dbPTM

TLK1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	TLK1_HUMAN
UniProt AC	Q9UKI8
Protein Name	Serine/threonine-protein kinase tousled-like 1
Gene Name	TLK1
Organism	Homo sapiens (Human).
Sequence Length	766
Subcellular Localization	Nucleus .
Protein Description	Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly. Isoform 3 phosphorylates and enhances the stability of the t-SNARE SNAP23, augmenting its assembly with syntaxin. Isoform 3 protects the cells from the ionizing radiation by facilitating the repair of DSBs. In vitro, phosphorylates histone H3 at 'Ser-10'..
Protein Sequence	MSVQSSSGSLEGPPSWSQLSTSPTPGSAAAARSLLNHTPPSGRPREGAMDELHSLDPRRQELLEARFTGVASGSTGSTGSCSVGAKASTNNESSNHSFGSLGSLSDKESETPEKKQSESSRGRKRKAENQNESSQGKSIGGRGHKISDYFEYQGGNGSSPVRGIPPAIRSPQNSHSHSTPSSSVRPNSPSPTALAFGDHPIVQPKQLSFKIIQTDLTMLKLAALESNKIQDLEKKEGRIDDLLRANCDLRRQIDEQQKLLEKYKERLNKCISMSKKLLIEKSTQEKLSSREKSMQDRLRLGHFTTVRHGASFTEQWTDGFAFQNLVKQQEWVNQQREDIERQRKLLAKRKPPTANNSQAPSTNSEPKQRKNKAVNGAENDPFVRPNLPQLLTLAEYHEQEEIFKLRLGHLKKEEAEIQAELERLERVRNLHIRELKRINNEDNSQFKDHPTLNERYLLLHLLGRGGFSEVYKAFDLYEQRYAAVKIHQLNKSWRDEKKENYHKHACREYRIHKELDHPRIVKLYDYFSLDTDTFCTVLEYCEGNDLDFYLKQHKLMSEKEARSIVMQIVNALRYLNEIKPPIIHYDLKPGNILLVDGTACGEIKITDFGLSKIMDDDSYGVDGMDLTSQGAGTYWYLPPECFVVGKEPPKISNKVDVWSVGVIFFQCLYGRKPFGHNQSQQDILQENTILKATEVQFPVKPVVSSEAKAFIRRCLAYRKEDRFDVHQLANDPYLLPHMRRSNSSGNLHMAGLTASPTPPSSSIITY

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1 (in isoform 3)	Acetylation	-	8.81	22814378
7	Phosphorylation	-MSVQSSSGSLEGPP -CCCCCCCCCCCCCC	37.33	24719451
9	Phosphorylation	SVQSSSGSLEGPPSW CCCCCCCCCCCCCCH	25.79	22210691
11 (in isoform 4)	Phosphorylation	-	71.94	27762562
15 (in isoform 4)	Phosphorylation	-	42.36	27762562
20	Phosphorylation	PPSWSQLSTSPTPGS CCCHHHCCCCCCCCC	21.14	28348404
21	Phosphorylation	PSWSQLSTSPTPGSA CCHHHCCCCCCCCCH	47.52	24719451
22	Phosphorylation	SWSQLSTSPTPGSAA CHHHCCCCCCCCCHH	24.33	25159151
24	Phosphorylation	SQLSTSPTPGSAAAA HHCCCCCCCCCHHHH	39.80	27251275
33	Phosphorylation	GSAAAARSLLNHTPP CCHHHHHHHHHCCCC	32.27	22617229
34	Phosphorylation	SAAAARSLLNHTPPS CHHHHHHHHHCCCCC	4.65	33259812
38	Phosphorylation	ARSLLNHTPPSGRPR HHHHHHCCCCCCCCC	35.50	23401153
41	Phosphorylation	LLNHTPPSGRPREGA HHHCCCCCCCCCCCC	48.95	25159151
54	Phosphorylation	GAMDELHSLDPRRQE CCHHHHHHCCHHHHH	47.28	23917254
68	Phosphorylation	ELLEARFTGVASGST HHHHHHHCCCCCCCC	25.78	30108239
72	Phosphorylation	ARFTGVASGSTGSTG HHHCCCCCCCCCCCC	30.76	30177828
74	Phosphorylation	FTGVASGSTGSTGSC HCCCCCCCCCCCCCC	27.40	25159151
75	Phosphorylation	TGVASGSTGSTGSCS CCCCCCCCCCCCCCC	38.38	25159151
77	Phosphorylation	VASGSTGSTGSCSVG CCCCCCCCCCCCCCC	29.68	25159151
78	Phosphorylation	ASGSTGSTGSCSVGA CCCCCCCCCCCCCCC	34.16	25159151
