCCCCCHHCCCCC	49.68	23312004
491	Ubiquitination	EQRYVAVKIHQLNKN CCHHHEEEHHHHCCC	25.41	-
497	Ubiquitination	VKIHQLNKNWRDEKK EEHHHHCCCCCHHHH	67.49	-
519	Ubiquitination	CREYRIHKELDHPRI HHHHHHHHHCCCCCE	58.56	-
565	Acetylation	QHKLMSEKEARSIIM HHCCCCHHHHHHHHH	49.74	25953088
617	Phosphorylation	KITDFGLSKIMDDDS EEECCCCCCCCCCCC	22.10	21815630
679	Acetylation	YQCLYGRKPFGHNQS HHHHHCCCCCCCCHH	40.09	26051181
686	Phosphorylation	KPFGHNQSQQDILQE CCCCCCHHHHHHHHH	34.82	20873877
695	Phosphorylation	QDILQENTILKATEV HHHHHHCCCEEEEEC	27.25	20071362
724	Phosphorylation	FIRRCLAYRKEDRID HHHHHHHHCCCCCCC	14.85	22817900
731 (in isoform 2)	Phosphorylation	-	32.82	-
739 (in isoform 2)	Phosphorylation	-	22.89	24719451
740 (in isoform 2)	Phosphorylation	-	13.97	27251275
748	Phosphorylation	LLPHIRKSVSTSSPA HHHHHHHHCCCCCCC	16.08	23403867
748 (in isoform 2)	Phosphorylation	-	16.08	27251275
749 (in isoform 2)	Phosphorylation	-	8.97	27251275
750	Phosphorylation	PHIRKSVSTSSPAGA HHHHHHCCCCCCCCC	29.66	25159151
751	Phosphorylation	HIRKSVSTSSPAGAA HHHHHCCCCCCCCCH	30.94	25627689
752	Phosphorylation	IRKSVSTSSPAGAAI HHHHCCCCCCCCCHH	27.15	25262027
753	Phosphorylation	RKSVSTSSPAGAAIA HHHCCCCCCCCCHHH	20.89	25159151
761	Phosphorylation	PAGAAIASTSGASNN CCCCHHHCCCCCCCC	19.59	21712546
762	Phosphorylation	AGAAIASTSGASNNS CCCHHHCCCCCCCCC	23.35	23403867
763	Phosphorylation	GAAIASTSGASNNSS CCHHHCCCCCCCCCC	29.84	23403867
766	Phosphorylation	IASTSGASNNSSSN- HHCCCCCCCCCCCC-	39.82	22115753
769	Phosphorylation	TSGASNNSSSN---- CCCCCCCCCCC----	39.12	25159151
770	Phosphorylation	SGASNNSSSN----- CCCCCCCCCC-----	35.70	25159151
771	Phosphorylation	GASNNSSSN------ CCCCCCCCC------	46.51	25159151

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
686	S	Phosphorylation	Kinase	CHEK1	O14757	GPS
750	S	Phosphorylation	Kinase	CHEK1	O14757	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
750	S	Phosphorylation		12955071
Phosphorylated at Ser-750, probably by CHEK1.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of TLK2_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
ASF1A_HUMAN	ASF1A	physical	11470414
ASF1B_HUMAN	ASF1B	physical	11470414
TLK1_HUMAN	TLK1	physical	10523312
ASF1B_HUMAN	ASF1B	physical	17940067
ASF1A_HUMAN	ASF1A	physical	20016786
ASF1B_HUMAN	ASF1B	physical	20016786
XPO7_HUMAN	XPO7	physical	22939629
XPO1_HUMAN	XPO1	physical	22939629
TLK1_HUMAN	TLK1	physical	28514442
RS27A_HUMAN	RPS27A	physical	28514442
CABIN_HUMAN	CABIN1	physical	28514442
AMOL1_HUMAN	AMOTL1	physical	27173435
IFFO1_HUMAN	IFFO1	physical	27173435
CDAC1_HUMAN	CDADC1	physical	27173435
SCML1_HUMAN	SCML1	physical	27173435
COBL1_HUMAN	COBLL1	physical	27173435

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of TLK2_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions."; Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.; Sci. Signal. 2:RA46-RA46(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-99, AND MASSSPECTROMETRY.	19690332 [Abstract]
"Large-scale proteomics analysis of the human kinome."; Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G.,Mann M., Daub H.; Mol. Cell. Proteomics 8:1751-1764(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-32; TYR-70; THR-72;SER-73; SER-94; THR-98; SER-99; SER-110; SER-111; SER-115; SER-117;SER-134; SER-196; THR-300; SER-769; SER-770 AND SER-771, AND MASSSPECTROMETRY.	19369195 [Abstract]
"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach."; Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.; Anal. Chem. 81:4493-4501(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-99; SER-111; SER-115 ANDSER-771, AND MASS SPECTROMETRY.	19413330 [Abstract]
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-99, AND MASSSPECTROMETRY.	18669648 [Abstract]
"Kinase-selective enrichment enables quantitative phosphoproteomics ofthe kinome across the cell cycle."; Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R.,Greff Z., Keri G., Stemmann O., Mann M.; Mol. Cell 31:438-448(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-99 AND SER-753, AND MASSSPECTROMETRY.	18691976 [Abstract]
"Large-scale characterization of HeLa cell nuclear phosphoproteins."; Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J.,Li J., Cohn M.A., Cantley L.C., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-99, AND MASSSPECTROMETRY.	15302935 [Abstract]

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