80	Phosphorylation	GSTGSTGSCSVGAKA CCCCCCCCCCCCCEE	11.87	25159151
82	Phosphorylation	TGSTGSCSVGAKAST CCCCCCCCCCCEECC	25.86	25159151
88	Phosphorylation	CSVGAKASTNNESSN CCCCCEECCCCCCCC	30.84	23927012
89	Phosphorylation	SVGAKASTNNESSNH CCCCEECCCCCCCCC	47.09	29255136
93	Phosphorylation	KASTNNESSNHSFGS EECCCCCCCCCCCCC	38.27	29255136
94	Phosphorylation	ASTNNESSNHSFGSL ECCCCCCCCCCCCCC	32.17	29255136
97	Phosphorylation	NNESSNHSFGSLGSL CCCCCCCCCCCCCCC	35.24	29255136
100	Phosphorylation	SSNHSFGSLGSLSDK CCCCCCCCCCCCCCC	27.78	30576142
103	Phosphorylation	HSFGSLGSLSDKESE CCCCCCCCCCCCCCC	29.96	30576142
105	Phosphorylation	FGSLGSLSDKESETP CCCCCCCCCCCCCCC	48.62	25159151
109	Phosphorylation	GSLSDKESETPEKKQ CCCCCCCCCCCHHHH	53.14	28985074
111	Phosphorylation	LSDKESETPEKKQSE CCCCCCCCCHHHHCC	46.13	23927012
133	Phosphorylation	KAENQNESSQGKSIG HHHHCCCCCCCCCCC	35.20	25849741
134	Phosphorylation	AENQNESSQGKSIGG HHHCCCCCCCCCCCC	36.45	30576142
138	Phosphorylation	NESSQGKSIGGRGHK CCCCCCCCCCCCCCC	33.03	20873877
147	Phosphorylation	GGRGHKISDYFEYQG CCCCCCCCCCEEECC	30.94	25219547
149	Phosphorylation	RGHKISDYFEYQGGN CCCCCCCCEEECCCC	7.48	25219547
152	Phosphorylation	KISDYFEYQGGNGSS CCCCCEEECCCCCCC	11.54	23927012
158	Phosphorylation	EYQGGNGSSPVRGIP EECCCCCCCCCCCCC	35.15	23401153
159	Phosphorylation	YQGGNGSSPVRGIPP ECCCCCCCCCCCCCC	28.90	19664994
162	Ubiquitination	GNGSSPVRGIPPAIR CCCCCCCCCCCCCCC	40.48	30230243
170	Phosphorylation	GIPPAIRSPQNSHSH CCCCCCCCCCCCCCC	25.40	23927012
174	Phosphorylation	AIRSPQNSHSHSTPS CCCCCCCCCCCCCCC	22.56	23927012
176	Phosphorylation	RSPQNSHSHSTPSSS CCCCCCCCCCCCCCC	20.96	23927012
178	Phosphorylation	PQNSHSHSTPSSSVR CCCCCCCCCCCCCCC	45.06	23927012
179	Phosphorylation	QNSHSHSTPSSSVRP CCCCCCCCCCCCCCC	23.05	23927012
181	Phosphorylation	SHSHSTPSSSVRPNS CCCCCCCCCCCCCCC	35.02	23927012
182	Phosphorylation	HSHSTPSSSVRPNSP CCCCCCCCCCCCCCC	33.77	23401153
183	Phosphorylation	SHSTPSSSVRPNSPS CCCCCCCCCCCCCCC	26.87	23927012
188	Phosphorylation	SSSVRPNSPSPTALA CCCCCCCCCCCCEEE	29.45	23401153
190	Phosphorylation	SVRPNSPSPTALAFG CCCCCCCCCCEEECC	34.40	23927012
192	Phosphorylation	RPNSPSPTALAFGDH CCCCCCCCEEECCCC	38.34	23927012
208	Phosphorylation	IVQPKQLSFKIIQTD CCCCCCCCEEEEHHH	23.23	24719451
210	Ubiquitination	QPKQLSFKIIQTDLT CCCCCCEEEEHHHHH	35.21	30230243
230	Ubiquitination	ALESNKIQDLEKKEG HHHCCCCHHHHHHCC	51.34	24816145
234	Acetylation	NKIQDLEKKEGRIDD CCCHHHHHHCCCHHH	64.52	18586057
258	Ubiquitination	RQIDEQQKLLEKYKE HHHHHHHHHHHHHHH	55.52	30230243
304	Phosphorylation	RLRLGHFTTVRHGAS HHHHCCCCCCCCCCC	20.78	23917254
305	Phosphorylation	LRLGHFTTVRHGASF HHHCCCCCCCCCCCC	18.01	23186163
311	Phosphorylation	TTVRHGASFTEQWTD CCCCCCCCCCCCCCC	36.98	28450419
313	Phosphorylation	VRHGASFTEQWTDGF CCCCCCCCCCCCCCC	25.44	28450419
316	Ubiquitination	GASFTEQWTDGFAFQ CCCCCCCCCCCCHHH	7.10	29967540
317	Phosphorylation	ASFTEQWTDGFAFQN CCCCCCCCCCCHHHH	26.41	28450419
351	Ubiquitination	KLLAKRKPPTANNSQ HHHHHCCCCCCCCCC	35.19	24816145
353	Phosphorylation	LAKRKPPTANNSQAP HHHCCCCCCCCCCCC	51.01	25262027
357	Phosphorylation	KPPTANNSQAPSTNS CCCCCCCCCCCCCCC	27.76	25627689
361	Phosphorylation	ANNSQAPSTNSEPKQ CCCCCCCCCCCCCHH	43.14	30619164
362	Phosphorylation	NNSQAPSTNSEPKQR CCCCCCCCCCCCHHH	41.53	30619164
364	Phosphorylation	SQAPSTNSEPKQRKN CCCCCCCCCCHHHHC	56.20	23312004
364	Ubiquitination	SQAPSTNSEPKQRKN CCCCCCCCCCHHHHC	56.20	29967540
384	Ubiquitination	AENDPFVRPNLPQLL CCCCCCCCCCHHHHH	18.12	24816145
389	Ubiquitination	FVRPNLPQLLTLAEY CCCCCHHHHHHHHHH	52.88	29967540
399	Ubiquitination	TLAEYHEQEEIFKLR HHHHHHHHHHHHHHH	40.07	24816145
412	Ubiquitination	LRLGHLKKEEAEIQA HHHCCCCHHHHHHHH	68.12	29967540
417	Ubiquitination	LKKEEAEIQAELERL CCHHHHHHHHHHHHH	6.40	29967540
437	Ubiquitination	LHIRELKRINNEDNS HHHHHHHHHCCCCCC	49.87	29967540
444	Phosphorylation	RINNEDNSQFKDHPT HHCCCCCCHHCCCCC	49.68	23312004
447	Ubiquitination	NEDNSQFKDHPTLNE CCCCCHHCCCCCHHH	47.71	24816145
451	Phosphorylation	SQFKDHPTLNERYLL CHHCCCCCHHHHHHH	38.50	23403867
465	Ubiquitination	LLHLLGRGGFSEVYK HHHHHHCCCHHHHHH	39.43	29967540
483	Ubiquitination	LYEQRYAAVKIHQLN HHHHHHHHHHHHHHC	8.32	23000965
485	Ubiquitination	EQRYAAVKIHQLNKS HHHHHHHHHHHHCHH	29.73	29967540
513	Ubiquitination	CREYRIHKELDHPRI HHHHHHHHHCCCCCE	58.56	29967540
563	Phosphorylation	MSEKEARSIVMQIVN CCHHHHHHHHHHHHH	26.95	-
604	Ubiquitination	GTACGEIKITDFGLS CCCCCEEEEECCCCC	34.94	23000965
611	Phosphorylation	KITDFGLSKIMDDDS EEECCCCCCCCCCCC	22.10	21815630
637	Ubiquitination	GAGTYWYLPPECFVV CCCCEEECCCHHEEE	3.36	23000965
652	Ubiquitination	GKEPPKISNKVDVWS CCCCCCCCCCCCHHH	36.40	23000965
679	Phosphorylation	KPFGHNQSQQDILQE CCCCCCHHHHHHHHH	34.82	20873877
688	Phosphorylation	QDILQENTILKATEV HHHHHHCCCEEEEEE	27.25	20071362
700	Ubiquitination	TEVQFPVKPVVSSEA EEEECCCCCCCCHHH	32.12	23000965
717	Phosphorylation	FIRRCLAYRKEDRFD HHHHHHHHCCCCCCC	14.85	-
721 (in isoform 2)	Ubiquitination	-	60.63	-
741	Phosphorylation	LLPHMRRSNSSGNLH HHHCHHCCCCCCCCE	30.60	30278072
743	Phosphorylation	PHMRRSNSSGNLHMA HCHHCCCCCCCCEEC	40.65	30278072
744	Phosphorylation	HMRRSNSSGNLHMAG CHHCCCCCCCCEECC	35.63	30278072
753	Phosphorylation	NLHMAGLTASPTPPS CCEECCEECCCCCCC	24.95	30278072
755	Phosphorylation	HMAGLTASPTPPSSS EECCEECCCCCCCHH	24.87	26055452
757	Phosphorylation	AGLTASPTPPSSSII CCEECCCCCCCHHCC	45.39	23403867
760	Phosphorylation	TASPTPPSSSIITY- ECCCCCCCHHCCCC-	37.61	23403867
761	Phosphorylation	ASPTPPSSSIITY-- CCCCCCCHHCCCC--	31.19	23403867
762	Phosphorylation	SPTPPSSSIITY--- CCCCCCHHCCCC---	23.50	23403867
765	Phosphorylation	PPSSSIITY------ CCCHHCCCC------	22.85	23403867
766	Phosphorylation	PSSSIITY------- CCHHCCCC-------	13.22	23403867
783 (in isoform 2)	Phosphorylation	-		18669648

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
679	S	Phosphorylation	Kinase	CHEK1	O14757	GPS
743	S	Phosphorylation	Kinase	CHEK1	O14757	GPS

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of TLK1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of TLK1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
ASF1A_HUMAN	ASF1A	physical	11470414
ASF1B_HUMAN	ASF1B	physical	11470414
TLK1_HUMAN	TLK1	physical	16189514
ASF1A_HUMAN	ASF1A	physical	16189514
TLK1_HUMAN	TLK1	physical	10523312
TLK2_HUMAN	TLK2	physical	10523312
ASF1A_HUMAN	ASF1A	physical	17940067
TLK1_HUMAN	TLK1	physical	12955071
MBP_HUMAN	MBP	physical	12955071
NBN_HUMAN	NBN	physical	12955071
ASF1A_HUMAN	ASF1A	physical	12660173
XPO7_HUMAN	XPO7	physical	22939629
UBE3C_HUMAN	UBE3C	physical	21988832
TLK1_HUMAN	TLK1	physical	25416956
LUZP4_HUMAN	LUZP4	physical	25416956
K1468_HUMAN	KIAA1468	physical	27173435

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of TLK1_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions."; Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.; Sci. Signal. 2:RA46-RA46(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-159 AND SER-741, ANDMASS SPECTROMETRY.	19690332 [Abstract]
"Large-scale proteomics analysis of the human kinome."; Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G.,Mann M., Daub H.; Mol. Cell. Proteomics 8:1751-1764(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-74; THR-75; SER-77;THR-78; SER-80; SER-133; SER-138; SER-158; SER-159 AND SER-741, ANDMASS SPECTROMETRY.	19369195 [Abstract]
"Global, in vivo, and site-specific phosphorylation dynamics insignaling networks."; Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P.,Mann M.; Cell 127:635-648(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-179 AND SER-190, ANDMASS SPECTROMETRY.	17081983 [Abstract]
"Large-scale characterization of HeLa cell nuclear phosphoproteins."; Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J.,Li J., Cohn M.A., Cantley L.C., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-159, AND MASSSPECTROMETRY.	15302935 [Abstract]
"Human tousled like kinases are targeted by an ATM- and Chk1-dependentDNA damage checkpoint."; Groth A., Lukas J., Nigg E.A., Sillje H.H.W., Wernstedt C., Bartek J.,Hansen K.; EMBO J. 22:1676-1687(2003). Cited for: FUNCTION, MUTAGENESIS OF SER-743, PHOSPHORYLATION AT SER-743, ANDENZYME REGULATION.	12660173 [Abstract]